Exercise training decreases the load and changes the content of circulating SDS-resistant protein aggregates in patients with heart failure with reduced ejection fraction
Background Heart failure (HF) often disrupts the protein quality control (PQC) system leading to protein aggregate accumulation. Evidence from tissue biopsies showed that exercise restores PQC system in HF; however, little is known about its effects on plasma proteostasis. Aim To determine the effec...
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| Veröffentlicht in: | Molecular and cellular biochemistry 2024-10, Vol.479 (10), p.2711-2722 |
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| creator | Gouveia, Marisol Schmidt, Cristine Basilio, Priscilla Gois Aveiro, Susana S. Domingues, Pedro Xia, Ke Colón, Wilfredo Vitorino, Rui Ferreira, Rita Santos, Mário Vieira, Sandra I. Ribeiro, Fernando |
| description | Background
Heart failure (HF) often disrupts the protein quality control (PQC) system leading to protein aggregate accumulation. Evidence from tissue biopsies showed that exercise restores PQC system in HF; however, little is known about its effects on plasma proteostasis.
Aim
To determine the effects of exercise training on the load and composition of plasma SDS-resistant protein aggregates (SRA) in patients with HF with reduced ejection fraction (HFrEF).
Methods
Eighteen patients with HFrEF (age: 63.4 ± 6.5 years; LVEF: 33.4 ± 11.6%) participated in a 12-week combined (aerobic plus resistance) exercise program (60 min/session, twice per week). The load and content of circulating SRA were assessed using D2D SDS-PAGE and mass spectrometry. Cardiorespiratory fitness, quality of life, and circulating levels of high-sensitive C-reactive protein, N-terminal pro-B-type natriuretic peptide (NT-proBNP), haptoglobin and ficolin-3, were also evaluated at baseline and after the exercise program.
Results
The exercise program decreased the plasma SRA load (% SRA/total protein: 38.0 ± 8.9 to 36.1 ± 9.7%, p = 0.018; % SRA/soluble fraction: 64.3 ± 27.1 to 59.8 ± 27.7%, p = 0.003). Plasma SRA of HFrEF patients comprised 31 proteins, with α-2-macroglobulin and haptoglobin as the most abundant ones. The exercise training significantly increased haptoglobin plasma levels (1.03 ± 0.40 to 1.11 ± 0.46, p = 0.031), while decreasing its abundance in SRA (1.83 ± 0.54 × 10
11
to 1.51 ± 0.59 × 10
11
, p = 0.049). Cardiorespiratory fitness [16.4(5.9) to 19.0(5.2) ml/kg/min, p = 0.002], quality of life, and circulating NT-proBNP [720.0(850.0) to 587.0(847.3) pg/mL, p = 0.048] levels, also improved after the exercise program.
Conclusion
Exercise training reduced the plasma SRA load and enhanced PQC, potentially via haptoglobin-mediated action, while improving cardiorespiratory fitness and quality of life of patients with HFrEF. |
