MSRE-PCR for analysis of gene-specific DNA methylation

MSRE-PCR for analysis of gene-specific DNA methylation

Abnormal DNA methylation is observed in certain promoters of neoplastic cells, although the likelihood of methylation for each individual promoter varies. Simultaneous analysis of many promoters in the same sample can allow use of statistical methods for identification of neoplasia. Here we describe...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Nucleic acids research 2005-01, Vol.33 (10), p.e93-e93
Hauptverfasser: Melnikov, Anatoliy A., Gartenhaus, Ronald B., Levenson, Anait S., Motchoulskaia, Natalia A., Levenson (Chernokhvostov), Victor V.
Format: Artikel
Sprache:eng
Schlagworte:
Breast Neoplasms - genetics
Cell Line, Tumor
DNA Methylation
DNA Restriction Enzymes - metabolism
Female
Humans
Methods Online
Neoplasms - genetics
Polymerase Chain Reaction - methods
Promoter Regions, Genetic
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:Abnormal DNA methylation is observed in certain promoters of neoplastic cells, although the likelihood of methylation for each individual promoter varies. Simultaneous analysis of many promoters in the same sample can allow use of statistical methods for identification of neoplasia. Here we describe an assay for such analysis, based on digestion of genomic DNA with methylation-sensitive restriction enzyme and multiplexed PCR with gene-specific primers (MSRE-PCR). MSRE-PCR includes extensive digestion of genomic DNA (uncut fragments cannot be identified by PCR), can be applied to dilute samples (
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/gni092