New spin coated multilayer lactate biosensor for acidosis monitoring in continuous flow assisted with an electrochemical pH probe
A LOx-based electrochemical biosensor for high-level lactate determination was developed. For the construction of the biosensor, chitosan and Nafion layers were integrated by using a spin coating procedure, leading to less porous surfaces in comparison with those recorded after a drop casting proced...
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| Veröffentlicht in: | Mikrochimica acta (1966) 2024-09, Vol.191 (9), p.526, Article 526 |
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| creator | García-Guzmán, Juan José Jiménez Heras, José Manuel López-Iglesias, David González-Álvarez, Rafael Jesús Cubillana-Aguilera, Laura González Macías, Carmen Fernández Alba, Juan Jesús Palacios-Santander, José María |
| description | A LOx-based electrochemical biosensor for high-level lactate determination was developed. For the construction of the biosensor, chitosan and Nafion layers were integrated by using a spin coating procedure, leading to less porous surfaces in comparison with those recorded after a drop casting procedure. The analytical performance of the resulting biosensor for lactate determination was evaluated in batch and flow regime, displaying satisfactory results in both modes ranging from 0.5 to 20 mM concentration range for assessing the lactic acidosis. Finally, the lactate levels in raw serum samples were estimated using the biosensor developed and verified with a blood gas analyzer. Based on these results, the biosensor developed is promising for its use in healthcare environment, after its proper miniaturization. A pH probe based on common polyaniline-based electrochemical sensor was also developed to assist the biosensor for the lactic acidosis monitoring, leading to excellent results in stock solutions ranging from 6.0 to 8.0 mM and raw plasma samples. The results were confirmed by using two different approaches, blood gas analyzer and pH-meter. Consequently, the lactic acidosis monitoring could be achieved in continuous flow regime using both (bio)sensors. |
| doi_str_mv | 10.1007/s00604-024-06602-y |
| format | Article |
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For the construction of the biosensor, chitosan and Nafion layers were integrated by using a spin coating procedure, leading to less porous surfaces in comparison with those recorded after a drop casting procedure. The analytical performance of the resulting biosensor for lactate determination was evaluated in batch and flow regime, displaying satisfactory results in both modes ranging from 0.5 to 20 mM concentration range for assessing the lactic acidosis. Finally, the lactate levels in raw serum samples were estimated using the biosensor developed and verified with a blood gas analyzer. Based on these results, the biosensor developed is promising for its use in healthcare environment, after its proper miniaturization. A pH probe based on common polyaniline-based electrochemical sensor was also developed to assist the biosensor for the lactic acidosis monitoring, leading to excellent results in stock solutions ranging from 6.0 to 8.0 mM and raw plasma samples. The results were confirmed by using two different approaches, blood gas analyzer and pH-meter. Consequently, the lactic acidosis monitoring could be achieved in continuous flow regime using both (bio)sensors.</description><identifier>ISSN: 0026-3672</identifier><identifier>ISSN: 1436-5073</identifier><identifier>EISSN: 1436-5073</identifier><identifier>DOI: 10.1007/s00604-024-06602-y</identifier><identifier>PMID: 39120744</identifier><language>eng</language><publisher>Vienna: Springer Vienna</publisher><subject>Acidosis ; Acidosis, Lactic - blood ; Acidosis, Lactic - diagnosis ; Analytical Chemistry ; Aniline Compounds - chemistry ; Biosensing Techniques - instrumentation ; Biosensing Techniques - methods ; Biosensors ; Blood ; Characterization and Evaluation of Materials ; Chemical sensors ; Chemistry ; Chemistry and Materials Science ; Chitosan ; Chitosan - chemistry ; Electrochemical Techniques - instrumentation ; Electrochemical Techniques - methods ; Enzymes, Immobilized - chemistry ; Fluorocarbon Polymers - chemistry ; Gas analyzers ; Humans ; Hydrogen-Ion Concentration ; Lactic Acid - blood ; Liquid oxygen ; Microengineering ; Mixed Function Oxygenases ; Monitoring ; Multilayers ; Nanochemistry ; Nanotechnology ; Polyanilines ; Spin coating</subject><ispartof>Mikrochimica acta (1966), 2024-09, Vol.191 (9), p.526, Article 526</ispartof><rights>The Author(s) 2024</rights><rights>2024. The Author(s).</rights><rights>The Author(s) 2024. