Use of digitonin extraction to distinguish between autophagic-lysosomal sequestration and mitochondrial uptake of [14C]sucrose in hepatocytes

In isolated rat hepatocytes, electroinjected [14C]sucrose is sequestered both by mitochondria and by autophagosomes/lysosomes. Radioactivity can be selectively extracted from the latter organelles by low concentrations of digitonin, thereby providing a specific bioassay for autophagic sequestration....

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Veröffentlicht in:	Biochemical journal 1985-12, Vol.232 (3), p.773-780
Hauptverfasser:	Gordon, P B, Tolleshaug, H, Seglen, P O
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenine - analogs & derivatives Adenine - pharmacology Amino Acids - pharmacology Animals Autophagy - drug effects Digitonin - pharmacology In Vitro Techniques Liver - cytology Liver - drug effects Liver - metabolism Lysosomes - drug effects Lysosomes - metabolism Male Mitochondria, Liver - metabolism Phagocytosis - drug effects Rats Rats, Inbred Strains Sucrose - metabolism
Online-Zugang:	Volltext
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