Enhanced Golden Gate Assembly: evaluating overhang strength for improved ligation efficiency

Molecular cloning, a routine yet essential technique, relies heavily on efficient ligation, which can be significantly improved using Golden Gate Assembly (GGA). A key component of GGA is the use of type IIS enzymes, which uniquely cleave downstream of their recognition sequences to generate various...

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Veröffentlicht in:	Nucleic acids research 2024-10, Vol.52 (19), p.e95-e95
Hauptverfasser:	Strzelecki, Patryk, Joly, Nicolas, Hébraud, Pascal, Hoffmann, Elise, Cech, Grzegorz M, Kloska, Anna, Busi, Florent, Grange, Wilfried
Format:	Artikel
Sprache:	eng
Schlagworte:	Cloning, Molecular - methods DNA - chemistry DNA - genetics DNA - metabolism DNA Ligases - metabolism Life Sciences Methods
Online-Zugang:	Volltext
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