Kinetic resolution of the separate GLUT1 and Glut4 glucose transport activities in 3T3-L1 cells

A bis-mannose-photolabel-displacement method has been developed for resolving the separate kinetic properties of the glucose transporters GLUT1 and GLUT4, which are both present in 3T3-L1 cells. We have quantified the cell-surface transporter abundance (Bmax.) for the two isoforms by displacing radi...

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Veröffentlicht in:	Biochemical journal 1992-05, Vol.284 (1), p.275-282
Hauptverfasser:	PALFREYMAN, R. W, CLARK, A. E, DENTON, R. M, HOLMAN, G. D, KOZKA, I. J
Format:	Artikel
Sprache:	eng
Schlagworte:	3-O-Methylglucose 3T3 Cells - metabolism Animals Azides - pharmacokinetics Biological and medical sciences Biological Transport Cell Membrane - metabolism Cell Membrane Permeability Cell physiology comparison Disaccharides - pharmacokinetics fibroblasts Fundamental and applied biological sciences. Psychology GLUT1 protein GLUT4 protein Glycosides Insulin - pharmacology Isomerism Kinetics Membrane and intracellular transports Methylglucosides - pharmacokinetics Mice Molecular and cellular biology Monosaccharide Transport Proteins - metabolism Monosaccharide Transport Proteins - pharmacokinetics Precipitin Tests Propylamines transport
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