Characterization of the gene celD and its encoded product 1,4-beta-D-glucan glucohydrolase D from Pseudomonas fluorescens subsp. cellulosa

A genomic library of Pseudomonas fluorescens subsp. cellulosa DNA constructed in pUC18 and expressed in Escherichia coli was screened for recombinants expressing 4-methylumbelliferyl beta-D-glucoside hydrolysing activity (MUGase). A single MUGase-positive clone was isolated. The MUGase hydrolysed ce...

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Veröffentlicht in:	Biochemical journal 1992-08, Vol.285 (3), p.947-955
Hauptverfasser:	Rixon, J.E, Ferreira, L.M.A, Durrant, A.J, Laurie, J.I, Hazlewood, G.P, Gilbert, H.J
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence amino acid sequences Analytical, structural and metabolic biochemistry Base Sequence beta-Glucosidase - chemistry beta-Glucosidase - genetics Biological and medical sciences Blotting, Western celD gene cellulolytic microorganisms clones Cloning, Molecular ddbj/x65527 embl/x65527 enzyme activity Enzymes and enzyme inhibitors Escherichia coli - enzymology Escherichia coli - genetics Fundamental and applied biological sciences. Psychology genbank/x65527 Gene Expression genes genetic recombination Glucan 1,4-beta-Glucosidase Hydrolases Molecular Sequence Data nucleotide sequence nucleotide sequences O-glycoside hydrolases plasmids predictions Pseudomonas fluorescens Pseudomonas fluorescens - enzymology Pseudomonas fluorescens - genetics Pseudomonas fluorescens cellulosa Recombinant Proteins - metabolism Restriction Mapping sequence alignment Sequence Homology, Nucleic Acid structure-activity relationships subsp.cellulosa Substrate Specificity
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