The pKa of the catalytic histidine residue to chloramphenicol acetyltransferase

A catalytically essential histidine residue (His-195) of chloramphenicol acetyltransferase (CAT) acts as a general base in catalysis, abstracting a proton from the primary hydroxy group of chloramphenicol. The pKa of His-195 has been determined from the pH-dependence of chemical modification. Both m...

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Veröffentlicht in:	Biochemical journal 1993-02, Vol.290 (Pt 1), p.15-19
Hauptverfasser:	LEWENDON, A, SHAW, W. V
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical, structural and metabolic biochemistry Benzenesulfonates - pharmacology Binding Sites Biological and medical sciences Catalysis Chloramphenicol O-Acetyltransferase - antagonists & inhibitors Chloramphenicol O-Acetyltransferase - chemistry Chloramphenicol O-Acetyltransferase - metabolism Cysteine - chemistry Enzymes and enzyme inhibitors Escherichia coli - enzymology Fundamental and applied biological sciences. Psychology Histidine - chemistry Hydrogen-Ion Concentration Iodoacetamide - pharmacology Kinetics Transferases
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