In Vivo Luciferin-Luciferase Reaction in Micro-Mini Pigs Using Xenogeneic Rat Bone Marrow Transplantation
Luminescent technology based on the luciferin-luciferase reaction has been extensively employed across various disciplines as a quantitative imaging modality. Owing to its non-invasive imaging capacity, it has evolved as a valuable in vivo bioimaging tool, particularly in small animal models in fiel...
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| Veröffentlicht in: | International journal of molecular sciences 2024-08, Vol.25 (16), p.8609 |
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| creator | Abe, Tomoyuki Endo, Kazuhiro Hanazono, Yutaka Kobayashi, Eiji |
| description | Luminescent technology based on the luciferin-luciferase reaction has been extensively employed across various disciplines as a quantitative imaging modality. Owing to its non-invasive imaging capacity, it has evolved as a valuable in vivo bioimaging tool, particularly in small animal models in fields such as gene and cell therapies. We have previously successfully generated rats with a systemic expression of the
gene at the
locus. In this study, we transplanted bone marrow from these rats into micro-mini pigs and used in vivo imaging to non-invasively analyze the dynamics of the transplanted cells. In addition, we established that the rat-to-pig transplantation system is a discordant system, similar to the pig-to-human transplantation system. Thus, rat-to-pig transplantation may provide a clinically appropriate large animal model for pig-to-human xenotransplantation. |
| doi_str_mv | 10.3390/ijms25168609 |
| format | Article |
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gene at the
locus. In this study, we transplanted bone marrow from these rats into micro-mini pigs and used in vivo imaging to non-invasively analyze the dynamics of the transplanted cells. In addition, we established that the rat-to-pig transplantation system is a discordant system, similar to the pig-to-human transplantation system. Thus, rat-to-pig transplantation may provide a clinically appropriate large animal model for pig-to-human xenotransplantation.</description><identifier>ISSN: 1422-0067</identifier><identifier>ISSN: 1661-6596</identifier><identifier>EISSN: 1422-0067</identifier><identifier>DOI: 10.3390/ijms25168609</identifier><identifier>PMID: 39201296</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Animals ; Antibodies ; Blood pressure ; Bone marrow ; Bone Marrow Transplantation - methods ; Brief Report ; Experiments ; Firefly Luciferin - chemistry ; Firefly Luciferin - metabolism ; General anesthesia ; Genes ; Heterografts ; Hogs ; Humans ; Knee ; Luciferases - genetics ; Luciferases - metabolism ; Luminescent Measurements - methods ; Lungs ; Pulmonary embolisms ; Rats ; Swine ; Swine, Miniature ; Tomography ; Transplantation, Heterologous - methods</subject><ispartof>International journal of molecular sciences, 2024-08, Vol.25 (16), p.8609</ispartof><rights>2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2024 by the authors. 2024</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c300t-d26acdc462e06da86d7019a3c41f86f5e923ffc815bec9cc701514d9c6cbc3a43</cites><orcidid>0000-0001-8617-3778 ; 0000-0003-4610-7770 ; 0000-0001-8424-4843</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC11354750/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC11354750/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27903,27904,53769,53771</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/39201296$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Abe, Tomoyuki</creatorcontrib><creatorcontrib>Endo, Kazuhiro</creatorcontrib><creatorcontrib>Hanazono, Yutaka</creatorcontrib><creatorcontrib>Kobayashi, Eiji</creatorcontrib><title>In Vivo Luciferin-Luciferase Reaction in Micro-Mini Pigs Using Xenogeneic Rat Bone Marrow Transplantation</title><title>International journal of molecular sciences</title><addtitle>Int J Mol Sci</addtitle><description>Luminescent technology based on the luciferin-luciferase reaction has been extensively employed across various disciplines as a quantitative imaging modality. Owing to its non-invasive imaging capacity, it has evolved as a valuable in vivo bioimaging tool, particularly in small animal models in fields such as gene and cell therapies. We have previously successfully generated rats with a systemic expression of the
gene at the
locus. In this study, we transplanted bone marrow from these rats into micro-mini pigs and used in vivo imaging to non-invasively analyze the dynamics of the transplanted cells. In addition, we established that the rat-to-pig transplantation system is a discordant system, similar to the pig-to-human transplantation system. Thus, rat-to-pig transplantation may provide a clinically appropriate large animal model for pig-to-human xenotransplantation.