Pulsed electric field at resonance frequency combat Klebsiella pneumonia biofilms
Healtcare-associated infections have increased due to the development of antimicrobial resistance (AMR) of Gram-negative pathogens (GNPs) and the development of outbreacks over the past two decades. In this work, we investigated how exposure to positive electric pulses affects the growth characteris...
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| Veröffentlicht in: | Applied microbiology and biotechnology 2024-12, Vol.108 (1), p.505, Article 505 |
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| creator | Mohamed, Dorria H. Mohammed, Haitham El-Gebaly, Reem H. Adam, Mohamed Ali, Fadel M. |
| description | Healtcare-associated infections have increased due to the development of antimicrobial resistance (AMR) of Gram-negative pathogens (GNPs) and the development of outbreacks over the past two decades. In this work, we investigated how exposure to positive electric pulses affects the growth characteristics of
Klebsiella pneumonia
(
K. pneumonia
), a common cause of pneumonia. We explored the impact of varying exposure frequencies (0.2–2 Hz) and time (15–90 min, at resonance frequency) on bioelectric signals produced during cell division, biofilm formation, and bacterial antibiotic susceptibility. Our research found that an extremely low-frequency pulsed electric field (ELF-PEF) significantly inhibited
K. pneumonia
growth. Specifically, exposure to 0.8 Hz for one hour increased the antibiotic susceptibility of
K. pneumonia
to inhibitors of cell wall formation, proteins, β-lactamase, DNA, and other substances. We also noticed a notable decrease in
K. pneumonia
biofilm development exposed to ELF-PEF. Our results suggest that the interaction of
K. pneumonia
cells with ELF-PEF at the specified frequency and time alters cellular activity and bacterial structure. This technique may be used in the future to treat
K. pneumonia
infections both in vitro and
in vivo.
Key points
•
Extremely low-frequency pulsed electric fields inhibit the growth of K. pneumonia.
•
ELF-PEF increases antibiotic susceptibility and reduces biofilm formation in K. pneumonia.
•
ELF-PEF showed potential for treating K. pneumonia infections. |
| doi_str_mv | 10.1007/s00253-024-13330-z |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_11538251</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>3124684167</sourcerecordid><originalsourceid>FETCH-LOGICAL-c356t-a3f2c9451ced5444663ba8094bacfa7605fd0f567fae2329303db51c4dae3a4b3</originalsourceid><addsrcrecordid>eNp9kc1OFjEUhhsjgQ_wBlyQSdy4GTn9nZmVMUTBSKIxuG46nVMomWk_2hkTuBqvxSuj8AGiC1ddnOc8p29eQl5TeEcBmsMMwCSvgYmacs6hvnlBVlRwVoOi4iVZAW1k3ciu3SG7OV8CUNYqtU12eCeLoBUr8v3bMmYcKhzRzsnbynkch8rMVcIcgwkWK5fwasFgrysbp76MvozY58KNploHXKYYvPn9q_fR-XHK-2TLmSJ99fDukR-fPp4dndSnX48_H304rS2Xaq4Nd8x2QlKLgxRCKMV700InemOdaRRIN4CTqnEGGWcdBz70hRaDQW5Ez_fI-413vfQTDhbDnMyo18lPJl3raLz-exL8hT6PPzWlkrdM0mJ4-2BIsSTMs558tne5AsYla06ZUK2gqinom3_Qy7ikUPLdU6xpWlCFYhvKpphzQvf0Gwr6rjO96UyXzvR9Z_qmLB08z_G08lhSAfgGyGUUzjH9uf0f7S2S1qTn</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>3124277806</pqid></control><display><type>article</type><title>Pulsed electric field at resonance frequency combat Klebsiella pneumonia biofilms</title><source>MEDLINE</source><source>Springer Nature OA Free Journals</source><source>Alma/SFX Local Collection</source><source>SpringerLink Journals - AutoHoldings</source><creator>Mohamed, Dorria H. ; Mohammed, Haitham ; El-Gebaly, Reem H. ; Adam, Mohamed ; Ali, Fadel M.</creator><creatorcontrib>Mohamed, Dorria H. ; Mohammed, Haitham ; El-Gebaly, Reem H. ; Adam, Mohamed ; Ali, Fadel M.</creatorcontrib><description>Healtcare-associated infections have increased due to the development of antimicrobial resistance (AMR) of Gram-negative pathogens (GNPs) and the development of outbreacks over the past two decades. In this work, we investigated how exposure to positive electric pulses affects the growth characteristics of
Klebsiella pneumonia
(
K. pneumonia
), a common cause of pneumonia. We explored the impact of varying exposure frequencies (0.2–2 Hz) and time (15–90 min, at resonance frequency) on bioelectric signals produced during cell division, biofilm formation, and bacterial antibiotic susceptibility. Our research found that an extremely low-frequency pulsed electric field (ELF-PEF) significantly inhibited
K. pneumonia
growth. Specifically, exposure to 0.8 Hz for one hour increased the antibiotic susceptibility of
K. pneumonia
to inhibitors of cell wall formation, proteins, β-lactamase, DNA, and other substances. We also noticed a notable decrease in
K. pneumonia
biofilm development exposed to ELF-PEF. Our results suggest that the interaction of
K. pneumonia
cells with ELF-PEF at the specified frequency and time alters cellular activity and bacterial structure. This technique may be used in the future to treat
K. pneumonia
infections both in vitro and
in vivo.
