The protective effects of antioxidants against endogenous and exogenous oxidative stress on bull sperm
Oxidative stress, caused by both endogenous and exogenous factors, affects sperm function by damaging morphology and reducing metabolic activity, leading to reduced fertilization ability. The purpose of this study was to investigate the effects of oxidative stress on bull sperm and to evaluate the e...
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| Veröffentlicht in: | In vitro cellular & developmental biology. Animal 2024-10, Vol.60 (9), p.969-982 |
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| creator | Younus, Ali MD Yamanaka, Takahiro Shimada, Masayuki |
| description | Oxidative stress, caused by both endogenous and exogenous factors, affects sperm function by damaging morphology and reducing metabolic activity, leading to reduced fertilization ability. The purpose of this study was to investigate the effects of oxidative stress on bull sperm and to evaluate the efficacy of targeted antioxidants in mitigating these detrimental effects. Fresh bull semen samples were subjected to hydrogen peroxide (H
2
O
2
) and antimycin treatments to induce oxidative stress, and the antioxidants PQQ, ergothioneine, and vitamin C were applied to counteract the induced stress. Sperm motility, viability, and reactive oxygen species (ROS) levels in the cytoplasm and mitochondria of sperm were assessed using computer-assisted sperm analysis (CASA) and flow cytometry. The treatment with H
2
O
2
rapidly decreased sperm viability, and antimycin-induced mitochondrial ROS mainly decreased sperm motility; PQQ and vitamin C effectively reduced mitochondrial ROS, while ergothioneine and vitamin C reduced cytosolic ROS. In frozen-thawed sperm, oxidative stress was elevated in both cytoplasm and mitochondria, and all three antioxidants improved sperm motility by inhibiting ROS production. Furthermore, the localization of oxidized lipids (4-hydroxynonenal) in sperm was detected using immunofluorescence, indicating that oxidative stress affects the head and midpiece of sperm. These findings highlight the potential of targeted antioxidants to mitigate the detrimental effects of oxidative stress on bull sperm and provide valuable insights to improve semen quality and optimize the use of antioxidants in artificial insemination. |
| doi_str_mv | 10.1007/s11626-024-00944-w |
| format | Article |
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2
O
2
) and antimycin treatments to induce oxidative stress, and the antioxidants PQQ, ergothioneine, and vitamin C were applied to counteract the induced stress. Sperm motility, viability, and reactive oxygen species (ROS) levels in the cytoplasm and mitochondria of sperm were assessed using computer-assisted sperm analysis (CASA) and flow cytometry. The treatment with H
2
O
2
rapidly decreased sperm viability, and antimycin-induced mitochondrial ROS mainly decreased sperm motility; PQQ and vitamin C effectively reduced mitochondrial ROS, while ergothioneine and vitamin C reduced cytosolic ROS. In frozen-thawed sperm, oxidative stress was elevated in both cytoplasm and mitochondria, and all three antioxidants improved sperm motility by inhibiting ROS production. Furthermore, the localization of oxidized lipids (4-hydroxynonenal) in sperm was detected using immunofluorescence, indicating that oxidative stress affects the head and midpiece of sperm. These findings highlight the potential of targeted antioxidants to mitigate the detrimental effects of oxidative stress on bull sperm and provide valuable insights to improve semen quality and optimize the use of antioxidants in artificial insemination.