Importance of sites of tracer administration and blood sampling in relation to leucine metabolism. Practical considerations
For the same infusion site of L-[1-14C]leucine, sampling downstream of arterial blood underestimates leucine turnover, whereas sampling of venous blood overestimates turnover. Further, the lungs release a small but consistent amount of leucine into the blood. Unlabelled leucine also is produced by t...
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| Veröffentlicht in: | Biochemical journal 1983-09, Vol.214 (3), p.1015-1018 |
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| container_title | Biochemical journal |
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| creator | Pell, J M Caldarone, E M Bergman, E N |
| description | For the same infusion site of L-[1-14C]leucine, sampling downstream of arterial blood underestimates leucine turnover, whereas sampling of venous blood overestimates turnover. Further, the lungs release a small but consistent amount of leucine into the blood. Unlabelled leucine also is produced by the portal-drained viscera, and some is removed immediately by the liver. These sources of leucine should thus be considered in turnover calculations. |
| doi_str_mv | 10.1042/bj2141015 |
| format | Article |
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These sources of leucine should thus be considered in turnover calculations.</description><subject>Animals</subject><subject>Arteries</subject><subject>Blood Specimen Collection</subject><subject>Carbon Radioisotopes</subject><subject>Leucine - blood</subject><subject>Leucine - metabolism</subject><subject>Liver - metabolism</subject><subject>Lung - metabolism</subject><subject>Sheep</subject><subject>Veins</subject><issn>0264-6021</issn><issn>1470-8728</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1983</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkU1LAzEQhoMotX4c_AFCToKHrUk2m00vgohfIOhBzyGbzNZINqlJKoh_3pWWoqeZYV6e-XgROqFkRglnF907o5wS2uygKeUtqWTL5C6aEiZ4JQij--gg53dCKCecTNBECCZow6bo-2FYxlR0MIBjj7MrkH-TkrSBhLUdXHB5rIqLAetgcedjtDjrYeldWGAXcAK_bpeIPayMC4AHKLqL3uVhhp9HVnFGe2xiyM7CmpaP0F6vfYbjTTxEr7c3L9f31ePT3cP11WNl6paUqm_q2nIjaN8Y27SSzhth55JRybnoodO6l6alUmhJeDe-QUAtBHTGGiEkqetDdLnmLlfdANZAGO_xapncoNOXitqp_53g3tQifio6vqjmcgScbQApfqwgFzW4bMB7HSCuspJEzBkV7Sg8XwtNijkn6LdDKFG_TqmtU6P29O9WW-XGmvoHekeRlA</recordid><startdate>19830915</startdate><enddate>19830915</enddate><creator>Pell, J M</creator><creator>Caldarone, E M</creator><creator>Bergman, E N</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19830915</creationdate><title>Importance of sites of tracer administration and blood sampling in relation to leucine metabolism. 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| ispartof | Biochemical journal, 1983-09, Vol.214 (3), p.1015-1018 |
| issn | 0264-6021 1470-8728 |
| language | eng |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; PubMed Central; Alma/SFX Local Collection |
| subjects | Animals Arteries Blood Specimen Collection Carbon Radioisotopes Leucine - blood Leucine - metabolism Liver - metabolism Lung - metabolism Sheep Veins |
| title | Importance of sites of tracer administration and blood sampling in relation to leucine metabolism. Practical considerations |
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