Immobilized stem–loop structured probes as conformational switches for enzymatic detection of microbial 16S rRNA

We have designed and evaluated novel DNA stem–loop structured probes for enzymatic detection of nucleic acid targets. These probes constitute a novel class of conformational switches for enzymatic activity, which in the absence of a target sterically shield an affinity label and upon hybridization o...

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Veröffentlicht in:	Nucleic acids research 2005-01, Vol.33 (11), p.e101-e101
Hauptverfasser:	Bockisch, Benjamin, Grunwald, Thomas, Spillner, Edzard, Bredehorst, Reinhard
Format:	Artikel
Sprache:	eng
Schlagworte:	Colorimetry DNA Probes - chemistry Enzymes Methods Online Nucleic Acid Conformation RNA, Bacterial - analysis RNA, Ribosomal, 16S - analysis
Online-Zugang:	Volltext
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