Impact of Conjugated Linoleic Acid on Obesity and Its Association with Macrophage Recruitment: Experimental and Immunohistochemical Study
Conjugated linoleic acid (CLA) has been shown in humans and animals to have anti-adipose effects. The current study aims to assess the prophylactic and therapeutic impact of CLA and its effect on recruited macrophage type using immunohistochemistry against CD68 and CD 163. Forty adult male albino ra...
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| Veröffentlicht in: | The journal of microscopy & ultrastructure 2024-07, Vol.12 (3), p.142-147 |
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| creator | Abdou, Asmaa Gaber Bendary, Mohamed AbdElfattah Abdou, Sara E Amer, Ghada S |
| description | Conjugated linoleic acid (CLA) has been shown in humans and animals to have anti-adipose effects. The current study aims to assess the prophylactic and therapeutic impact of CLA and its effect on recruited macrophage type using immunohistochemistry against CD68 and CD 163.
Forty adult male albino rats of local strain were included in the study and divided into control, CLA-supplemented, obese, CLA-prophylactic obese, and CLA-treated obese groups. Biopsies from visceral fat of the investigated groups were obtained and assessed for histopathological changes and immunohistochemical staining for CD68 and CD163.
Obese group showed hypertrophied adipocytes and infiltration by inflammatory cells compared to other groups. The obese group showed a marked increase in the CD68 positivity compared with that in the control and CLA-supplemented groups. CLA-prophylactic and CLA-treated groups showed mild immune reaction with a significant decrease in CD68 positivity compared to the obese group. The obese group showed a significant decrease in the CD163 positivity compared with that in the control and CLA-supplemented groups.
Adipose tissue in obese is characterized by inflammation with more M1 than M2 macrophages. CLA could direct the recruited macrophages toward the anti-inflammatory subtype (M2) which encourages its beneficial effects in prophylaxis from obesity. |
| doi_str_mv | 10.4103/jmau.jmau_25_22 |
| format | Article |
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Forty adult male albino rats of local strain were included in the study and divided into control, CLA-supplemented, obese, CLA-prophylactic obese, and CLA-treated obese groups. Biopsies from visceral fat of the investigated groups were obtained and assessed for histopathological changes and immunohistochemical staining for CD68 and CD163.
Obese group showed hypertrophied adipocytes and infiltration by inflammatory cells compared to other groups. The obese group showed a marked increase in the CD68 positivity compared with that in the control and CLA-supplemented groups. CLA-prophylactic and CLA-treated groups showed mild immune reaction with a significant decrease in CD68 positivity compared to the obese group. The obese group showed a significant decrease in the CD163 positivity compared with that in the control and CLA-supplemented groups.
Adipose tissue in obese is characterized by inflammation with more M1 than M2 macrophages. CLA could direct the recruited macrophages toward the anti-inflammatory subtype (M2) which encourages its beneficial effects in prophylaxis from obesity.</description><identifier>ISSN: 2213-879X</identifier><identifier>ISSN: 2213-8803</identifier><identifier>EISSN: 2213-8803</identifier><identifier>DOI: 10.4103/jmau.jmau_25_22</identifier><identifier>PMID: 39507643</identifier><language>eng</language><publisher>India: Medknow Publications and Media Pvt. Ltd</publisher><subject>Health aspects ; Immunohistochemistry ; Linoleic acids ; Macrophages ; Original ; Type 2 diabetes ; Unsaturated fatty acids</subject><ispartof>The journal of microscopy & ultrastructure, 2024-07, Vol.12 (3), p.142-147</ispartof><rights>Copyright: © 2022 Journal of Microscopy and Ultrastructure.</rights><rights>COPYRIGHT 2024 Medknow Publications and Media Pvt. Ltd.</rights><rights>Copyright: © 2022 Journal of Microscopy and Ultrastructure 2022</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c2190-c261ba13298f0b88795680c78374c4ee8e15b6b83644d8e383db930c527fc673</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC11537360/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC11537360/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/39507643$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Abdou, Asmaa Gaber</creatorcontrib><creatorcontrib>Bendary, Mohamed AbdElfattah</creatorcontrib><creatorcontrib>Abdou, Sara E</creatorcontrib><creatorcontrib>Amer, Ghada S</creatorcontrib><title>Impact of Conjugated Linoleic Acid on Obesity and Its Association with Macrophage Recruitment: Experimental and Immunohistochemical Study</title><title>The journal of microscopy & ultrastructure</title><addtitle>J Microsc Ultrastruct</addtitle><description>Conjugated linoleic acid (CLA) has been shown in humans and animals to have anti-adipose effects. The current study aims to assess the prophylactic and therapeutic impact of CLA and its effect on recruited macrophage type using immunohistochemistry against CD68 and CD 163.
Forty adult male albino rats of local strain were included in the study and divided into control, CLA-supplemented, obese, CLA-prophylactic obese, and CLA-treated obese groups. Biopsies from visceral fat of the investigated groups were obtained and assessed for histopathological changes and immunohistochemical staining for CD68 and CD163.
Obese group showed hypertrophied adipocytes and infiltration by inflammatory cells compared to other groups. The obese group showed a marked increase in the CD68 positivity compared with that in the control and CLA-supplemented groups. CLA-prophylactic and CLA-treated groups showed mild immune reaction with a significant decrease in CD68 positivity compared to the obese group. The obese group showed a significant decrease in the CD163 positivity compared with that in the control and CLA-supplemented groups.
