Functional characterization of OR51B5 and OR1G1 in human lung epithelial cells as potential drug targets for non-type 2 lung diseases
Background Hypersensitivity to odorants like perfumes can induce or promote asthma with non-type 2 inflammation for which therapeutic options are limited. Cell death of primary bronchial epithelial cells (PBECs) and the release of the pro-inflammatory cytokines interleukin-6 (IL-6) and IL-8 are key...
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| Veröffentlicht in: | Cell biology and toxicology 2024-11, Vol.40 (1), p.96, Article 96 |
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| creator | Awad, Noha Weidinger, Daniel Greune, Lea Kronsbein, Juliane Heinen, Natalie Westhoven, Saskia Pfaender, Stephanie Taube, Christian Reuter, Sebastian Peters, Marcus Hatt, Hanns Fender, Anke Knobloch, Jürgen |
| description | Background
Hypersensitivity to odorants like perfumes can induce or promote asthma with non-type 2 inflammation for which therapeutic options are limited. Cell death of primary bronchial epithelial cells (PBECs) and the release of the pro-inflammatory cytokines interleukin-6 (IL-6) and IL-8 are key in the pathogenesis. Extra-nasal olfactory receptors (ORs) can influence cellular processes involved in asthma. This study investigated the utility of ORs in epithelial cells as potential drug targets in this context.
Methods
We used the A549 cell line and primary bronchial epithelial cells using air–liquid interface culture system (ALI-PBECs). OR expression was investigated by RT-PCR, Western blot, and Immunofluorescence. Effects of OR activation by specific ligands on intracellular calcium concentration, cAMP, Phospholipase C (PLC), cell viability, and IL-6 and IL-8 secretion were analyzed by calcium imaging, enzyme immunoassays, Annexin V/ propidium iodide -based fluorescence-activated cell staining or by ELISA, respectively.
Results
By screening A549 cells, the OR51B5 agonists Farnesol and Isononyl Alcohol and the OR1G1 agonist Nonanal increased intracellular Ca2 + . OR51B5 and OR1G1 mRNAs and proteins were detected. Both receptors showed a preferential intracellular localization. OR51B5- but not OR1G1-induced Ca2 + dependent on both cAMP and PLC signaling. Farnesol, Isononyl Alcohol, and Nonanal, all reduced cell viability and induced IL-8 and IL-6 release. The data were verified in ALI-PBECs.
Conclusion
ORs in the lung epithelium might be involved in airway-sensitivity to odorants. Their antagonism could represent a promising strategy in treatment of odorant-induced asthma with non-type 2 inflammation.
Graphical Abstract |