Key points
•
Extremely low-frequency pulsed electric fields inhibit the growth of K. pneumonia.
•
ELF-PEF increases antibiotic susceptibility and reduces biofilm formation in K. pneumonia.
•
ELF-PEF showed potential for treating K. pneumonia infections.</description><identifier>ISSN: 0175-7598</identifier><identifier>ISSN: 1432-0614</identifier><identifier>EISSN: 1432-0614</identifier><identifier>DOI: 10.1007/s00253-024-13330-z</identifier><identifier>PMID: 39500784</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Anti-Bacterial Agents - pharmacology ; Antibiotics ; Antimicrobial resistance ; Applied Microbial and Cell Physiology ; Bioelectricity ; Biofilms ; Biofilms - drug effects ; Biofilms - growth & development ; Biofilms - radiation effects ; Biomedical and Life Sciences ; Biotechnology ; Cell division ; Cell walls ; Cellular structure ; Electric fields ; Electric pulses ; Electricity ; Exposure ; In vivo methods and tests ; Infections ; Klebsiella ; Klebsiella Infections - microbiology ; Klebsiella pneumoniae ; Klebsiella pneumoniae - drug effects ; Klebsiella pneumoniae - physiology ; Life Sciences ; Low frequency ; Microbial Genetics and Genomics ; Microbial Sensitivity Tests ; Microbiology ; Pneumonia ; Resonance ; β Lactamase</subject><ispartof>Applied microbiology and biotechnology, 2024-12, Vol.108 (1), p.505, Article 505</ispartof><rights>The Author(s) 2024</rights><rights>2024. The Author(s).</rights><rights>The Author(s) 2024. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>The Author(s) 2024 2024</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c356t-a3f2c9451ced5444663ba8094bacfa7605fd0f567fae2329303db51c4dae3a4b3</cites><orcidid>0000-0002-6055-9514</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00253-024-13330-z$$EPDF$$P50$$Gspringer$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://doi.org/10.1007/s00253-024-13330-z$$EHTML$$P50$$Gspringer$$Hfree_for_read</linktohtml><link.rule.ids>230,314,780,784,885,27924,27925,41120,41488,42189,42557,51319,51576</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/39500784$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mohamed, Dorria H.</creatorcontrib><creatorcontrib>Mohammed, Haitham</creatorcontrib><creatorcontrib>El-Gebaly, Reem H.</creatorcontrib><creatorcontrib>Adam, Mohamed</creatorcontrib><creatorcontrib>Ali, Fadel M.</creatorcontrib><title>Pulsed electric field at resonance frequency combat Klebsiella pneumonia biofilms</title><title>Applied microbiology and biotechnology</title><addtitle>Appl Microbiol Biotechnol</addtitle><addtitle>Appl Microbiol Biotechnol</addtitle><description>Healtcare-associated infections have increased due to the development of antimicrobial resistance (AMR) of Gram-negative pathogens (GNPs) and the development of outbreacks over the past two decades. In this work, we investigated how exposure to positive electric pulses affects the growth characteristics of
Klebsiella pneumonia
(
K. pneumonia
), a common cause of pneumonia. We explored the impact of varying exposure frequencies (0.2–2 Hz) and time (15–90 min, at resonance frequency) on bioelectric signals produced during cell division, biofilm formation, and bacterial antibiotic susceptibility. Our research found that an extremely low-frequency pulsed electric field (ELF-PEF) significantly inhibited
K. pneumonia
growth. Specifically, exposure to 0.8 Hz for one hour increased the antibiotic susceptibility of
K. pneumonia
to inhibitors of cell wall formation, proteins, β-lactamase, DNA, and other substances. We also noticed a notable decrease in
K. pneumonia
biofilm development exposed to ELF-PEF. Our results suggest that the interaction of
K. pneumonia
cells with ELF-PEF at the specified frequency and time alters cellular activity and bacterial structure. This technique may be used in the future to treat
K. pneumonia
infections both in vitro and
in vivo.
