Investigation of alpha-deuterium kinetic isotope effects on the purine nucleoside phosphorylase reaction by the equilibrium-perturbation technique

1. alpha-Deuterium kinetic isotope effects on the phosphorolysis of inosine catalysed by Escherichia coli purine nucleoside phosphorylase were measured by the equilibrium-perturbation technique, by using the change in absorbance at 250 nm (approx. 20%). 2. Values of 2H(V/K) of 1.13(9) at pH 5.0, 1.1...

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Veröffentlicht in:	Biochemical journal 1989-01, Vol.257 (2), p.355-359
Hauptverfasser:	Lehikoinen, P K, Sinnott, M L, Krenitsky, T A
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Chemical Phenomena Chemistry Deuterium - pharmacology Escherichia coli Hydrogen-Ion Concentration Inosine - metabolism Isomerism Isotopes Kinetics Pentosyltransferases - metabolism Purine-Nucleoside Phosphorylase - metabolism Thermodynamics
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