Evaluation of a multiplex liquid chromatography-tandem mass spectrometry method for congenital adrenal hyperplasia in pediatric patients
Multiplexed adrenal steroid measurement provides critical diagnostic information for patients with congenital adrenal hyperplasia (CAH) as confirmation of newborn screening (NBS) or as initial diagnosis. This study reports the implementation of an adrenal steroid profiling method with a turnaround t...
Gespeichert in:
Veröffentlicht in: | Clinical mass spectrometry (Del Mar, Calif.) Calif.), 2018-08, Vol.9, p.18-22 |
---|---|
Hauptverfasser: | , , , , |
Format: | Artikel |
Sprache: | eng |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
container_end_page | 22 |
---|---|
container_issue | |
container_start_page | 18 |
container_title | Clinical mass spectrometry (Del Mar, Calif.) |
container_volume | 9 |
creator | Cao, Jing Sonilal, Marilyn Roper, Stephen M Ali, Mahesheema Devaraj, Sridevi |
description | Multiplexed adrenal steroid measurement provides critical diagnostic information for patients with congenital adrenal hyperplasia (CAH) as confirmation of newborn screening (NBS) or as initial diagnosis. This study reports the implementation of an adrenal steroid profiling method with a turnaround time (TAT) of less than 24 h using liquid chromatography and tandem-mass spectrometry (LC-MS/MS). A lab-developed multiplexed LC-MS/MS assay was used to quantify levels of 11-deoxycortisol, cortisol, 17-hydroxy-progesterone (17-OHP), androstenedione, and testosterone. Intra and interassay imprecision were found to be |
doi_str_mv | 10.1016/j.clinms.2018.07.001 |
format | Article |
fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_11322746</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>3097853450</sourcerecordid><originalsourceid>FETCH-LOGICAL-c409t-348f1cbb91c93f35df2a0282ad8f34a177d0d0c4224f251cc39da51e2346a79b3</originalsourceid><addsrcrecordid>eNpVkc1q3TAQhU1oSUKSNyhFy27s6s_X1qqUkLaBQDfNWszVz7UusuRIcuh9gzx2FW4S0s3MwJw5Z-Brmk8EdwSTzdd9p7wLc-4oJmOHhw5jctKcUzZsWiHE-OHdfNZc5bzHVTFwSgQ5bc6YIIKxnp03TzeP4FcoLgYULQI0r764xZu_yLuH1WmkphRnKHGXYJkObYGgzYxmyBnlxahSt6akA6p1ihrZmJCKYWeCK-AR6GRC7dNhMWnxkB0gF9BitIOSnEJLzTah5MvmowWfzdVLv2juf9z8uf7V3v3-eXv9_a5VHIvSMj5aorZbQZRglvXaUsB0pKBHyziQYdBYY8Up5Zb2RCkmNPTEUMY3MIgtu2i-HX2XdTsbrWp2Ai-X5GZIBxnByf83wU1yFx8lIYzSgW-qw5cXhxQfVpOLnF1WxnsIJq5ZMiyGsWe8x1XKj1KVYs7J2LccguUzSLmXR5DyGaTEg6yY6tnn9z--Hb1iY_8AY3Gg5g</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>3097853450</pqid></control><display><type>article</type><title>Evaluation of a multiplex liquid chromatography-tandem mass spectrometry method for congenital adrenal hyperplasia in pediatric patients</title><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><creator>Cao, Jing ; Sonilal, Marilyn ; Roper, Stephen M ; Ali, Mahesheema ; Devaraj, Sridevi</creator><creatorcontrib>Cao, Jing ; Sonilal, Marilyn ; Roper, Stephen M ; Ali, Mahesheema ; Devaraj, Sridevi</creatorcontrib><description>Multiplexed adrenal steroid measurement provides critical diagnostic information for patients with congenital adrenal hyperplasia (CAH) as confirmation of newborn screening (NBS) or as initial diagnosis. This study reports the implementation of an adrenal steroid profiling method with a turnaround time (TAT) of less than 24 h using liquid chromatography and tandem-mass spectrometry (LC-MS/MS). A lab-developed multiplexed LC-MS/MS assay was used to quantify levels of 11-deoxycortisol, cortisol, 17-hydroxy-progesterone (17-OHP), androstenedione, and testosterone. Intra and interassay imprecision were found to be <10%. Comparison with a reference laboratory revealed <20% bias for all 5 analytes and Deming correlation coefficients >0.990. Linearity ranges were established from the lowest to upper limit calibrator concentrations with 100- to 800-fold maximum dilution. Run to run carryover was <0.1%, and acceptable matrix effect was observed (i.e., ion suppression enhancement <15%). Compared to serum samples, ethylenediaminetetraacetic acid (EDTA) and heparin plasma had large positive bias in the measurement of 11-deoxycortisol (62.2% and 60.2%, respectively) and androstenedione (43.8% and 33.2%, respectively), while cortisol, 17-OHP and testosterone showed less than 20% bias between sample types. Hemoglobin, bilirubin, or triglyceride interference decreased 11-deoxycortisol measurement in EDTA plasma (-19.3%, -25.6%, and -25.0%, respectively). Lipemia increased the measurement of testosterone by 28.9%. In summary, our multiplexed LC-MS/MS method provided highly sensitive and specific measurement of adrenal steroids. EDTA, heparin, hemolysis, icterus and/or lipemia may significantly impact assay results and should be avoided. This method provides an effective strategy for improving TAT in CAH testing and confirmation of NBS results.</description><identifier>ISSN: 2376-9998</identifier><identifier>ISSN: 2213-8005</identifier><identifier>EISSN: 2376-9998</identifier><identifier>DOI: 10.1016/j.clinms.2018.07.001</identifier><identifier>PMID: 39193353</identifier><language>eng</language><publisher>Netherlands: Elsevier</publisher><ispartof>Clinical mass spectrometry (Del Mar, Calif.), 2018-08, Vol.9, p.18-22</ispartof><rights>2018 The Association for Mass Spectrometry: Applications to the Clinical Lab (MSACL). Published by Elsevier B.V.</rights><rights>2018 The Association for Mass Spectrometry: Applications to the Clinical Lab (MSACL). Published by Elsevier B.V. 2018</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c409t-348f1cbb91c93f35df2a0282ad8f34a177d0d0c4224f251cc39da51e2346a79b3</citedby><cites>FETCH-LOGICAL-c409t-348f1cbb91c93f35df2a0282ad8f34a177d0d0c4224f251cc39da51e2346a79b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC11322746/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC11322746/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,315,729,782,786,887,27931,27932,53798,53800</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/39193353$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cao, Jing</creatorcontrib><creatorcontrib>Sonilal, Marilyn</creatorcontrib><creatorcontrib>Roper, Stephen M</creatorcontrib><creatorcontrib>Ali, Mahesheema</creatorcontrib><creatorcontrib>Devaraj, Sridevi</creatorcontrib><title>Evaluation of a multiplex liquid chromatography-tandem mass spectrometry method for congenital adrenal hyperplasia in pediatric patients</title><title>Clinical mass spectrometry (Del Mar, Calif.)