Single-Cell Microfluidics: A Primer for Microbiologists
Recent advances in microfluidic technology have made it possible to image live bacterial cells with a high degree of precision and control. In particular, single-cell microfluidic designs have created new opportunities to study phenotypic variation in bacterial populations. However, the development...
Gespeichert in:
| Veröffentlicht in: | The journal of physical chemistry. B 2024-10, Vol.128 (42), p.10311-10328 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 10328 |
|---|---|
| container_issue | 42 |
| container_start_page | 10311 |
| container_title | The journal of physical chemistry. B |
| container_volume | 128 |
| creator | Ripandelli, Remy A. A. van Oijen, Antoine M. Robinson, Andrew |
| description | Recent advances in microfluidic technology have made it possible to image live bacterial cells with a high degree of precision and control. In particular, single-cell microfluidic designs have created new opportunities to study phenotypic variation in bacterial populations. However, the development and use of microfluidic devices require specialized resources, and these can be practical barriers to entry for microbiologists. With this review, our intentions are to help demystify the design, construction, and application of microfluidics. Our approach is to present design elements as building blocks from which a multitude of microfluidics applications can be imagined by the microbiologist. |
| doi_str_mv | 10.1021/acs.jpcb.4c02746 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_11514030</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>3116339406</sourcerecordid><originalsourceid>FETCH-LOGICAL-a317t-4f2c969d9129b33aefacfc4e6460e424e03b248ad717d68b8b73b6331ce10cf63</originalsourceid><addsrcrecordid>eNp1kEtPAyEUhYnR2PrYuzJdunAqr8KMG9M0vpIaTdQ1AQYqzXSoMGPiv5c6Y6MLFwTIPfecez8AThAcI4jRhdRxvFxrNaYaYk7ZDhiiCYZZOny3fzME2QAcxLiEEE9wzvbBgBQ0fTgfAv7s6kVlspmpqtGD08HbqnWl0_FyNB09BbcyYWR96GrK-covXGziEdizsormuL8PwevN9cvsLps_3t7PpvNMEsSbjFqsC1aUBcKFIkQaK7XV1DDKoKGYGkgUprksOeIly1WuOFGMEKQNgtoycgiuOt91q1am1KZugqzEOg0mw6fw0om_ldq9iYX_EAhNEIUEJoez3iH499bERqxc1GldWRvfRkEQSoEJyCYMdtK0aozB2G0OgmIDXCTgYgNc9MBTy-nv-bYNP4ST4LwTfLf6NtQJ1_9-X3LjjKs</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>3116339406</pqid></control><display><type>article</type><title>Single-Cell Microfluidics: A Primer for Microbiologists</title><source>MEDLINE</source><source>ACS Publications</source><creator>Ripandelli, Remy A. A. ; van Oijen, Antoine M. ; Robinson, Andrew</creator><creatorcontrib>Ripandelli, Remy A. A. ; van Oijen, Antoine M. ; Robinson, Andrew</creatorcontrib><description>Recent advances in microfluidic technology have made it possible to image live bacterial cells with a high degree of precision and control. In particular, single-cell microfluidic designs have created new opportunities to study phenotypic variation in bacterial populations. However, the development and use of microfluidic devices require specialized resources, and these can be practical barriers to entry for microbiologists. With this review, our intentions are to help demystify the design, construction, and application of microfluidics. Our approach is to present design elements as building blocks from which a multitude of microfluidics applications can be imagined by the microbiologist.</description><identifier>ISSN: 1520-6106</identifier><identifier>ISSN: 1520-5207</identifier><identifier>EISSN: 1520-5207</identifier><identifier>DOI: 10.1021/acs.jpcb.4c02746</identifier><identifier>PMID: 39400277</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>Bacteria - cytology ; Lab-On-A-Chip Devices ; Microfluidic Analytical Techniques - instrumentation ; Microfluidics - methods ; Review ; Single-Cell Analysis</subject><ispartof>The journal of physical chemistry. B, 2024-10, Vol.128 (42), p.10311-10328</ispartof><rights>2024 The Authors. Published by American Chemical Society</rights><rights>2024 The Authors. Published by American Chemical Society 2024 The Authors</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-a317t-4f2c969d9129b33aefacfc4e6460e424e03b248ad717d68b8b73b6331ce10cf63</cites><orcidid>0000-0002-1794-5161 ; 0009-0006-1695-0723</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/acs.jpcb.4c02746$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/acs.jpcb.4c02746$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>230,315,781,785,886,2766,27081,27929,27930,56743,56793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/39400277$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ripandelli, Remy A. A.</creatorcontrib><creatorcontrib>van Oijen, Antoine M.