The DNA strand of chimeric RNA/DNA oligonucleotides can direct gene repair/conversion activity in mammalian and plant cell-free extracts

Chimeric oligonucleotides (chimeras), consisting of RNA and DNA bases folded by complementarity into a double hairpin conformation, have been shown to alter or repair single bases in plant and animal genomes. An uninterrupted stretch of DNA bases within the chimera is known to be active in the seque...

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Veröffentlicht in:	Nucleic acids research 2000-11, Vol.28 (21), p.4332-4339
Hauptverfasser:	Gamper, H B, Parekh, H, Rice, M C, Bruner, M, Youkey, H, Kmiec, E B
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Base Sequence Cell Extracts Cell Line Cell-Free System DNA Repair - genetics DNA, Recombinant - genetics DNA, Recombinant - metabolism DNA, Single-Stranded - genetics DNA, Single-Stranded - metabolism DNA-Binding Proteins - genetics DNA-Binding Proteins - physiology Electroporation Gene Conversion - genetics Kanamycin Resistance - genetics Mice Multidrug Resistance-Associated Proteins Mutation - genetics MutS Homolog 2 Protein MutS Homolog 3 Protein Nucleic Acid Heteroduplexes - genetics Nucleic Acid Heteroduplexes - metabolism Oligonucleotides - genetics Oligonucleotides - metabolism Plant Cells Plants - genetics Plasmids - genetics Proto-Oncogene Proteins - genetics Proto-Oncogene Proteins - physiology RNA - genetics RNA - metabolism Tetracycline Resistance - genetics Transformation, Bacterial
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