Effect of adenosine deaminase, N6-phenylisopropyladenosine and hypothyroidism on the responsiveness of rat brown adipocytes to noradrenaline
Adenosine deaminase (1 unit/ml) potentiated the lipolytic action of noradrenaline in adipocytes isolated from brown adipose tissue of 1- and 6-week-old rats by decreasing the EC50 (concn. giving 50% of maximal effect) for noradrenaline by 3-4-fold. With cells from neonatal rabbit tissue, adenosine d...
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| Veröffentlicht in: | Biochemical journal 1986-09, Vol.238 (2), p.395-403 |
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| creator | Woodward, J A Saggerson, E D |
| description | Adenosine deaminase (1 unit/ml) potentiated the lipolytic action of noradrenaline in adipocytes isolated from brown adipose tissue of 1- and 6-week-old rats by decreasing the EC50 (concn. giving 50% of maximal effect) for noradrenaline by 3-4-fold. With cells from neonatal rabbit tissue, adenosine deaminase only had a small, non-significant, effect on the EC50 for noradrenaline. Lipolysis in rat brown adipocytes was inhibited by low concentrations of N6-phenylisopropyladenosine (PIA). Rabbit cells were far less sensitive to PIA. PIA, prostaglandin E1 and nicotinate all inhibited noradrenaline-stimulated respiration in rat brown adipocytes. Hypothyroidism diminished the maximum response of respiration and lipolysis to noradrenaline in rat cells and increased the EC50 for noradrenaline. Responsiveness of lipolysis to noradrenaline was particularly decreased in hypothyroidism and was partially restored by addition of adenosine deaminase. Lipolysis in cells from hypothyroid rats was more sensitive to the anti-lipolytic action of PIA. Bordetella pertussis toxin increased lipolysis in the presence of PIA, suggesting an involvement of the Ni guanine-nucleotide-binding protein in the control of brown-adipocyte metabolism. |
| doi_str_mv | 10.1042/bj2380395 |
| format | Article |
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With cells from neonatal rabbit tissue, adenosine deaminase only had a small, non-significant, effect on the EC50 for noradrenaline. Lipolysis in rat brown adipocytes was inhibited by low concentrations of N6-phenylisopropyladenosine (PIA). Rabbit cells were far less sensitive to PIA. PIA, prostaglandin E1 and nicotinate all inhibited noradrenaline-stimulated respiration in rat brown adipocytes. Hypothyroidism diminished the maximum response of respiration and lipolysis to noradrenaline in rat cells and increased the EC50 for noradrenaline. Responsiveness of lipolysis to noradrenaline was particularly decreased in hypothyroidism and was partially restored by addition of adenosine deaminase. Lipolysis in cells from hypothyroid rats was more sensitive to the anti-lipolytic action of PIA. Bordetella pertussis toxin increased lipolysis in the presence of PIA, suggesting an involvement of the Ni guanine-nucleotide-binding protein in the control of brown-adipocyte metabolism.</description><identifier>ISSN: 0264-6021</identifier><identifier>EISSN: 1470-8728</identifier><identifier>DOI: 10.1042/bj2380395</identifier><identifier>PMID: 3800944</identifier><language>eng</language><publisher>England</publisher><subject>Adenosine - analogs & derivatives ; Adenosine Deaminase - pharmacology ; Adipose Tissue, Brown - drug effects ; Adipose Tissue, Brown - metabolism ; Alprostadil - pharmacology ; Animals ; Female ; Hypothyroidism - metabolism ; In Vitro Techniques ; Lipolysis - drug effects ; Male ; Niacin - pharmacology ; Norepinephrine - pharmacology ; Nucleoside Deaminases - pharmacology ; Oxygen Consumption - drug effects ; Pertussis Toxin ; Phenylisopropyladenosine - pharmacology ; Rats ; Rats, Inbred Strains ; Virulence Factors, Bordetella - pharmacology</subject><ispartof>Biochemical journal, 