| doi_str_mv | 10.1007/s10565-024-09935-9 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_11561009</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>3128039205</sourcerecordid><originalsourceid>FETCH-LOGICAL-c356t-a10f0fa4980ffbf649f4caa8f5d8355b6e165510f14021fb13fe866f759b81733</originalsourceid><addsrcrecordid>eNp9kU9vFiEQxonR2D_6BTwYEi9eVpllYeFktLHVpEmTRs-E3YV9afaFFdgm9e73lu3WWj30xDDzm4cZHoReAXkHhLTvExDGWUXqpiJSUlbJJ-gQWEsrLur66YP4AB2ldEUI4dCy5-iASkZFuRyiX6eL77MLXk-43-mo-2yi-6nXFA4WX1wy-MSw9kMJ4Qyw83i37LXH0-JHbGaXd2Zya7eZpoR1wnPIxuc1NcRlxFnH0eSEbYjYB1_lm9ngemsfXDI6mfQCPbN6Subl3XmMvp9-_nbypTq_OPt68vG86injudJALLG6kYJY21neSNv0WgvLBkEZ67gBzliBoCE12A6oNYJz2zLZCWgpPUYfNt156fZm6MucUU9qjm6v440K2ql_K97t1BiuFQDj5c9lUXh7pxDDj8WkrPYuratrb8KSFIVaCJAUWEHf_IdehSWWj94oQmVNVqreqD6GlKKx99MAUavNarNZFZvVrc1qneL1wz3uW_74WgC6AamU_Gji37cfkf0NI2mzrg</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>3128039205</pqid></control><display><type>article</type><title>Functional characterization of OR51B5 and OR1G1 in human lung epithelial cells as potential drug targets for non-type 2 lung diseases</title><source>MEDLINE</source><source>SpringerLink Journals</source><source>Alma/SFX Local Collection</source><creator>Awad, Noha ; Weidinger, Daniel ; Greune, Lea ; Kronsbein, Juliane ; Heinen, Natalie ; Westhoven, Saskia ; Pfaender, Stephanie ; Taube, Christian ; Reuter, Sebastian ; Peters, Marcus ; Hatt, Hanns ; Fender, Anke ; Knobloch, Jürgen</creator><creatorcontrib>Awad, Noha ; Weidinger, Daniel ; Greune, Lea ; Kronsbein, Juliane ; Heinen, Natalie ; Westhoven, Saskia ; Pfaender, Stephanie ; Taube, Christian ; Reuter, Sebastian ; Peters, Marcus ; Hatt, Hanns ; Fender, Anke ; Knobloch, Jürgen</creatorcontrib><description>Background
Hypersensitivity to odorants like perfumes can induce or promote asthma with non-type 2 inflammation for which therapeutic options are limited. Cell death of primary bronchial epithelial cells (PBECs) and the release of the pro-inflammatory cytokines interleukin-6 (IL-6) and IL-8 are key in the pathogenesis. Extra-nasal olfactory receptors (ORs) can influence cellular processes involved in asthma. This study investigated the utility of ORs in epithelial cells as potential drug targets in this context.
Methods
We used the A549 cell line and primary bronchial epithelial cells using air–liquid interface culture system (ALI-PBECs). OR expression was investigated by RT-PCR, Western blot, and Immunofluorescence. Effects of OR activation by specific ligands on intracellular calcium concentration, cAMP, Phospholipase C (PLC), cell viability, and IL-6 and IL-8 secretion were analyzed by calcium imaging, enzyme immunoassays, Annexin V/ propidium iodide -based fluorescence-activated cell staining or by ELISA, respectively.
Results
By screening A549 cells, the OR51B5 agonists Farnesol and Isononyl Alcohol and the OR1G1 agonist Nonanal increased intracellular Ca2 + . OR51B5 and OR1G1 mRNAs and proteins were detected. Both receptors showed a preferential intracellular localization. OR51B5- but not OR1G1-induced Ca2 + dependent on both cAMP and PLC signaling. Farnesol, Isononyl Alcohol, and Nonanal, all reduced cell viability and induced IL-8 and IL-6 release. The data were verified in ALI-PBECs.
Conclusion
ORs in the lung epithelium might be involved in airway-sensitivity to odorants. Their antagonism could represent a promising strategy in treatment of odorant-induced asthma with non-type 2 inflammation.