| doi_str_mv | 10.1007/s11010-023-04884-z |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_11455743</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>3113185760</sourcerecordid><originalsourceid>FETCH-LOGICAL-c454t-564b12873122f2b89c8bf84393634b67b92278791152a359532cb9953cc3bee53</originalsourceid><addsrcrecordid>eNp9UsmO1DAQjRCIaQZ-gAOyxIVLwGtsnxAahkUaicPA2XKcStqttN3YDst8El-JZ9IMy4FT2VXvPVe5XtM8Jvg5wVi-yIRggltMWYu5Ury9utNsiJCs5Zrou80GM4xbRaQ8aR7kvMMVjQm535wwqTFVqts0P86_QXI-AyrJ-uDDhAZwCWyGjMoW0BztgGwYkNvaMB2TLoYCoaA4IueTW2ZbrpmXry_bBNnnYmvxkGIBH5CdpgSTLZVbb4cKrdSMvvqyRVuwqaDR-nlJsKYSDIuDAcEOXPExoDHZm8PD5t5o5wyPjvG0-fTm_OPZu_biw9v3Z68uWscFL63oeE-okoxQOtJeaaf6UXGmWcd438leUyqV1IQIapnQglHX6xqcYz2AYKfNy1X3sPR7GFztNtnZHJLf2_TdROvN35Xgt2aKXwwhXAjJWVV4dlRI8fMCuZi9zw7m2QaISzb163knJdWyQp_-A93FJYU6n2GEMKKE7HBF0RXlUsw5wXjbDcHm2gtm9YKpXjA3XjBXlfTkzzluKb-WXwFsBeRaqrtNv9_-j-xPiYDDQg</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>3113185760</pqid></control><display><type>article</type><title>Exercise training decreases the load and changes the content of circulating SDS-resistant protein aggregates in patients with heart failure with reduced ejection fraction</title><source>MEDLINE</source><source>Springer Nature - Complete Springer Journals</source><creator>Gouveia, Marisol ; Schmidt, Cristine ; Basilio, Priscilla Gois ; Aveiro, Susana S. ; Domingues, Pedro ; Xia, Ke ; Colón, Wilfredo ; Vitorino, Rui ; Ferreira, Rita ; Santos, Mário ; Vieira, Sandra I. ; Ribeiro, Fernando</creator><creatorcontrib>Gouveia, Marisol ; Schmidt, Cristine ; Basilio, Priscilla Gois ; Aveiro, Susana S. ; Domingues, Pedro ; Xia, Ke ; Colón, Wilfredo ; Vitorino, Rui ; Ferreira, Rita ; Santos, Mário ; Vieira, Sandra I. ; Ribeiro, Fernando</creatorcontrib><description>Background
Heart failure (HF) often disrupts the protein quality control (PQC) system leading to protein aggregate accumulation. Evidence from tissue biopsies showed that exercise restores PQC system in HF; however, little is known about its effects on plasma proteostasis.
Aim
To determine the effects of exercise training on the load and composition of plasma SDS-resistant protein aggregates (SRA) in patients with HF with reduced ejection fraction (HFrEF).
Methods
Eighteen patients with HFrEF (age: 63.4 ± 6.5 years; LVEF: 33.4 ± 11.6%) participated in a 12-week combined (aerobic plus resistance) exercise program (60 min/session, twice per week). The load and content of circulating SRA were assessed using D2D SDS-PAGE and mass spectrometry. Cardiorespiratory fitness, quality of life, and circulating levels of high-sensitive C-reactive protein, N-terminal pro-B-type natriuretic peptide (NT-proBNP), haptoglobin and ficolin-3, were also evaluated at baseline and after the exercise program.
Results
The exercise program decreased the plasma SRA load (% SRA/total protein: 38.0 ± 8.9 to 36.1 ± 9.7%, p = 0.018; % SRA/soluble fraction: 64.3 ± 27.1 to 59.8 ± 27.7%, p = 0.003). Plasma SRA of HFrEF patients comprised 31 proteins, with α-2-macroglobulin and haptoglobin as the most abundant ones. The exercise training significantly increased haptoglobin plasma levels (1.03 ± 0.40 to 1.11 ± 0.46, p = 0.031), while decreasing its abundance in SRA (1.83 ± 0.54 × 10
11
to 1.51 ± 0.59 × 10
11
, p = 0.049). Cardiorespiratory fitness [16.4(5.9) to 19.0(5.2) ml/kg/min, p = 0.002], quality of life, and circulating NT-proBNP [720.0(850.0) to 587.0(847.3) pg/mL, p = 0.048] levels, also improved after the exercise program.
