fruitfly Drosophila melanogaster contains a novel charged adipokinetic-hormone-family peptide
A member of the RPCH/AKH (red-pigment-concentrating hormone/adipokinetic hormone) family of arthropod neuropeptides was identified in the fruitfly Drosophila melanogaster, and its structure was determined by automated Edman degradation and m.s. using fast-atom-bombardment ionization and a tandem hyb...
Gespeichert in:
| Veröffentlicht in: | Biochemical journal 1990-07, Vol.269 (2), p.315-320 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 320 |
|---|---|
| container_issue | 2 |
| container_start_page | 315 |
| container_title | Biochemical journal |
| container_volume | 269 |
| creator | Schaffer, M.H Noyes, B.E Slaughter, C.A Thorne, G.C Gaskell, S.J |
| description | A member of the RPCH/AKH (red-pigment-concentrating hormone/adipokinetic hormone) family of arthropod neuropeptides was identified in the fruitfly Drosophila melanogaster, and its structure was determined by automated Edman degradation and m.s. using fast-atom-bombardment ionization and a tandem hybrid instrument capable of high sensitivity. The sequence of this peptide, which we call 'DAKH', is pGlu-Leu-Thr-Phe-Ser-Pro-Asp-Trp-NH2 (where pGlu is pyroglutamic acid and Trp-NH2 is tryptophan carboxyamide). H.p.l.c. analyses of extracts of the three body segments revealed that more than 80% of the peptide is contained in the thorax. Although DAKH is typical of family members in its general structure and distribution in the animal, it is unique in containing a residue which is charged under physiological conditions. The evolutionary significance of this change is considered. |
| doi_str_mv | 10.1042/bj2690315 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_1131578</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>79944300</sourcerecordid><originalsourceid>FETCH-LOGICAL-c425t-3cef5a75f46d84f0319661cbc95a2d222b024031e732299759f63ac32b4bed023</originalsourceid><addsrcrecordid>eNqFkUFr3DAUhEVpSTdpD_kBoT4VcnD79CRb9qVQ0iYpBHpIcixClp92ldqWK3kD-fdV2GVpTzkJ3gwfoxnGTjl84iDxc_eAdQuCV6_YiksFZaOwec1WgLUsa0D-lh2n9ADAJUg4YkfIuZJCrdgvF7d-ccNT8S2GFOaNH0wx0mCmsDZpoVjYMC3GT6kwxRQeaSjsxsQ19YXp_Rx--4kWb8tNiGOYqHRm9Bk207z4nt6xN84Mid7v3xN2f_n97uK6vPl59ePi601pJVZLKSy5yqjKybpvpMsfaeua2862lcEeETtAma-kBGLbqqp1tTBWYCc76gHFCfuy487bbqTe0rREM-g5-tHEJx2M1_8rk9_odXjUnOfSVJMBH_eAGP5sKS169MnSkHugsE1ata2UAuBFY6ZxaOrnSOc7o829pkjukIaDfh5NH0bL3rN_4x-c-5Wy_mGnOxO0WUef9P0tAheACkTFhfgLa92c3g</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>15710862</pqid></control><display><type>article</type><title>fruitfly Drosophila melanogaster contains a novel charged adipokinetic-hormone-family peptide</title><source>MEDLINE</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><creator>Schaffer, M.H ; Noyes, B.E ; Slaughter, C.A ; Thorne, G.C ; Gaskell, S.J</creator><creatorcontrib>Schaffer, M.H ; Noyes, B.E ; Slaughter, C.A ; Thorne, G.C ; Gaskell, S.J</creatorcontrib><description>A member of the RPCH/AKH (red-pigment-concentrating hormone/adipokinetic hormone) family of arthropod neuropeptides was identified in the fruitfly Drosophila melanogaster, and its structure was determined by automated Edman degradation and m.s. using fast-atom-bombardment ionization and a tandem hybrid instrument capable of high sensitivity. The sequence of this peptide, which we call 'DAKH', is pGlu-Leu-Thr-Phe-Ser-Pro-Asp-Trp-NH2 (where pGlu is pyroglutamic acid and Trp-NH2 is tryptophan carboxyamide). H.p.l.c. analyses of extracts of the three body segments revealed that more than 80% of the peptide is contained in the thorax. Although DAKH is typical of family members in its general structure and distribution in the animal, it is unique in containing a residue which is charged under physiological conditions. The evolutionary significance of this change is considered.