Extraction, purification, identification and metabolism of 3',5'-cyclic UMP, 3',5'-cyclic IMP and 3',5'-cyclic dTMP from rat tissues

The large-scale extraction and partial purification of endogenous 3',5'-cyclic UMP, 3',5'-cyclic IMP and 3',5'-cyclic dTMP are described. Rat liver, kidney, heart, spleen and lung tissues were subjected to a sequential purification procedure involving freeze-clamping, p...

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Veröffentlicht in:	Biochemical journal 1986-06, Vol.236 (2), p.431-439
Hauptverfasser:	Newton, R P, Kingston, E E, Hakeem, N A, Salih, S G, Beynon, J H, Moyse, C D
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Cyclic IMP - isolation & purification Cyclic IMP - metabolism Inosine Nucleotides - metabolism Mass Spectrometry Nucleotides, Cyclic - isolation & purification Nucleotides, Cyclic - metabolism Rats Rats, Inbred Strains Thymidine Monophosphate - isolation & purification Thymidine Monophosphate - metabolism Thymine Nucleotides - metabolism Tissue Distribution Uracil Nucleotides - metabolism Uridine Monophosphate - isolation & purification Uridine Monophosphate - metabolism
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