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>The Author(s) 2024 2024</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c312t-b41f82eafbd18b9838a3b70fcdaab982a13ed5034b4dff73879013e5ac69126c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00604-024-06602-y$$EPDF$$P50$$Gspringer$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00604-024-06602-y$$EHTML$$P50$$Gspringer$$Hfree_for_read</linktohtml><link.rule.ids>230,314,780,784,885,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/39120744$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>García-Guzmán, Juan José</creatorcontrib><creatorcontrib>Jiménez Heras, José Manuel</creatorcontrib><creatorcontrib>López-Iglesias, David</creatorcontrib><creatorcontrib>González-Álvarez, Rafael Jesús</creatorcontrib><creatorcontrib>Cubillana-Aguilera, Laura</creatorcontrib><creatorcontrib>González Macías, Carmen</creatorcontrib><creatorcontrib>Fernández Alba, Juan Jesús</creatorcontrib><creatorcontrib>Palacios-Santander, José María</creatorcontrib><title>New spin coated multilayer lactate biosensor for acidosis monitoring in continuous flow assisted with an electrochemical pH probe</title><title>Mikrochimica acta (1966)</title><addtitle>Microchim Acta</addtitle><addtitle>Mikrochim Acta</addtitle><description>A LOx-based electrochemical biosensor for high-level lactate determination was developed. For the construction of the biosensor, chitosan and Nafion layers were integrated by using a spin coating procedure, leading to less porous surfaces in comparison with those recorded after a drop casting procedure. The analytical performance of the resulting biosensor for lactate determination was evaluated in batch and flow regime, displaying satisfactory results in both modes ranging from 0.5 to 20 mM concentration range for assessing the lactic acidosis. Finally, the lactate levels in raw serum samples were estimated using the biosensor developed and verified with a blood gas analyzer. Based on these results, the biosensor developed is promising for its use in healthcare environment, after its proper miniaturization. A pH probe based on common polyaniline-based electrochemical sensor was also developed to assist the biosensor for the lactic acidosis monitoring, leading to excellent results in stock solutions ranging from 6.0 to 8.0 mM and raw plasma samples. The results were confirmed by using two different approaches, blood gas analyzer and pH-meter. Consequently, the lactic acidosis monitoring could be achieved in continuous flow regime using both (bio)sensors.</description><subject>Acidosis</subject><subject>Acidosis, Lactic - blood</subject><subject>Acidosis, Lactic - diagnosis</subject><subject>Analytical Chemistry</subject><subject>Aniline Compounds - chemistry</subject><subject>Biosensing Techniques - instrumentation</subject><subject>Biosensing Techniques - methods</subject><subject>Biosensors</subject><subject>Blood</subject><subject>Characterization and Evaluation of Materials</subject><subject>Chemical sensors</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Chitosan</subject><subject>Chitosan - chemistry</subject><subject>Electrochemical Techniques - instrumentation</subject><subject>Electrochemical Techniques - methods</subject><subject>Enzymes, Immobilized - chemistry</subject><subject>Fluorocarbon Polymers - chemistry</subject><subject>Gas analyzers</subject><subject>Humans</subject><subject>Hydrogen-Ion Concentration</subject><subject>Lactic Acid - blood</subject><subject>Liquid oxygen</subject><subject>Microengineering</subject><subject>Mixed Function Oxygenases</subject><subject>Monitoring</subject><subject>Multilayers</subject><subject>Nanochemistry</subject><subject>Nanotechnology</subject><subject>Polyanilines</subject><subject>Spin coating</subject><issn>0026-3672</issn><issn>1436-5073</issn><issn>1436-5073</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>EIF</sourceid><recordid>eNp9kU9vFSEUxYnR2Gf1C7gwJG7cjF5gBmZWxjS2NWl0o2vCMNBHw8AITF_e0m8ur6_WPwsXN4TL757LyUHoJYG3BEC8ywAc2gZoLc6BNvtHaENaxpsOBHuMNgCUN4wLeoKe5XwDQASn7VN0wgZCQbTtBv34bHY4Ly5gHVUxE55XX5xXe5OwV7rUHh5dzCbkmLCtpbSbYnYZzzG4EpML1_huPBQX1rhmbH3cYZUrcxDcubLFKmDjjS4p6q2ZnVYeL5d4SXE0z9ETq3w2L-7PU_Tt_OPXs8vm6svFp7MPV41mhJZmbIntqVF2nEg_Dj3rFRsFWD0pVa9UEWamDlg7tpO1gvVigNrqlObVLNfsFL0_6i7rOJtJm1CS8nJJblZpL6Ny8u-X4LbyOt5KQhjphBBV4c29QorfV5OLnF3WxnsVTPUtGQwwdAB9V9HX_6A3cU2h-jtQVRE6xipFj5ROMedk7MNvCMhDxPIYsawRy7uI5b4OvfrTx8PIr0wrwI5AXg7hmPR7939kfwLxzbYE</recordid><startdate>20240901</startdate><enddate>20240901</enddate><creator>García-Guzmán, Juan José</creator><creator>Jiménez Heras, José Manuel</creator><creator>López-Iglesias, David</creator><creator>González-Álvarez, Rafael Jesús</creator><creator>Cubillana-Aguilera, Laura</creator><creator>González Macías, Carmen</creator><creator>Fernández Alba, Juan Jesús</creator><creator>Palacios-Santander, José María</creator><general>Springer Vienna</general><general>Springer Nature B.V</general><scope>C6C</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>K9.