Fibrosis-Related microRNAs in Crohn's Disease with Fibrostenosis and Inflammatory Stenosis
Crohn's disease (CD) is frequently complicated by strictures that can be either inflammatory or fibrostenotic. This distinction is important for deciding the best treatment course, but it can be difficult to determine clinically, sometimes even by advanced imaging techniques. We performed miRNA...
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| Veröffentlicht in: | International journal of molecular sciences 2024-08, Vol.25 (16), p.8826 |
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| creator | Jerala, Miha Remic, Tinkara Hauptman, Nina Zidar, Nina |
| description | Crohn's disease (CD) is frequently complicated by strictures that can be either inflammatory or fibrostenotic. This distinction is important for deciding the best treatment course, but it can be difficult to determine clinically, sometimes even by advanced imaging techniques. We performed miRNA PCR panel screening on pooled samples of ileum with CD fibrostenosis or inflammatory stenosis. Eight miRNAs with profibrotic (
,
and
), or fibroprotective (
,
,
,
and
) functions described in the literature were selected for validation on 20 samples each of CD with fibrostenosis or inflammatory stenosis, with a separate sampling of the submucosa and subserosa. The results showed significant differences between the groups in subserosal samples, with upregulation of profibrotic miRNAs and downregulation of fibroprotective miRNAs in fibrostenosis compared to inflammatory stenosis. Only
showed a significant difference in the submucosa. There were significant differences in miRNA expression between subserosa and submucosa. Our results provide further evidence that the major differences between fibrostenosis and inflammatory stenosis are located in the subserosa, which is inaccessible to endoscopic sampling, highlighting the need for cross-sectional imaging or serological markers. We identify several miRNAs previously not connected to fibrosis in CD, which could potentially serve as biomarkers of fibrostenosis. |
| doi_str_mv | 10.3390/ijms25168826 |
| format | Article |
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,
and
), or fibroprotective (
,
,
,
and
) functions described in the literature were selected for validation on 20 samples each of CD with fibrostenosis or inflammatory stenosis, with a separate sampling of the submucosa and subserosa. The results showed significant differences between the groups in subserosal samples, with upregulation of profibrotic miRNAs and downregulation of fibroprotective miRNAs in fibrostenosis compared to inflammatory stenosis. Only
showed a significant difference in the submucosa. There were significant differences in miRNA expression between subserosa and submucosa. Our results provide further evidence that the major differences between fibrostenosis and inflammatory stenosis are located in the subserosa, which is inaccessible to endoscopic sampling, highlighting the need for cross-sectional imaging or serological markers. We identify several miRNAs previously not connected to fibrosis in CD, which could potentially serve as biomarkers of fibrostenosis.</description><identifier>ISSN: 1422-0067</identifier><identifier>ISSN: 1661-6596</identifier><identifier>EISSN: 1422-0067</identifier><identifier>DOI: 10.3390/ijms25168826</identifier><identifier>PMID: 39201512</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Adult ; Biopsy ; Constriction, Pathologic - genetics ; Crohn Disease - genetics ; Crohn Disease - metabolism ; Crohn Disease - pathology ; Crohn's disease ; Endoscopy ; Female ; Fibrosis - genetics ; Gene Expression Profiling ; Gene Expression Regulation ; Humans ; Ileum - metabolism ; Ileum - pathology ; Male ; MicroRNAs ; MicroRNAs - genetics ; Middle Aged</subject><ispartof>International journal of molecular sciences, 2024-08, Vol.25 (16), p.8826</ispartof><rights>2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2024 by the authors. 