Microcalorimetric measurements of heat production in brown adipocytes from control and cafeteria-fed rats
The effects of the sequential addition of glucose, noradrenaline, propranolol and oleic acid on the rates of O2 consumption and heat production by isolated interscapular brown adipocytes from control and cafeteria-fed rats were compared. Although the chemical agents produced very similar changes in...
Gespeichert in:
| Veröffentlicht in: | Biochemical journal 1986-04, Vol.235 (2), p.337-342 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 342 |
|---|---|
| container_issue | 2 |
| container_start_page | 337 |
| container_title | Biochemical journal |
| container_volume | 235 |
| creator | Clark, D.G Brinkman, M Neville, S.D |
| description | The effects of the sequential addition of glucose, noradrenaline, propranolol and oleic acid on the rates of O2 consumption and heat production by isolated interscapular brown adipocytes from control and cafeteria-fed rats were compared. Although the chemical agents produced very similar changes in oxidative metabolism, the actual rates of O2 uptake and heat output in adipocytes from the cafeteria-fed rats, when expressed per g dry wt. of cells, were approx. 65% less than those obtained with cells from the control rats. However, when the same results were expressed per 10(8) multiloccular brown adipocytes, rather than gravimetrically, rates of O2 consumption and heat production were equivalent. Further interpretation of these data is complicated, because the average volume of multiloccular brown adipocytes from cafeteria-fed rats was 2.5 times that for multiloccular cells from control animals. |
| doi_str_mv | 10.1042/bj2350337 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_1146692</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>76988095</sourcerecordid><originalsourceid>FETCH-LOGICAL-c460t-5bb6bffeb2810cd7a4bdc306386b9739c8294235ca8b03d2d8a49a24d2812bf63</originalsourceid><addsrcrecordid>eNpVkc1rFTEUxYMo9bW68A8QsxJcjObrZZKNIEWrUOlCuw43X23KzOSZZCr97xt5j4eFwF2cH-fm3IPQG0o-UiLYJ3vH-JZwPj5DGypGMqiRqedoQ5gUgySMvkSntd4RQgUR5ASd8FFQrsUGpZ_JlexgyiXNoZXk8BygriXMYWkV54hvAzS8K9mvrqW84LRgW_LfBYNPu-weWqg4ljxjl5dW8oRh8dhBDC2UBEMMHhdo9RV6EWGq4fVhnqHrb19_n38fLq8ufpx_uRyckKQNW2uljTFYpihxfgRhveNEciWtHrl2imnR0zpQlnDPvAKhgQnfeWaj5Gfo8953t9o5eNdjFJjMrueD8mAyJPNUWdKtucn3hlIhpWbd4P3BoOQ_a6jNzKm6ME2whLxWM0qtFNHbDn7Yg_2CtZYQj0soMf96McdeOvv2_18dyUMRXX-31yNkAzclVXP9ixHK-9NS9vyPRcOUuQ</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>76988095</pqid></control><display><type>article</type><title>Microcalorimetric measurements of heat production in brown adipocytes from control and cafeteria-fed rats</title><source>MEDLINE</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><creator>Clark, D.G ; Brinkman, M ; Neville, S.D</creator><creatorcontrib>Clark, D.G ; Brinkman, M ; Neville, S.D</creatorcontrib><description>The effects of the sequential addition of glucose, noradrenaline, propranolol and oleic acid on the rates of O2 consumption and heat production by isolated interscapular brown adipocytes from control and cafeteria-fed rats were compared. Although the chemical agents produced very similar changes in oxidative metabolism, the actual rates of O2 uptake and heat output in adipocytes from the cafeteria-fed rats, when expressed per g dry wt. of cells, were approx. 65% less than those obtained with cells from the control rats. However, when the same results were expressed per 10(8) multiloccular brown adipocytes, rather than gravimetrically, rates of O2 consumption and heat production were equivalent. Further interpretation of these data is complicated, because the average volume of multiloccular brown adipocytes from cafeteria-fed rats was 2.5 times that for multiloccular cells from control animals.