The rapid purification of 3-hydroxybutyrate dehydrogenase and malate dehydrogenase on triazine dye affinity matrices

3-Hydroxybutyrate dehydrogenase (EC 1.1.1.30) and malate dehydrogenase (EC 1.1.1.37) were purified to homogeneity on a large scale involving only two sequential affinity-chromatography steps on two triazine dye-Sepharose matrices. Recoveries of both enzymes were in excess of 60%. Malate dehydrogenas...

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Veröffentlicht in:	Biochemical journal 1982-06, Vol.203 (3), p.699-705
Hauptverfasser:	Scawen, M D, Darbyshire, J, Harvey, M J, Atkinson, T
Format:	Artikel
Sprache:	eng
Schlagworte:	3-hydroxybutyrate dehydrogenase Chromatography, Affinity - methods Chromatography, Gel Coloring Agents Hydroxybutyrate Dehydrogenase - isolation & purification malate dehydrogenase Malate Dehydrogenase - isolation & purification methodology purification Rhodobacter sphaeroides - enzymology Rhodopseudomonas sphaeroides Sepharose Triazines
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