</description><identifier>ISSN: 1071-2690</identifier><identifier>ISSN: 1543-706X</identifier><identifier>EISSN: 1543-706X</identifier><identifier>DOI: 10.1007/s11626-024-00944-w</identifier><identifier>PMID: 39080183</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>4-Hydroxynonenal ; Animal Genetics and Genomics ; Animals ; Antioxidants ; Antioxidants - pharmacology ; Artificial insemination ; Ascorbic acid ; biochemical pathways ; Biomedical and Life Sciences ; Cattle ; Cell Biology ; Cell Culture ; Cell Survival - drug effects ; Cryopreservation - methods ; Cytoplasm ; Damage assessment ; Developmental Biology ; Fertilization ; Flow cytometry ; fluorescent antibody technique ; freeze-thaw cycles ; head ; Hydrogen peroxide ; Hydrogen Peroxide - pharmacology ; Immunofluorescence ; Life Sciences ; Lipids ; Localization ; Male ; Mitochondria ; Mitochondria - drug effects ; Mitochondria - metabolism ; Motility ; oxidation ; Oxidative stress ; Oxidative Stress - drug effects ; Reactive oxygen species ; Reactive Oxygen Species - metabolism ; Semen ; Semen Analysis ; Semen Preservation - methods ; semen quality ; Sperm ; sperm motility ; Sperm Motility - drug effects ; spermatozoa ; Spermatozoa - drug effects ; Spermatozoa - metabolism ; Stem Cells ; viability ; Vitamin C</subject><ispartof>In vitro cellular & developmental biology. Animal, 2024-10, Vol.60 (9), p.969-982</ispartof><rights>The Author(s) 2024</rights><rights>2024. The Author(s).</rights><rights>The Author(s) 2024. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>The Author(s) 2024 2024</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c389t-4807940b6d9a448f84391c5d6421d22605a7fe8abd2cb4176846f2197c2e6cd83</cites><orcidid>0000-0001-9234-4289 ; 0000-0002-6500-2088</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11626-024-00944-w$$EPDF$$P50$$Gspringer$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11626-024-00944-w$$EHTML$$P50$$Gspringer$$Hfree_for_read</linktohtml><link.rule.ids>230,314,776,780,881,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/39080183$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Younus, Ali MD</creatorcontrib><creatorcontrib>Yamanaka, Takahiro</creatorcontrib><creatorcontrib>Shimada, Masayuki</creatorcontrib><title>The protective effects of antioxidants against endogenous and exogenous oxidative stress on bull sperm</title><title>In vitro cellular & developmental biology. Animal</title><addtitle>In Vitro Cell.Dev.Biol.-Animal</addtitle><addtitle>In Vitro Cell Dev Biol Anim</addtitle><description>Oxidative stress, caused by both endogenous and exogenous factors, affects sperm function by damaging morphology and reducing metabolic activity, leading to reduced fertilization ability. The purpose of this study was to investigate the effects of oxidative stress on bull sperm and to evaluate the efficacy of targeted antioxidants in mitigating these detrimental effects. Fresh bull semen samples were subjected to hydrogen peroxide (H
2
O
2
) and antimycin treatments to induce oxidative stress, and the antioxidants PQQ, ergothioneine, and vitamin C were applied to counteract the induced stress. Sperm motility, viability, and reactive oxygen species (ROS) levels in the cytoplasm and mitochondria of sperm were assessed using computer-assisted sperm analysis (CASA) and flow cytometry. The treatment with H
2
O
2
rapidly decreased sperm viability, and antimycin-induced mitochondrial ROS mainly decreased sperm motility; PQQ and vitamin C effectively reduced mitochondrial ROS, while ergothioneine and vitamin C reduced cytosolic ROS. In frozen-thawed sperm, oxidative stress was elevated in both cytoplasm and mitochondria, and all three antioxidants improved sperm motility by inhibiting ROS production. Furthermore, the localization of oxidized lipids (4-hydroxynonenal) in sperm was detected using immunofluorescence, indicating that oxidative stress affects the head and midpiece of sperm. These findings highlight the potential of targeted antioxidants to mitigate the detrimental effects of oxidative stress on bull sperm and provide valuable insights to improve semen quality and optimize the use of antioxidants in artificial insemination.</description><subject>4-Hydroxynonenal</subject><subject>Animal Genetics and Genomics</subject><subject>Animals</subject><subject>Antioxidants</subject><subject>Antioxidants - pharmacology</subject><subject>Artificial insemination</subject><subject>Ascorbic acid</subject><subject>biochemical pathways</subject><subject>Biomedical and Life Sciences</subject><subject>Cattle</subject><subject>Cell Biology</subject><subject>Cell Culture</subject><subject>Cell Survival - drug effects</subject><subject>Cryopreservation - methods</subject><subject>Cytoplasm</subject><subject>Damage assessment</subject><subject>Developmental Biology</subject><subject>Fertilization</subject><subject>Flow