</description><identifier>ISSN: 0264-6021</identifier><identifier>EISSN: 1470-8728</identifier><identifier>DOI: 10.1042/bj2690315</identifier><identifier>PMID: 2117437</identifier><language>eng</language><publisher>England</publisher><subject>adipokinetic hormones ; Amino Acid Sequence ; amino acids ; Animals ; Biological Assay ; characterization ; Chromatography, High Pressure Liquid ; Drosophila melanogaster ; Drosophila melanogaster - analysis ; Drosophilidae ; Grasshoppers ; hormones ; Insect Hormones - isolation & purification ; Insect Hormones - pharmacology ; Mass Spectrometry ; Molecular Sequence Data ; Muscle Contraction - drug effects ; neuropeptides ; Oligopeptides - isolation & purification ; Oligopeptides - pharmacology ; peptides ; Pyrrolidonecarboxylic Acid - analogs & derivatives ; sequences ; thorax</subject><ispartof>Biochemical journal, 1990-07, Vol.269 (2), p.315-320</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c425t-3cef5a75f46d84f0319661cbc95a2d222b024031e732299759f63ac32b4bed023</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1131578/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1131578/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2117437$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Schaffer, M.H</creatorcontrib><creatorcontrib>Noyes, B.E</creatorcontrib><creatorcontrib>Slaughter, C.A</creatorcontrib><creatorcontrib>Thorne, G.C</creatorcontrib><creatorcontrib>Gaskell, S.J</creatorcontrib><title>fruitfly Drosophila melanogaster contains a novel charged adipokinetic-hormone-family peptide</title><title>Biochemical journal</title><addtitle>Biochem J</addtitle><description>A member of the RPCH/AKH (red-pigment-concentrating hormone/adipokinetic hormone) family of arthropod neuropeptides was identified in the fruitfly Drosophila melanogaster, and its structure was determined by automated Edman degradation and m.s. using fast-atom-bombardment ionization and a tandem hybrid instrument capable of high sensitivity. The sequence of this peptide, which we call 'DAKH', is pGlu-Leu-Thr-Phe-Ser-Pro-Asp-Trp-NH2 (where pGlu is pyroglutamic acid and Trp-NH2 is tryptophan carboxyamide). H.p.l.c. analyses of extracts of the three body segments revealed that more than 80% of the peptide is contained in the thorax. Although DAKH is typical of family members in its general structure and distribution in the animal, it is unique in containing a residue which is charged under physiological conditions. The evolutionary significance of this change is considered.</description><subject>adipokinetic hormones</subject><subject>Amino Acid Sequence</subject><subject>amino acids</subject><subject>Animals</subject><subject>Biological Assay</subject><subject>characterization</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Drosophila melanogaster</subject><subject>Drosophila melanogaster - analysis</subject><subject>Drosophilidae</subject><subject>Grasshoppers</subject><subject>hormones</subject><subject>Insect Hormones - isolation & purification</subject><subject>Insect Hormones - pharmacology</subject><subject>Mass Spectrometry</subject><subject>Molecular Sequence Data</subject><subject>Muscle Contraction - drug effects</subject><subject>neuropeptides</subject><subject>Oligopeptides - isolation & purification</subject><subject>Oligopeptides - pharmacology</subject><subject>peptides</subject><subject>Pyrrolidonecarboxylic Acid - analogs & derivatives</subject><subject>sequences</subject><subject>thorax</subject><issn>0264-6021</issn><issn>1470-8728</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUFr3DAUhEVpSTdpD_kBoT4VcnD79CRb9qVQ0iYpBHpIcixClp92ldqWK3kD-fdV2GVpTzkJ3gwfoxnGTjl84iDxc_eAdQuCV6_YiksFZaOwec1WgLUsa0D-lh2n9ADAJUg4YkfIuZJCrdgvF7d-ccNT8S2GFOaNH0wx0mCmsDZpoVjYMC3GT6kwxRQeaSjsxsQ19YXp_Rx--4kWb8tNiGOYqHRm9Bk207z4nt6xN84Mid7v3xN2f_n97uK6vPl59ePi601pJVZLKSy5yqjKybpvpMsfaeua2862lcEeETtAma-kBGLbqqp1tTBWYCc76gHFCfuy487bbqTe0rREM-g5-tHEJx2M1_8rk9_odXjUnOfSVJMBH_eAGP5sKS169MnSkHugsE1ata2UAuBFY6ZxaOrnSOc7o829pkjukIaDfh5NH0bL3rN_4x-c-5Wy_mGnOxO0WUef9P0tAheACkTFhfgLa92c3g</recordid><startdate>19900715</startdate><enddate>19900715</enddate><creator>Schaffer, M.H</creator><creator>Noyes, B.E</creator><creator>Slaughter, C.A</creator><creator>Thorne, G.C</creator><creator>Gaskell, S.J</creator><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7SS</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M81</scope><scope>P64</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19900715</creationdate><title>fruitfly Drosophila melanogaster contains a novel charged adipokinetic-hormone-family peptide</title><author>Schaffer, M.H ; Noyes, B.E ; Slaughter, C.A ; Thorne, G.C ; Gaskell, S.J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c425t-3cef5a75f46d84f0319661cbc95a2d222b024031e732299759f63ac32b4bed023</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>adipokinetic