The human heat-shock protein family. Expression of a novel heat-inducible HSP70 (HSP70B') and isolation of its cDNA and genomic DNA
The human heat-shock protein multigene family comprises several highly conserved proteins with structural and functional properties in common, but which vary in the extent of their inducibility in response to metabolic stress. We have isolated and characterized a novel human HSP70 cDNA, HSP70B'...
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Veröffentlicht in: | Biochemical journal 1990-04, Vol.267 (1), p.125-132 |
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description | The human heat-shock protein multigene family comprises several highly conserved proteins with structural and functional properties in common, but which vary in the extent of their inducibility in response to metabolic stress. We have isolated and characterized a novel human HSP70 cDNA, HSP70B' cDNA, and its corresponding gene sequence. HSP70B' cDNA hybrid-selected an mRNA encoding a more basic 70 kDa heat-shock protein that both the major stress-inducible HSP70 and constitutively expressed HSC70 heat-shock proteins, which in common with other heat-shock 70 kDa proteins bound ATP. The complete HSP70B' gene was sequenced and, like the major inducible HSP70 gene, is devoid of introns. The HSP70B' gene has 77% sequence similarity to the HSP70 gene and 70% similarity to HSC70 cDNA, with greatest sequence divergence towards the 3'-terminus. The HSP70B' gene represents a functional gene, as indicated by Northern-blot analysis with specific oligonucleotides, hybrid-selected translation with a specific 3' cDNA sequence and S1 nuclease protection experiments. In contrast with HSP70 mRNA, which is present at low concentrations in HeLa cells and readily induced by heat or CdCl2 treatment in both fibroblasts and HeLa cells, HSP70B' mRNA was induced only at higher temperature and showed no basal expression. The differences in patterns of induction may be due to the special features of the promoter region of the HSP70B' gene. |
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HSP70B' cDNA hybrid-selected an mRNA encoding a more basic 70 kDa heat-shock protein that both the major stress-inducible HSP70 and constitutively expressed HSC70 heat-shock proteins, which in common with other heat-shock 70 kDa proteins bound ATP. The complete HSP70B' gene was sequenced and, like the major inducible HSP70 gene, is devoid of introns. The HSP70B' gene has 77% sequence similarity to the HSP70 gene and 70% similarity to HSC70 cDNA, with greatest sequence divergence towards the 3'-terminus. The HSP70B' gene represents a functional gene, as indicated by Northern-blot analysis with specific oligonucleotides, hybrid-selected translation with a specific 3' cDNA sequence and S1 nuclease protection experiments. In contrast with HSP70 mRNA, which is present at low concentrations in HeLa cells and readily induced by heat or CdCl2 treatment in both fibroblasts and HeLa cells, HSP70B' mRNA was induced only at higher temperature and showed no basal expression. The differences in patterns of induction may be due to the special features of the promoter region of the HSP70B' gene.</description><identifier>ISSN: 0264-6021</identifier><identifier>EISSN: 1470-8728</identifier><identifier>DOI: 10.1042/bj2670125</identifier><identifier>PMID: 2327978</identifier><language>eng</language><publisher>England</publisher><subject>Adenosine Triphosphate - metabolism ; Amino Acid Sequence ; Base Sequence ; Cadmium - pharmacology ; Cadmium Chloride ; DNA - genetics ; DNA - isolation & purification ; Fibroblasts - metabolism ; Gene Expression ; genes ; Heat-Shock Proteins - genetics ; HeLa Cells ; Hot Temperature ; Humans ; Introns ; man ; Molecular Sequence Data ; Multigene Family ; Nucleic Acid Hybridization ; Promoter Regions, Genetic - genetics ; RNA, Messenger - biosynthesis</subject><ispartof>Biochemical journal, 1990-04, Vol.267 (1), p.125-132</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c467t-c2c57d8cc43a2d4c770375d22eab02add859e54f6a6ef8f78c5dbc5efe7454d13</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1131254/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1131254/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2327978$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Leung, T K</creatorcontrib><creatorcontrib>Rajendran, M Y</creatorcontrib><creatorcontrib>Monfries, C</creatorcontrib><creatorcontrib>Hall, C</creatorcontrib><creatorcontrib>Lim, L</creatorcontrib><title>The human heat-shock protein family. Expression of a novel heat-inducible HSP70 (HSP70B') and isolation of its cDNA and genomic DNA</title><title>Biochemical journal</title><addtitle>Biochem J</addtitle><description>The human heat-shock protein multigene family comprises several highly conserved proteins with structural and functional properties in common, but which vary in the extent of their inducibility in response to metabolic stress. We have isolated and characterized a novel human HSP70 cDNA, HSP70B' cDNA, and its corresponding gene sequence. HSP70B' cDNA hybrid-selected an mRNA encoding a more basic 70 kDa heat-shock protein that both the major stress-inducible HSP70 and constitutively expressed HSC70 heat-shock proteins, which in common with other heat-shock 70 kDa proteins bound ATP. The complete HSP70B' gene was sequenced and, like the major inducible HSP70 gene, is devoid of introns. The HSP70B' gene has 77% sequence similarity to the HSP70 gene and 70% similarity to HSC70 cDNA, with greatest sequence divergence towards the 3'-terminus. The HSP70B' gene represents a functional gene, as indicated by Northern-blot analysis with specific oligonucleotides, hybrid-selected translation with a specific 3' cDNA sequence and S1 nuclease protection experiments. In contrast with HSP70 mRNA, which is present at low concentrations in HeLa cells and readily induced by heat or CdCl2 treatment in both fibroblasts and HeLa cells, HSP70B' mRNA was induced only at higher temperature and showed no basal expression. The differences in patterns of induction may be due to the special features of the promoter region of the HSP70B' gene.</description><subject>Adenosine Triphosphate - metabolism</subject><subject>Amino Acid Sequence</subject><subject>Base Sequence</subject><subject>Cadmium - pharmacology</subject><subject>Cadmium Chloride</subject><subject>DNA - genetics</subject><subject>DNA - isolation & purification</subject><subject>Fibroblasts - metabolism</subject><subject>Gene Expression</subject><subject>genes</subject><subject>Heat-Shock Proteins - genetics</subject><subject>HeLa Cells</subject><subject>Hot Temperature</subject><subject>Humans</subject><subject>Introns</subject><subject>man</subject><subject>Molecular Sequence Data</subject><subject>Multigene Family</subject><subject>Nucleic Acid Hybridization</subject><subject>Promoter Regions, Genetic - genetics</subject><subject>RNA, Messenger - biosynthesis</subject><issn>0264-6021</issn><issn>1470-8728</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUtP3DAUha2Kig60i_4AJK9oWQRsx6_ZIFHeEqKVSteWY98Q08SexgmCNX-cwIxGdMXq6Op-9-joHoS-UrJPCWcH1R2TilAmPqAZ5YoUWjG9gWaESV5IwugntJXzHSGUE0420SYrmZorPUNPNw3gZuxsxA3YochNcn_xok8DhIhr24X2cR-fPix6yDmkiFONLY7pHtrlQYh-dKFqAV_8_qUI_v4qP77tYRs9Djm1dljdhSFjd3J99Lq5hZi64PA0f0Yfa9tm-LLSbfTn7PTm-KK4-nl-eXx0VTgu1VA45oTy2jleWua5U4qUSnjGwFaEWe-1mIPgtbQSal0r7YSvnIAaFBfc03IbHS59F2PVgXcQh962ZtGHzvaPJtlg_t_E0JjbdG8oLaff8slgd2XQp38j5MF0ITtoWxshjdlMLy2plvN3QSokkZqTCdxbgq5POfdQr9NQYl6qNetqJ3bnbfw1ueqyfAaVb56r</recordid><startdate>19900401</startdate><enddate>19900401</enddate><creator>Leung, T K</creator><creator>Rajendran, M Y</creator><creator>Monfries, C</creator><creator>Hall, C</creator><creator>Lim, L</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T3</scope><scope>7TM</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M81</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19900401</creationdate><title>The human heat-shock protein family. Expression of a novel heat-inducible HSP70 (HSP70B') and isolation of its cDNA and genomic DNA</title><author>Leung, T K ; Rajendran, M Y ; Monfries, C ; Hall, C ; Lim, L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c467t-c2c57d8cc43a2d4c770375d22eab02add859e54f6a6ef8f78c5dbc5efe7454d13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>Adenosine Triphosphate - metabolism</topic><topic>Amino Acid Sequence</topic><topic>Base Sequence</topic><topic>Cadmium - pharmacology</topic><topic>Cadmium Chloride</topic><topic>DNA - genetics</topic><topic>DNA - isolation & purification</topic><topic>Fibroblasts - metabolism</topic><topic>Gene Expression</topic><topic>genes</topic><topic>Heat-Shock Proteins - genetics</topic><topic>HeLa Cells</topic><topic>Hot Temperature</topic><topic>Humans</topic><topic>Introns</topic><topic>man</topic><topic>Molecular Sequence Data</topic><topic>Multigene Family</topic><topic>Nucleic Acid Hybridization</topic><topic>Promoter Regions, Genetic - genetics</topic><topic>RNA, Messenger - biosynthesis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Leung, T K</creatorcontrib><creatorcontrib>Rajendran, M Y</creatorcontrib><creatorcontrib>Monfries, C</creatorcontrib><creatorcontrib>Hall, C</creatorcontrib><creatorcontrib>Lim, L</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Human Genome Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biochemistry Abstracts 3</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Biochemical journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Leung, T K</au><au>Rajendran, M Y</au><au>Monfries, C</au><au>Hall, C</au><au>Lim, L</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The human heat-shock protein family. Expression of a novel heat-inducible HSP70 (HSP70B') and isolation of its cDNA and genomic DNA</atitle><jtitle>Biochemical journal</jtitle><addtitle>Biochem J</addtitle><date>1990-04-01</date><risdate>1990</risdate><volume>267</volume><issue>1</issue><spage>125</spage><epage>132</epage><pages>125-132</pages><issn>0264-6021</issn><eissn>1470-8728</eissn><abstract>The human heat-shock protein multigene family comprises several highly conserved proteins with structural and functional properties in common, but which vary in the extent of their inducibility in response to metabolic stress. We have isolated and characterized a novel human HSP70 cDNA, HSP70B' cDNA, and its corresponding gene sequence. HSP70B' cDNA hybrid-selected an mRNA encoding a more basic 70 kDa heat-shock protein that both the major stress-inducible HSP70 and constitutively expressed HSC70 heat-shock proteins, which in common with other heat-shock 70 kDa proteins bound ATP. The complete HSP70B' gene was sequenced and, like the major inducible HSP70 gene, is devoid of introns. The HSP70B' gene has 77% sequence similarity to the HSP70 gene and 70% similarity to HSC70 cDNA, with greatest sequence divergence towards the 3'-terminus. The HSP70B' gene represents a functional gene, as indicated by Northern-blot analysis with specific oligonucleotides, hybrid-selected translation with a specific 3' cDNA sequence and S1 nuclease protection experiments. In contrast with HSP70 mRNA, which is present at low concentrations in HeLa cells and readily induced by heat or CdCl2 treatment in both fibroblasts and HeLa cells, HSP70B' mRNA was induced only at higher temperature and showed no basal expression. The differences in patterns of induction may be due to the special features of the promoter region of the HSP70B' gene.</abstract><cop>England</cop><pmid>2327978</pmid><doi>10.1042/bj2670125</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Adenosine Triphosphate - metabolism Amino Acid Sequence Base Sequence Cadmium - pharmacology Cadmium Chloride DNA - genetics DNA - isolation & purification Fibroblasts - metabolism Gene Expression genes Heat-Shock Proteins - genetics HeLa Cells Hot Temperature Humans Introns man Molecular Sequence Data Multigene Family Nucleic Acid Hybridization Promoter Regions, Genetic - genetics RNA, Messenger - biosynthesis |
title | The human heat-shock protein family. Expression of a novel heat-inducible HSP70 (HSP70B') and isolation of its cDNA and genomic DNA |
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