</creatorcontrib><creatorcontrib>Robinson, Andrew</creatorcontrib><title>Single-Cell Microfluidics: A Primer for Microbiologists</title><title>The journal of physical chemistry. B</title><addtitle>J. Phys. Chem. B</addtitle><description>Recent advances in microfluidic technology have made it possible to image live bacterial cells with a high degree of precision and control. In particular, single-cell microfluidic designs have created new opportunities to study phenotypic variation in bacterial populations. However, the development and use of microfluidic devices require specialized resources, and these can be practical barriers to entry for microbiologists. With this review, our intentions are to help demystify the design, construction, and application of microfluidics. Our approach is to present design elements as building blocks from which a multitude of microfluidics applications can be imagined by the microbiologist.</description><subject>Bacteria - cytology</subject><subject>Lab-On-A-Chip Devices</subject><subject>Microfluidic Analytical Techniques - instrumentation</subject><subject>Microfluidics - methods</subject><subject>Review</subject><subject>Single-Cell Analysis</subject><issn>1520-6106</issn><issn>1520-5207</issn><issn>1520-5207</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kEtPAyEUhYnR2PrYuzJdunAqr8KMG9M0vpIaTdQ1AQYqzXSoMGPiv5c6Y6MLFwTIPfecez8AThAcI4jRhdRxvFxrNaYaYk7ZDhiiCYZZOny3fzME2QAcxLiEEE9wzvbBgBQ0fTgfAv7s6kVlspmpqtGD08HbqnWl0_FyNB09BbcyYWR96GrK-covXGziEdizsormuL8PwevN9cvsLps_3t7PpvNMEsSbjFqsC1aUBcKFIkQaK7XV1DDKoKGYGkgUprksOeIly1WuOFGMEKQNgtoycgiuOt91q1am1KZugqzEOg0mw6fw0om_ldq9iYX_EAhNEIUEJoez3iH499bERqxc1GldWRvfRkEQSoEJyCYMdtK0aozB2G0OgmIDXCTgYgNc9MBTy-nv-bYNP4ST4LwTfLf6NtQJ1_9-X3LjjKs</recordid><startdate>20241024</startdate><enddate>20241024</enddate><creator>Ripandelli, Remy A. A.</creator><creator>van Oijen, Antoine M.</creator><creator>Robinson, Andrew</creator><general>American Chemical Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-1794-5161</orcidid><orcidid>https://orcid.org/0009-0006-1695-0723</orcidid></search><sort><creationdate>20241024</creationdate><title>Single-Cell Microfluidics: A Primer for Microbiologists</title><author>Ripandelli, Remy A. A. ; van Oijen, Antoine M. ; Robinson, Andrew</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a317t-4f2c969d9129b33aefacfc4e6460e424e03b248ad717d68b8b73b6331ce10cf63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Bacteria - cytology</topic><topic>Lab-On-A-Chip Devices</topic><topic>Microfluidic Analytical Techniques - instrumentation</topic><topic>Microfluidics - methods</topic><topic>Review</topic><topic>Single-Cell Analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ripandelli, Remy A. A.</creatorcontrib><creatorcontrib>van Oijen, Antoine M.</creatorcontrib><creatorcontrib>Robinson, Andrew</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The journal of physical chemistry. B</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ripandelli, Remy A. A.</au><au>van Oijen, Antoine M.</au><au>Robinson, Andrew</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Single-Cell Microfluidics: A Primer for Microbiologists</atitle><jtitle>The journal of physical chemistry. B</jtitle><addtitle>J. Phys. Chem. B</addtitle><date>2024-10-24</date><risdate>2024</risdate><volume>128</volume><issue>42</issue><spage>10311</spage><epage>10328</epage><pages>10311-10328</pages><issn>1520-6106</issn><issn>1520-5207</issn><eissn>1520-5207</eissn><abstract>Recent advances in microfluidic technology have made it possible to image live bacterial cells with a high degree of precision and control. In particular, single-cell microfluidic designs have created new opportunities to study phenotypic variation in bacterial populations. However, the development and use of microfluidic devices require specialized resources, and these can be practical barriers to entry for microbiologists. With this review, our intentions are to help demystify the design, construction, and application of microfluidics. Our approach is to present design elements as building blocks from which a multitude of microfluidics applications can be imagined by the microbiologist.</abstract><cop>United States</cop><pub>American Chemical Society</pub><pmid>39400277</pmid><doi>10.1021/acs.jpcb.4c02746</doi><tpages>18</tpages><orcidid>https://orcid.org/0000-0002-1794-5161</orcidid><orcidid>https://orcid.org/0009-0006-1695-0723</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1520-6106 |
| ispartof | The journal of physical chemistry. B, 2024-10, Vol.128 (42), p.10311-10328 |
| issn | 1520-6106 1520-5207 1520-5207 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_11514030 |
| source | MEDLINE; ACS Publications |
| subjects | Bacteria - cytology Lab-On-A-Chip Devices Microfluidic Analytical Techniques - instrumentation Microfluidics - methods Review Single-Cell Analysis |
| title | Single-Cell Microfluidics: A Primer for Microbiologists |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-14T16%3A13%3A04IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Single-Cell%20Microfluidics:%20A%20Primer%20for%20Microbiologists&rft.jtitle=The%20journal%20of%20physical%20chemistry.%20B&rft.au=Ripandelli,%20Remy%20A.%20A.&rft.date=2024-10-24&rft.volume=128&rft.issue=42&rft.spage=10311&rft.epage=10328&rft.pages=10311-10328&rft.issn=1520-6106&rft.eissn=1520-5207&rft_id=info:doi/10.1021/acs.jpcb.4c02746&rft_dat=%3Cproquest_pubme%3E3116339406%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=3116339406&rft_id=info:pmid/39400277&rfr_iscdi=true |