Role of curvature and phase transition in lipid sorting and fission of membrane tubules
We have recently developed a minimal system for generating long tubular nanostructures that resemble tubes observed in vivo with biological membranes. Here, we studied membrane tube pulling in ternary mixtures of sphingomyelin, phosphatidylcholine and cholesterol. Two salient results emerged: the li...
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| Veröffentlicht in: | The EMBO journal 2005-04, Vol.24 (8), p.1537-1545 |
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| creator | Roux, Aurélien Cuvelier, Damien Nassoy, Pierre Prost, Jacques Bassereau, Patricia Goud, Bruno |
| description | We have recently developed a minimal system for generating long tubular nanostructures that resemble tubes observed
in vivo
with biological membranes. Here, we studied membrane tube pulling in ternary mixtures of sphingomyelin, phosphatidylcholine and cholesterol. Two salient results emerged: the lipid composition is significantly different in the tubes and in the vesicles; tube fission is observed when phase separation is generated in the tubes. This shows that lipid sorting may depend critically on both membrane curvature and phase separation. Phase separation also appears to be important for membrane fission in tubes pulled out of giant liposomes or purified Golgi membranes. |
| doi_str_mv | 10.1038/sj.emboj.7600631 |
| format | Article |
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in vivo
with biological membranes. Here, we studied membrane tube pulling in ternary mixtures of sphingomyelin, phosphatidylcholine and cholesterol. Two salient results emerged: the lipid composition is significantly different in the tubes and in the vesicles; tube fission is observed when phase separation is generated in the tubes. This shows that lipid sorting may depend critically on both membrane curvature and phase separation. Phase separation also appears to be important for membrane fission in tubes pulled out of giant liposomes or purified Golgi membranes.</description><identifier>ISSN: 0261-4189</identifier><identifier>EISSN: 1460-2075</identifier><identifier>DOI: 10.1038/sj.emboj.7600631</identifier><identifier>PMID: 15791208</identifier><identifier>CODEN: EMJODG</identifier><language>eng</language><publisher>Chichester, UK: John Wiley & Sons, Ltd</publisher><subject>Biological membranes ; Brain Chemistry ; Cell Membrane - chemistry ; Cell Membrane - metabolism ; Cholesterol ; Cholesterol - chemistry ; Cholesterol - metabolism ; Elasticity ; EMBO20 ; Golgi Apparatus - chemistry ; Lipid Bilayers - chemistry ; lipid sorting ; membrane curvature ; membrane fission ; membrane tubules ; phase separation ; Phosphatidylcholines - chemistry ; Phosphatidylcholines - metabolism ; Photochemistry ; Sphingomyelins - chemistry ; Sphingomyelins - metabolism ; Stress, Mechanical</subject><ispartof>The EMBO journal, 2005-04, Vol.24 (8), p.1537-1545</ispartof><rights>European Molecular Biology Organization 2005</rights><rights>Copyright © 2005 European Molecular Biology Organization</rights><rights>Copyright Nature Publishing Group Apr 20, 2005</rights><rights>Copyright © 2005, European Molecular Biology Organization 2005</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c6171-342fa8a197e3d1b26030083290e21d2302d3449782233ce12b860f621d102fe83</citedby><cites>FETCH-LOGICAL-c6171-342fa8a197e3d1b26030083290e21d2302d3449782233ce12b860f621d102fe83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1142567/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1142567/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,1411,1427,27901,27902,41096,42165,45550,45551,46384,46808,51551,53766,53768</link.rule.ids><linktorsrc>$$Uhttps://doi.org/10.1038/sj.emboj.7600631$$EView_record_in_Springer_Nature$$FView_record_in_$$GSpringer_Nature</linktorsrc><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15791208$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Roux, Aurélien</creatorcontrib><creatorcontrib>Cuvelier, Damien</creatorcontrib><creatorcontrib>Nassoy, Pierre</creatorcontrib><creatorcontrib>Prost, Jacques</creatorcontrib><creatorcontrib>Bassereau, Patricia</creatorcontrib><creatorcontrib>Goud, Bruno</creatorcontrib><title>Role of curvature and phase transition in lipid sorting and fission of membrane tubules</title><title>The EMBO journal</title><addtitle>EMBO J</addtitle><addtitle>EMBO J</addtitle><description>We have recently developed a minimal system for generating long tubular nanostructures that resemble tubes observed