Key points
•
Extremely low-frequency pulsed electric fields inhibit the growth of K. pneumonia.
•
ELF-PEF increases antibiotic susceptibility and reduces biofilm formation in K. pneumonia.
•
ELF-PEF showed potential for treating K. pneumonia infections.</description><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Antibiotics</subject><subject>Antimicrobial resistance</subject><subject>Applied Microbial and Cell Physiology</subject><subject>Bioelectricity</subject><subject>Biofilms</subject><subject>Biofilms - drug effects</subject><subject>Biofilms - growth & development</subject><subject>Biofilms - radiation effects</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>Cell division</subject><subject>Cell walls</subject><subject>Cellular structure</subject><subject>Electric fields</subject><subject>Electric pulses</subject><subject>Electricity</subject><subject>Exposure</subject><subject>In vivo methods and tests</subject><subject>Infections</subject><subject>Klebsiella</subject><subject>Klebsiella Infections - microbiology</subject><subject>Klebsiella pneumoniae</subject><subject>Klebsiella pneumoniae - drug effects</subject><subject>Klebsiella pneumoniae - physiology</subject><subject>Life Sciences</subject><subject>Low frequency</subject><subject>Microbial Genetics and Genomics</subject><subject>Microbial Sensitivity Tests</subject><subject>Microbiology</subject><subject>Pneumonia</subject><subject>Resonance</subject><subject>β Lactamase</subject><issn>0175-7598</issn><issn>1432-0614</issn><issn>1432-0614</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>EIF</sourceid><recordid>eNp9kc1OFjEUhhsjgQ_wBlyQSdy4GTn9nZmVMUTBSKIxuG46nVMomWk_2hkTuBqvxSuj8AGiC1ddnOc8p29eQl5TeEcBmsMMwCSvgYmacs6hvnlBVlRwVoOi4iVZAW1k3ciu3SG7OV8CUNYqtU12eCeLoBUr8v3bMmYcKhzRzsnbynkch8rMVcIcgwkWK5fwasFgrysbp76MvozY58KNploHXKYYvPn9q_fR-XHK-2TLmSJ99fDukR-fPp4dndSnX48_H304rS2Xaq4Nd8x2QlKLgxRCKMV700InemOdaRRIN4CTqnEGGWcdBz70hRaDQW5Ez_fI-413vfQTDhbDnMyo18lPJl3raLz-exL8hT6PPzWlkrdM0mJ4-2BIsSTMs558tne5AsYla06ZUK2gqinom3_Qy7ikUPLdU6xpWlCFYhvKpphzQvf0Gwr6rjO96UyXzvR9Z_qmLB08z_G08lhSAfgGyGUUzjH9uf0f7S2S1qTn</recordid><startdate>20241201</startdate><enddate>20241201</enddate><creator>Mohamed, Dorria H.</creator><creator>Mohammed, Haitham</creator><creator>El-Gebaly, Reem H.</creator><creator>Adam, Mohamed</creator><creator>Ali, Fadel M.</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>C6C</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-6055-9514</orcidid></search><sort><creationdate>20241201</creationdate><title>Pulsed electric field at resonance frequency combat Klebsiella pneumonia biofilms</title><author>Mohamed, Dorria H. ; Mohammed, Haitham ; El-Gebaly, Reem H. ; Adam, Mohamed ; Ali, Fadel M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c356t-a3f2c9451ced5444663ba8094bacfa7605fd0f567fae2329303db51c4dae3a4b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Antibiotics</topic><topic>Antimicrobial resistance</topic><topic>Applied Microbial and Cell Physiology</topic><topic>Bioelectricity</topic><topic>Biofilms</topic><topic>Biofilms - drug effects</topic><topic>Biofilms - growth & development</topic><topic>Biofilms - radiation effects</topic><topic>Biomedical and Life Sciences</topic><topic>Biotechnology</topic><topic>Cell division</topic><topic>Cell walls</topic><topic>Cellular structure</topic><topic>Electric fields</topic><topic>Electric pulses</topic><topic>Electricity</topic><topic>Exposure</topic><topic>In vivo methods and tests</topic><topic>Infections</topic><topic>Klebsiella</topic><topic>Klebsiella Infections - microbiology</topic><topic>Klebsiella pneumoniae</topic><topic>Klebsiella pneumoniae - drug effects</topic><topic>Klebsiella pneumoniae - physiology</topic><topic>Life Sciences</topic><topic>Low frequency</topic><topic>Microbial Genetics and Genomics</topic><topic>Microbial Sensitivity Tests</topic><topic>Microbiology</topic><topic>Pneumonia</topic><topic>Resonance</topic><topic>β Lactamase</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mohamed, Dorria H.