Purification and biosynthesis of cottonseed (Gossypium hirsutum L.) catalase

As part of our research on peroxisome biogenesis, catalase was purified from cotyledons of dark-grown cotton (Gossypium hirsutum L.) seedlings and monospecific antibodies were raised in rabbits. Purified catalase appeared as three distinct electrophoretic forms in non-denaturing gels and as a single...

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Veröffentlicht in:	Biochemical journal 1988-04, Vol.251 (1), p.147-155
Hauptverfasser:	Kunce, C.M, Trelease, R.N, Turley, R.B
Format:	Artikel
Sprache:	eng
Schlagworte:	biosynthesis catalase Catalase - antagonists & inhibitors Catalase - biosynthesis Catalase - isolation & purification Centrifugation, Density Gradient Chromatography, Liquid cottonseed Electrophoresis, Polyacrylamide Gel enzyme activity Gossypium - enzymology Gossypium hirsutum Immunodiffusion Methionine - metabolism Microbodies - enzymology Protein Biosynthesis purification ribosomes
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