Roots applicable, high sensitivity and specificity assay for the detection of Candidatus Liberibacter asiaticus in citrus roots and fruits
Candidatus Liberibacter asiaticus (CLas), a phloem-limited Gram-negative bacterium, is associated with citrus huanglongbing (HLB), which is one of the most destructive diseases currently threatening citrus production worldwide. No effective treatment for HLB is currently available. Effective prevent...
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| Veröffentlicht in: | Plant Biotechnology 2024/03/25, Vol.41(1), pp.27-34 |
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| creator | Zhong, Zecheng Chen, Yu Liu, Jinhua Wang, Wei Zhou, Feng Hu, Liu Zhang, Jinlian Chen, Tingsu Xiang, Jiyu Li, Tingdong Wang, Yingbin Zhang, Shiyin Ge, Shengxiang Zhang, Jun Xia, Ningshao |
| description | Candidatus Liberibacter asiaticus (CLas), a phloem-limited Gram-negative bacterium, is associated with citrus huanglongbing (HLB), which is one of the most destructive diseases currently threatening citrus production worldwide. No effective treatment for HLB is currently available. Effective prevention and control in the initial stage can block the spread and disease progression of HLB. Herein, we developed a co-detection assay for the 16S rDNA and 16S rRNA of CLas, the sensitivity of the co-detection assay was significantly increased over that of the single CLas DNA detection system. Beyond this, we found that the co-detection assay was a better fit to the root samples with higher population abundance than the previous reported detection system because it has a better specificity. Moreover, we found that the contents of 16S rRNA of CLas in citrus roots and fruits are significantly higher than that in leaves, which suggests that the time of HLB diagnosis is probably earlier by using these special tissues and the replication of CLas may become more active in these tissues, further suggested that the significance of study the mechanism of infection, prevention and control of HLB staring from these tissues. |
| doi_str_mv | 10.5511/plantbiotechnology.23.1129a |
| format | Article |
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No effective treatment for HLB is currently available. Effective prevention and control in the initial stage can block the spread and disease progression of HLB. Herein, we developed a co-detection assay for the 16S rDNA and 16S rRNA of CLas, the sensitivity of the co-detection assay was significantly increased over that of the single CLas DNA detection system. Beyond this, we found that the co-detection assay was a better fit to the root samples with higher population abundance than the previous reported detection system because it has a better specificity. Moreover, we found that the contents of 16S rRNA of CLas in citrus roots and fruits are significantly higher than that in leaves, which suggests that the time of HLB diagnosis is probably earlier by using these special tissues and the replication of CLas may become more active in these tissues, further suggested that the significance of study the mechanism of infection, prevention and control of HLB staring from these tissues.</description><identifier>ISSN: 1342-4580</identifier><identifier>EISSN: 1347-6114</identifier><identifier>DOI: 10.5511/plantbiotechnology.23.1129a</identifier><identifier>PMID: 39464864</identifier><language>eng</language><publisher>Japan: Japanese Society for Plant Biotechnology</publisher><subject>16S rRNA ; Active control ; Assaying ; Candidatus Liberibacter asiaticus ; Disease control ; early diagnosis ; Fruits ; Gram-negative bacteria ; HLB ; Original Paper ; Plant bacterial diseases ; Prevention ; Roots ; rRNA 16S ; Sensitivity</subject><ispartof>Plant Biotechnology, 2024/03/25, Vol.41(1), pp.27-34</ispartof><rights>2024 Japanese Society for Plant Biotechnology</rights><rights>2024 Japanese Society for Plant Biotechnology.