Towards integrating vectors for gene therapy: expression of functional bacteriophage MuA and MuB proteins in mammalian cells

Bacteriophage Mu has one of the best studied, most efficient and largest transposition machineries of the prokaryotic world. To harness this attractive integration machinery for use in mammalian cells, we cloned the coding sequences of the phage factors MuA and MuB in a eukaryotic expression cassett...

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Veröffentlicht in:	Nucleic acids research 2000-12, Vol.28 (23), p.E104-104
Hauptverfasser:	Schagen, F H, Rademaker, H J, Cramer, S J, van Ormondt, H, van der Eb, A J, van de Putte, P, Hoeben, R C
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacteriophage mu - genetics Cell Line, Transformed DNA Transposable Elements - genetics DNA, Recombinant DNA-Binding Proteins - genetics Escherichia coli Escherichia coli - genetics Escherichia coli - virology Gene Expression Gene Transfer Techniques Genetic Complementation Test Genetic Therapy - methods Genetic Vectors - genetics Green Fluorescent Proteins Humans hygromycin Luminescent Proteins - genetics Luminescent Proteins - metabolism MuA protein MuB protein NAR Methods Online Oligopeptides Peptides - genetics Phage Mu Plasmids - genetics Recombinant Fusion Proteins - genetics Recombinant Fusion Proteins - metabolism Transfection Transposases - genetics Tumor Cells, Cultured Viral Proteins - genetics
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