Conclusion
Exercise training reduced the plasma SRA load and enhanced PQC, potentially via haptoglobin-mediated action, while improving cardiorespiratory fitness and quality of life of patients with HFrEF.</description><identifier>ISSN: 0300-8177</identifier><identifier>ISSN: 1573-4919</identifier><identifier>EISSN: 1573-4919</identifier><identifier>DOI: 10.1007/s11010-023-04884-z</identifier><identifier>PMID: 37902886</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Aged ; Aggregates ; Bioaccumulation ; Biochemistry ; Biomedical and Life Sciences ; Biopsy ; Brain natriuretic peptide ; C-reactive protein ; C-Reactive Protein - metabolism ; Cancer Research ; Cardiology ; Cardiorespiratory fitness ; Circulation ; Composition effects ; Congestive heart failure ; Ejection fraction ; Exercise ; Exercise - physiology ; Exercise Therapy - methods ; Female ; Fitness ; Fitness training programs ; Haptoglobin ; Heart failure ; Heart Failure - blood ; Heart Failure - physiopathology ; Heart Failure - therapy ; Humans ; Life Sciences ; Male ; Mass spectrometry ; Mass spectroscopy ; Medical Biochemistry ; Middle Aged ; Natriuretic Peptide, Brain - blood ; Peptide Fragments - blood ; Physical fitness ; Physical training ; Plasma ; Plasma levels ; Protein Aggregates ; Protein composition ; Proteins ; Quality control ; Quality of Life ; Stroke Volume</subject><ispartof>Molecular and cellular biochemistry, 2024-10, Vol.479 (10), p.2711-2722</ispartof><rights>The Author(s) 2023</rights><rights>2023. The Author(s).</rights><rights>The Author(s) 2023. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>The Author(s) 2023 2023</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c454t-564b12873122f2b89c8bf84393634b67b92278791152a359532cb9953cc3bee53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11010-023-04884-z$$EPDF$$P50$$Gspringer$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11010-023-04884-z$$EHTML$$P50$$Gspringer$$Hfree_for_read</linktohtml><link.rule.ids>230,314,776,780,881,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/37902886$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gouveia, Marisol</creatorcontrib><creatorcontrib>Schmidt, Cristine</creatorcontrib><creatorcontrib>Basilio, Priscilla Gois</creatorcontrib><creatorcontrib>Aveiro, Susana S.</creatorcontrib><creatorcontrib>Domingues, Pedro</creatorcontrib><creatorcontrib>Xia, Ke</creatorcontrib><creatorcontrib>Colón, Wilfredo</creatorcontrib><creatorcontrib>Vitorino, Rui</creatorcontrib><creatorcontrib>Ferreira, Rita</creatorcontrib><creatorcontrib>Santos, Mário</creatorcontrib><creatorcontrib>Vieira, Sandra I.</creatorcontrib><creatorcontrib>Ribeiro, Fernando</creatorcontrib><title>Exercise training decreases the load and changes the content of circulating SDS-resistant protein aggregates in patients with heart failure with reduced ejection fraction</title><title>Molecular and cellular biochemistry</title><addtitle>Mol Cell Biochem</addtitle><addtitle>Mol Cell Biochem</addtitle><description>Background
Heart failure (HF) often disrupts the protein quality control (PQC) system leading to protein aggregate accumulation. Evidence from tissue biopsies showed that exercise restores PQC system in HF; however, little is known about its effects on plasma proteostasis.
Aim
To determine the effects of exercise training on the load and composition of plasma SDS-resistant protein aggregates (SRA) in patients with HF with reduced ejection fraction (HFrEF).
Methods
Eighteen patients with HFrEF (age: 63.4 ± 6.5 years; LVEF: 33.4 ± 11.6%) participated in a 12-week combined (aerobic plus resistance) exercise program (60 min/session, twice per week). The load and content of circulating SRA were assessed using D2D SDS-PAGE and mass spectrometry. Cardiorespiratory fitness, quality of life, and circulating levels of high-sensitive C-reactive protein, N-terminal pro-B-type natriuretic peptide (NT-proBNP), haptoglobin and ficolin-3, were also evaluated at baseline and after the exercise program.