hormones</topic><topic>Amino Acid Sequence</topic><topic>amino acids</topic><topic>Animals</topic><topic>Biological Assay</topic><topic>characterization</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Drosophila melanogaster</topic><topic>Drosophila melanogaster - analysis</topic><topic>Drosophilidae</topic><topic>Grasshoppers</topic><topic>hormones</topic><topic>Insect Hormones - isolation & purification</topic><topic>Insect Hormones - pharmacology</topic><topic>Mass Spectrometry</topic><topic>Molecular Sequence Data</topic><topic>Muscle Contraction - drug effects</topic><topic>neuropeptides</topic><topic>Oligopeptides - isolation & purification</topic><topic>Oligopeptides - pharmacology</topic><topic>peptides</topic><topic>Pyrrolidonecarboxylic Acid - analogs & derivatives</topic><topic>sequences</topic><topic>thorax</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Schaffer, M.H</creatorcontrib><creatorcontrib>Noyes, B.E</creatorcontrib><creatorcontrib>Slaughter, C.A</creatorcontrib><creatorcontrib>Thorne, G.C</creatorcontrib><creatorcontrib>Gaskell, S.J</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biochemistry Abstracts 3</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Biochemical journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Schaffer, M.H</au><au>Noyes, B.E</au><au>Slaughter, C.A</au><au>Thorne, G.C</au><au>Gaskell, S.J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>fruitfly Drosophila melanogaster contains a novel charged adipokinetic-hormone-family peptide</atitle><jtitle>Biochemical journal</jtitle><addtitle>Biochem J</addtitle><date>1990-07-15</date><risdate>1990</risdate><volume>269</volume><issue>2</issue><spage>315</spage><epage>320</epage><pages>315-320</pages><issn>0264-6021</issn><eissn>1470-8728</eissn><abstract>A member of the RPCH/AKH (red-pigment-concentrating hormone/adipokinetic hormone) family of arthropod neuropeptides was identified in the fruitfly Drosophila melanogaster, and its structure was determined by automated Edman degradation and m.s. using fast-atom-bombardment ionization and a tandem hybrid instrument capable of high sensitivity. The sequence of this peptide, which we call 'DAKH', is pGlu-Leu-Thr-Phe-Ser-Pro-Asp-Trp-NH2 (where pGlu is pyroglutamic acid and Trp-NH2 is tryptophan carboxyamide). H.p.l.c. analyses of extracts of the three body segments revealed that more than 80% of the peptide is contained in the thorax. Although DAKH is typical of family members in its general structure and distribution in the animal, it is unique in containing a residue which is charged under physiological conditions. The evolutionary significance of this change is considered.</abstract><cop>England</cop><pmid>2117437</pmid><doi>10.1042/bj2690315</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0264-6021 |
| ispartof | Biochemical journal, 1990-07, Vol.269 (2), p.315-320 |
| issn | 0264-6021 1470-8728 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_1131578 |
| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Alma/SFX Local Collection |
| subjects | adipokinetic hormones Amino Acid Sequence amino acids Animals Biological Assay characterization Chromatography, High Pressure Liquid Drosophila melanogaster Drosophila melanogaster - analysis Drosophilidae Grasshoppers hormones Insect Hormones - isolation & purification Insect Hormones - pharmacology Mass Spectrometry Molecular Sequence Data Muscle Contraction - drug effects neuropeptides Oligopeptides - isolation & purification Oligopeptides - pharmacology peptides Pyrrolidonecarboxylic Acid - analogs & derivatives sequences thorax |
| title | fruitfly Drosophila melanogaster contains a novel charged adipokinetic-hormone-family peptide |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-29T15%3A45%3A08IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=fruitfly%20Drosophila%20melanogaster%20contains%20a%20novel%20charged%20adipokinetic-hormone-family%20peptide&rft.jtitle=Biochemical%20journal&rft.au=Schaffer,%20M.H&rft.date=1990-07-15&rft.volume=269&rft.issue=2&rft.spage=315&rft.epage=320&rft.pages=315-320&rft.issn=0264-6021&rft.eissn=1470-8728&rft_id=info:doi/10.1042/bj2690315&rft_dat=%3Cproquest_pubme%3E79944300%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=15710862&rft_id=info:pmid/2117437&rfr_iscdi=true |