Whole-cell recordings of inwardly rectifying K+ currents activated by 5-HT1A receptors on dorsal raphe neurones of the adult rat
1. An inwardly rectifying K+ current activated by serotonin (5-HT) was recorded from acutely isolated adult dorsal raphe (DR) neurones using the whole-cell recording mode of the patch clamp technique. 2. The 5-HT-induced K+ current (I5-HT) was only visible at an [K+]0 > 5 mM and it was observed i...
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| Veröffentlicht in: | The Journal of physiology 1993-09, Vol.469 (1), p.387-405 |
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| description | 1. An inwardly rectifying K+ current activated by serotonin (5-HT) was recorded from acutely isolated adult dorsal raphe (DR)
neurones using the whole-cell recording mode of the patch clamp technique. 2. The 5-HT-induced K+ current (I5-HT) was only
visible at an [K+]0 > 5 mM and it was observed in 69% of the cells. 3. The reversal potential for I5-HT was close to the potassium
equilibrium potential and was shifted by 51 mV per 10-fold change in [K+]0 indicating that I5-HT was carried predominantly
by K+. The chord conductance of I5-HT at -90 mV was proportional to the external [K+] raised to a fractional power. 4. A dose-response
relationship revealed that I5-HT was activated with an ED50 of 30 nM. Ba2+ (0.1 mM) blocked I5-HT completely. Spiperone reversibly
antagonized the response to 5-HT and 8-OHDPAT (8-hydroxy-2-(di-n-propylamino)tetralin) mimicked the response indicating that
the receptor activated was of the 5-HT1A subtype. 5. The response to 5-HT was largely prevented by in vitro pretreatment of
the cells with pertussis toxin (PTX) indicating the involvement of a PTX-sensitive G-protein in the transduction mechanism.
6. cAMP and lipoxygenase metabolites, both implicated in the modulation of similar currents in other preparations, were found
not to alter the effectiveness of 5-HT. 7. Glibenclamide and tolbutamide, blockers of the ATP-regulated K+ channel, did not
reduce the effect of 5-HT in DR neurones. 8. These results show that in acutely isolated adult DR neurones 5-HT activates
an inwardly rectifying K+ current and this involves a PTX-sensitive G-protein in the transduction pathway which may interact
with the K+ channel directly. |
| doi_str_mv | 10.1113/jphysiol.1993.sp019819 |
| format | Article |
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neurones using the whole-cell recording mode of the patch clamp technique. 2. The 5-HT-induced K+ current (I5-HT) was only
visible at an [K+]0 > 5 mM and it was observed in 69% of the cells. 3. The reversal potential for I5-HT was close to the potassium
equilibrium potential and was shifted by 51 mV per 10-fold change in [K+]0 indicating that I5-HT was carried predominantly
by K+. The chord conductance of I5-HT at -90 mV was proportional to the external [K+] raised to a fractional power. 4. A dose-response
relationship revealed that I5-HT was activated with an ED50 of 30 nM. Ba2+ (0.1 mM) blocked I5-HT completely. Spiperone reversibly
antagonized the response to 5-HT and 8-OHDPAT (8-hydroxy-2-(di-n-propylamino)tetralin) mimicked the response indicating that
the receptor activated was of the 5-HT1A subtype. 5. The response to 5-HT was largely prevented by in vitro pretreatment of
the cells with pertussis toxin (PTX) indicating the involvement of a PTX-sensitive G-protein in the transduction mechanism.