Graphical Abstract</description><identifier>ISSN: 1573-6822</identifier><identifier>ISSN: 0742-2091</identifier><identifier>EISSN: 1573-6822</identifier><identifier>DOI: 10.1007/s10565-024-09935-9</identifier><identifier>PMID: 39538061</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>A549 Cells ; Agonists ; Annexin V ; Aroma compounds ; Asthma ; Biochemistry ; Biomedical and Life Sciences ; Calcium ; Calcium (intracellular) ; Calcium - metabolism ; Calcium imaging ; Cell Biology ; Cell culture ; Cell death ; Cell Survival - drug effects ; Cell viability ; Cyclic AMP ; Cyclic AMP - metabolism ; Enzyme-linked immunosorbent assay ; Epithelial cells ; Epithelial Cells - drug effects ; Epithelial Cells - metabolism ; Epithelium ; Farnesol ; Humans ; Hypersensitivity ; Immunoassays ; Immunofluorescence ; Inflammation ; Interleukin 6 ; Interleukin 8 ; Interleukin-6 - genetics ; Interleukin-6 - metabolism ; Interleukin-8 - metabolism ; Intracellular ; Intracellular signalling ; Iodides ; Life Sciences ; Localization ; Lung - drug effects ; Lung - metabolism ; Lung - pathology ; Lung diseases ; Odorants ; Pathogenesis ; Perfumes ; Pharmacology/Toxicology ; Receptors ; Receptors, Odorant - genetics ; Receptors, Odorant - metabolism ; Therapeutic targets ; Type C Phospholipases - metabolism</subject><ispartof>Cell biology and toxicology, 2024-11, Vol.40 (1), p.96, Article 96</ispartof><rights>The Author(s) 2024</rights><rights>2024. The Author(s).</rights><rights>Copyright Springer Nature B.V. Dec 2024</rights><rights>The Author(s) 2024 2024</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c356t-a10f0fa4980ffbf649f4caa8f5d8355b6e165510f14021fb13fe866f759b81733</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10565-024-09935-9$$EPDF$$P50$$Gspringer$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10565-024-09935-9$$EHTML$$P50$$Gspringer$$Hfree_for_read</linktohtml><link.rule.ids>230,314,776,780,881,27903,27904,41467,42536,51297</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/39538061$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Awad, Noha</creatorcontrib><creatorcontrib>Weidinger, Daniel</creatorcontrib><creatorcontrib>Greune, Lea</creatorcontrib><creatorcontrib>Kronsbein, Juliane</creatorcontrib><creatorcontrib>Heinen, Natalie</creatorcontrib><creatorcontrib>Westhoven, Saskia</creatorcontrib><creatorcontrib>Pfaender, Stephanie</creatorcontrib><creatorcontrib>Taube, Christian</creatorcontrib><creatorcontrib>Reuter, Sebastian</creatorcontrib><creatorcontrib>Peters, Marcus</creatorcontrib><creatorcontrib>Hatt, Hanns</creatorcontrib><creatorcontrib>Fender, Anke</creatorcontrib><creatorcontrib>Knobloch, Jürgen</creatorcontrib><title>Functional characterization of OR51B5 and OR1G1 in human lung epithelial cells as potential drug targets for non-type 2 lung diseases</title><title>Cell biology and toxicology</title><addtitle>Cell Biol Toxicol</addtitle><addtitle>Cell Biol Toxicol</addtitle><description>Background
Hypersensitivity to odorants like perfumes can induce or promote asthma with non-type 2 inflammation for which therapeutic options are limited. Cell death of primary bronchial epithelial cells (PBECs) and the release of the pro-inflammatory cytokines interleukin-6 (IL-6) and IL-8 are key in the pathogenesis. Extra-nasal olfactory receptors (ORs) can influence cellular processes involved in asthma. This study investigated the utility of ORs in epithelial cells as potential drug targets in this context.
Methods
We used the A549 cell line and primary bronchial epithelial cells using air–liquid interface culture system (ALI-PBECs). OR expression was investigated by RT-PCR, Western blot, and Immunofluorescence. Effects of OR activation by specific ligands on intracellular calcium concentration, cAMP, Phospholipase C (PLC), cell viability, and IL-6 and IL-8 secretion were analyzed by calcium imaging, enzyme immunoassays, Annexin V/ propidium iodide -based fluorescence-activated cell staining or by ELISA, respectively.
Results
By screening A549 cells, the OR51B5 agonists Farnesol and Isononyl Alcohol and the OR1G1 agonist Nonanal increased intracellular Ca2 + . OR51B5 and OR1G1 mRNAs and proteins were detected. Both receptors showed a preferential intracellular localization. OR51B5- but not OR1G1-induced Ca2 + dependent on both cAMP and PLC signaling. Farnesol, Isononyl Alcohol, and Nonanal, all reduced cell viability and induced IL-8 and IL-6 release. The data were verified in ALI-PBECs.
