Muscarinic-receptor stimulation enhances polyphosphoinositide breakdown in guinea-pig ileum smooth muscle
Muscarinic-receptor stimulation by 0.1 mM-carbachol in longitudinal muscle of the guinea-pig ileum increases the incorporation of [3H]inositol into inositol-containing phospholipid. This effect was blocked by 16 microM-atropine. After 60 min incubation, carbachol increased the accumulation of total...
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| Veröffentlicht in: | Biochemical journal 1984-10, Vol.223 (2), p.527-531 |
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| creator | Sekar, M C Roufogalis, B D |
| description | Muscarinic-receptor stimulation by 0.1 mM-carbachol in longitudinal muscle of the guinea-pig ileum increases the incorporation of [3H]inositol into inositol-containing phospholipid. This effect was blocked by 16 microM-atropine. After 60 min incubation, carbachol increased the accumulation of total inositol phosphates 20-fold in the presence of 10 mM-Li+. Less than 20% of the total inositol phosphate corresponded to inositol 1-phosphate by ion-exchange chromatography, whereas of the remainder about two-thirds corresponded to inositol bisphosphate and one third to inositol trisphosphate. It is concluded that stimulation of muscarinic receptors in guinea-pig ileum enhances breakdown of polyphosphoinositides, suggesting that this may be a primary event associated with Ca2+ mobilization in the guinea-pig ileum. |
| doi_str_mv | 10.1042/bj2230527 |
| format | Article |
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This effect was blocked by 16 microM-atropine. After 60 min incubation, carbachol increased the accumulation of total inositol phosphates 20-fold in the presence of 10 mM-Li+. Less than 20% of the total inositol phosphate corresponded to inositol 1-phosphate by ion-exchange chromatography, whereas of the remainder about two-thirds corresponded to inositol bisphosphate and one third to inositol trisphosphate. It is concluded that stimulation of muscarinic receptors in guinea-pig ileum enhances breakdown of polyphosphoinositides, suggesting that this may be a primary event associated with Ca2+ mobilization in the guinea-pig ileum.</description><identifier>ISSN: 0264-6021</identifier><identifier>EISSN: 1470-8728</identifier><identifier>DOI: 10.1042/bj2230527</identifier><identifier>PMID: 6093776</identifier><language>eng</language><publisher>England</publisher><subject>acetylcholine ; Animals ; Atropine - pharmacology ; Carbachol - pharmacology ; Female ; Guinea Pigs ; ileum ; In Vitro Techniques ; Inositol - metabolism ; Lithium - pharmacology ; Muscle, Smooth - metabolism ; Phosphatidylinositol Phosphates ; Phosphatidylinositols - metabolism ; Phospholipids - metabolism ; polyphosphoinositides ; Receptors, Muscarinic - drug effects ; Receptors, Muscarinic - metabolism ; smooth muscle ; Stimulation, Chemical</subject><ispartof>Biochemical journal, 1984-10, Vol.223 (2), p.527-531</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c401t-141f77d433bddba87ac408fde71bd17d4982ee83e7a6f856b283033dfc384d8c3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1144327/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1144327/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6093776$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sekar, M C</creatorcontrib><creatorcontrib>Roufogalis, B D</creatorcontrib><title>Muscarinic-receptor stimulation enhances polyphosphoinositide breakdown in guinea-pig ileum smooth muscle</title><title>Biochemical journal</title><addtitle>Biochem J</addtitle><description>Muscarinic-receptor stimulation by 0.1 mM-carbachol in longitudinal muscle of the guinea-pig ileum increases the incorporation of [3H]inositol into inositol-containing phospholipid. This effect was blocked by 16 microM-atropine. After 60 min incubation, carbachol increased the accumulation of total inositol phosphates 20-fold in the presence of 10 mM-Li+. Less than 20% of the total inositol phosphate corresponded to inositol 1-phosphate by ion-exchange chromatography, whereas of the remainder about two-thirds corresponded to inositol bisphosphate and one third to inositol trisphosphate. It is concluded that stimulation of muscarinic receptors in guinea-pig ileum enhances breakdown of polyphosphoinositides, suggesting that this may be a primary event associated with Ca2+ mobilization in the guinea-pig ileum.