In vitro culture of mechanically isolated murine primary follicles in the presence of human platelet lysate PLTMax
To develop a system for the culture of murine preantral ovarian follicles using Human Serum Albumin (HSA) and Human Platelet Lysate (PLTMax). Mechanically isolated preantral follicles (N=146) were obtained from Swiss mice and cultured in DMEM:F12 medium for ten days in a 96-well plate with conical b...
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| Veröffentlicht in: | JBRA assisted reproduction 2024-01, Vol.28 (3), p.410-417 |
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| creator | Amaral, Vera Lucia Lângaro Nunes, Jhuly Laurentino Salvador, Rafael Alonso Senn, Alfred Paul Santos, Tiago Góss Dos |
| description | To develop a system for the culture of murine preantral ovarian follicles using Human Serum Albumin (HSA) and Human Platelet Lysate (PLTMax).
Mechanically isolated preantral follicles (N=146) were obtained from Swiss mice and cultured in DMEM:F12 medium for ten days in a 96-well plate with conical bottom. The medium was supplemented with penicillin, streptomycin, and equine chorionic gonadotropin. Additional proteins were tested in 4 test groups: G1: human serum albumin (HSA), G2: human platelet lysate (PLTM), and G3 and G4: HSA + PLTMax at lower and higher concentrations, respectively. Cellular vitality and oocyte morphology were evaluated on day 11 of culture.
The highest follicular growth (3.4 fold) was achieved in HSA (G1), while a significantly lower (1.8 fold) growth was achieved in the presence of PLTM (G2, G4) and even further reduced (1.2 fold) when HSA and PLTM were combined (G3). Cellular vitality was close to 70-80% among the four groups, and the highest number of intact oocytes were found in G1.
PLTM did not improve follicular development and oocyte maturation compared to HSA but preserved cell vitality. |
| doi_str_mv | 10.5935/1518-0557.20240008 |
| format | Article |
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Mechanically isolated preantral follicles (N=146) were obtained from Swiss mice and cultured in DMEM:F12 medium for ten days in a 96-well plate with conical bottom. The medium was supplemented with penicillin, streptomycin, and equine chorionic gonadotropin. Additional proteins were tested in 4 test groups: G1: human serum albumin (HSA), G2: human platelet lysate (PLTM), and G3 and G4: HSA + PLTMax at lower and higher concentrations, respectively. Cellular vitality and oocyte morphology were evaluated on day 11 of culture.
The highest follicular growth (3.4 fold) was achieved in HSA (G1), while a significantly lower (1.8 fold) growth was achieved in the presence of PLTM (G2, G4) and even further reduced (1.2 fold) when HSA and PLTM were combined (G3). Cellular vitality was close to 70-80% among the four groups, and the highest number of intact oocytes were found in G1.
PLTM did not improve follicular development and oocyte maturation compared to HSA but preserved cell vitality.</description><identifier>ISSN: 1518-0557</identifier><identifier>ISSN: 1517-5693</identifier><identifier>EISSN: 1518-0557</identifier><identifier>DOI: 10.5935/1518-0557.20240008</identifier><identifier>PMID: 38446749</identifier><language>eng</language><publisher>Brazil: Sociedade Brasileira de Reprodução Humana (Brazilian Society of Assisted Reproduction)</publisher><subject>Cancer therapies ; Cryopreservation ; Females ; Fertility ; Follicles ; Growth factors ; Insulin ; Original ; Ovaries ; Penicillin ; Proteins ; Public domain ; Selenium</subject><ispartof>JBRA assisted reproduction, 2024-01, Vol.28 (3), p.410-417</ispartof><rights>2024. This work is published under https://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC11349267/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC11349267/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27903,27904,53769,53771</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38446749$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Amaral, Vera Lucia Lângaro</creatorcontrib><creatorcontrib>Nunes, Jhuly Laurentino</creatorcontrib><creatorcontrib>Salvador, Rafael Alonso</creatorcontrib><creatorcontrib>Senn, Alfred Paul</creatorcontrib><creatorcontrib>Santos, Tiago Góss Dos</creatorcontrib><title>In vitro culture of mechanically isolated murine primary follicles in the presence of human platelet lysate PLTMax</title><title>JBRA assisted reproduction</title><addtitle>JBRA Assist Reprod</addtitle><description>To develop a system for the culture of murine preantral ovarian follicles using Human Serum Albumin (HSA) and Human Platelet Lysate (PLTMax).