Conclusion
ORs in the lung epithelium might be involved in airway-sensitivity to odorants. Their antagonism could represent a promising strategy in treatment of odorant-induced asthma with non-type 2 inflammation.
Graphical Abstract</description><subject>A549 Cells</subject><subject>Agonists</subject><subject>Annexin V</subject><subject>Aroma compounds</subject><subject>Asthma</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Calcium</subject><subject>Calcium (intracellular)</subject><subject>Calcium - metabolism</subject><subject>Calcium imaging</subject><subject>Cell Biology</subject><subject>Cell culture</subject><subject>Cell death</subject><subject>Cell Survival - drug effects</subject><subject>Cell viability</subject><subject>Cyclic AMP</subject><subject>Cyclic AMP - metabolism</subject><subject>Enzyme-linked immunosorbent assay</subject><subject>Epithelial cells</subject><subject>Epithelial Cells - drug effects</subject><subject>Epithelial Cells - metabolism</subject><subject>Epithelium</subject><subject>Farnesol</subject><subject>Humans</subject><subject>Hypersensitivity</subject><subject>Immunoassays</subject><subject>Immunofluorescence</subject><subject>Inflammation</subject><subject>Interleukin 6</subject><subject>Interleukin 8</subject><subject>Interleukin-6 - genetics</subject><subject>Interleukin-6 - metabolism</subject><subject>Interleukin-8 - metabolism</subject><subject>Intracellular</subject><subject>Intracellular signalling</subject><subject>Iodides</subject><subject>Life Sciences</subject><subject>Localization</subject><subject>Lung - drug effects</subject><subject>Lung - metabolism</subject><subject>Lung - pathology</subject><subject>Lung diseases</subject><subject>Odorants</subject><subject>Pathogenesis</subject><subject>Perfumes</subject><subject>Pharmacology/Toxicology</subject><subject>Receptors</subject><subject>Receptors, Odorant - genetics</subject><subject>Receptors, Odorant - metabolism</subject><subject>Therapeutic targets</subject><subject>Type C Phospholipases - metabolism</subject><issn>1573-6822</issn><issn>0742-2091</issn><issn>1573-6822</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>EIF</sourceid><recordid>eNp9kU9vFiEQxonR2D_6BTwYEi9eVpllYeFktLHVpEmTRs-E3YV9afaFFdgm9e73lu3WWj30xDDzm4cZHoReAXkHhLTvExDGWUXqpiJSUlbJJ-gQWEsrLur66YP4AB2ldEUI4dCy5-iASkZFuRyiX6eL77MLXk-43-mo-2yi-6nXFA4WX1wy-MSw9kMJ4Qyw83i37LXH0-JHbGaXd2Zya7eZpoR1wnPIxuc1NcRlxFnH0eSEbYjYB1_lm9ngemsfXDI6mfQCPbN6Subl3XmMvp9-_nbypTq_OPt68vG86injudJALLG6kYJY21neSNv0WgvLBkEZ67gBzliBoCE12A6oNYJz2zLZCWgpPUYfNt156fZm6MucUU9qjm6v440K2ql_K97t1BiuFQDj5c9lUXh7pxDDj8WkrPYuratrb8KSFIVaCJAUWEHf_IdehSWWj94oQmVNVqreqD6GlKKx99MAUavNarNZFZvVrc1qneL1wz3uW_74WgC6AamU_Gji37cfkf0NI2mzrg</recordid><startdate>20241113</startdate><enddate>20241113</enddate><creator>Awad, Noha</creator><creator>Weidinger, Daniel</creator><creator>Greune, Lea</creator><creator>Kronsbein, Juliane</creator><creator>Heinen, Natalie</creator><creator>Westhoven, Saskia</creator><creator>Pfaender, Stephanie</creator><creator>Taube, Christian</creator><creator>Reuter, Sebastian</creator><creator>Peters, Marcus</creator><creator>Hatt, Hanns</creator><creator>Fender, Anke</creator><creator>Knobloch, Jürgen</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>C6C</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7TM</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>K9.