cytometry</subject><subject>fluorescent antibody technique</subject><subject>freeze-thaw cycles</subject><subject>head</subject><subject>Hydrogen peroxide</subject><subject>Hydrogen Peroxide - pharmacology</subject><subject>Immunofluorescence</subject><subject>Life Sciences</subject><subject>Lipids</subject><subject>Localization</subject><subject>Male</subject><subject>Mitochondria</subject><subject>Mitochondria - drug effects</subject><subject>Mitochondria - metabolism</subject><subject>Motility</subject><subject>oxidation</subject><subject>Oxidative stress</subject><subject>Oxidative Stress - drug effects</subject><subject>Reactive oxygen species</subject><subject>Reactive Oxygen Species - metabolism</subject><subject>Semen</subject><subject>Semen Analysis</subject><subject>Semen Preservation - methods</subject><subject>semen quality</subject><subject>Sperm</subject><subject>sperm motility</subject><subject>Sperm Motility - drug effects</subject><subject>spermatozoa</subject><subject>Spermatozoa - drug effects</subject><subject>Spermatozoa - metabolism</subject><subject>Stem Cells</subject><subject>viability</subject><subject>Vitamin C</subject><issn>1071-2690</issn><issn>1543-706X</issn><issn>1543-706X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>EIF</sourceid><recordid>eNqFkU1v1DAQhiMEoqXwBzigSFy4BMYf8ccJoYoWpEpcWqk3y7HH21RZe7GTtvx73N22lB7Al7HHz7z2zNs0bwl8JADyUyFEUNEB5R2A5ry7ftbsk56zToI4f173IElHhYa95lUpl1CXJuJls8c0KCCK7Tfh9ALbTU4zunm8whZDqLvSptDaOI_pZvQ1ltau7BjL3GL0aYUxLTUVfYs396ctuZUoc8ZSE7Edlmlqywbz-nXzItip4Ju7eNCcHX09PfzWnfw4_n745aRzTOm54wqk5jAIry3nKijONHG9F5wST6mA3sqAyg6euoETKRQXgRItHUXhvGIHzeed7mYZ1ugdxjnbyWzyuLb5l0l2NH_fxPHCrNKVIaRnXEtWFT7cKeT0c8Eym_VYHE6TjVj7NKwOmEhgivwfBSWYAk55Rd8_QS_TkmMdRRWkvNrZy1uK7iiXUykZw8PHCZhby83OclMtN1vLzXUteve45YeSe48rwHZAqVdxhfnP2_-Q_Q23q7i9</recordid><startdate>20241001</startdate><enddate>20241001</enddate><creator>Younus, Ali MD</creator><creator>Yamanaka, Takahiro</creator><creator>Shimada, Masayuki</creator><general>Springer US</general><general>Society for In Vitro Biology</general><scope>C6C</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>4T-</scope><scope>7QL</scope><scope>7T7</scope><scope>7TK</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0001-9234-4289</orcidid><orcidid>https://orcid.org/0000-0002-6500-2088</orcidid></search><sort><creationdate>20241001</creationdate><title>The protective effects of antioxidants against endogenous and exogenous oxidative stress on bull sperm</title><author>Younus, Ali MD ; Yamanaka, Takahiro ; Shimada, Masayuki</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c389t-4807940b6d9a448f84391c5d6421d22605a7fe8abd2cb4176846f2197c2e6cd83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>4-Hydroxynonenal</topic><topic>Animal Genetics and Genomics</topic><topic>Animals</topic><topic>Antioxidants</topic><topic>Antioxidants - pharmacology</topic><topic>Artificial insemination</topic><topic>Ascorbic acid</topic><topic>biochemical pathways</topic><topic>Biomedical and Life Sciences</topic><topic>Cattle</topic><topic>Cell Biology</topic><topic>Cell Culture</topic><topic>Cell Survival - drug effects</topic><topic>Cryopreservation - methods</topic><topic>Cytoplasm</topic><topic>Damage assessment</topic><topic>Developmental Biology</topic><topic>Fertilization</topic><topic>Flow cytometry</topic><topic>fluorescent antibody technique</topic><topic>freeze-thaw cycles</topic><topic>head</topic><topic>Hydrogen peroxide</topic><topic>Hydrogen Peroxide - pharmacology</topic><topic>Immunofluorescence</topic><topic>Life Sciences</topic><topic>Lipids</topic><topic>Localization</topic><topic>Male</topic><topic>Mitochondria</topic><topic>Mitochondria - drug effects</topic><topic>Mitochondria - metabolism</topic><topic>Motility</topic><topic>oxidation</topic><topic>Oxidative stress</topic><topic>Oxidative Stress - drug effects</topic><topic>Reactive oxygen species</topic><topic>Reactive Oxygen Species - metabolism</topic><topic>Semen</topic><topic>Semen Analysis</topic><topic>Semen Preservation - methods</topic><topic>semen quality</topic><topic>Sperm</topic><topic>sperm motility</topic><topic>Sperm Motility - drug effects</topic><topic>spermatozoa</topic><topic>Spermatozoa - drug