Results
The exercise program decreased the plasma SRA load (% SRA/total protein: 38.0 ± 8.9 to 36.1 ± 9.7%, p = 0.018; % SRA/soluble fraction: 64.3 ± 27.1 to 59.8 ± 27.7%, p = 0.003). Plasma SRA of HFrEF patients comprised 31 proteins, with α-2-macroglobulin and haptoglobin as the most abundant ones. The exercise training significantly increased haptoglobin plasma levels (1.03 ± 0.40 to 1.11 ± 0.46, p = 0.031), while decreasing its abundance in SRA (1.83 ± 0.54 × 10
11
to 1.51 ± 0.59 × 10
11
, p = 0.049). Cardiorespiratory fitness [16.4(5.9) to 19.0(5.2) ml/kg/min, p = 0.002], quality of life, and circulating NT-proBNP [720.0(850.0) to 587.0(847.3) pg/mL, p = 0.048] levels, also improved after the exercise program.
Conclusion
Exercise training reduced the plasma SRA load and enhanced PQC, potentially via haptoglobin-mediated action, while improving cardiorespiratory fitness and quality of life of patients with HFrEF.</description><subject>Aged</subject><subject>Aggregates</subject><subject>Bioaccumulation</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Biopsy</subject><subject>Brain natriuretic peptide</subject><subject>C-reactive protein</subject><subject>C-Reactive Protein - metabolism</subject><subject>Cancer Research</subject><subject>Cardiology</subject><subject>Cardiorespiratory fitness</subject><subject>Circulation</subject><subject>Composition effects</subject><subject>Congestive heart failure</subject><subject>Ejection fraction</subject><subject>Exercise</subject><subject>Exercise - physiology</subject><subject>Exercise Therapy - methods</subject><subject>Female</subject><subject>Fitness</subject><subject>Fitness training programs</subject><subject>Haptoglobin</subject><subject>Heart failure</subject><subject>Heart Failure - blood</subject><subject>Heart Failure - physiopathology</subject><subject>Heart Failure - therapy</subject><subject>Humans</subject><subject>Life Sciences</subject><subject>Male</subject><subject>Mass spectrometry</subject><subject>Mass spectroscopy</subject><subject>Medical Biochemistry</subject><subject>Middle Aged</subject><subject>Natriuretic Peptide, Brain - blood</subject><subject>Peptide Fragments - blood</subject><subject>Physical fitness</subject><subject>Physical training</subject><subject>Plasma</subject><subject>Plasma levels</subject><subject>Protein Aggregates</subject><subject>Protein composition</subject><subject>Proteins</subject><subject>Quality control</subject><subject>Quality of Life</subject><subject>Stroke Volume</subject><issn>0300-8177</issn><issn>1573-4919</issn><issn>1573-4919</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>EIF</sourceid><recordid>eNp9UsmO1DAQjRCIaQZ-gAOyxIVLwGtsnxAahkUaicPA2XKcStqttN3YDst8El-JZ9IMy4FT2VXvPVe5XtM8Jvg5wVi-yIRggltMWYu5Ury9utNsiJCs5Zrou80GM4xbRaQ8aR7kvMMVjQm535wwqTFVqts0P86_QXI-AyrJ-uDDhAZwCWyGjMoW0BztgGwYkNvaMB2TLoYCoaA4IueTW2ZbrpmXry_bBNnnYmvxkGIBH5CdpgSTLZVbb4cKrdSMvvqyRVuwqaDR-nlJsKYSDIuDAcEOXPExoDHZm8PD5t5o5wyPjvG0-fTm_OPZu_biw9v3Z68uWscFL63oeE-okoxQOtJeaaf6UXGmWcd438leUyqV1IQIapnQglHX6xqcYz2AYKfNy1X3sPR7GFztNtnZHJLf2_TdROvN35Xgt2aKXwwhXAjJWVV4dlRI8fMCuZi9zw7m2QaISzb163knJdWyQp_-A93FJYU6n2GEMKKE7HBF0RXlUsw5wXjbDcHm2gtm9YKpXjA3XjBXlfTkzzluKb-WXwFsBeRaqrtNv9_-j-xPiYDDQg</recordid><startdate>20241001</startdate><enddate>20241001</enddate><creator>Gouveia, Marisol</creator><creator>Schmidt, Cristine</creator><creator>Basilio, Priscilla Gois</creator><creator>Aveiro, Susana S.</creator><creator>Domingues, Pedro</creator><creator>Xia, Ke</creator><creator>Colón, Wilfredo</creator><creator>Vitorino, Rui</creator><creator>Ferreira, Rita</creator><creator>Santos, Mário</creator><creator>Vieira, Sandra I.