Adipose tissue in obese is characterized by inflammation with more M1 than M2 macrophages. CLA could direct the recruited macrophages toward the anti-inflammatory subtype (M2) which encourages its beneficial effects in prophylaxis from obesity.</description><subject>Health aspects</subject><subject>Immunohistochemistry</subject><subject>Linoleic acids</subject><subject>Macrophages</subject><subject>Original</subject><subject>Type 2 diabetes</subject><subject>Unsaturated fatty acids</subject><issn>2213-879X</issn><issn>2213-8803</issn><issn>2213-8803</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNptUk1r3DAQNaWlCWnOvRVBL73sRp-23EtZlqRd2BJIc-hNyPJ4rcWWXEtusz-h_7pyN0kbCILRMHrvMTN6WfaW4CUnmF3sez0t56CoUJS-yE4pJWwhJWYvH_Ki_H6SnYewxxgTSfOck9fZCSsFLnLOTrPfm37QJiLfoLV3-2mnI9Roa53vwBq0MrZG3qHrCoKNB6RdjTYxoFUI3lgdbXr7ZWOLvmoz-qHVO0A3YMbJxh5c_Igu7wYY7Zzr7sju-8n51oboTQu9Nan-LU714U32qtFdgPP7-yy7vbq8XX9ZbK8_b9ar7cJQUuIUc1JpwmgpG1zJNKDIJTaFZAU3HEACEVVeSZZzXktgktVVybARtGhMXrCz7NNRdpiqHmqTOht1p4bUpB4Pymurnr4426qd_6kIEaxgOU4KH-4VRv9jghBVb4OBrtMO_BQUI1Twkhdihr4_Qne6A2Vd45OkmeFqJYkQ6aeKMqGWz6DSqef9eAeNTfUnhIsjIe08hBGax_YJVrM11F9X_LNGYrz7f-pH_IMR2B_Z6rg6</recordid><startdate>202407</startdate><enddate>202407</enddate><creator>Abdou, Asmaa Gaber</creator><creator>Bendary, Mohamed AbdElfattah</creator><creator>Abdou, Sara E</creator><creator>Amer, Ghada S</creator><general>Medknow Publications and Media Pvt. Ltd</general><general>Wolters Kluwer - Medknow</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>202407</creationdate><title>Impact of Conjugated Linoleic Acid on Obesity and Its Association with Macrophage Recruitment: Experimental and Immunohistochemical Study</title><author>Abdou, Asmaa Gaber ; Bendary, Mohamed AbdElfattah ; Abdou, Sara E ; Amer, Ghada S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2190-c261ba13298f0b88795680c78374c4ee8e15b6b83644d8e383db930c527fc673</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Health aspects</topic><topic>Immunohistochemistry</topic><topic>Linoleic acids</topic><topic>Macrophages</topic><topic>Original</topic><topic>Type 2 diabetes</topic><topic>Unsaturated fatty acids</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Abdou, Asmaa Gaber</creatorcontrib><creatorcontrib>Bendary, Mohamed AbdElfattah</creatorcontrib><creatorcontrib>Abdou, Sara E</creatorcontrib><creatorcontrib>Amer, Ghada S</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The journal of microscopy & ultrastructure</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Abdou, Asmaa Gaber</au><au>Bendary, Mohamed AbdElfattah</au><au>Abdou, Sara E</au><au>Amer, Ghada S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Impact of Conjugated Linoleic Acid on Obesity and Its Association with Macrophage Recruitment: Experimental and Immunohistochemical Study</atitle><jtitle>The journal of microscopy & ultrastructure</jtitle><addtitle>J Microsc Ultrastruct</addtitle><date>2024-07</date><risdate>2024</risdate><volume>12</volume><issue>3</issue><spage>142</spage><epage>147</epage><pages>142-147</pages><issn>2213-879X</issn><issn>2213-8803</issn><eissn>2213-8803</eissn><abstract>Conjugated linoleic acid (CLA) has been shown in humans and animals to have anti-adipose effects. The current study aims to assess the prophylactic and therapeutic impact of CLA and its effect on recruited macrophage type using immunohistochemistry against CD68 and CD 163.
Forty adult male albino rats of local strain were included in the study and divided into control, CLA-supplemented, obese, CLA-prophylactic obese, and CLA-treated obese groups. Biopsies from visceral fat of the investigated groups were obtained and assessed for histopathological changes and immunohistochemical staining for CD68 and CD163.
Obese group showed hypertrophied adipocytes and infiltration by inflammatory cells compared to other groups. The obese group showed a marked increase in the CD68 positivity compared with that in the control and CLA-supplemented groups. CLA-prophylactic and CLA-treated groups showed mild immune reaction with a significant decrease in CD68 positivity compared to the obese group. The obese group showed a significant decrease in the CD163 positivity compared with that in the control and CLA-supplemented groups.
Adipose tissue in obese is characterized by inflammation with more M1 than M2 macrophages. CLA could direct the recruited macrophages toward the anti-inflammatory subtype (M2) which encourages its beneficial effects in prophylaxis from obesity.</abstract><cop>India</cop><pub>Medknow Publications and Media Pvt. Ltd</pub><pmid>39507643</pmid><doi>10.4103/jmau.jmau_25_22</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Health aspects Immunohistochemistry Linoleic acids Macrophages Original Type 2 diabetes Unsaturated fatty acids |
| title | Impact of Conjugated Linoleic Acid on Obesity and Its Association with Macrophage Recruitment: Experimental and Immunohistochemical Study |
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