</title><addtitle>Clin Mass Spectrom</addtitle><description>Multiplexed adrenal steroid measurement provides critical diagnostic information for patients with congenital adrenal hyperplasia (CAH) as confirmation of newborn screening (NBS) or as initial diagnosis. This study reports the implementation of an adrenal steroid profiling method with a turnaround time (TAT) of less than 24 h using liquid chromatography and tandem-mass spectrometry (LC-MS/MS). A lab-developed multiplexed LC-MS/MS assay was used to quantify levels of 11-deoxycortisol, cortisol, 17-hydroxy-progesterone (17-OHP), androstenedione, and testosterone. Intra and interassay imprecision were found to be <10%. Comparison with a reference laboratory revealed <20% bias for all 5 analytes and Deming correlation coefficients >0.990. Linearity ranges were established from the lowest to upper limit calibrator concentrations with 100- to 800-fold maximum dilution. Run to run carryover was <0.1%, and acceptable matrix effect was observed (i.e., ion suppression enhancement <15%). Compared to serum samples, ethylenediaminetetraacetic acid (EDTA) and heparin plasma had large positive bias in the measurement of 11-deoxycortisol (62.2% and 60.2%, respectively) and androstenedione (43.8% and 33.2%, respectively), while cortisol, 17-OHP and testosterone showed less than 20% bias between sample types. Hemoglobin, bilirubin, or triglyceride interference decreased 11-deoxycortisol measurement in EDTA plasma (-19.3%, -25.6%, and -25.0%, respectively). Lipemia increased the measurement of testosterone by 28.9%. In summary, our multiplexed LC-MS/MS method provided highly sensitive and specific measurement of adrenal steroids. EDTA, heparin, hemolysis, icterus and/or lipemia may significantly impact assay results and should be avoided. This method provides an effective strategy for improving TAT in CAH testing and confirmation of NBS results.</description><issn>2376-9998</issn><issn>2213-8005</issn><issn>2376-9998</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNpVkc1q3TAQhU1oSUKSNyhFy27s6s_X1qqUkLaBQDfNWszVz7UusuRIcuh9gzx2FW4S0s3MwJw5Z-Brmk8EdwSTzdd9p7wLc-4oJmOHhw5jctKcUzZsWiHE-OHdfNZc5bzHVTFwSgQ5bc6YIIKxnp03TzeP4FcoLgYULQI0r764xZu_yLuH1WmkphRnKHGXYJkObYGgzYxmyBnlxahSt6akA6p1ihrZmJCKYWeCK-AR6GRC7dNhMWnxkB0gF9BitIOSnEJLzTah5MvmowWfzdVLv2juf9z8uf7V3v3-eXv9_a5VHIvSMj5aorZbQZRglvXaUsB0pKBHyziQYdBYY8Up5Zb2RCkmNPTEUMY3MIgtu2i-HX2XdTsbrWp2Ai-X5GZIBxnByf83wU1yFx8lIYzSgW-qw5cXhxQfVpOLnF1WxnsIJq5ZMiyGsWe8x1XKj1KVYs7J2LccguUzSLmXR5DyGaTEg6yY6tnn9z--Hb1iY_8AY3Gg5g</recordid><startdate>20180801</startdate><enddate>20180801</enddate><creator>Cao, Jing</creator><creator>Sonilal, Marilyn</creator><creator>Roper, Stephen M</creator><creator>Ali, Mahesheema</creator><creator>Devaraj, Sridevi</creator><general>Elsevier</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20180801</creationdate><title>Evaluation of a multiplex liquid chromatography-tandem mass spectrometry method for congenital adrenal hyperplasia in pediatric patients</title><author>Cao, Jing ; Sonilal, Marilyn ; Roper, Stephen M ; Ali, Mahesheema ; Devaraj, Sridevi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c409t-348f1cbb91c93f35df2a0282ad8f34a177d0d0c4224f251cc39da51e2346a79b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><toplevel>online_resources</toplevel><creatorcontrib>Cao, Jing</creatorcontrib><creatorcontrib>Sonilal, Marilyn</creatorcontrib><creatorcontrib>Roper, Stephen M</creatorcontrib><creatorcontrib>Ali, Mahesheema</creatorcontrib><creatorcontrib>Devaraj, Sridevi</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Clinical mass spectrometry (Del Mar, Calif.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cao, Jing</au><au>Sonilal, Marilyn</au><au>Roper, Stephen M</au><au>Ali, Mahesheema</au><au>Devaraj, Sridevi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evaluation of a multiplex liquid chromatography-tandem mass spectrometry method for congenital adrenal hyperplasia in pediatric patients</atitle><jtitle>Clinical mass spectrometry (Del Mar, Calif.)