1986-09, Vol.238 (2), p.395-403</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1147149/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1147149/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3800944$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Woodward, J A</creatorcontrib><creatorcontrib>Saggerson, E D</creatorcontrib><title>Effect of adenosine deaminase, N6-phenylisopropyladenosine and hypothyroidism on the responsiveness of rat brown adipocytes to noradrenaline</title><title>Biochemical journal</title><addtitle>Biochem J</addtitle><description>Adenosine deaminase (1 unit/ml) potentiated the lipolytic action of noradrenaline in adipocytes isolated from brown adipose tissue of 1- and 6-week-old rats by decreasing the EC50 (concn. giving 50% of maximal effect) for noradrenaline by 3-4-fold. With cells from neonatal rabbit tissue, adenosine deaminase only had a small, non-significant, effect on the EC50 for noradrenaline. Lipolysis in rat brown adipocytes was inhibited by low concentrations of N6-phenylisopropyladenosine (PIA). Rabbit cells were far less sensitive to PIA. PIA, prostaglandin E1 and nicotinate all inhibited noradrenaline-stimulated respiration in rat brown adipocytes. Hypothyroidism diminished the maximum response of respiration and lipolysis to noradrenaline in rat cells and increased the EC50 for noradrenaline. Responsiveness of lipolysis to noradrenaline was particularly decreased in hypothyroidism and was partially restored by addition of adenosine deaminase. Lipolysis in cells from hypothyroid rats was more sensitive to the anti-lipolytic action of PIA. Bordetella pertussis toxin increased lipolysis in the presence of PIA, suggesting an involvement of the Ni guanine-nucleotide-binding protein in the control of brown-adipocyte metabolism.</description><subject>Adenosine - analogs & derivatives</subject><subject>Adenosine Deaminase - pharmacology</subject><subject>Adipose Tissue, Brown - drug effects</subject><subject>Adipose Tissue, Brown - metabolism</subject><subject>Alprostadil - pharmacology</subject><subject>Animals</subject><subject>Female</subject><subject>Hypothyroidism - metabolism</subject><subject>In Vitro Techniques</subject><subject>Lipolysis - drug effects</subject><subject>Male</subject><subject>Niacin - pharmacology</subject><subject>Norepinephrine - pharmacology</subject><subject>Nucleoside Deaminases - pharmacology</subject><subject>Oxygen Consumption - drug effects</subject><subject>Pertussis Toxin</subject><subject>Phenylisopropyladenosine - pharmacology</subject><subject>Rats</subject><subject>Rats, Inbred Strains</subject><subject>Virulence Factors, Bordetella - pharmacology</subject><issn>0264-6021</issn><issn>1470-8728</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1986</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkMtKxDAUhoMo43hZ-ABCVq6sJm3SNBtBhvECohtdlzQ9tRnapCYZpe_gQ9vBwcvqLM7H98GP0AklF5Sw9LJapVlBMsl30JwyQZJCpMUumpM0Z0lOUrqPDkJYEUIZYWSGZhNNJGNz9LlsGtARuwarGqwLxgKuQfXGqgDn-DFPhhbs2JngBu-GsfvFlK1xOw4utqN3pjahx87i2AL2EAZng3kHCyFs5F5FXHn3YaeMGZweIwQcHbbOq9qDVd1kPEJ7jeoCHG_vIXq5WT4v7pKHp9v7xfVDskoli4kUkgKjteAceCYLTqSSikqmKWM8S2kjGFeESZLphktdcVbVDeeaVoWiusgO0dW3d1hXPdQabPSqKwdveuXH0ilT_v9Y05av7r2k07iUyUlwthV497aGEMveBA1dpyy4dSiFSLNc5Bvw9G_pJ7HdP_sCGf-K-w</recordid><startdate>19860901</startdate><enddate>19860901</enddate><creator>Woodward, J A</creator><creator>Saggerson, E D</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19860901</creationdate><title>Effect of adenosine deaminase, N6-phenylisopropyladenosine and hypothyroidism on the responsiveness of rat brown adipocytes to noradrenaline</title><author>Woodward, J A ; Saggerson, E D</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-j294t-9791e41d755e5398509a9a194c1445321f745a04903cf59cb54bdf55c1b8a1c83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1986</creationdate><topic>Adenosine - analogs & derivatives</topic><topic>Adenosine Deaminase - pharmacology</topic><topic>Adipose Tissue, Brown - drug effects</topic><topic>Adipose Tissue, Brown - metabolism</topic><topic>Alprostadil - pharmacology</topic><topic>Animals</topic><topic>Female</topic><topic>Hypothyroidism - metabolism</topic><topic>In Vitro Techniques</topic><topic>Lipolysis - drug effects</topic><topic>Male</topic><topic>Niacin - pharmacology</topic><topic>Norepinephrine - pharmacology</topic><topic>Nucleoside Deaminases - pharmacology</topic><topic>Oxygen Consumption - drug effects</topic><topic>Pertussis Toxin</topic><topic>Phenylisopropyladenosine - pharmacology</topic><topic>Rats</topic><topic>Rats, Inbred Strains</topic><topic>Virulence Factors, Bordetella - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Woodward, J A</creatorcontrib><creatorcontrib>Saggerson, E D</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Biochemical journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Woodward, J A</au><au>Saggerson, E D</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of adenosine deaminase, N6-phenylisopropyladenosine and hypothyroidism on the responsiveness of rat brown adipocytes to noradrenaline</atitle><jtitle>Biochemical journal</jtitle><addtitle>Biochem J</addtitle><date>1986-09-01</date><risdate>1986</risdate><volume>238</volume><issue>2</issue><spage>395</spage><epage>403</epage><pages>395-403</pages><issn>0264-6021</issn><eissn>1470-8728</eissn><abstract>Adenosine deaminase (1 unit/ml) potentiated the lipolytic action of noradrenaline in adipocytes isolated from brown adipose tissue of 1- and 6-week-old rats by decreasing the EC50 (concn. giving 50% of maximal effect) for noradrenaline by 3-4-fold. With cells from neonatal rabbit tissue, adenosine deaminase only had a small, non-significant, effect on the EC50 for noradrenaline. Lipolysis in rat brown adipocytes was inhibited by low concentrations of N6-phenylisopropyladenosine (PIA). Rabbit cells were far less sensitive to PIA. PIA, prostaglandin E1 and nicotinate all inhibited noradrenaline-stimulated respiration in rat brown adipocytes. Hypothyroidism diminished the maximum response of respiration and lipolysis to noradrenaline in rat cells and increased the EC50 for noradrenaline. Responsiveness of lipolysis to noradrenaline was particularly decreased in hypothyroidism and was partially restored by addition of adenosine deaminase. Lipolysis in cells from hypothyroid rats was more sensitive to the anti-lipolytic action of PIA. Bordetella pertussis toxin increased lipolysis in the presence of PIA, suggesting an involvement of the Ni guanine-nucleotide-binding protein in the control of brown-adipocyte metabolism.</abstract><cop>England</cop><pmid>3800944</pmid><doi>10.1042/bj2380395</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Biochemical journal, 1986-09, Vol.238 (2), p.395-403 |
| issn | 0264-6021 1470-8728 |
| language | eng |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; PubMed Central; Alma/SFX Local Collection |
| subjects | Adenosine - analogs & derivatives Adenosine Deaminase - pharmacology Adipose Tissue, Brown - drug effects Adipose Tissue, Brown - metabolism Alprostadil - pharmacology Animals Female Hypothyroidism - metabolism In Vitro Techniques Lipolysis - drug effects Male Niacin - pharmacology Norepinephrine - pharmacology Nucleoside Deaminases - pharmacology Oxygen Consumption - drug effects Pertussis Toxin Phenylisopropyladenosine - pharmacology Rats Rats, Inbred Strains Virulence Factors, Bordetella - pharmacology |
| title | Effect of adenosine deaminase, N6-phenylisopropyladenosine and hypothyroidism on the responsiveness of rat brown adipocytes to noradrenaline |
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