</description><subject>Animals</subject><subject>Antibodies</subject><subject>Blood pressure</subject><subject>Bone marrow</subject><subject>Bone Marrow Transplantation - methods</subject><subject>Brief Report</subject><subject>Experiments</subject><subject>Firefly Luciferin - chemistry</subject><subject>Firefly Luciferin - metabolism</subject><subject>General anesthesia</subject><subject>Genes</subject><subject>Heterografts</subject><subject>Hogs</subject><subject>Humans</subject><subject>Knee</subject><subject>Luciferases - genetics</subject><subject>Luciferases - metabolism</subject><subject>Luminescent Measurements - methods</subject><subject>Lungs</subject><subject>Pulmonary embolisms</subject><subject>Rats</subject><subject>Swine</subject><subject>Swine, Miniature</subject><subject>Tomography</subject><subject>Transplantation, Heterologous - methods</subject><issn>1422-0067</issn><issn>1661-6596</issn><issn>1422-0067</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNpdkc1LHEEQxRuJRGNyyzk05OIhY_pjpmf6JIloIuySIBq8Nb01NWstu91r94yS_z6zuMqaUz2oH4969Rj7KMWJ1lZ8pcUqq0qaxgi7xw5lqVQhhKnf7OgD9i7nhRBKq8q-ZQfaKiGVNYeMLgP_Qw-RTwagDhOFYqt8Rn6FHnqKgVPgU4IUiykF4r9pnvlNpjDntxjiHAMS8Cvf8-8xIJ_6lOIjv04-5PXSh95vPN6z_c4vM37YziN2c3F-ffazmPz6cXn2bVKAFqIvWmU8tFAahcK0vjFtLaT1GkrZNaar0CrdddDIaoZgAcZtJcvWgoEZaF_qI3b65LseZitsAUOf_NKtE618-uuiJ_d6E-jOzeODk1JXZV2J0eF465Di_YC5dyvKgMsxCsYhOy2slaXWeoN-_g9dxCGFMd-Gqm3Z1LoZqS9P1PjBnBN2L9dI4TYlut0SR_zTboIX-Lk1_Q84UZmH</recordid><startdate>20240807</startdate><enddate>20240807</enddate><creator>Abe, Tomoyuki</creator><creator>Endo, Kazuhiro</creator><creator>Hanazono, Yutaka</creator><creator>Kobayashi, Eiji</creator><general>MDPI AG</general><general>MDPI</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>MBDVC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0001-8617-3778</orcidid><orcidid>https://orcid.org/0000-0003-4610-7770</orcidid><orcidid>https://orcid.org/0000-0001-8424-4843</orcidid></search><sort><creationdate>20240807</creationdate><title>In Vivo Luciferin-Luciferase Reaction in Micro-Mini Pigs Using Xenogeneic Rat Bone Marrow Transplantation</title><author>Abe, Tomoyuki ; Endo, Kazuhiro ; Hanazono, Yutaka ; Kobayashi, Eiji</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c300t-d26acdc462e06da86d7019a3c41f86f5e923ffc815bec9cc701514d9c6cbc3a43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Animals</topic><topic>Antibodies</topic><topic>Blood pressure</topic><topic>Bone marrow</topic><topic>Bone Marrow Transplantation - methods</topic><topic>Brief Report</topic><topic>Experiments</topic><topic>Firefly Luciferin - chemistry</topic><topic>Firefly Luciferin - metabolism</topic><topic>General anesthesia</topic><topic>Genes</topic><topic>Heterografts</topic><topic>Hogs</topic><topic>Humans</topic><topic>Knee</topic><topic>Luciferases - genetics</topic><topic>Luciferases - metabolism</topic><topic>Luminescent Measurements - methods</topic><topic>Lungs</topic><topic>Pulmonary embolisms</topic><topic>Rats</topic><topic>Swine</topic><topic>Swine, Miniature</topic><topic>Tomography</topic><topic>Transplantation, Heterologous - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Abe, Tomoyuki</creatorcontrib><creatorcontrib>Endo, Kazuhiro</creatorcontrib><creatorcontrib>Hanazono, Yutaka</creatorcontrib><creatorcontrib>Kobayashi, Eiji</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Research Library (Corporate)</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>International journal of molecular sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Abe, Tomoyuki</au><au>Endo, Kazuhiro</au><au>Hanazono, Yutaka</au><au>Kobayashi, Eiji</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In Vivo Luciferin-Luciferase Reaction in Micro-Mini Pigs Using Xenogeneic Rat Bone Marrow Transplantation</atitle><jtitle>International journal of molecular sciences</jtitle><addtitle>Int J Mol Sci</addtitle><date>2024-08-07</date><risdate>2024</risdate><volume>25</volume><issue>16</issue><spage>8609</spage><pages>8609-</pages><issn>1422-0067</issn><issn>1661-6596</issn><eissn>1422-0067</eissn><abstract>Luminescent technology based on the luciferin-luciferase reaction has been extensively employed across various disciplines as a quantitative imaging modality. Owing to its non-invasive imaging capacity, it has evolved as a valuable in vivo bioimaging tool, particularly in small animal models in fields such as gene and cell therapies. We have previously successfully generated rats with a systemic expression of the
gene at the
locus. In this study, we transplanted bone marrow from these rats into micro-mini pigs and used in vivo imaging to non-invasively analyze the dynamics of the transplanted cells. In addition, we established that the rat-to-pig transplantation system is a discordant system, similar to the pig-to-human transplantation system. Thus, rat-to-pig transplantation may provide a clinically appropriate large animal model for pig-to-human xenotransplantation.</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>39201296</pmid><doi>10.3390/ijms25168609</doi><orcidid>https://orcid.org/0000-0001-8617-3778</orcidid><orcidid>https://orcid.org/0000-0003-4610-7770</orcidid><orcidid>https://orcid.org/0000-0001-8424-4843</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | International journal of molecular sciences, 2024-08, Vol.25 (16), p.8609 |
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| source | MDPI - Multidisciplinary Digital Publishing Institute; MEDLINE; PubMed Central; EZB Electronic Journals Library |
| subjects | Animals Antibodies Blood pressure Bone marrow Bone Marrow Transplantation - methods Brief Report Experiments Firefly Luciferin - chemistry Firefly Luciferin - metabolism General anesthesia Genes Heterografts Hogs Humans Knee Luciferases - genetics Luciferases - metabolism Luminescent Measurements - methods Lungs Pulmonary embolisms Rats Swine Swine, Miniature Tomography Transplantation, Heterologous - methods |
| title | In Vivo Luciferin-Luciferase Reaction in Micro-Mini Pigs Using Xenogeneic Rat Bone Marrow Transplantation |
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