Public Health Laboratory Service enzyme linked immunosorbent assay for detecting Toxoplasma specific IgM antibody
An enzyme linked immunosorbent assay (ELISA) based on the antibody class capture method for the detection of specific IgM against Toxoplasma gondii, using the microtitre plate format, was developed. Antigen binding was detected using a monoclonal antibody, CIE3, conjugated to horseradish peroxidase....
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| Veröffentlicht in: | Journal of clinical pathology 1987-03, Vol.40 (3), p.276-281 |
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| container_issue | 3 |
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| container_title | Journal of clinical pathology |
| container_volume | 40 |
| creator | Payne, R A Joynson, D H Balfour, A H Harford, J P Fleck, D G Mythen, M Saunders, R J |
| description | An enzyme linked immunosorbent assay (ELISA) based on the antibody class capture method for the detection of specific IgM against Toxoplasma gondii, using the microtitre plate format, was developed. Antigen binding was detected using a monoclonal antibody, CIE3, conjugated to horseradish peroxidase. Prior mixing of the conjugate and antigen improved the stability of these reagents as well as removing an incubation stage from the assay. The incubation time of less than four hours permits a rapid throughput of specimens. Using the assay, a total of 163 sera were examined in a three centre study and good agreement was found. Results were expressed as arbitrary enzyme immunoassay units (EIUs) against a freeze dried standard. Throughout the study the standard serum showed a coefficient of variation less than 10% across the microtitre plate. By measuring IgM titres in patients having toxoplasmic lymphadenopathy with a known date of onset, IgM class antibodies were shown to peak at two months, persisting for about six months. In addition, a case of laboratory acquired toxoplasmosis was monitored. Sera shown to contain rheumatoid factor and antinuclear factor did not give false positive results. This rapid, robust, and simplified assay is used by the Public Health Laboratory Service Toxoplasma Reference Units and will provide a standard with which other assays can be compared. |
| doi_str_mv | 10.1136/jcp.40.3.276 |
| format | Article |
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Antigen binding was detected using a monoclonal antibody, CIE3, conjugated to horseradish peroxidase. Prior mixing of the conjugate and antigen improved the stability of these reagents as well as removing an incubation stage from the assay. The incubation time of less than four hours permits a rapid throughput of specimens. Using the assay, a total of 163 sera were examined in a three centre study and good agreement was found. Results were expressed as arbitrary enzyme immunoassay units (EIUs) against a freeze dried standard. Throughout the study the standard serum showed a coefficient of variation less than 10% across the microtitre plate. By measuring IgM titres in patients having toxoplasmic lymphadenopathy with a known date of onset, IgM class antibodies were shown to peak at two months, persisting for about six months. In addition, a case of laboratory acquired toxoplasmosis was monitored. Sera shown to contain rheumatoid factor and antinuclear factor did not give false positive results. This rapid, robust, and simplified assay is used by the Public Health Laboratory Service Toxoplasma Reference Units and will provide a standard with which other assays can be compared.</description><identifier>ISSN: 0021-9746</identifier><identifier>EISSN: 1472-4146</identifier><identifier>DOI: 10.1136/jcp.40.3.276</identifier><identifier>PMID: 3558860</identifier><identifier>CODEN: JCPAAK</identifier><language>eng</language><publisher>London: BMJ Publishing Group Ltd and Association of Clinical Pathologists</publisher><subject>Animals ; Biological and medical sciences ; Clinical Laboratory Techniques ; England ; Enzyme-Linked Immunosorbent Assay ; Human protozoal diseases ; Immunoglobulin M - analysis ; Infectious diseases ; Medical sciences ; Methods ; Mice ; Mice, Inbred BALB C ; Parasitic diseases ; Protozoal diseases ; Quality Control ; Reference Standards ; Toxoplasma - immunology ; Toxoplasmosis ; Wales</subject><ispartof>Journal of clinical pathology, 1987-03, Vol.40 (3), p.276-281</ispartof><rights>1987 INIST-CNRS</rights><rights>Copyright BMJ Publishing