Effects of N-acetylcysteine on sciatic nerve healing: A histopathological, functional, and biochemical study of the rat sciatic nerve
This study aims to evaluate the histopathological, biochemical, and functional effects of N-acetylcysteine (NAC), which has antioxidant, anti-inflammatory, and cytoprotective activity, on nerve regeneration in rats with sciatic nerve crush (axonotmesis) injury. This study used 16 male Wistar rats, w...
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| Veröffentlicht in: | Joint diseases and related surgery 2024-07, Vol.35 (3), p.618-627 |
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| creator | Sarıtaş, Tahir Burak Ertürk, Cemil Büyükdoğan, Halil Yıldırım, Burak Gündüz, Nermin Selek, Şahabettin |
| description | This study aims to evaluate the histopathological, biochemical, and functional effects of N-acetylcysteine (NAC), which has antioxidant, anti-inflammatory, and cytoprotective activity, on nerve regeneration in rats with sciatic nerve crush (axonotmesis) injury.
This study used 16 male Wistar rats, which were divided into treatment and control groups. A standard axonotmesis-type surgical injury was induced in the left sciatic nerves of all rats. The treatment group was given 300 mg/kg of intraperitoneal NAC once a day, whereas the control group received an equal volume of saline solution. After conducting gait analyses, the sciatic functional index (SFI) was used for functional assessment. After gait analysis, all animals were euthanized. Blood samples were examined biochemically. The left sciatic nerves and left triceps surae muscles were examined histopathologically.
Histopathologically, the thickness of the perineurium, axonal degeneration, axonolysis, edema, inflammation, muscle atrophy, and muscle degeneration were all significantly lower in the treatment group (p |
| doi_str_mv | 10.52312/jdrs.2024.1784 |
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This study used 16 male Wistar rats, which were divided into treatment and control groups. A standard axonotmesis-type surgical injury was induced in the left sciatic nerves of all rats. The treatment group was given 300 mg/kg of intraperitoneal NAC once a day, whereas the control group received an equal volume of saline solution. After conducting gait analyses, the sciatic functional index (SFI) was used for functional assessment. After gait analysis, all animals were euthanized. Blood samples were examined biochemically. The left sciatic nerves and left triceps surae muscles were examined histopathologically.
Histopathologically, the thickness of the perineurium, axonal degeneration, axonolysis, edema, inflammation, muscle atrophy, and muscle degeneration were all significantly lower in the treatment group (p<0.05). Functionally, SFI-1, SFI-2, and SFI-3 were significantly higher in the treatment group (p<0.05). Biochemically, while the native thiol level and native thiol/total thiol ratio were significantly higher in the treatment group (p<0.003), the disulfide/total thiol ratio was significantly higher in the control group (p<0.005). Significant correlations were found between six of the seven gait parameters and the histopathological findings (p<0.05).
Our study results suggest that NAC may contribute positively to the histopathological and functional recovery of sciatic nerve injury in rats. Furthermore, NAC may have an antioxidant effect on thiol-disulfide homeostasis at a biochemical level. We believe that NAC has a stimulatory effect on healing following nerve injuries.</description><identifier>ISSN: 2687-4784</identifier><identifier>ISSN: 2687-4792</identifier><identifier>EISSN: 2687-4792</identifier><identifier>DOI: 10.52312/jdrs.2024.1784</identifier><identifier>PMID: 39189572</identifier><language>eng</language><publisher>Turkey: Bayçınar Medical Publishing</publisher><subject>Acetylcysteine - pharmacology ; Acetylcysteine - therapeutic use ; Animals ; Antioxidants - pharmacology ; Disease Models, Animal ; Male ; Nerve Regeneration - drug effects ; Original ; Peripheral Nerve Injuries - drug therapy ; Peripheral Nerve Injuries - pathology ; Rats ; Rats, Wistar ; Recovery of Function - drug effects ; Sciatic Nerve - drug effects ; Sciatic Nerve - injuries ; Sciatic Nerve - pathology ; Wound Healing - drug effects</subject><ispartof>Joint diseases and related surgery, 2024-07, Vol.35 (3), p.618-627</ispartof><rights>Copyright © 2024, Turkish Joint Diseases Foundation 2024 Turkish Joint Diseases Foundation</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><orcidid>0000-0003-1237-5486 ; 0000-0002-0202-444X ; 0000-0002-9225-917X ; 0000-0002-0720-874X ; 0000-0003-1235-3957 ; 0009-0001-3049-1723</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC11411894/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC11411894/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,724,777,781,882,27905,27906,53772,53774</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/39189572$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sarıtaş, Tahir Burak</creatorcontrib><creatorcontrib>Ertürk, Cemil</creatorcontrib><creatorcontrib>Büyükdoğan, Halil</creatorcontrib><creatorcontrib>Yıldırım, Burak</creatorcontrib><creatorcontrib>Gündüz, Nermin</creatorcontrib><creatorcontrib>Selek, Şahabettin</creatorcontrib><title>Effects of N-acetylcysteine on sciatic nerve healing: A histopathological, functional, and biochemical study of the rat sciatic nerve</title><title>Joint diseases and related surgery</title><addtitle>Jt Dis Relat Surg</addtitle><description>This study aims to evaluate the histopathological, biochemical, and functional effects of N-acetylcysteine (NAC), which has antioxidant, anti-inflammatory, and cytoprotective activity, on nerve regeneration in rats with sciatic nerve crush (axonotmesis) injury.