effects</topic><topic>Spermatozoa - metabolism</topic><topic>Stem Cells</topic><topic>viability</topic><topic>Vitamin C</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Younus, Ali MD</creatorcontrib><creatorcontrib>Yamanaka, Takahiro</creatorcontrib><creatorcontrib>Shimada, Masayuki</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Docstoc</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>In vitro cellular & developmental biology. Animal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Younus, Ali MD</au><au>Yamanaka, Takahiro</au><au>Shimada, Masayuki</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The protective effects of antioxidants against endogenous and exogenous oxidative stress on bull sperm</atitle><jtitle>In vitro cellular & developmental biology. Animal</jtitle><stitle>In Vitro Cell.Dev.Biol.-Animal</stitle><addtitle>In Vitro Cell Dev Biol Anim</addtitle><date>2024-10-01</date><risdate>2024</risdate><volume>60</volume><issue>9</issue><spage>969</spage><epage>982</epage><pages>969-982</pages><issn>1071-2690</issn><issn>1543-706X</issn><eissn>1543-706X</eissn><abstract>Oxidative stress, caused by both endogenous and exogenous factors, affects sperm function by damaging morphology and reducing metabolic activity, leading to reduced fertilization ability. The purpose of this study was to investigate the effects of oxidative stress on bull sperm and to evaluate the efficacy of targeted antioxidants in mitigating these detrimental effects. Fresh bull semen samples were subjected to hydrogen peroxide (H
2
O
2
) and antimycin treatments to induce oxidative stress, and the antioxidants PQQ, ergothioneine, and vitamin C were applied to counteract the induced stress. Sperm motility, viability, and reactive oxygen species (ROS) levels in the cytoplasm and mitochondria of sperm were assessed using computer-assisted sperm analysis (CASA) and flow cytometry. The treatment with H
2
O
2
rapidly decreased sperm viability, and antimycin-induced mitochondrial ROS mainly decreased sperm motility; PQQ and vitamin C effectively reduced mitochondrial ROS, while ergothioneine and vitamin C reduced cytosolic ROS. In frozen-thawed sperm, oxidative stress was elevated in both cytoplasm and mitochondria, and all three antioxidants improved sperm motility by inhibiting ROS production. Furthermore, the localization of oxidized lipids (4-hydroxynonenal) in sperm was detected using immunofluorescence, indicating that oxidative stress affects the head and midpiece of sperm. These findings highlight the potential of targeted antioxidants to mitigate the detrimental effects of oxidative stress on bull sperm and provide valuable insights to improve semen quality and optimize the use of antioxidants in artificial insemination.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>39080183</pmid><doi>10.1007/s11626-024-00944-w</doi><tpages>14</tpages><orcidid>https://orcid.org/0000-0001-9234-4289</orcidid><orcidid>https://orcid.org/0000-0002-6500-2088</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | In vitro cellular & developmental biology. Animal, 2024-10, Vol.60 (9), p.969-982 |
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| subjects | 4-Hydroxynonenal Animal Genetics and Genomics Animals Antioxidants Antioxidants - pharmacology Artificial insemination Ascorbic acid biochemical pathways Biomedical and Life Sciences Cattle Cell Biology Cell Culture Cell Survival - drug effects Cryopreservation - methods Cytoplasm Damage assessment Developmental Biology Fertilization Flow cytometry fluorescent antibody technique freeze-thaw cycles head Hydrogen peroxide Hydrogen Peroxide - pharmacology Immunofluorescence Life Sciences Lipids Localization Male Mitochondria Mitochondria - drug effects Mitochondria - metabolism Motility oxidation Oxidative stress Oxidative Stress - drug effects Reactive oxygen species Reactive Oxygen Species - metabolism Semen Semen Analysis Semen Preservation - methods semen quality Sperm sperm motility Sperm Motility - drug effects spermatozoa Spermatozoa - drug effects Spermatozoa - metabolism Stem Cells viability Vitamin C |
| title | The protective effects of antioxidants against endogenous and exogenous oxidative stress on bull sperm |
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