Shortcut Method for Extraction of Staphylococcus aureus DNA from Blood Cultures and Conventional Cultures for Use in Real-Time PCR Assays
PCR allows rapid determination of both the presence of Staphylococcus aureus and of methicillin resistance. I present here a simple method for direct extraction from blood cultures or colonies for use in real-time PCR, eliminating tedious, time-consuming methods of DNA extraction which may otherwise...
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| Veröffentlicht in: | Journal of Clinical Microbiology 2005-06, Vol.43 (6), p.2932-2933 |
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| container_end_page | 2933 |
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| container_issue | 6 |
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| container_title | Journal of Clinical Microbiology |
| container_volume | 43 |
| creator | Adams, D. Nicholas |
| description | PCR allows rapid determination of both the presence of Staphylococcus aureus and of methicillin resistance. I present here a simple method for direct extraction from blood cultures or colonies for use in real-time PCR, eliminating tedious, time-consuming methods of DNA extraction which may otherwise deter microbiology labs from offering PCR. |
| doi_str_mv | 10.1128/JCM.43.6.2932-2933.2005 |
| format | Article |
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Nicholas</creator><creatorcontrib>Adams, D. Nicholas</creatorcontrib><description>PCR allows rapid determination of both the presence of Staphylococcus aureus and of methicillin resistance. I present here a simple method for direct extraction from blood cultures or colonies for use in real-time PCR, eliminating tedious, time-consuming methods of DNA extraction which may otherwise deter microbiology labs from offering PCR.</description><identifier>ISSN: 0095-1137</identifier><identifier>EISSN: 1098-660X</identifier><identifier>DOI: 10.1128/JCM.43.6.2932-2933.2005</identifier><identifier>PMID: 15956423</identifier><identifier>CODEN: JCMIDW</identifier><language>eng</language><publisher>Washington, DC: American Society for Microbiology</publisher><subject>Bacteriology ; Biological and medical sciences ; Blood - microbiology ; Culture Media ; DNA, Bacterial - genetics ; DNA, Bacterial - isolation & purification ; Fundamental and applied biological sciences. 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Nicholas</creatorcontrib><title>Shortcut Method for Extraction of Staphylococcus aureus DNA from Blood Cultures and Conventional Cultures for Use in Real-Time PCR Assays</title><title>Journal of Clinical Microbiology</title><addtitle>J Clin Microbiol</addtitle><description>PCR allows rapid determination of both the presence of Staphylococcus aureus and of methicillin resistance. I present here a simple method for direct extraction from blood cultures or colonies for use in real-time PCR, eliminating tedious, time-consuming methods of DNA extraction which may otherwise deter microbiology labs from offering PCR.</description><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Blood - microbiology</subject><subject>Culture Media</subject><subject>DNA, Bacterial - genetics</subject><subject>DNA, Bacterial - isolation & purification</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Infectious diseases</subject><subject>Medical sciences</subject><subject>Methicillin Resistance - genetics</subject><subject>Microbiology</subject><subject>Miscellaneous</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Staphylococcus aureus - drug effects</subject><subject>Staphylococcus aureus - genetics</subject><subject>Staphylococcus aureus - isolation & purification</subject><issn>0095-1137</issn><issn>1098-660X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1u1DAUhSMEotPCK1CzgF2C_-NskKah_KkF1OlI7CyPx56kcuLBTlrmEXhrHM2IgRUbX9nnu8f36mTZOYIFQli8-VxfF5QUvMAVwXk6SIEhZI-yGYKVyDmH3x9nMwgrliNEypPsNMY7CBGljD3NThCrGKeYzLJfi8aHQY8DuDZD49fA-gAufw5B6aH1PfAWLAa1bXbOa6_1GIEag0nl3Zc5sMF34ML51FaPbkhCkvt08f296ad-5Y7K5LyMBrQ9uDHK5bdtZ8C3-gbMY1S7-Cx7YpWL5vmhnmXL95e39cf86uuHT_X8KtdMwCGvtC4FpoJQi1dM6WpNSkwrjKyoMBN4peEK4XV6t5rjtSmtVpbq0tqKc0QwOcve7n2346oza50GDcrJbWg7FXbSq1b-q_RtIzf-XiLEkChpMnh9MAj-x2jiILs2auOc6o0fo-RlioMy_F8QlZxwRlECyz2og48xGPtnGgTllLdMeUtKJJdT3tNB5JR36nzx9zLHvkPACXh1AFTUytmget3GI8cF4xxPS73cc027aR7aYKSKnbzT3fHbxJzvGau8VJuQfJYLDBGBCEIs0tq_AeK9yqU</recordid><startdate>20050601</startdate><enddate>20050601</enddate><creator>Adams, D. 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Psychology</topic><topic>Humans</topic><topic>Infectious diseases</topic><topic>Medical sciences</topic><topic>Methicillin Resistance - genetics</topic><topic>Microbiology</topic><topic>Miscellaneous</topic><topic>Polymerase Chain Reaction - methods</topic><topic>Staphylococcus aureus - drug effects</topic><topic>Staphylococcus aureus - genetics</topic><topic>Staphylococcus aureus - isolation & purification</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Adams, D. 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Nicholas</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Shortcut Method for Extraction of Staphylococcus aureus DNA from Blood Cultures and Conventional Cultures for Use in Real-Time PCR Assays</atitle><jtitle>Journal of Clinical Microbiology</jtitle><addtitle>J Clin Microbiol</addtitle><date>2005-06-01</date><risdate>2005</risdate><volume>43</volume><issue>6</issue><spage>2932</spage><epage>2933</epage><pages>2932-2933</pages><issn>0095-1137</issn><eissn>1098-660X</eissn><coden>JCMIDW</coden><abstract>PCR allows rapid determination of both the presence of Staphylococcus aureus and of methicillin resistance. 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| identifier | ISSN: 0095-1137 |
| ispartof | Journal of Clinical Microbiology, 2005-06, Vol.43 (6), p.2932-2933 |
| issn | 0095-1137 1098-660X |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_1151874 |
| source | American Society for Microbiology; MEDLINE; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Bacteriology Biological and medical sciences Blood - microbiology Culture Media DNA, Bacterial - genetics DNA, Bacterial - isolation & purification Fundamental and applied biological sciences. Psychology Humans Infectious diseases Medical sciences Methicillin Resistance - genetics Microbiology Miscellaneous Polymerase Chain Reaction - methods Staphylococcus aureus - drug effects Staphylococcus aureus - genetics Staphylococcus aureus - isolation & purification |
| title | Shortcut Method for Extraction of Staphylococcus aureus DNA from Blood Cultures and Conventional Cultures for Use in Real-Time PCR Assays |
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