Group LTD Mar 1987</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-b506t-66fc2df8887036fa19eca412028ab67b5856529cd5be2958b45235fe961e1223</citedby><cites>FETCH-LOGICAL-b506t-66fc2df8887036fa19eca412028ab67b5856529cd5be2958b45235fe961e1223</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1140898/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1140898/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=8171352$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3558860$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Payne, R A</creatorcontrib><creatorcontrib>Joynson, D H</creatorcontrib><creatorcontrib>Balfour, A H</creatorcontrib><creatorcontrib>Harford, J P</creatorcontrib><creatorcontrib>Fleck, D G</creatorcontrib><creatorcontrib>Mythen, M</creatorcontrib><creatorcontrib>Saunders, R J</creatorcontrib><title>Public Health Laboratory Service enzyme linked immunosorbent assay for detecting Toxoplasma specific IgM antibody</title><title>Journal of clinical pathology</title><addtitle>J Clin Pathol</addtitle><description>An enzyme linked immunosorbent assay (ELISA) based on the antibody class capture method for the detection of specific IgM against Toxoplasma gondii, using the microtitre plate format, was developed. Antigen binding was detected using a monoclonal antibody, CIE3, conjugated to horseradish peroxidase. Prior mixing of the conjugate and antigen improved the stability of these reagents as well as removing an incubation stage from the assay. The incubation time of less than four hours permits a rapid throughput of specimens. Using the assay, a total of 163 sera were examined in a three centre study and good agreement was found. Results were expressed as arbitrary enzyme immunoassay units (EIUs) against a freeze dried standard. Throughout the study the standard serum showed a coefficient of variation less than 10% across the microtitre plate. By measuring IgM titres in patients having toxoplasmic lymphadenopathy with a known date of onset, IgM class antibodies were shown to peak at two months, persisting for about six months. In addition, a case of laboratory acquired toxoplasmosis was monitored. Sera shown to contain rheumatoid factor and antinuclear factor did not give false positive results. This rapid, robust, and simplified assay is used by the Public Health Laboratory Service Toxoplasma Reference Units and will provide a standard with which other assays can be compared.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Clinical Laboratory Techniques</subject><subject>England</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Human protozoal diseases</subject><subject>Immunoglobulin M - analysis</subject><subject>Infectious diseases</subject><subject>Medical sciences</subject><subject>Methods</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Parasitic diseases</subject><subject>Protozoal diseases</subject><subject>Quality Control</subject><subject>Reference Standards</subject><subject>Toxoplasma - immunology</subject><subject>Toxoplasmosis</subject><subject>Wales</subject><issn>0021-9746</issn><issn>1472-4146</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1987</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp9kc9v0zAYhiMEGmVw44pkCQQXUvzbzmUSqoAOdYBYxYGLZTtO5y6JOzuZFv56PLWqgAMnH95Hr15_T1E8R3COEOHvtnY3p3BO5ljwB8UMUYFLiih_WMwgxKisBOWPiycpbSFERCByUpwQxqTkcFbcfBtN6y1YOt0OV2ClTYh6CHECly7eeuuA639NnQOt769dDXzXjX1IIRrXD0CnpCfQhAhqNzg7-H4D1uEu7FqdOg3Szlnf5PbzzQXQ_eBNqKenxaNGt8k9O7ynxfrjh_ViWa6-fjpfvF-VhkE-lJw3FteNlFJAwhuNKmc1RRhiqQ0XhknGGa5szYzDFZOGMkxY4yqOHMKYnBZn-9rdaDpX2zw36lbtou90nFTQXv2d9P5KbcKtQohCWclc8PpQEMPN6NKgOp-sa1vduzAmJQRliCOSwZf_gNswxj7_TSEhICUiXzpTb_eUjSGl6JrjFATVvUeVPSoKFVHZY8Zf_Dn_CB_E5fzVIdfJ6raJurc-HTGJsmd2f4Vyj_k0uLtjrOO14oIIpr78WCjBvy_Jz8vP6iLzb_a86bb_H_gbaJTDQQ</recordid><startdate>19870301</startdate><enddate>19870301</enddate><creator>Payne, R A</creator><creator>Joynson, D H</creator><creator>Balfour, A H</creator><creator>Harford, J P</creator><creator>Fleck, D G</creator><creator>Mythen, M</creator><creator>Saunders, R J</creator><general>BMJ Publishing Group Ltd and Association of Clinical Pathologists</general><general>BMJ</general><general>BMJ Publishing Group LTD</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>88I</scope><scope>8AF</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BTHHO</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19870301</creationdate><title>Public Health Laboratory Service enzyme