Mechanically isolated preantral follicles (N=146) were obtained from Swiss mice and cultured in DMEM:F12 medium for ten days in a 96-well plate with conical bottom. The medium was supplemented with penicillin, streptomycin, and equine chorionic gonadotropin. Additional proteins were tested in 4 test groups: G1: human serum albumin (HSA), G2: human platelet lysate (PLTM), and G3 and G4: HSA + PLTMax at lower and higher concentrations, respectively. Cellular vitality and oocyte morphology were evaluated on day 11 of culture.
The highest follicular growth (3.4 fold) was achieved in HSA (G1), while a significantly lower (1.8 fold) growth was achieved in the presence of PLTM (G2, G4) and even further reduced (1.2 fold) when HSA and PLTM were combined (G3). Cellular vitality was close to 70-80% among the four groups, and the highest number of intact oocytes were found in G1.
PLTM did not improve follicular development and oocyte maturation compared to HSA but preserved cell vitality.</description><subject>Cancer therapies</subject><subject>Cryopreservation</subject><subject>Females</subject><subject>Fertility</subject><subject>Follicles</subject><subject>Growth factors</subject><subject>Insulin</subject><subject>Original</subject><subject>Ovaries</subject><subject>Penicillin</subject><subject>Proteins</subject><subject>Public domain</subject><subject>Selenium</subject><issn>1518-0557</issn><issn>1517-5693</issn><issn>1518-0557</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNpdkU9P3DAQxa2KqiDgC3CoLPXSy4L_JvYJVYgWpEX0AGfLcSZdI8de7ASx354ElhXlNKOZ33ua0UPohJJTqbk8o5KqBZGyPmWECUKI-oIOdsO9D_0-Oi7lYSKopowL8g3tcyVEVQt9gPJ1xE9-yAm7MQxjBpw63INb2eidDWGDfUnBDtDifsw-Al5n39u8wV0KwbsABfuIh9W8gALRvTqsxt5GvJ6FAQYcNmXq8N_l3Y19PkJfOxsKHG_rIbr_fXl3cbVY3v65vvi1XDhO2bDgoKtKO9kwpzopVKVA10wIRx1rmeKSVrxtndRN1XImG66pJo1sqAXK6pbwQ3T-5rsemx5aB3HINpjt_SZZb_7fRL8y_9KToZQLzap6cvi5dcjpcYQymN4XByHYCGkshmnBqKol1xP64xP6kMYcp_8MJ1qzWsmaTRR7o1xOpWTodtdQYuZYzZyamVMz77FOou8f_9hJ3kPkLyTJnhY</recordid><startdate>20240101</startdate><enddate>20240101</enddate><creator>Amaral, Vera Lucia Lângaro</creator><creator>Nunes, Jhuly Laurentino</creator><creator>Salvador, Rafael Alonso</creator><creator>Senn, Alfred Paul</creator><creator>Santos, Tiago Góss Dos</creator><general>Sociedade Brasileira de Reprodução Humana (Brazilian Society of Assisted Reproduction)</general><general>Brazilian Society of Assisted Reproduction</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20240101</creationdate><title>In vitro culture of mechanically isolated murine primary follicles in the presence of human platelet lysate PLTMax</title><author>Amaral, Vera Lucia Lângaro ; 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Mechanically isolated preantral follicles (N=146) were obtained from Swiss mice and cultured in DMEM:F12 medium for ten days in a 96-well plate with conical bottom. The medium was supplemented with penicillin, streptomycin, and equine chorionic gonadotropin. Additional proteins were tested in 4 test groups: G1: human serum albumin (HSA), G2: human platelet lysate (PLTM), and G3 and G4: HSA + PLTMax at lower and higher concentrations, respectively. Cellular vitality and oocyte morphology were evaluated on day 11 of culture.
The highest follicular growth (3.4 fold) was achieved in HSA (G1), while a significantly lower (1.8 fold) growth was achieved in the presence of PLTM (G2, G4) and even further reduced (1.2 fold) when HSA and PLTM were combined (G3). Cellular vitality was close to 70-80% among the four groups, and the highest number of intact oocytes were found in G1.
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| subjects | Cancer therapies Cryopreservation Females Fertility Follicles Growth factors Insulin Original Ovaries Penicillin Proteins Public domain Selenium |
| title | In vitro culture of mechanically isolated murine primary follicles in the presence of human platelet lysate PLTMax |
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