6. cAMP and lipoxygenase metabolites, both implicated in the modulation of similar currents in other preparations, were found
not to alter the effectiveness of 5-HT. 7. Glibenclamide and tolbutamide, blockers of the ATP-regulated K+ channel, did not
reduce the effect of 5-HT in DR neurones. 8. These results show that in acutely isolated adult DR neurones 5-HT activates
an inwardly rectifying K+ current and this involves a PTX-sensitive G-protein in the transduction pathway which may interact
with the K+ channel directly.</description><identifier>ISSN: 0022-3751</identifier><identifier>EISSN: 1469-7793</identifier><identifier>DOI: 10.1113/jphysiol.1993.sp019819</identifier><identifier>PMID: 8271204</identifier><identifier>CODEN: JPHYA7</identifier><language>eng</language><publisher>Oxford: The Physiological Society</publisher><subject>8-Hydroxy-2-(di-n-propylamino)tetralin - pharmacology ; Animals ; Barium - pharmacology ; Biological and medical sciences ; Central nervous system ; Central neurotransmission. Neuromudulation. Pathways and receptors ; Cyclic AMP - pharmacology ; Electrophysiology ; Fundamental and applied biological sciences. Psychology ; Glyburide - pharmacology ; In Vitro Techniques ; Ion Channel Gating - drug effects ; Masoprocol - pharmacology ; Membrane Potentials - drug effects ; Neurons - drug effects ; Neurons - metabolism ; Pertussis Toxin ; Potassium - pharmacology ; Potassium Channels - drug effects ; Raphe Nuclei - cytology ; Raphe Nuclei - drug effects ; Raphe Nuclei - metabolism ; Rats ; Receptors, Serotonin - drug effects ; Receptors, Serotonin - metabolism ; Serotonin - metabolism ; Serotonin - physiology ; Serotonin Antagonists - pharmacology ; Spiperone - pharmacology ; Tolbutamide - pharmacology ; Vertebrates: nervous system and sense organs ; Virulence Factors, Bordetella - pharmacology</subject><ispartof>The Journal of physiology, 1993-09, Vol.469 (1), p.387-405</ispartof><rights>1993 The Physiological Society</rights><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5437-f731410318a0961d4b0dd9d9904b07d57c499d344d55f09ca9a7d8d23286ce1b3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1143876/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1143876/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,724,777,781,882,1412,27905,27906,45555,45556,53772,53774</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4881195$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8271204$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Penington, N J</creatorcontrib><creatorcontrib>Kelly, J S</creatorcontrib><creatorcontrib>Fox, A P</creatorcontrib><title>Whole-cell recordings of inwardly rectifying K+ currents activated by 5-HT1A receptors on dorsal raphe neurones of the adult rat</title><title>The Journal of physiology</title><addtitle>J Physiol</addtitle><description>1. An inwardly rectifying K+ current activated by serotonin (5-HT) was recorded from acutely isolated adult dorsal raphe (DR)
neurones using the whole-cell recording mode of the patch clamp technique. 2. The 5-HT-induced K+ current (I5-HT) was only
visible at an [K+]0 > 5 mM and it was observed in 69% of the cells. 3. The reversal potential for I5-HT was close to the potassium
equilibrium potential and was shifted by 51 mV per 10-fold change in [K+]0 indicating that I5-HT was carried predominantly
by K+. The chord conductance of I5-HT at -90 mV was proportional to the external [K+] raised to a fractional power. 4. A dose-response
relationship revealed that I5-HT was activated with an ED50 of 30 nM. Ba2+ (0.1 mM) blocked I5-HT completely. Spiperone reversibly
antagonized the response to 5-HT and 8-OHDPAT (8-hydroxy-2-(di-n-propylamino)tetralin) mimicked the response indicating that
the receptor activated was of the 5-HT1A subtype. 5. The response to 5-HT was largely prevented by in vitro pretreatment of
the cells with pertussis toxin (PTX) indicating the involvement of a PTX-sensitive G-protein in the transduction mechanism.