</rights><rights>2024. This work is published under https://creativecommons.org/licenses/by/4.0/ (the “License”). 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No effective treatment for HLB is currently available. Effective prevention and control in the initial stage can block the spread and disease progression of HLB. Herein, we developed a co-detection assay for the 16S rDNA and 16S rRNA of CLas, the sensitivity of the co-detection assay was significantly increased over that of the single CLas DNA detection system. Beyond this, we found that the co-detection assay was a better fit to the root samples with higher population abundance than the previous reported detection system because it has a better specificity. Moreover, we found that the contents of 16S rRNA of CLas in citrus roots and fruits are significantly higher than that in leaves, which suggests that the time of HLB diagnosis is probably earlier by using these special tissues and the replication of CLas may become more active in these tissues, further suggested that the significance of study the mechanism of infection, prevention and control of HLB staring from these tissues.</description><subject>16S rRNA</subject><subject>Active control</subject><subject>Assaying</subject><subject>Candidatus Liberibacter asiaticus</subject><subject>Disease control</subject><subject>early diagnosis</subject><subject>Fruits</subject><subject>Gram-negative bacteria</subject><subject>HLB</subject><subject>Original Paper</subject><subject>Plant bacterial diseases</subject><subject>Prevention</subject><subject>Roots</subject><subject>rRNA 16S</subject><subject>Sensitivity</subject><issn>1342-4580</issn><issn>1347-6114</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNptkc-O0zAQxiMEYpeFV0CW9sKBFI_tOIk4IFTtAlIlBIKz5TqTxlVqB9tZqa_AU-O2uxX_LvbY_s03M_6K4hrooqoA3kyjdmltfUIzOD_6zX7B-AKAtfpRcQlc1KUEEI-PMStF1dCL4lmMW0pZBZQ9LS54K6RopLgsfn71PkWip2m0Rq9HfE0GuxlIRBdtsnc27Yl2HYkTGttbczzHqPek94GkAUmHuZFkvSO-J8vM2k6nOZKVXWOwa20ShpxidbImX1tHskjIUThVzuJ9mG2Kz4snvR4jvrjfr4rvtzfflh_L1ecPn5bvV6WRTKSyliLPgb3R2IFgUkpTG9ZRZnjN2kq0PYMG6vxVbdUwpiXrkaNsQDOuuaH8qnh30p3m9Q47gy4FPaop2J0Oe-W1VX--ODuojb9TABWlVXtQeHWvEPyPGWNSOxsNjtkY9HNUHBiwhkHNM3r9F7r1c3B5PsVpQwFozVmm3p4oE3yMAftzN0DVwXT1r-mKcXU0PWe__H2gc-6Dyxn4cgK2MekNngEdsicj_k9cgILD8lDkzJpBB4WO_wKHpNBC</recordid><startdate>20240325</startdate><enddate>20240325</enddate><creator>Zhong, Zecheng</creator><creator>Chen, Yu</creator><creator>Liu, Jinhua</creator><creator>Wang, Wei</creator><creator>Zhou, Feng</creator><creator>Hu, Liu</creator><creator>Zhang, Jinlian</creator><creator>Chen, Tingsu</creator><creator>Xiang, Jiyu</creator><creator>Li, Tingdong</creator><creator>Wang, Yingbin</creator><creator>Zhang, Shiyin</creator><creator>Ge, Shengxiang</creator><creator>Zhang, Jun</creator><creator>Xia, Ningshao</creator><general>Japanese Society for Plant Biotechnology</general><general>Japan Science and Technology Agency</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20240325</creationdate><title>Roots applicable, high sensitivity and specificity assay for the detection of Candidatus Liberibacter asiaticus in citrus roots and fruits</title><author>Zhong, Zecheng ; Chen, Yu ; Liu, Jinhua ; Wang, Wei ; Zhou, Feng ; Hu, Liu ; Zhang, Jinlian ; Chen, Tingsu ; Xiang, Jiyu ; Li, Tingdong ; Wang, Yingbin ; Zhang, Shiyin ; Ge, Shengxiang ; Zhang, Jun ; Xia, Ningshao</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c624t-764025efcaed142666c7c2d02c3729549f2181755195822a62fe3e681a23a3c03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>16S rRNA</topic><topic>Active control</topic><topic>Assaying</topic><topic>Candidatus