Characterization of Spatial Differences in Two Misfolded Proteins During Aggresome Formation

Cells have evolved an elaborate network of folding and degradation pathways to maintain the native state of proteins. If these pathways are disrupted (e.g., mutation) or their capacity is exceeded then protein aggregates form in cells. Cells sequester these aggregated proteins into aggresome or aggr...

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Veröffentlicht in:	microPublication biology 2024, Vol.2024
Hauptverfasser:	Goldy, Jordan N, Youker, Robert T
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Sprache:	eng
Schlagworte:	Cell Culture Human New Finding
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container_title	microPublication biology
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creator	Goldy, Jordan N Youker, Robert T
description	Cells have evolved an elaborate network of folding and degradation pathways to maintain the native state of proteins. If these pathways are disrupted (e.g., mutation) or their capacity is exceeded then protein aggregates form in cells. Cells sequester these aggregated proteins into aggresome or aggresome-like bodies as a protective mechanism. In this study, we co-expressed two model misfolded proteins in HEK293 cells and measured aggresome formation upon proteasomal inhibition. We observed spatial differences in early time points of aggresome formation upon co-expression of the misfolded proteins compared to individual expression in cells.
doi_str_mv	10.17912/micropub.biology.001312
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title	Characterization of Spatial Differences in Two Misfolded Proteins During Aggresome Formation
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