</creator><creator>Ribeiro, Fernando</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>C6C</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QP</scope><scope>7T5</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20241001</creationdate><title>Exercise training decreases the load and changes the content of circulating SDS-resistant protein aggregates in patients with heart failure with reduced ejection fraction</title><author>Gouveia, Marisol ; Schmidt, Cristine ; Basilio, Priscilla Gois ; Aveiro, Susana S. ; Domingues, Pedro ; Xia, Ke ; Colón, Wilfredo ; Vitorino, Rui ; Ferreira, Rita ; Santos, Mário ; Vieira, Sandra I. ; Ribeiro, Fernando</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c454t-564b12873122f2b89c8bf84393634b67b92278791152a359532cb9953cc3bee53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Aged</topic><topic>Aggregates</topic><topic>Bioaccumulation</topic><topic>Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Biopsy</topic><topic>Brain natriuretic peptide</topic><topic>C-reactive protein</topic><topic>C-Reactive Protein - metabolism</topic><topic>Cancer Research</topic><topic>Cardiology</topic><topic>Cardiorespiratory fitness</topic><topic>Circulation</topic><topic>Composition effects</topic><topic>Congestive heart failure</topic><topic>Ejection fraction</topic><topic>Exercise</topic><topic>Exercise - physiology</topic><topic>Exercise Therapy - methods</topic><topic>Female</topic><topic>Fitness</topic><topic>Fitness training programs</topic><topic>Haptoglobin</topic><topic>Heart failure</topic><topic>Heart Failure - blood</topic><topic>Heart Failure - physiopathology</topic><topic>Heart Failure - therapy</topic><topic>Humans</topic><topic>Life Sciences</topic><topic>Male</topic><topic>Mass spectrometry</topic><topic>Mass spectroscopy</topic><topic>Medical Biochemistry</topic><topic>Middle Aged</topic><topic>Natriuretic Peptide, Brain - blood</topic><topic>Peptide Fragments - blood</topic><topic>Physical fitness</topic><topic>Physical training</topic><topic>Plasma</topic><topic>Plasma levels</topic><topic>Protein Aggregates</topic><topic>Protein composition</topic><topic>Proteins</topic><topic>Quality control</topic><topic>Quality of Life</topic><topic>Stroke Volume</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gouveia, Marisol</creatorcontrib><creatorcontrib>Schmidt, Cristine</creatorcontrib><creatorcontrib>Basilio, Priscilla Gois</creatorcontrib><creatorcontrib>Aveiro, Susana S.</creatorcontrib><creatorcontrib>Domingues, Pedro</creatorcontrib><creatorcontrib>Xia, Ke</creatorcontrib><creatorcontrib>Colón, Wilfredo</creatorcontrib><creatorcontrib>Vitorino, Rui</creatorcontrib><creatorcontrib>Ferreira, Rita</creatorcontrib><creatorcontrib>Santos, Mário</creatorcontrib><creatorcontrib>Vieira, Sandra I.</creatorcontrib><creatorcontrib>Ribeiro, Fernando</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Molecular and cellular biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gouveia, Marisol</au><au>Schmidt, Cristine</au><au>Basilio, Priscilla Gois</au><au>Aveiro, Susana S.</au><au>Domingues, Pedro</au><au>Xia, Ke</au><au>Colón, Wilfredo</au><au>Vitorino, Rui</au><au>Ferreira, Rita</au><au>Santos, Mário</au><au>Vieira, Sandra I.</au><au>Ribeiro, Fernando</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Exercise training decreases the load and changes the content of circulating SDS-resistant protein aggregates in patients with heart failure with reduced ejection fraction</atitle><jtitle>Molecular and cellular biochemistry</jtitle><stitle>Mol Cell Biochem</stitle><addtitle>Mol Cell Biochem</addtitle><date>2024-10-01</date><risdate>2024</risdate><volume>479</volume><issue>10</issue><spage>2711</spage><epage>2722</epage><pages>2711-2722</pages><issn>0300-8177</issn><issn>1573-4919</issn><eissn>1573-4919</eissn><abstract>Background
Heart failure (HF) often disrupts the protein quality control (PQC) system leading to protein aggregate accumulation. Evidence from tissue biopsies showed that exercise restores PQC system in HF; however, little is known about its effects on plasma proteostasis.
