Use of ATP, dATP and their alpha-thio derivatives to study DNA ligase adenylation

Bacteriophage-T4 and human type I DNA ligases were found capable of self-adenylating upon exposure to both ribo- and deoxyribo-[alpha-35S]thio-ATP. However, the joining reaction does not take place in the presence of the deoxyribotriphosphates. Enzyme adenylation is reversed in all cases by an exces...

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Veröffentlicht in:	Biochemical journal 1990-10, Vol.271 (1), p.265-268
Hauptverfasser:	Montecucco, A, Lestingi, M, Pedrali-Noy, G, Spadari, S, Ciarrocchi, G
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenosine Triphosphate - analogs & derivatives Adenosine Triphosphate - metabolism Antibiotics, Antineoplastic - pharmacology Collodion Deoxyadenine Nucleotides - metabolism DNA Ligases - antagonists & inhibitors DNA Ligases - metabolism HeLa Cells - enzymology Humans phage T4 Phosphates - pharmacology T-Phages - enzymology Thionucleotides - metabolism
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