</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20240901</creationdate><title>New spin coated multilayer lactate biosensor for acidosis monitoring in continuous flow assisted with an electrochemical pH probe</title><author>García-Guzmán, Juan José ; Jiménez Heras, José Manuel ; López-Iglesias, David ; González-Álvarez, Rafael Jesús ; Cubillana-Aguilera, Laura ; González Macías, Carmen ; Fernández Alba, Juan Jesús ; Palacios-Santander, José María</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c312t-b41f82eafbd18b9838a3b70fcdaab982a13ed5034b4dff73879013e5ac69126c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Acidosis</topic><topic>Acidosis, Lactic - blood</topic><topic>Acidosis, Lactic - diagnosis</topic><topic>Analytical Chemistry</topic><topic>Aniline Compounds - chemistry</topic><topic>Biosensing Techniques - instrumentation</topic><topic>Biosensing Techniques - methods</topic><topic>Biosensors</topic><topic>Blood</topic><topic>Characterization and Evaluation of Materials</topic><topic>Chemical sensors</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Chitosan</topic><topic>Chitosan - chemistry</topic><topic>Electrochemical Techniques - instrumentation</topic><topic>Electrochemical Techniques - methods</topic><topic>Enzymes, Immobilized - chemistry</topic><topic>Fluorocarbon Polymers - chemistry</topic><topic>Gas analyzers</topic><topic>Humans</topic><topic>Hydrogen-Ion Concentration</topic><topic>Lactic Acid - blood</topic><topic>Liquid oxygen</topic><topic>Microengineering</topic><topic>Mixed Function Oxygenases</topic><topic>Monitoring</topic><topic>Multilayers</topic><topic>Nanochemistry</topic><topic>Nanotechnology</topic><topic>Polyanilines</topic><topic>Spin coating</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>García-Guzmán, Juan José</creatorcontrib><creatorcontrib>Jiménez Heras, José Manuel</creatorcontrib><creatorcontrib>López-Iglesias, David</creatorcontrib><creatorcontrib>González-Álvarez, Rafael Jesús</creatorcontrib><creatorcontrib>Cubillana-Aguilera, Laura</creatorcontrib><creatorcontrib>González Macías, Carmen</creatorcontrib><creatorcontrib>Fernández Alba, Juan Jesús</creatorcontrib><creatorcontrib>Palacios-Santander, José María</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Mikrochimica acta (1966)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>García-Guzmán, Juan José</au><au>Jiménez Heras, José Manuel</au><au>López-Iglesias, David</au><au>González-Álvarez, Rafael Jesús</au><au>Cubillana-Aguilera, Laura</au><au>González Macías, Carmen</au><au>Fernández Alba, Juan Jesús</au><au>Palacios-Santander, José María</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>New spin coated multilayer lactate biosensor for acidosis monitoring in continuous flow assisted with an electrochemical pH probe</atitle><jtitle>Mikrochimica acta (1966)</jtitle><stitle>Microchim Acta</stitle><addtitle>Mikrochim Acta</addtitle><date>2024-09-01</date><risdate>2024</risdate><volume>191</volume><issue>9</issue><spage>526</spage><pages>526-</pages><artnum>526</artnum><issn>0026-3672</issn><issn>1436-5073</issn><eissn>1436-5073</eissn><abstract>A LOx-based electrochemical biosensor for high-level lactate determination was developed. For the construction of the biosensor, chitosan and Nafion layers were integrated by using a spin coating procedure, leading to less porous surfaces in comparison with those recorded after a drop casting procedure. The analytical performance of the resulting biosensor for lactate determination was evaluated in batch and flow regime, displaying satisfactory results in both modes ranging from 0.5 to 20 mM concentration range for assessing the lactic acidosis. Finally, the lactate levels in raw serum samples were estimated using the biosensor developed and verified with a blood gas analyzer. Based on these results, the biosensor developed is promising for its use in healthcare environment, after its proper miniaturization. A pH probe based on common polyaniline-based electrochemical sensor was also developed to assist the biosensor for the lactic acidosis monitoring, leading to excellent results in stock solutions ranging from 6.0 to 8.0 mM and raw plasma samples. The results were confirmed by using two different approaches, blood gas analyzer and pH-meter. Consequently, the lactic acidosis monitoring could be achieved in continuous flow regime using both (bio)sensors.</abstract><cop>Vienna</cop><pub>Springer Vienna</pub><pmid>39120744</pmid><doi>10.1007/s00604-024-06602-y</doi><oa>free_for_read</oa></addata></record> |
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| subjects | Acidosis Acidosis, Lactic - blood Acidosis, Lactic - diagnosis Analytical Chemistry Aniline Compounds - chemistry Biosensing Techniques - instrumentation Biosensing Techniques - methods Biosensors Blood Characterization and Evaluation of Materials Chemical sensors Chemistry Chemistry and Materials Science Chitosan Chitosan - chemistry Electrochemical Techniques - instrumentation Electrochemical Techniques - methods Enzymes, Immobilized - chemistry Fluorocarbon Polymers - chemistry Gas analyzers Humans Hydrogen-Ion Concentration Lactic Acid - blood Liquid oxygen Microengineering Mixed Function Oxygenases Monitoring Multilayers Nanochemistry Nanotechnology Polyanilines Spin coating |
| title | New spin coated multilayer lactate biosensor for acidosis monitoring in continuous flow assisted with an electrochemical pH probe |
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