Aim
To determine the effects of exercise training on the load and composition of plasma SDS-resistant protein aggregates (SRA) in patients with HF with reduced ejection fraction (HFrEF).
Methods
Eighteen patients with HFrEF (age: 63.4 ± 6.5 years; LVEF: 33.4 ± 11.6%) participated in a 12-week combined (aerobic plus resistance) exercise program (60 min/session, twice per week). The load and content of circulating SRA were assessed using D2D SDS-PAGE and mass spectrometry. Cardiorespiratory fitness, quality of life, and circulating levels of high-sensitive C-reactive protein, N-terminal pro-B-type natriuretic peptide (NT-proBNP), haptoglobin and ficolin-3, were also evaluated at baseline and after the exercise program.
Results
The exercise program decreased the plasma SRA load (% SRA/total protein: 38.0 ± 8.9 to 36.1 ± 9.7%, p = 0.018; % SRA/soluble fraction: 64.3 ± 27.1 to 59.8 ± 27.7%, p = 0.003). Plasma SRA of HFrEF patients comprised 31 proteins, with α-2-macroglobulin and haptoglobin as the most abundant ones. The exercise training significantly increased haptoglobin plasma levels (1.03 ± 0.40 to 1.11 ± 0.46, p = 0.031), while decreasing its abundance in SRA (1.83 ± 0.54 × 10
11
to 1.51 ± 0.59 × 10
11
, p = 0.049). Cardiorespiratory fitness [16.4(5.9) to 19.0(5.2) ml/kg/min, p = 0.002], quality of life, and circulating NT-proBNP [720.0(850.0) to 587.0(847.3) pg/mL, p = 0.048] levels, also improved after the exercise program.
Conclusion
Exercise training reduced the plasma SRA load and enhanced PQC, potentially via haptoglobin-mediated action, while improving cardiorespiratory fitness and quality of life of patients with HFrEF.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>37902886</pmid><doi>10.1007/s11010-023-04884-z</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Molecular and cellular biochemistry, 2024-10, Vol.479 (10), p.2711-2722 |
| issn | 0300-8177 1573-4919 1573-4919 |
| language | eng |
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| source | MEDLINE; Springer Nature - Complete Springer Journals |
| subjects | Aged Aggregates Bioaccumulation Biochemistry Biomedical and Life Sciences Biopsy Brain natriuretic peptide C-reactive protein C-Reactive Protein - metabolism Cancer Research Cardiology Cardiorespiratory fitness Circulation Composition effects Congestive heart failure Ejection fraction Exercise Exercise - physiology Exercise Therapy - methods Female Fitness Fitness training programs Haptoglobin Heart failure Heart Failure - blood Heart Failure - physiopathology Heart Failure - therapy Humans Life Sciences Male Mass spectrometry Mass spectroscopy Medical Biochemistry Middle Aged Natriuretic Peptide, Brain - blood Peptide Fragments - blood Physical fitness Physical training Plasma Plasma levels Protein Aggregates Protein composition Proteins Quality control Quality of Life Stroke Volume |
| title | Exercise training decreases the load and changes the content of circulating SDS-resistant protein aggregates in patients with heart failure with reduced ejection fraction |
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