This study used 16 male Wistar rats, which were divided into treatment and control groups. A standard axonotmesis-type surgical injury was induced in the left sciatic nerves of all rats. The treatment group was given 300 mg/kg of intraperitoneal NAC once a day, whereas the control group received an equal volume of saline solution. After conducting gait analyses, the sciatic functional index (SFI) was used for functional assessment. After gait analysis, all animals were euthanized. Blood samples were examined biochemically. The left sciatic nerves and left triceps surae muscles were examined histopathologically.
Histopathologically, the thickness of the perineurium, axonal degeneration, axonolysis, edema, inflammation, muscle atrophy, and muscle degeneration were all significantly lower in the treatment group (p<0.05). Functionally, SFI-1, SFI-2, and SFI-3 were significantly higher in the treatment group (p<0.05). Biochemically, while the native thiol level and native thiol/total thiol ratio were significantly higher in the treatment group (p<0.003), the disulfide/total thiol ratio was significantly higher in the control group (p<0.005). Significant correlations were found between six of the seven gait parameters and the histopathological findings (p<0.05).
Our study results suggest that NAC may contribute positively to the histopathological and functional recovery of sciatic nerve injury in rats. Furthermore, NAC may have an antioxidant effect on thiol-disulfide homeostasis at a biochemical level. We believe that NAC has a stimulatory effect on healing following nerve injuries.</description><subject>Acetylcysteine - pharmacology</subject><subject>Acetylcysteine - therapeutic use</subject><subject>Animals</subject><subject>Antioxidants - pharmacology</subject><subject>Disease Models, Animal</subject><subject>Male</subject><subject>Nerve Regeneration - drug effects</subject><subject>Original</subject><subject>Peripheral Nerve Injuries - drug therapy</subject><subject>Peripheral Nerve Injuries - pathology</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Recovery of Function - drug effects</subject><subject>Sciatic Nerve - drug effects</subject><subject>Sciatic Nerve - injuries</subject><subject>Sciatic Nerve - pathology</subject><subject>Wound Healing - drug effects</subject><issn>2687-4784</issn><issn>2687-4792</issn><issn>2687-4792</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkcFO3DAQhi3UChDl3FvlYw_NYjvexO6lQghKJdRe2rPljMcbo6y9jR2kfQDem6TQFWgOHmn--Wc8HyEfOVutRc3Fxb0b80owIVe8VfKInIpGtZVstXh3yJU8Iec53zPGhBZ1y9gxOak1V3rdilPyeO09Qsk0efqzsoBlP8A-FwwRaYo0Q7AlAI04PiDt0Q4hbr7SS9qHXNLOlj4NaRPADl-onyKUkOKS2-hoFxL0uF2KNJfJ7ZchpUc62vLW-AN57-2Q8fzlPSN_bq5_X91Wd7--_7i6vKtg3rxUoFjtBDR6bT3n4EAqbBtlnes0cjdfoq0FeGg6kJ1uOm9RAHZOzf-t_Rxn5Nuz727qtugAYxntYHZj2Npxb5IN5m0lht5s0oPhXPL5ZHJ2-PziMKa_E-ZitiEDDoONmKZsaqZbqaVS9Sy9eJbCmHIe0R_mcGb-ATQLQLMANAvAuePT6_UO-v-46icRcpuS</recordid><startdate>20240708</startdate><enddate>20240708</enddate><creator>Sarıtaş, Tahir Burak</creator><creator>Ertürk, Cemil</creator><creator>Büyükdoğan, Halil</creator><creator>Yıldırım, Burak</creator><creator>Gündüz, Nermin</creator><creator>Selek, Şahabettin</creator><general>Bayçınar Medical Publishing</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0003-1237-5486</orcidid><orcidid>https://orcid.org/0000-0002-0202-444X</orcidid><orcidid>https://orcid.org/0000-0002-9225-917X</orcidid><orcidid>https://orcid.org/0000-0002-0720-874X</orcidid><orcidid>https://orcid.org/0000-0003-1235-3957</orcidid><orcidid>https://orcid.org/0009-0001-3049-1723</orcidid></search><sort><creationdate>20240708</creationdate><title>Effects of N-acetylcysteine on sciatic nerve healing: A histopathological, functional, and biochemical study of the rat sciatic nerve</title><author>Sarıtaş, Tahir Burak ; Ertürk, Cemil ; Büyükdoğan, Halil ; Yıldırım, Burak ; Gündüz, Nermin ; Selek, Şahabettin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c237t-c803d2c695af11cdc48e768addb9e1d202732cfc6bc4b96bfae2cebd83913f3f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Acetylcysteine - pharmacology</topic><topic>Acetylcysteine - therapeutic use</topic><topic>Animals</topic><topic>Antioxidants - pharmacology</topic><topic>Disease Models, Animal</topic><topic>Male</topic><topic>Nerve Regeneration - drug effects</topic><topic>Original</topic><topic>Peripheral Nerve Injuries - drug