Stability of mutant actins

Mutants of the Drosophila Act88F actin gene were transcribed and translated in vitro and their relative stabilities were examined using urea gradient gel electrophoresis. Most of the mutant actins (E334K, E364K, G366D, G368E and R372H) were as stable as the wild-type. V339I had a slight decrease in...

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Veröffentlicht in:	Biochemical journal 1991-02, Vol.274 (1), p.301-303
Hauptverfasser:	Drummond, D.D, Hennessey, E.S, Sparrow, J.C
Format:	Artikel
Sprache:	eng
Schlagworte:	actin Actins - chemistry Actins - genetics Analytical, structural and metabolic biochemistry Animals Biological and medical sciences Contractile proteins Drosophila Drug Stability Electrophoresis, Polyacrylamide Gel Fundamental and applied biological sciences. Psychology genes Holoproteins mutants Mutation Protein Biosynthesis Protein Denaturation Proteins Rabbits stability transcription (genetics) Transcription, Genetic translation Urea
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