6. cAMP and lipoxygenase metabolites, both implicated in the modulation of similar currents in other preparations, were found
not to alter the effectiveness of 5-HT. 7. Glibenclamide and tolbutamide, blockers of the ATP-regulated K+ channel, did not
reduce the effect of 5-HT in DR neurones. 8. These results show that in acutely isolated adult DR neurones 5-HT activates
an inwardly rectifying K+ current and this involves a PTX-sensitive G-protein in the transduction pathway which may interact
with the K+ channel directly.</description><subject>8-Hydroxy-2-(di-n-propylamino)tetralin - pharmacology</subject><subject>Animals</subject><subject>Barium - pharmacology</subject><subject>Biological and medical sciences</subject><subject>Central nervous system</subject><subject>Central neurotransmission. Neuromudulation. Pathways and receptors</subject><subject>Cyclic AMP - pharmacology</subject><subject>Electrophysiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glyburide - pharmacology</subject><subject>In Vitro Techniques</subject><subject>Ion Channel Gating - drug effects</subject><subject>Masoprocol - pharmacology</subject><subject>Membrane Potentials - drug effects</subject><subject>Neurons - drug effects</subject><subject>Neurons - metabolism</subject><subject>Pertussis Toxin</subject><subject>Potassium - pharmacology</subject><subject>Potassium Channels - drug effects</subject><subject>Raphe Nuclei - cytology</subject><subject>Raphe Nuclei - drug effects</subject><subject>Raphe Nuclei - metabolism</subject><subject>Rats</subject><subject>Receptors, Serotonin - drug effects</subject><subject>Receptors, Serotonin - metabolism</subject><subject>Serotonin - metabolism</subject><subject>Serotonin - physiology</subject><subject>Serotonin Antagonists - pharmacology</subject><subject>Spiperone - pharmacology</subject><subject>Tolbutamide - pharmacology</subject><subject>Vertebrates: nervous system and sense organs</subject><subject>Virulence Factors, Bordetella - pharmacology</subject><issn>0022-3751</issn><issn>1469-7793</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNksFu1DAQhiMEKtvCI4ByQICEsnjiJI4vSKUCClSCwyKOltd2Nq68cWonXeXWR2fCbldwQZxsz3zzjz2_k-Q5kCUA0LfXfTtF690SOKfL2BPgNfAHyQKKimeMcfowWRCS5xllJTxOTmO8JgQo4fwkOalzBjkpFsndz9Y7kynjXBqM8kHbbhNT36S228mg3TSHB9tMGE-_vknVGILphphKjN7Kweh0PaVldrmC8xk1_eADCnSpxlWiquxbk3ZmDL4zv5UHPEs9ugFzw5PkUSNdNE8P61ny4-OH1cVldvXt0-eL86tMlQVlWcMoFEAo1JLwCnSxJlpzzTnBHdMlUwXnmhaFLsuGcCW5ZLrWOc3rShlY07Pk3V63H9dboxW-IUgn-mC3MkzCSyv-znS2FRt_KwAKWrMKBV4eBIK_GU0cxNbGeW6yM36MglVAcao1gq__CQIraQloRY5otUdV8DEG0xzvA0TMNot7m8Vss7i3GQuf_fmaY9nBV8y_OORlVNI1QXbKxiNW1DUALxF7v8d21pnpP5uL1ZfvcwD_GeBkUOTVXqS1m3ZngxH7suiVNQM2q7gAMZO_ACWM18s</recordid><startdate>19930901</startdate><enddate>19930901</enddate><creator>Penington, N J</creator><creator>Kelly, J S</creator><creator>Fox, A P</creator><general>The Physiological Society</general><general>Blackwell</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19930901</creationdate><title>Whole-cell recordings of inwardly rectifying K+ currents activated by 5-HT1A receptors on dorsal raphe neurones of the adult rat</title><author>Penington, N J ; Kelly, J S ; Fox, A P</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5437-f731410318a0961d4b0dd9d9904b07d57c499d344d55f09ca9a7d8d23286ce1b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>8-Hydroxy-2-(di-n-propylamino)tetralin - pharmacology</topic><topic>Animals</topic><topic>Barium - pharmacology</topic><topic>Biological and medical sciences</topic><topic>Central nervous system</topic><topic>Central neurotransmission. Neuromudulation. Pathways and receptors</topic><topic>Cyclic AMP - pharmacology</topic><topic>Electrophysiology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Glyburide - pharmacology</topic><topic>In Vitro Techniques</topic><topic>Ion Channel Gating - drug effects</topic><topic>Masoprocol - pharmacology</topic><topic>Membrane Potentials - drug effects</topic><topic>Neurons - drug effects</topic><topic>Neurons - metabolism</topic><topic>Pertussis Toxin</topic><topic>Potassium - pharmacology</topic><topic>Potassium Channels - drug effects</topic><topic>Raphe Nuclei - cytology</topic><topic>Raphe Nuclei - drug effects</topic><topic>Raphe Nuclei - metabolism</topic><topic>Rats</topic><topic>Receptors, Serotonin - drug effects</topic><topic>Receptors, Serotonin - metabolism</topic><topic>Serotonin - metabolism</topic><topic>Serotonin - physiology</topic><topic>Serotonin Antagonists - pharmacology</topic><topic>Spiperone - pharmacology</topic><topic>Tolbutamide - pharmacology</topic><topic>Vertebrates: nervous system and sense organs</topic><topic>Virulence Factors, Bordetella - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Penington, N J</creatorcontrib><creatorcontrib>Kelly, J S</creatorcontrib><creatorcontrib>Fox, A P</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The Journal of physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Penington, N J</au><au>Kelly, J S</au><au>Fox, A P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Whole-cell recordings of inwardly rectifying K+ currents activated by 5-HT1A receptors on dorsal raphe neurones of the adult rat</atitle><jtitle>The Journal of physiology</jtitle><addtitle>J Physiol</addtitle><date>1993-09-01</date><risdate>1993</risdate><volume>469</volume><issue>1</issue><spage>387</spage><epage>405</epage><pages>387-405</pages><issn>0022-3751</issn><eissn>1469-7793</eissn><coden>JPHYA7</coden><abstract>1. An inwardly rectifying K+ current activated by serotonin (5-HT) was recorded from acutely isolated adult dorsal raphe (DR)
neurones using the whole-cell recording mode of the patch clamp technique. 2. The 5-HT-induced K+ current (I5-HT) was only
visible at an [K+]0 > 5 mM and it was observed in 69% of the cells. 3. The reversal potential for I5-HT was close to the potassium
equilibrium potential and was shifted by 51 mV per 10-fold change in [K+]0 indicating that I5-HT was carried predominantly
by K+. The chord conductance of I5-HT at -90 mV was proportional to the external [K+] raised to a fractional power. 4. A dose-response
relationship revealed that I5-HT was activated with an ED50 of 30 nM. Ba2+ (0.1 mM) blocked I5-HT completely. Spiperone reversibly
antagonized the response to 5-HT and 8-OHDPAT (8-hydroxy-2-(di-n-propylamino)tetralin) mimicked the response indicating that
the receptor activated was of the 5-HT1A subtype. 5. The response to 5-HT was largely prevented by in vitro pretreatment of
the cells with pertussis toxin (PTX) indicating the involvement of a PTX-sensitive G-protein in the transduction mechanism.
6. cAMP and lipoxygenase metabolites, both implicated in the modulation of similar currents in other preparations, were found
not to alter the effectiveness of 5-HT. 7. Glibenclamide and tolbutamide, blockers of the ATP-regulated K+ channel, did not
reduce the effect of 5-HT in DR neurones. 8. These results show that in acutely isolated adult DR neurones 5-HT activates
an inwardly rectifying K+ current and this involves a PTX-sensitive G-protein in the transduction pathway which may interact
with the K+ channel directly.</abstract><cop>Oxford</cop><pub>The Physiological Society</pub><pmid>8271204</pmid><doi>10.1113/jphysiol.1993.sp019819</doi><tpages>19</tpages><oa>free_for_read</oa></addata></record> |
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| language | eng |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete; IngentaConnect Free/Open Access Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central; Alma/SFX Local Collection |
| subjects | 8-Hydroxy-2-(di-n-propylamino)tetralin - pharmacology Animals Barium - pharmacology Biological and medical sciences Central nervous system Central neurotransmission. Neuromudulation. Pathways and receptors Cyclic AMP - pharmacology Electrophysiology Fundamental and applied biological sciences. Psychology Glyburide - pharmacology In Vitro Techniques Ion Channel Gating - drug effects Masoprocol - pharmacology Membrane Potentials - drug effects Neurons - drug effects Neurons - metabolism Pertussis Toxin Potassium - pharmacology Potassium Channels - drug effects Raphe Nuclei - cytology Raphe Nuclei - drug effects Raphe Nuclei - metabolism Rats Receptors, Serotonin - drug effects Receptors, Serotonin - metabolism Serotonin - metabolism Serotonin - physiology Serotonin Antagonists - pharmacology Spiperone - pharmacology Tolbutamide - pharmacology Vertebrates: nervous system and sense organs Virulence Factors, Bordetella - pharmacology |
| title | Whole-cell recordings of inwardly rectifying K+ currents activated by 5-HT1A receptors on dorsal raphe neurones of the adult rat |
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