</description><subject>acetylcholine</subject><subject>Animals</subject><subject>Atropine - pharmacology</subject><subject>Carbachol - pharmacology</subject><subject>Female</subject><subject>Guinea Pigs</subject><subject>ileum</subject><subject>In Vitro Techniques</subject><subject>Inositol - metabolism</subject><subject>Lithium - pharmacology</subject><subject>Muscle, Smooth - metabolism</subject><subject>Phosphatidylinositol Phosphates</subject><subject>Phosphatidylinositols - metabolism</subject><subject>Phospholipids - metabolism</subject><subject>polyphosphoinositides</subject><subject>Receptors, Muscarinic - drug effects</subject><subject>Receptors, Muscarinic - metabolism</subject><subject>smooth muscle</subject><subject>Stimulation, Chemical</subject><issn>0264-6021</issn><issn>1470-8728</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1984</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU1LxDAQhoMo6_px8AcIOQkeqvnaJl4EEb9A8aLnkCbT3Wib1KRV9t9b2WXRm4dhYObhnZd5ETqi5IwSwc6rN8Y4mTG5haZUSFIoydQ2mhJWiqIkjO6ivZzfCKGCCDJBk5JccCnLKfJPQ7Ym-eBtkcBC18eEc-_boTG9jwFDWJhgIeMuNstuEfNYPsTse-8AVwnMu4tfAfuA54MPYIrOz7FvYGhxbmPsF7gdTzRwgHZq02Q4XPd99Hp783J9Xzw-3z1cXz0WVhDaF1TQWkonOK-cq4ySZpyr2oGklaPj4kIxAMVBmrJWs7JiihPOXW25Ek5Zvo8uV7rdULXgLIQ-mUZ3ybcmLXU0Xv_dBL_Q8_ipKRWCMzkKnKwFUvwYIPe69dlC05gAcchaUc5m5B_g-GxGZ4yP4OkKtCnmnKDeuKFE_wSoNwGO7PFv-xtynRj_BkAymfs</recordid><startdate>19841015</startdate><enddate>19841015</enddate><creator>Sekar, M C</creator><creator>Roufogalis, B D</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>8FD</scope><scope>FR3</scope><scope>M7Z</scope><scope>P64</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19841015</creationdate><title>Muscarinic-receptor stimulation enhances polyphosphoinositide breakdown in guinea-pig ileum smooth muscle</title><author>Sekar, M C ; Roufogalis, B D</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c401t-141f77d433bddba87ac408fde71bd17d4982ee83e7a6f856b283033dfc384d8c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1984</creationdate><topic>acetylcholine</topic><topic>Animals</topic><topic>Atropine - pharmacology</topic><topic>Carbachol - pharmacology</topic><topic>Female</topic><topic>Guinea Pigs</topic><topic>ileum</topic><topic>In Vitro Techniques</topic><topic>Inositol - metabolism</topic><topic>Lithium - pharmacology</topic><topic>Muscle, Smooth - metabolism</topic><topic>Phosphatidylinositol Phosphates</topic><topic>Phosphatidylinositols - metabolism</topic><topic>Phospholipids - metabolism</topic><topic>polyphosphoinositides</topic><topic>Receptors, Muscarinic - drug effects</topic><topic>Receptors, Muscarinic - metabolism</topic><topic>smooth muscle</topic><topic>Stimulation, Chemical</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sekar, M C</creatorcontrib><creatorcontrib>Roufogalis, B D</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biochemistry Abstracts 1</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Biochemical journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sekar, M C</au><au>Roufogalis, B D</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Muscarinic-receptor stimulation enhances polyphosphoinositide breakdown in guinea-pig ileum smooth muscle</atitle><jtitle>Biochemical journal</jtitle><addtitle>Biochem J</addtitle><date>1984-10-15</date><risdate>1984</risdate><volume>223</volume><issue>2</issue><spage>527</spage><epage>531</epage><pages>527-531</pages><issn>0264-6021</issn><eissn>1470-8728</eissn><abstract>Muscarinic-receptor stimulation by 0.1 mM-carbachol in longitudinal muscle of the guinea-pig ileum increases the incorporation of [3H]inositol into inositol-containing phospholipid. This effect was blocked by 16 microM-atropine. After 60 min incubation, carbachol increased the accumulation of total inositol phosphates 20-fold in the presence of 10 mM-Li+. Less than 20% of the total inositol phosphate corresponded to inositol 1-phosphate by ion-exchange chromatography, whereas of the remainder about two-thirds corresponded to inositol bisphosphate and one third to inositol trisphosphate. It is concluded that stimulation of muscarinic receptors in guinea-pig ileum enhances breakdown of polyphosphoinositides, suggesting that this may be a primary event associated with Ca2+ mobilization in the guinea-pig ileum.</abstract><cop>England</cop><pmid>6093776</pmid><doi>10.1042/bj2230527</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Biochemical journal, 1984-10, Vol.223 (2), p.527-531 |
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| language | eng |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Alma/SFX Local Collection |
| subjects | acetylcholine Animals Atropine - pharmacology Carbachol - pharmacology Female Guinea Pigs ileum In Vitro Techniques Inositol - metabolism Lithium - pharmacology Muscle, Smooth - metabolism Phosphatidylinositol Phosphates Phosphatidylinositols - metabolism Phospholipids - metabolism polyphosphoinositides Receptors, Muscarinic - drug effects Receptors, Muscarinic - metabolism smooth muscle Stimulation, Chemical |
| title | Muscarinic-receptor stimulation enhances polyphosphoinositide breakdown in guinea-pig ileum smooth muscle |
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