The effect of bee drone brood on the motility and viability of stallion spermatozoa—an in vitro study
Bee drone brood is a beehive by-product with high hormonal activity used in natural medicine to treat male infertility. The aim of the study was to assess the effect of drone brood on stallion spermatozoa during a short-term incubation for its potential use in the equine semen extenders. Three diffe...
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| Veröffentlicht in: | In vitro cellular & developmental biology. Animal 2024-06, Vol.60 (6), p.596-608 |
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| creator | Lenický, Michal Sidor, Ewelina Dianová, Lucia Tirpák, Filip Štefunková, Nikola Dżugan, Małgorzata Halo, Marko Halo, Marko Slanina, Tomáš Urban, Iveta Bažány, Denis Greń, Agnieszka Roychoudhury, Shubhadeep Schneir, Eric Rendon Massányi, Peter |
| description | Bee drone brood is a beehive by-product with high hormonal activity used in natural medicine to treat male infertility. The aim of the study was to assess the effect of drone brood on stallion spermatozoa during a short-term incubation for its potential use in the equine semen extenders. Three different forms of fixed drone brood (frozen (FR), freeze-dried (FD), and dried extract (DE)) were used. Solutions of drone brood were compared in terms of testosterone, protein, total phenolic content, and antioxidant activity. The stallion semen was diluted with prepared drone brood solutions. The computer-assisted semen analysis (CASA) method was employed to evaluate the movement characteristics of the diluted ejaculate. To determine spermatozoa viability, the mitochondrial toxicity test (MTT) and Alamar Blue test were performed. In terms of testosterone content and antioxidant activity, a close likeness between FR and FD was found whereas DE’s composition differed notably. FR had a positive effect mainly on progressive motility, but also on sperm distance and speed parameters after 2 and 3 h of incubation. On the contrary, FD and DE acted negatively, depending on increasing dose and time. For the first time, a positive dose-dependent effect of fixed drone brood on spermatozoa survival in vitro was demonstrated.
Graphical Abstract |
| doi_str_mv | 10.1007/s11626-024-00918-y |
| format | Article |
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Animal</title><addtitle>In Vitro Cell.Dev.Biol.-Animal</addtitle><addtitle>In Vitro Cell Dev Biol Anim</addtitle><description>Bee drone brood is a beehive by-product with high hormonal activity used in natural medicine to treat male infertility. The aim of the study was to assess the effect of drone brood on stallion spermatozoa during a short-term incubation for its potential use in the equine semen extenders. Three different forms of fixed drone brood (frozen (FR), freeze-dried (FD), and dried extract (DE)) were used. Solutions of drone brood were compared in terms of testosterone, protein, total phenolic content, and antioxidant activity. The stallion semen was diluted with prepared drone brood solutions. The computer-assisted semen analysis (CASA) method was employed to evaluate the movement characteristics of the diluted ejaculate. To determine spermatozoa viability, the mitochondrial toxicity test (MTT) and Alamar Blue test were performed. In terms of testosterone content and antioxidant activity, a close likeness between FR and FD was found whereas DE’s composition differed notably. FR had a positive effect mainly on progressive motility, but also on sperm distance and speed parameters after 2 and 3 h of incubation. On the contrary, FD and DE acted negatively, depending on increasing dose and time. For the first time, a positive dose-dependent effect of fixed drone brood on spermatozoa survival in vitro was demonstrated.
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Animal</jtitle><stitle>In Vitro Cell.Dev.Biol.-Animal</stitle><addtitle>In Vitro Cell Dev Biol Anim</addtitle><date>2024-06</date><risdate>2024</risdate><volume>60</volume><issue>6</issue><spage>596</spage><epage>608</epage><pages>596-608</pages><issn>1071-2690</issn><issn>1543-706X</issn><eissn>1543-706X</eissn><abstract>Bee drone brood is a beehive by-product with high hormonal activity used in natural medicine to treat male infertility. The aim of the study was to assess the effect of drone brood on stallion spermatozoa during a short-term incubation for its potential use in the equine semen extenders. Three different forms of fixed drone brood (frozen (FR), freeze-dried (FD), and dried extract (DE)) were used. Solutions of drone brood were compared in terms of testosterone, protein, total phenolic content, and antioxidant activity. The stallion semen was diluted with prepared drone brood solutions. 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| fulltext | fulltext |
| identifier | ISSN: 1071-2690 |
| ispartof | In vitro cellular & developmental biology. Animal, 2024-06, Vol.60 (6), p.596-608 |
| issn | 1071-2690 1543-706X 1543-706X |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_11286683 |
| source | MEDLINE; SpringerLink Journals - AutoHoldings |
| subjects | Animal Genetics and Genomics Animals antioxidant activity Antioxidants Antioxidants - metabolism Antioxidants - pharmacology Beehives Bees Bees - physiology Biomedical and Life Sciences byproducts Cell Biology Cell Culture Cell Survival - drug effects Composition effects Cryopreservation Developmental Biology Dilution dose response freeze drying Horses Incubation Infertility Life Sciences Male male sterility medicine mitochondria Motility Phenolic compounds Phenols Reproductive health Semen Semen Analysis Semen Preservation - methods Semen Preservation - veterinary Sperm Sperm Motility - drug effects Spermatozoa Spermatozoa - drug effects stallions Stem Cells Testosterone Toxicity Toxicity testing Veterinary services viability |
| title | The effect of bee drone brood on the motility and viability of stallion spermatozoa—an in vitro study |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-16T19%3A21%3A19IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=The%20effect%20of%20bee%20drone%20brood%20on%20the%20motility%20and%20viability%20of%20stallion%20spermatozoa%E2%80%94an%20in%20vitro%20study&rft.jtitle=In%20vitro%20cellular%20&%20developmental%20biology.%20Animal&rft.au=Lenick%C3%BD,%20Michal&rft.date=2024-06&rft.volume=60&rft.issue=6&rft.spage=596&rft.epage=608&rft.pages=596-608&rft.issn=1071-2690&rft.eissn=1543-706X&rft_id=info:doi/10.1007/s11626-024-00918-y&rft_dat=%3Cproquest_pubme%3E3058639552%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=3086030566&rft_id=info:pmid/38772999&rfr_iscdi=true |