Liberibacter asiaticus</topic><topic>Disease control</topic><topic>early diagnosis</topic><topic>Fruits</topic><topic>Gram-negative bacteria</topic><topic>HLB</topic><topic>Original Paper</topic><topic>Plant bacterial diseases</topic><topic>Prevention</topic><topic>Roots</topic><topic>rRNA 16S</topic><topic>Sensitivity</topic><toplevel>online_resources</toplevel><creatorcontrib>Zhong, Zecheng</creatorcontrib><creatorcontrib>Chen, Yu</creatorcontrib><creatorcontrib>Liu, Jinhua</creatorcontrib><creatorcontrib>Wang, Wei</creatorcontrib><creatorcontrib>Zhou, Feng</creatorcontrib><creatorcontrib>Hu, Liu</creatorcontrib><creatorcontrib>Zhang, Jinlian</creatorcontrib><creatorcontrib>Chen, Tingsu</creatorcontrib><creatorcontrib>Xiang, Jiyu</creatorcontrib><creatorcontrib>Li, Tingdong</creatorcontrib><creatorcontrib>Wang, Yingbin</creatorcontrib><creatorcontrib>Zhang, Shiyin</creatorcontrib><creatorcontrib>Ge, Shengxiang</creatorcontrib><creatorcontrib>Zhang, Jun</creatorcontrib><creatorcontrib>Xia, Ningshao</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Plant Biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhong, Zecheng</au><au>Chen, Yu</au><au>Liu, Jinhua</au><au>Wang, Wei</au><au>Zhou, Feng</au><au>Hu, Liu</au><au>Zhang, Jinlian</au><au>Chen, Tingsu</au><au>Xiang, Jiyu</au><au>Li, Tingdong</au><au>Wang, Yingbin</au><au>Zhang, Shiyin</au><au>Ge, Shengxiang</au><au>Zhang, Jun</au><au>Xia, Ningshao</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Roots applicable, high sensitivity and specificity assay for the detection of Candidatus Liberibacter asiaticus in citrus roots and fruits</atitle><jtitle>Plant Biotechnology</jtitle><addtitle>Plant Biotechnol (Tokyo)</addtitle><date>2024-03-25</date><risdate>2024</risdate><volume>41</volume><issue>1</issue><spage>27</spage><epage>34</epage><pages>27-34</pages><artnum>23.1129a</artnum><issn>1342-4580</issn><eissn>1347-6114</eissn><abstract>Candidatus Liberibacter asiaticus (CLas), a phloem-limited Gram-negative bacterium, is associated with citrus huanglongbing (HLB), which is one of the most destructive diseases currently threatening citrus production worldwide. No effective treatment for HLB is currently available. Effective prevention and control in the initial stage can block the spread and disease progression of HLB. Herein, we developed a co-detection assay for the 16S rDNA and 16S rRNA of CLas, the sensitivity of the co-detection assay was significantly increased over that of the single CLas DNA detection system. Beyond this, we found that the co-detection assay was a better fit to the root samples with higher population abundance than the previous reported detection system because it has a better specificity. Moreover, we found that the contents of 16S rRNA of CLas in citrus roots and fruits are significantly higher than that in leaves, which suggests that the time of HLB diagnosis is probably earlier by using these special tissues and the replication of CLas may become more active in these tissues, further suggested that the significance of study the mechanism of infection, prevention and control of HLB staring from these tissues.</abstract><cop>Japan</cop><pub>Japanese Society for Plant Biotechnology</pub><pmid>39464864</pmid><doi>10.5511/plantbiotechnology.23.1129a</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | 16S rRNA Active control Assaying Candidatus Liberibacter asiaticus Disease control early diagnosis Fruits Gram-negative bacteria HLB Original Paper Plant bacterial diseases Prevention Roots rRNA 16S Sensitivity |
| title | Roots applicable, high sensitivity and specificity assay for the detection of Candidatus Liberibacter asiaticus in citrus roots and fruits |
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