in vivo
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Phase separation also appears to be important for membrane fission in tubes pulled out of giant liposomes or purified Golgi membranes.</description><subject>Biological membranes</subject><subject>Brain Chemistry</subject><subject>Cell Membrane - chemistry</subject><subject>Cell Membrane - metabolism</subject><subject>Cholesterol</subject><subject>Cholesterol - chemistry</subject><subject>Cholesterol - metabolism</subject><subject>Elasticity</subject><subject>EMBO20</subject><subject>Golgi Apparatus - chemistry</subject><subject>Lipid Bilayers - chemistry</subject><subject>lipid sorting</subject><subject>membrane curvature</subject><subject>membrane fission</subject><subject>membrane tubules</subject><subject>phase separation</subject><subject>Phosphatidylcholines - chemistry</subject><subject>Phosphatidylcholines - metabolism</subject><subject>Photochemistry</subject><subject>Sphingomyelins - chemistry</subject><subject>Sphingomyelins - metabolism</subject><subject>Stress, Mechanical</subject><issn>0261-4189</issn><issn>1460-2075</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqFkc1v1DAQxS0EokvhzglFHLhlmbET27kg0aofQLtICNSj5STO1iGJt3ZS6H9fb7NqCxLi5MO83_ObeYS8RlgiMPk-tEvTl65dCg7AGT4hC8w4pBRE_pQsgHJMM5TFHnkRQgsAuRT4nOxhLgqkIBfk4pvrTOKapJr8tR4nbxI91MnmUgeTjF4PwY7WDYkdks5ubJ0E50c7rO9UjQ1hO4x4H3NEdWSmcupMeEmeNboL5tXu3Sc_jo--H56mZ19PPh1-PEsrjgJTltFGS42FMKzGknJgAJLRAgzFmjKgNcuyQkhKGasM0lJyaHicIdDGSLZPPsy-m6nsTV2ZIYbu1MbbXvsb5bRVf04Ge6nW7lohZjTnIhq82xl4dzWZMKrehsp0XVzGTUFxIYtc5lvh27-ErZv8EJdTWOTx0gXfxoFZVHkXgjfNfRIEta1MhVbdVaZ2lUXkzeMNHoBdR1FQzIJftjM3_zVUR-cHnx_McWZDxIa18Y9C_ztQOjM2jOb3_X_a_4zHYCJXF6sT9UXSc7o6PlArdguApsTs</recordid><startdate>20050420</startdate><enddate>20050420</enddate><creator>Roux, Aurélien</creator><creator>Cuvelier, Damien</creator><creator>Nassoy, Pierre</creator><creator>Prost, Jacques</creator><creator>Bassereau, Patricia</creator><creator>Goud, Bruno</creator><general>John Wiley & Sons, Ltd</general><general>Nature Publishing Group UK</general><general>Springer Nature B.V</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QP</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>BKSAR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M7N</scope><scope>M7P</scope><scope>MBDVC</scope><scope>P64</scope><scope>PCBAR</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20050420</creationdate><title>Role of curvature and phase transition in lipid sorting and fission of membrane tubules</title><author>Roux, Aurélien ; Cuvelier, Damien ; Nassoy, Pierre ; Prost, Jacques ; Bassereau, Patricia ; Goud, Bruno</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c6171-342fa8a197e3d1b26030083290e21d2302d3449782233ce12b860f621d102fe83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Biological membranes</topic><topic>Brain Chemistry</topic><topic>Cell Membrane - 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| subjects | Biological membranes Brain Chemistry Cell Membrane - chemistry Cell Membrane - metabolism Cholesterol Cholesterol - chemistry Cholesterol - metabolism Elasticity EMBO20 Golgi Apparatus - chemistry Lipid Bilayers - chemistry lipid sorting membrane curvature membrane fission membrane tubules phase separation Phosphatidylcholines - chemistry Phosphatidylcholines - metabolism Photochemistry Sphingomyelins - chemistry Sphingomyelins - metabolism Stress, Mechanical |
| title | Role of curvature and phase transition in lipid sorting and fission of membrane tubules |
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