</scope><scope>NAPCQ</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20241113</creationdate><title>Functional characterization of OR51B5 and OR1G1 in human lung epithelial cells as potential drug targets for non-type 2 lung diseases</title><author>Awad, Noha ; Weidinger, Daniel ; Greune, Lea ; Kronsbein, Juliane ; Heinen, Natalie ; Westhoven, Saskia ; Pfaender, Stephanie ; Taube, Christian ; Reuter, Sebastian ; Peters, Marcus ; Hatt, Hanns ; Fender, Anke ; Knobloch, Jürgen</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c356t-a10f0fa4980ffbf649f4caa8f5d8355b6e165510f14021fb13fe866f759b81733</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>A549 Cells</topic><topic>Agonists</topic><topic>Annexin V</topic><topic>Aroma compounds</topic><topic>Asthma</topic><topic>Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Calcium</topic><topic>Calcium (intracellular)</topic><topic>Calcium - metabolism</topic><topic>Calcium imaging</topic><topic>Cell Biology</topic><topic>Cell culture</topic><topic>Cell death</topic><topic>Cell Survival - drug effects</topic><topic>Cell viability</topic><topic>Cyclic AMP</topic><topic>Cyclic AMP - metabolism</topic><topic>Enzyme-linked immunosorbent assay</topic><topic>Epithelial cells</topic><topic>Epithelial Cells - drug effects</topic><topic>Epithelial Cells - metabolism</topic><topic>Epithelium</topic><topic>Farnesol</topic><topic>Humans</topic><topic>Hypersensitivity</topic><topic>Immunoassays</topic><topic>Immunofluorescence</topic><topic>Inflammation</topic><topic>Interleukin 6</topic><topic>Interleukin 8</topic><topic>Interleukin-6 - genetics</topic><topic>Interleukin-6 - metabolism</topic><topic>Interleukin-8 - metabolism</topic><topic>Intracellular</topic><topic>Intracellular signalling</topic><topic>Iodides</topic><topic>Life Sciences</topic><topic>Localization</topic><topic>Lung - drug effects</topic><topic>Lung - metabolism</topic><topic>Lung - pathology</topic><topic>Lung diseases</topic><topic>Odorants</topic><topic>Pathogenesis</topic><topic>Perfumes</topic><topic>Pharmacology/Toxicology</topic><topic>Receptors</topic><topic>Receptors, Odorant - genetics</topic><topic>Receptors, Odorant - metabolism</topic><topic>Therapeutic targets</topic><topic>Type C Phospholipases - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Awad, Noha</creatorcontrib><creatorcontrib>Weidinger, Daniel</creatorcontrib><creatorcontrib>Greune, Lea</creatorcontrib><creatorcontrib>Kronsbein, Juliane</creatorcontrib><creatorcontrib>Heinen, Natalie</creatorcontrib><creatorcontrib>Westhoven, Saskia</creatorcontrib><creatorcontrib>Pfaender, Stephanie</creatorcontrib><creatorcontrib>Taube, Christian</creatorcontrib><creatorcontrib>Reuter, Sebastian</creatorcontrib><creatorcontrib>Peters, Marcus</creatorcontrib><creatorcontrib>Hatt, Hanns</creatorcontrib><creatorcontrib>Fender, Anke</creatorcontrib><creatorcontrib>Knobloch, Jürgen</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Premium</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Cell biology and toxicology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Awad, Noha</au><au>Weidinger, Daniel</au><au>Greune, Lea</au><au>Kronsbein, Juliane</au><au>Heinen, Natalie</au><au>Westhoven, Saskia</au><au>Pfaender, Stephanie</au><au>Taube, Christian</au><au>Reuter, Sebastian</au><au>Peters, Marcus</au><au>Hatt, Hanns</au><au>Fender, Anke</au><au>Knobloch, Jürgen</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Functional characterization of OR51B5 and OR1G1 in human lung epithelial cells as potential drug targets for non-type 2 lung diseases</atitle><jtitle>Cell biology and toxicology</jtitle><stitle>Cell Biol Toxicol</stitle><addtitle>Cell Biol Toxicol</addtitle><date>2024-11-13</date><risdate>2024</risdate><volume>40</volume><issue>1</issue><spage>96</spage><pages>96-</pages><artnum>96</artnum><issn>1573-6822</issn><issn>0742-2091</issn><eissn>1573-6822</eissn><abstract>Background
Hypersensitivity to odorants like perfumes can induce or promote asthma with non-type 2 inflammation for which therapeutic options are limited. Cell death of primary bronchial epithelial cells (PBECs) and the release of the pro-inflammatory cytokines interleukin-6 (IL-6) and IL-8 are key in the pathogenesis. Extra-nasal olfactory receptors (ORs) can influence cellular processes involved in asthma. This study investigated the utility of ORs in epithelial cells as potential drug targets in this context.