</description><identifier>ISSN: 0264-6021</identifier><identifier>EISSN: 1470-8728</identifier><identifier>DOI: 10.1042/bj2350337</identifier><identifier>PMID: 3741394</identifier><language>eng</language><publisher>England</publisher><subject>adipocytes ; Adipose Tissue, Brown - cytology ; Adipose Tissue, Brown - drug effects ; Adipose Tissue, Brown - metabolism ; Animals ; brown adipose tissue ; cafeterias ; calorimeters ; Calorimetry ; Diet ; Glucose - pharmacology ; heat production ; Hot Temperature ; In Vitro Techniques ; Male ; Norepinephrine - pharmacology ; Oleic Acid ; Oleic Acids - pharmacology ; oxygen consumption ; Oxygen Consumption - drug effects ; Propranolol - pharmacology ; Rats ; Rats, Inbred Strains</subject><ispartof>Biochemical journal, 1986-04, Vol.235 (2), p.337-342</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c460t-5bb6bffeb2810cd7a4bdc306386b9739c8294235ca8b03d2d8a49a24d2812bf63</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1146692/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1146692/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3741394$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Clark, D.G</creatorcontrib><creatorcontrib>Brinkman, M</creatorcontrib><creatorcontrib>Neville, S.D</creatorcontrib><title>Microcalorimetric measurements of heat production in brown adipocytes from control and cafeteria-fed rats</title><title>Biochemical journal</title><addtitle>Biochem J</addtitle><description>The effects of the sequential addition of glucose, noradrenaline, propranolol and oleic acid on the rates of O2 consumption and heat production by isolated interscapular brown adipocytes from control and cafeteria-fed rats were compared. Although the chemical agents produced very similar changes in oxidative metabolism, the actual rates of O2 uptake and heat output in adipocytes from the cafeteria-fed rats, when expressed per g dry wt. of cells, were approx. 65% less than those obtained with cells from the control rats. However, when the same results were expressed per 10(8) multiloccular brown adipocytes, rather than gravimetrically, rates of O2 consumption and heat production were equivalent. Further interpretation of these data is complicated, because the average volume of multiloccular brown adipocytes from cafeteria-fed rats was 2.5 times that for multiloccular cells from control animals.</description><subject>adipocytes</subject><subject>Adipose Tissue, Brown - cytology</subject><subject>Adipose Tissue, Brown - drug effects</subject><subject>Adipose Tissue, Brown - metabolism</subject><subject>Animals</subject><subject>brown adipose tissue</subject><subject>cafeterias</subject><subject>calorimeters</subject><subject>Calorimetry</subject><subject>Diet</subject><subject>Glucose - pharmacology</subject><subject>heat production</subject><subject>Hot Temperature</subject><subject>In Vitro Techniques</subject><subject>Male</subject><subject>Norepinephrine - pharmacology</subject><subject>Oleic Acid</subject><subject>Oleic Acids - pharmacology</subject><subject>oxygen consumption</subject><subject>Oxygen Consumption - drug effects</subject><subject>Propranolol - pharmacology</subject><subject>Rats</subject><subject>Rats, Inbred Strains</subject><issn>0264-6021</issn><issn>1470-8728</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1986</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkc1rFTEUxYMo9bW68A8QsxJcjObrZZKNIEWrUOlCuw43X23KzOSZZCr97xt5j4eFwF2cH-fm3IPQG0o-UiLYJ3vH-JZwPj5DGypGMqiRqedoQ5gUgySMvkSntd4RQgUR5ASd8FFQrsUGpZ_JlexgyiXNoZXk8BygriXMYWkV54hvAzS8K9mvrqW84LRgW_LfBYNPu-weWqg4ljxjl5dW8oRh8dhBDC2UBEMMHhdo9RV6EWGq4fVhnqHrb19_n38fLq8ufpx_uRyckKQNW2uljTFYpihxfgRhveNEciWtHrl2imnR0zpQlnDPvAKhgQnfeWaj5Gfo8953t9o5eNdjFJjMrueD8mAyJPNUWdKtucn3hlIhpWbd4P3BoOQ_a6jNzKm6ME2whLxWM0qtFNHbDn7Yg_2CtZYQj0soMf96McdeOvv2_18dyUMRXX-31yNkAzclVXP9ixHK-9NS9vyPRcOUuQ</recordid><startdate>19860415</startdate><enddate>19860415</enddate><creator>Clark, D.G</creator><creator>Brinkman, M</creator><creator>Neville, S.D</creator><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19860415</creationdate><title>Microcalorimetric measurements of heat production in brown adipocytes from control and cafeteria-fed rats</title><author>Clark, D.G ; Brinkman, M ; Neville, S.D</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c460t-5bb6bffeb2810cd7a4bdc306386b9739c8294235ca8b03d2d8a49a24d2812bf63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1986</creationdate><topic>adipocytes</topic><topic>Adipose Tissue, Brown - cytology</topic><topic>Adipose Tissue, Brown - drug effects</topic><topic>Adipose Tissue, Brown - metabolism</topic><topic>Animals</topic><topic>brown adipose tissue</topic><topic>cafeterias</topic><topic>calorimeters</topic><topic>Calorimetry</topic><topic>Diet</topic><topic>Glucose - pharmacology</topic><topic>heat production</topic><topic>Hot Temperature</topic><topic>In Vitro Techniques</topic><topic>Male</topic><topic>Norepinephrine - pharmacology</topic><topic>Oleic Acid</topic><topic>Oleic Acids - pharmacology</topic><topic>oxygen consumption</topic><topic>Oxygen Consumption - drug effects</topic><topic>Propranolol - pharmacology</topic><topic>Rats</topic><topic>Rats, Inbred Strains</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Clark, D.G</creatorcontrib><creatorcontrib>Brinkman, M</creatorcontrib><creatorcontrib>Neville, S.D</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Biochemical journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Clark, D.G</au><au>Brinkman, M</au><au>Neville, S.D</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Microcalorimetric measurements of heat production in brown adipocytes from control and cafeteria-fed rats</atitle><jtitle>Biochemical journal</jtitle><addtitle>Biochem J</addtitle><date>1986-04-15</date><risdate>1986</risdate><volume>235</volume><issue>2</issue><spage>337</spage><epage>342</epage><pages>337-342</pages><issn>0264-6021</issn><eissn>1470-8728</eissn><abstract>The effects of the sequential addition of glucose, noradrenaline, propranolol and oleic acid on the rates of O2 consumption and heat production by isolated interscapular brown adipocytes from control and cafeteria-fed rats were compared. Although the chemical agents produced very similar changes in oxidative metabolism, the actual rates of O2 uptake and heat output in adipocytes from the cafeteria-fed rats, when expressed per g dry wt. of cells, were approx. 65% less than those obtained with cells from the control rats. However, when the same results were expressed per 10(8) multiloccular brown adipocytes, rather than gravimetrically, rates of O2 consumption and heat production were equivalent. Further interpretation of these data is complicated, because the average volume of multiloccular brown adipocytes from cafeteria-fed rats was 2.5 times that for multiloccular cells from control animals.</abstract><cop>England</cop><pmid>3741394</pmid><doi>10.1042/bj2350337</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0264-6021 |
| ispartof | Biochemical journal, 1986-04, Vol.235 (2), p.337-342 |
| issn | 0264-6021 1470-8728 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_1146692 |
| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; PubMed Central; Alma/SFX Local Collection |
| subjects | adipocytes Adipose Tissue, Brown - cytology Adipose Tissue, Brown - drug effects Adipose Tissue, Brown - metabolism Animals brown adipose tissue cafeterias calorimeters Calorimetry Diet Glucose - pharmacology heat production Hot Temperature In Vitro Techniques Male Norepinephrine - pharmacology Oleic Acid Oleic Acids - pharmacology oxygen consumption Oxygen Consumption - drug effects Propranolol - pharmacology Rats Rats, Inbred Strains |
| title | Microcalorimetric measurements of heat production in brown adipocytes from control and cafeteria-fed rats |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-29T03%3A06%3A32IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Microcalorimetric%20measurements%20of%20heat%20production%20in%20brown%20adipocytes%20from%20control%20and%20cafeteria-fed%20rats&rft.jtitle=Biochemical%20journal&rft.au=Clark,%20D.G&rft.date=1986-04-15&rft.volume=235&rft.issue=2&rft.spage=337&rft.epage=342&rft.pages=337-342&rft.issn=0264-6021&rft.eissn=1470-8728&rft_id=info:doi/10.1042/bj2350337&rft_dat=%3Cproquest_pubme%3E76988095%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=76988095&rft_id=info:pmid/3741394&rfr_iscdi=true |