2024</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c300t-1e1f2580d7a000cb980c22794f7c6227f6c8cead73c2a22270929293200aa7a53</cites><orcidid>0000-0001-6866-3220 ; 0000-0002-0153-0260 ; 0000-0002-2391-8776 ; 0000-0002-7575-7716</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC11354456/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC11354456/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,724,777,781,882,27905,27906,53772,53774</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/39201512$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jerala, Miha</creatorcontrib><creatorcontrib>Remic, Tinkara</creatorcontrib><creatorcontrib>Hauptman, Nina</creatorcontrib><creatorcontrib>Zidar, Nina</creatorcontrib><title>Fibrosis-Related microRNAs in Crohn's Disease with Fibrostenosis and Inflammatory Stenosis</title><title>International journal of molecular sciences</title><addtitle>Int J Mol Sci</addtitle><description>Crohn's disease (CD) is frequently complicated by strictures that can be either inflammatory or fibrostenotic. This distinction is important for deciding the best treatment course, but it can be difficult to determine clinically, sometimes even by advanced imaging techniques. We performed miRNA PCR panel screening on pooled samples of ileum with CD fibrostenosis or inflammatory stenosis. Eight miRNAs with profibrotic (
,
and
), or fibroprotective (
,
,
,
and
) functions described in the literature were selected for validation on 20 samples each of CD with fibrostenosis or inflammatory stenosis, with a separate sampling of the submucosa and subserosa. The results showed significant differences between the groups in subserosal samples, with upregulation of profibrotic miRNAs and downregulation of fibroprotective miRNAs in fibrostenosis compared to inflammatory stenosis. Only
showed a significant difference in the submucosa. There were significant differences in miRNA expression between subserosa and submucosa. Our results provide further evidence that the major differences between fibrostenosis and inflammatory stenosis are located in the subserosa, which is inaccessible to endoscopic sampling, highlighting the need for cross-sectional imaging or serological markers. We identify several miRNAs previously not connected to fibrosis in CD, which could potentially serve as biomarkers of fibrostenosis.</description><subject>Adult</subject><subject>Biopsy</subject><subject>Constriction, Pathologic - genetics</subject><subject>Crohn Disease - genetics</subject><subject>Crohn Disease - metabolism</subject><subject>Crohn Disease - pathology</subject><subject>Crohn's disease</subject><subject>Endoscopy</subject><subject>Female</subject><subject>Fibrosis - genetics</subject><subject>Gene Expression Profiling</subject><subject>Gene Expression Regulation</subject><subject>Humans</subject><subject>Ileum - metabolism</subject><subject>Ileum - pathology</subject><subject>Male</subject><subject>MicroRNAs</subject><subject>MicroRNAs - genetics</subject><subject>Middle Aged</subject><issn>1422-0067</issn><issn>1661-6596</issn><issn>1422-0067</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNpdkctLAzEQxoMotj5uniXgQQ-uTpJ9nqTUV6Eo-Lh4CWk2a1N2k5rsKv3vTWmVKnOYIfObj5l8CB0RuGCsgEs9azxNSJrnNN1CfRJTGgGk2fZG3UN73s8AKKNJsYt6rKBAEkL76O1WT5z12kdPqhatKnGjpbNPDwOPtcFDZ6fm1ONr7ZXwCn_pdopXI60yyzksTIlHpqpF04jWugV-XncO0E4laq8O13kfvd7evAzvo_Hj3Wg4GEeSAbQRUaSiSQ5lJgBAToocJKVZEVeZTENRpTKXSpQZk1TQ8AAFDcEogBCZSNg-ulrpzrtJo0qpTOtEzedON8ItuBWa_-0YPeXv9pMTwpI4TtKgcLZWcPajU77ljfZS1bUwynaeMygKEjOWZQE9-YfObOdMuG9Jha0pJHmgzldU-Ervnap-tyHAl67xTdcCfrx5wS_8YxP7BmcukvI</recordid><startdate>20240813</startdate><enddate>20240813</enddate><creator>Jerala, Miha</creator><creator>Remic, Tinkara</creator><creator>Hauptman, Nina</creator><creator>Zidar, Nina</creator><general>MDPI AG</general><general>MDPI</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>MBDVC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0001-6866-3220</orcidid><orcidid>https://orcid.org/0000-0002-0153-0260</orcidid><orcidid>https://orcid.org/0000-0002-2391-8776</orcidid><orcidid>https://orcid.org/0000-0002-7575-7716</orcidid></search><sort><creationdate>20240813</creationdate><title>Fibrosis-Related microRNAs in Crohn's Disease with Fibrostenosis and Inflammatory Stenosis</title><author>Jerala, Miha ; Remic, Tinkara ; Hauptman, Nina ; Zidar, Nina</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c300t-1e1f2580d7a000cb980c22794f7c6227f6c8cead73c2a22270929293200aa7a53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Adult</topic><topic>Biopsy</topic><topic>Constriction, Pathologic - genetics</topic><topic>Crohn Disease - genetics</topic><topic>Crohn Disease - metabolism</topic><topic>Crohn Disease - pathology</topic><topic>Crohn's disease</topic><topic>Endoscopy</topic><topic>Female</topic><topic>Fibrosis - genetics</topic><topic>Gene Expression Profiling</topic><topic>Gene Expression Regulation</topic><topic>Humans</topic><topic>Ileum - metabolism</topic><topic>Ileum - pathology</topic><topic>Male</topic><topic>MicroRNAs</topic><topic>MicroRNAs - genetics</topic><topic>Middle Aged</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jerala, Miha</creatorcontrib><creatorcontrib>Remic, Tinkara</creatorcontrib><creatorcontrib>Hauptman, Nina</creatorcontrib><creatorcontrib>Zidar, Nina</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Research Library (Corporate)</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>International journal of molecular sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jerala, Miha</au><au>Remic, Tinkara</au><au>Hauptman, Nina</au><au>Zidar, Nina</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Fibrosis-Related microRNAs in Crohn's Disease with Fibrostenosis and Inflammatory Stenosis</atitle><jtitle>International journal of molecular sciences</jtitle><addtitle>Int J Mol Sci</addtitle><date>2024-08-13</date><risdate>2024</risdate><volume>25</volume><issue>16</issue><spage>8826</spage><pages>8826-</pages><issn>1422-0067</issn><issn>1661-6596</issn><eissn>1422-0067</eissn><abstract>Crohn's disease (CD) is frequently complicated by strictures that can be either inflammatory or fibrostenotic. This distinction is important for deciding the best treatment course, but it can be difficult to determine clinically, sometimes even by advanced imaging techniques. We performed miRNA PCR panel screening on pooled samples of ileum with CD fibrostenosis or inflammatory stenosis. Eight miRNAs with profibrotic (
,
and
), or fibroprotective (
,
,
,
and
) functions described in the literature were selected for validation on 20 samples each of CD with fibrostenosis or inflammatory stenosis, with a separate sampling of the submucosa and subserosa. The results showed significant differences between the groups in subserosal samples, with upregulation of profibrotic miRNAs and downregulation of fibroprotective miRNAs in fibrostenosis compared to inflammatory stenosis. Only
showed a significant difference in the submucosa. There were significant differences in miRNA expression between subserosa and submucosa. Our results provide further evidence that the major differences between fibrostenosis and inflammatory stenosis are located in the subserosa, which is inaccessible to endoscopic sampling, highlighting the need for cross-sectional imaging or serological markers. We identify several miRNAs previously not connected to fibrosis in CD, which could potentially serve as biomarkers of fibrostenosis.</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>39201512</pmid><doi>10.3390/ijms25168826</doi><orcidid>https://orcid.org/0000-0001-6866-3220</orcidid><orcidid>https://orcid.org/0000-0002-0153-0260</orcidid><orcidid>https://orcid.org/0000-0002-2391-8776</orcidid><orcidid>https://orcid.org/0000-0002-7575-7716</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | International journal of molecular sciences, 2024-08, Vol.25 (16), p.8826 |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; MDPI - Multidisciplinary Digital Publishing Institute; PubMed Central |
| subjects | Adult Biopsy Constriction, Pathologic - genetics Crohn Disease - genetics Crohn Disease - metabolism Crohn Disease - pathology Crohn's disease Endoscopy Female Fibrosis - genetics Gene Expression Profiling Gene Expression Regulation Humans Ileum - metabolism Ileum - pathology Male MicroRNAs MicroRNAs - genetics Middle Aged |
| title | Fibrosis-Related microRNAs in Crohn's Disease with Fibrostenosis and Inflammatory Stenosis |
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