Methods
We used the A549 cell line and primary bronchial epithelial cells using air–liquid interface culture system (ALI-PBECs). OR expression was investigated by RT-PCR, Western blot, and Immunofluorescence. Effects of OR activation by specific ligands on intracellular calcium concentration, cAMP, Phospholipase C (PLC), cell viability, and IL-6 and IL-8 secretion were analyzed by calcium imaging, enzyme immunoassays, Annexin V/ propidium iodide -based fluorescence-activated cell staining or by ELISA, respectively.
Results
By screening A549 cells, the OR51B5 agonists Farnesol and Isononyl Alcohol and the OR1G1 agonist Nonanal increased intracellular Ca2 + . OR51B5 and OR1G1 mRNAs and proteins were detected. Both receptors showed a preferential intracellular localization. OR51B5- but not OR1G1-induced Ca2 + dependent on both cAMP and PLC signaling. Farnesol, Isononyl Alcohol, and Nonanal, all reduced cell viability and induced IL-8 and IL-6 release. The data were verified in ALI-PBECs.
Conclusion
ORs in the lung epithelium might be involved in airway-sensitivity to odorants. Their antagonism could represent a promising strategy in treatment of odorant-induced asthma with non-type 2 inflammation.
Graphical Abstract</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>39538061</pmid><doi>10.1007/s10565-024-09935-9</doi><oa>free_for_read</oa></addata></record> |
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| ispartof | Cell biology and toxicology, 2024-11, Vol.40 (1), p.96, Article 96 |
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| source | MEDLINE; SpringerLink Journals; Alma/SFX Local Collection |
| subjects | A549 Cells Agonists Annexin V Aroma compounds Asthma Biochemistry Biomedical and Life Sciences Calcium Calcium (intracellular) Calcium - metabolism Calcium imaging Cell Biology Cell culture Cell death Cell Survival - drug effects Cell viability Cyclic AMP Cyclic AMP - metabolism Enzyme-linked immunosorbent assay Epithelial cells Epithelial Cells - drug effects Epithelial Cells - metabolism Epithelium Farnesol Humans Hypersensitivity Immunoassays Immunofluorescence Inflammation Interleukin 6 Interleukin 8 Interleukin-6 - genetics Interleukin-6 - metabolism Interleukin-8 - metabolism Intracellular Intracellular signalling Iodides Life Sciences Localization Lung - drug effects Lung - metabolism Lung - pathology Lung diseases Odorants Pathogenesis Perfumes Pharmacology/Toxicology Receptors Receptors, Odorant - genetics Receptors, Odorant - metabolism Therapeutic targets Type C Phospholipases - metabolism |
| title | Functional characterization of OR51B5 and OR1G1 in human lung epithelial cells as potential drug targets for non-type 2 lung diseases |
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