Early conformational changes and activity modulation induced by guanidinium chloride on intestinal alkaline phosphatase

Moderate concentrations of guanidinium chloride induce both instantaneous and time-dependent modifications of the catalytic and optical properties of intestinal alkaline phosphatase, which undergoes consecutive conformational transitions at about 0.05 M, 0.25 M and 1.0 M denaturant. A paradoxical ac...

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Veröffentlicht in:	Biochemical journal 1987-12, Vol.248 (2), p.551-556
Hauptverfasser:	Miggiano, G A, Mordente, A, Pischiutta, M G, Martorana, G E, Castelli, A
Format:	Artikel
Sprache:	eng
Schlagworte:	Alkaline Phosphatase - metabolism Animals Cattle Enzyme Activation - drug effects Guanidine Guanidines - pharmacology Hydrogen-Ion Concentration Intestines - enzymology Protein Conformation - drug effects Spectrometry, Fluorescence Spectrophotometry
Online-Zugang:	Volltext
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