Purification and characterization of multiple forms of the pineapple-stem-derived cysteine proteinases ananain and comosain

A mixture of ananain (EC 3.4.22.31) and comosain purified from crude pineapple stem extract was found to contain numerous closely related enzyme forms. Chromatographic separation of the major enzyme forms was achieved after treatment of the mixture with thiol-modifying reagents: reversible modificat...

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Veröffentlicht in:	Biochemical journal 1994-08, Vol.301 (3), p.727-735
Hauptverfasser:	Napper, A.D, Bennett, S.P, Borowski, M, Holdridge, M.B, Leonard, M.J.C, Rogers, E.E, Duan, Y, Laursen, R.A, Reinhold, B, Shames, S.L
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Analytical, structural and metabolic biochemistry Ananas comosus Binding Sites Biological and medical sciences Bromelains - chemistry Cyanogen Bromide Cysteine Endopeptidases - chemistry Cysteine Endopeptidases - isolation & purification Cysteine Endopeptidases - metabolism cysteine proteinases Electrophoresis, Polyacrylamide Gel enzyme activity enzyme polymorphism Enzymes and enzyme inhibitors Fruit - enzymology Fundamental and applied biological sciences. Psychology Hydrolases Kinetics molecular conformation Molecular Sequence Data Peptide Fragments - chemistry Peptide Fragments - metabolism proteolysis purification Sequence Analysis stems structure Substrate Specificity
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