</jtitle><addtitle>Clin Mass Spectrom</addtitle><date>2018-08-01</date><risdate>2018</risdate><volume>9</volume><spage>18</spage><epage>22</epage><pages>18-22</pages><issn>2376-9998</issn><issn>2213-8005</issn><eissn>2376-9998</eissn><abstract>Multiplexed adrenal steroid measurement provides critical diagnostic information for patients with congenital adrenal hyperplasia (CAH) as confirmation of newborn screening (NBS) or as initial diagnosis. This study reports the implementation of an adrenal steroid profiling method with a turnaround time (TAT) of less than 24 h using liquid chromatography and tandem-mass spectrometry (LC-MS/MS). A lab-developed multiplexed LC-MS/MS assay was used to quantify levels of 11-deoxycortisol, cortisol, 17-hydroxy-progesterone (17-OHP), androstenedione, and testosterone. Intra and interassay imprecision were found to be <10%. Comparison with a reference laboratory revealed <20% bias for all 5 analytes and Deming correlation coefficients >0.990. Linearity ranges were established from the lowest to upper limit calibrator concentrations with 100- to 800-fold maximum dilution. Run to run carryover was <0.1%, and acceptable matrix effect was observed (i.e., ion suppression enhancement <15%). Compared to serum samples, ethylenediaminetetraacetic acid (EDTA) and heparin plasma had large positive bias in the measurement of 11-deoxycortisol (62.2% and 60.2%, respectively) and androstenedione (43.8% and 33.2%, respectively), while cortisol, 17-OHP and testosterone showed less than 20% bias between sample types. Hemoglobin, bilirubin, or triglyceride interference decreased 11-deoxycortisol measurement in EDTA plasma (-19.3%, -25.6%, and -25.0%, respectively). Lipemia increased the measurement of testosterone by 28.9%. In summary, our multiplexed LC-MS/MS method provided highly sensitive and specific measurement of adrenal steroids. EDTA, heparin, hemolysis, icterus and/or lipemia may significantly impact assay results and should be avoided. This method provides an effective strategy for improving TAT in CAH testing and confirmation of NBS results.</abstract><cop>Netherlands</cop><pub>Elsevier</pub><pmid>39193353</pmid><doi>10.1016/j.clinms.2018.07.001</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
fulltext | fulltext |
identifier | ISSN: 2376-9998 |
ispartof | Clinical mass spectrometry (Del Mar, Calif.), 2018-08, Vol.9, p.18-22 |
issn | 2376-9998 2213-8005 2376-9998 |
language | eng |
recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_11322746 |
source | EZB-FREE-00999 freely available EZB journals; PubMed Central; Alma/SFX Local Collection |
title | Evaluation of a multiplex liquid chromatography-tandem mass spectrometry method for congenital adrenal hyperplasia in pediatric patients |
url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-06T12%3A13%3A11IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Evaluation%20of%20a%20multiplex%20liquid%20chromatography-tandem%20mass%20spectrometry%20method%20for%20congenital%20adrenal%20hyperplasia%20in%20pediatric%20patients&rft.jtitle=Clinical%20mass%20spectrometry%20(Del%20Mar,%20Calif.)&rft.au=Cao,%20Jing&rft.date=2018-08-01&rft.volume=9&rft.spage=18&rft.epage=22&rft.pages=18-22&rft.issn=2376-9998&rft.eissn=2376-9998&rft_id=info:doi/10.1016/j.clinms.2018.07.001&rft_dat=%3Cproquest_pubme%3E3097853450%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=3097853450&rft_id=info:pmid/39193353&rfr_iscdi=true |