Modification of the PthA4 effector binding elements in Type I CsLOB1 promoter using Cas9/sgRNA to produce transgenic Duncan grapefruit alleviating XccΔpthA4:dCsLOB1.3 infection

Citrus canker caused by Xanthomonas citri subspecies citri (Xcc) is a severe disease for most commercial citrus cultivars and responsible for significant economic losses worldwide. Generating canker‐resistant citrus varieties will provide an efficient and sustainable solution to control citrus canke...

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Veröffentlicht in:	Plant biotechnology journal 2016-05, Vol.14 (5), p.1291-1301
Hauptverfasser:	Jia, Hongge, Orbovic, Vladimir, Jones, Jeffrey B, Wang, Nian
Format:	Artikel
Sprache:	eng
Schlagworte:	Alleles Binding Biotechnology Canker Cas9/sgRNA Citrus Citrus fruits Citrus paradisi - genetics Citrus paradisi - immunology Citrus sinensis Cloning CRISPR CsLOB1 Cultivars Deoxyribonucleic acid Disease Disease Resistance - genetics Disease susceptibility DNA Drinking water Economic impact financial economics Food Fruits Gene Editing Gene expression Genetic aspects Genetic transformation Genetic Vectors - genetics Genetically engineered foods genetically modified organisms genome editing Genomes Grapefruit grapefruits Health aspects Infections Mutation Mutation rates Neomycin Pathogenicity Pathogens Plant Diseases - immunology Plant Diseases - microbiology Plant Leaves - genetics Plant Leaves - immunology Plant Proteins - genetics Plant Proteins - metabolism Plants, Genetically Modified Promoter Regions, Genetic - genetics PthA Ribonucleic acid RNA Signs and symptoms Transgenic plants Virulence Factors Xanthomonas Xanthomonas - pathogenicity Xanthomonas citri
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