</creatorcontrib><creatorcontrib>Mohammed, Haitham</creatorcontrib><creatorcontrib>El-Gebaly, Reem H.</creatorcontrib><creatorcontrib>Adam, Mohamed</creatorcontrib><creatorcontrib>Ali, Fadel M.</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Applied microbiology and biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mohamed, Dorria H.</au><au>Mohammed, Haitham</au><au>El-Gebaly, Reem H.</au><au>Adam, Mohamed</au><au>Ali, Fadel M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Pulsed electric field at resonance frequency combat Klebsiella pneumonia biofilms</atitle><jtitle>Applied microbiology and biotechnology</jtitle><stitle>Appl Microbiol Biotechnol</stitle><addtitle>Appl Microbiol Biotechnol</addtitle><date>2024-12-01</date><risdate>2024</risdate><volume>108</volume><issue>1</issue><spage>505</spage><pages>505-</pages><artnum>505</artnum><issn>0175-7598</issn><issn>1432-0614</issn><eissn>1432-0614</eissn><abstract>Healtcare-associated infections have increased due to the development of antimicrobial resistance (AMR) of Gram-negative pathogens (GNPs) and the development of outbreacks over the past two decades. In this work, we investigated how exposure to positive electric pulses affects the growth characteristics of
Klebsiella pneumonia
(
K. pneumonia
), a common cause of pneumonia. We explored the impact of varying exposure frequencies (0.2–2 Hz) and time (15–90 min, at resonance frequency) on bioelectric signals produced during cell division, biofilm formation, and bacterial antibiotic susceptibility. Our research found that an extremely low-frequency pulsed electric field (ELF-PEF) significantly inhibited
K. pneumonia
growth. Specifically, exposure to 0.8 Hz for one hour increased the antibiotic susceptibility of
K. pneumonia
to inhibitors of cell wall formation, proteins, β-lactamase, DNA, and other substances. We also noticed a notable decrease in
K. pneumonia
biofilm development exposed to ELF-PEF. Our results suggest that the interaction of
K. pneumonia
cells with ELF-PEF at the specified frequency and time alters cellular activity and bacterial structure. This technique may be used in the future to treat
K. pneumonia
infections both in vitro and
in vivo.
Key points
•
Extremely low-frequency pulsed electric fields inhibit the growth of K. pneumonia.
•
ELF-PEF increases antibiotic susceptibility and reduces biofilm formation in K. pneumonia.
•
ELF-PEF showed potential for treating K. pneumonia infections.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>39500784</pmid><doi>10.1007/s00253-024-13330-z</doi><orcidid>https://orcid.org/0000-0002-6055-9514</orcidid><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Springer Nature OA Free Journals; Alma/SFX Local Collection; SpringerLink Journals - AutoHoldings |
| subjects | Anti-Bacterial Agents - pharmacology Antibiotics Antimicrobial resistance Applied Microbial and Cell Physiology Bioelectricity Biofilms Biofilms - drug effects Biofilms - growth & development Biofilms - radiation effects Biomedical and Life Sciences Biotechnology Cell division Cell walls Cellular structure Electric fields Electric pulses Electricity Exposure In vivo methods and tests Infections Klebsiella Klebsiella Infections - microbiology Klebsiella pneumoniae Klebsiella pneumoniae - drug effects Klebsiella pneumoniae - physiology Life Sciences Low frequency Microbial Genetics and Genomics Microbial Sensitivity Tests Microbiology Pneumonia Resonance β Lactamase |
| title | Pulsed electric field at resonance frequency combat Klebsiella pneumonia biofilms |
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