therapy</topic><topic>Peripheral Nerve Injuries - pathology</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Recovery of Function - drug effects</topic><topic>Sciatic Nerve - drug effects</topic><topic>Sciatic Nerve - injuries</topic><topic>Sciatic Nerve - pathology</topic><topic>Wound Healing - drug effects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sarıtaş, Tahir Burak</creatorcontrib><creatorcontrib>Ertürk, Cemil</creatorcontrib><creatorcontrib>Büyükdoğan, Halil</creatorcontrib><creatorcontrib>Yıldırım, Burak</creatorcontrib><creatorcontrib>Gündüz, Nermin</creatorcontrib><creatorcontrib>Selek, Şahabettin</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Joint diseases and related surgery</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sarıtaş, Tahir Burak</au><au>Ertürk, Cemil</au><au>Büyükdoğan, Halil</au><au>Yıldırım, Burak</au><au>Gündüz, Nermin</au><au>Selek, Şahabettin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of N-acetylcysteine on sciatic nerve healing: A histopathological, functional, and biochemical study of the rat sciatic nerve</atitle><jtitle>Joint diseases and related surgery</jtitle><addtitle>Jt Dis Relat Surg</addtitle><date>2024-07-08</date><risdate>2024</risdate><volume>35</volume><issue>3</issue><spage>618</spage><epage>627</epage><pages>618-627</pages><issn>2687-4784</issn><issn>2687-4792</issn><eissn>2687-4792</eissn><abstract>This study aims to evaluate the histopathological, biochemical, and functional effects of N-acetylcysteine (NAC), which has antioxidant, anti-inflammatory, and cytoprotective activity, on nerve regeneration in rats with sciatic nerve crush (axonotmesis) injury.
This study used 16 male Wistar rats, which were divided into treatment and control groups. A standard axonotmesis-type surgical injury was induced in the left sciatic nerves of all rats. The treatment group was given 300 mg/kg of intraperitoneal NAC once a day, whereas the control group received an equal volume of saline solution. After conducting gait analyses, the sciatic functional index (SFI) was used for functional assessment. After gait analysis, all animals were euthanized. Blood samples were examined biochemically. The left sciatic nerves and left triceps surae muscles were examined histopathologically.
Histopathologically, the thickness of the perineurium, axonal degeneration, axonolysis, edema, inflammation, muscle atrophy, and muscle degeneration were all significantly lower in the treatment group (p<0.05). Functionally, SFI-1, SFI-2, and SFI-3 were significantly higher in the treatment group (p<0.05). Biochemically, while the native thiol level and native thiol/total thiol ratio were significantly higher in the treatment group (p<0.003), the disulfide/total thiol ratio was significantly higher in the control group (p<0.005). Significant correlations were found between six of the seven gait parameters and the histopathological findings (p<0.05).
Our study results suggest that NAC may contribute positively to the histopathological and functional recovery of sciatic nerve injury in rats. Furthermore, NAC may have an antioxidant effect on thiol-disulfide homeostasis at a biochemical level. We believe that NAC has a stimulatory effect on healing following nerve injuries.</abstract><cop>Turkey</cop><pub>Bayçınar Medical Publishing</pub><pmid>39189572</pmid><doi>10.52312/jdrs.2024.1784</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0003-1237-5486</orcidid><orcidid>https://orcid.org/0000-0002-0202-444X</orcidid><orcidid>https://orcid.org/0000-0002-9225-917X</orcidid><orcidid>https://orcid.org/0000-0002-0720-874X</orcidid><orcidid>https://orcid.org/0000-0003-1235-3957</orcidid><orcidid>https://orcid.org/0009-0001-3049-1723</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Acetylcysteine - pharmacology Acetylcysteine - therapeutic use Animals Antioxidants - pharmacology Disease Models, Animal Male Nerve Regeneration - drug effects Original Peripheral Nerve Injuries - drug therapy Peripheral Nerve Injuries - pathology Rats Rats, Wistar Recovery of Function - drug effects Sciatic Nerve - drug effects Sciatic Nerve - injuries Sciatic Nerve - pathology Wound Healing - drug effects |
| title | Effects of N-acetylcysteine on sciatic nerve healing: A histopathological, functional, and biochemical study of the rat sciatic nerve |
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