linked immunosorbent assay for detecting Toxoplasma specific IgM antibody</title><author>Payne, R A ; Joynson, D H ; Balfour, A H ; Harford, J P ; Fleck, D G ; Mythen, M ; Saunders, R J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-b506t-66fc2df8887036fa19eca412028ab67b5856529cd5be2958b45235fe961e1223</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1987</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Clinical Laboratory Techniques</topic><topic>England</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Human protozoal diseases</topic><topic>Immunoglobulin M - analysis</topic><topic>Infectious diseases</topic><topic>Medical sciences</topic><topic>Methods</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Parasitic diseases</topic><topic>Protozoal diseases</topic><topic>Quality Control</topic><topic>Reference Standards</topic><topic>Toxoplasma - immunology</topic><topic>Toxoplasmosis</topic><topic>Wales</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Payne, R A</creatorcontrib><creatorcontrib>Joynson, D H</creatorcontrib><creatorcontrib>Balfour, A H</creatorcontrib><creatorcontrib>Harford, J P</creatorcontrib><creatorcontrib>Fleck, D G</creatorcontrib><creatorcontrib>Mythen, M</creatorcontrib><creatorcontrib>Saunders, R J</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>STEM Database</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>BMJ Journals</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of clinical pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Payne, R A</au><au>Joynson, D H</au><au>Balfour, A H</au><au>Harford, J P</au><au>Fleck, D G</au><au>Mythen, M</au><au>Saunders, R J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Public Health Laboratory Service enzyme linked immunosorbent assay for detecting Toxoplasma specific IgM antibody</atitle><jtitle>Journal of clinical pathology</jtitle><addtitle>J Clin Pathol</addtitle><date>1987-03-01</date><risdate>1987</risdate><volume>40</volume><issue>3</issue><spage>276</spage><epage>281</epage><pages>276-281</pages><issn>0021-9746</issn><eissn>1472-4146</eissn><coden>JCPAAK</coden><abstract>An enzyme linked immunosorbent assay (ELISA) based on the antibody class capture method for the detection of specific IgM against Toxoplasma gondii, using the microtitre plate format, was developed. Antigen binding was detected using a monoclonal antibody, CIE3, conjugated to horseradish peroxidase. Prior mixing of the conjugate and antigen improved the stability of these reagents as well as removing an incubation stage from the assay. The incubation time of less than four hours permits a rapid throughput of specimens. Using the assay, a total of 163 sera were examined in a three centre study and good agreement was found. Results were expressed as arbitrary enzyme immunoassay units (EIUs) against a freeze dried standard. Throughout the study the standard serum showed a coefficient of variation less than 10% across the microtitre plate. By measuring IgM titres in patients having toxoplasmic lymphadenopathy with a known date of onset, IgM class antibodies were shown to peak at two months, persisting for about six months. In addition, a case of laboratory acquired toxoplasmosis was monitored. Sera shown to contain rheumatoid factor and antinuclear factor did not give false positive results. This rapid, robust, and simplified assay is used by the Public Health Laboratory Service Toxoplasma Reference Units and will provide a standard with which other assays can be compared.</abstract><cop>London</cop><pub>BMJ Publishing Group Ltd and Association of Clinical Pathologists</pub><pmid>3558860</pmid><doi>10.1136/jcp.40.3.276</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of clinical pathology, 1987-03, Vol.40 (3), p.276-281 |
| issn | 0021-9746 1472-4146 |
| language | eng |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; PubMed Central; Alma/SFX Local Collection |
| subjects | Animals Biological and medical sciences Clinical Laboratory Techniques England Enzyme-Linked Immunosorbent Assay Human protozoal diseases Immunoglobulin M - analysis Infectious diseases Medical sciences Methods Mice Mice, Inbred BALB C Parasitic diseases Protozoal diseases Quality Control Reference Standards Toxoplasma - immunology Toxoplasmosis Wales |
| title | Public Health Laboratory Service enzyme linked immunosorbent assay for detecting Toxoplasma specific IgM antibody |
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