RNA interference-based strategies to control Botrytis cinerea infection in cultivated strawberry
Key message Gene silencing of BcDCL genes improves gray mold disease control in the cultivated strawberry. Gene silencing technology offers new opportunities to develop new formulations or new pathogen-resistant plants for reducing impacts of agricultural systems. Recent studies offered the proof of...
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| Veröffentlicht in: | Plant cell reports 2024-08, Vol.43 (8), p.201-201, Article 201 |
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| creator | Capriotti, Luca Molesini, Barbara Pandolfini, Tiziana Jin, Hailing Baraldi, Elena Cecchin, Michela Mezzetti, Bruno Sabbadini, Silvia |
| description | Key message
Gene silencing of
BcDCL
genes improves gray mold disease control in the cultivated strawberry.
Gene silencing technology offers new opportunities to develop new formulations or new pathogen-resistant plants for reducing impacts of agricultural systems. Recent studies offered the proof of concept that the symptoms of gray mold can be reduced by downregulating
Dicer-like 1
(
DCL1
) and
2
(
DCL2
) genes of
Botrytis cinerea
. In this study, we demonstrate that both solutions based on dsRNA topical treatment and
in planta
expression targeting
BcDCL1
and
BcDCL2
genes can be used to control the strawberry gray mold, the most harmful disease for different fruit crops. 50, 70 and 100 ng μL
−1
of naked
BcDCL1/2
dsRNA, sprayed on plants of
Fragaria
x
ananassa
cultivar Romina in the greenhouse, displayed significant reduction of susceptibility, compared to the negative controls, but to a lesser extent than the chemical fungicide. Three independent lines of Romina cultivar were confirmed for their stable expression of the hairpin gene construct that targets the
Bc-DCL1
and
2
sequences (hp-Bc-DCL1/2), and for the production of hp construct-derived siRNAs, by qRT-PCR and Northern blot analyses. In vitro and in vivo detached leaves, and fruits from the hp-Bc-DCL1/2 lines showed significantly enhanced tolerance to this fungal pathogen compared to the control. This decreased susceptibility was correlated to the reduced fungal biomass and the downregulation of the
Bc-DCL1
and
2
genes in
B. cinerea
. These results confirm the potential of both RNAi-based products and plants for protecting the cultivated strawberry from
B. cinerea
infection, reducing the impact of chemical pesticides on the environment and the health of consumers. |
| doi_str_mv | 10.1007/s00299-024-03288-7 |
| format | Article |
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Gene silencing of
BcDCL
genes improves gray mold disease control in the cultivated strawberry.
Gene silencing technology offers new opportunities to develop new formulations or new pathogen-resistant plants for reducing impacts of agricultural systems. Recent studies offered the proof of concept that the symptoms of gray mold can be reduced by downregulating
Dicer-like 1
(
DCL1
) and
2
(
DCL2
) genes of
Botrytis cinerea
. In this study, we demonstrate that both solutions based on dsRNA topical treatment and
in planta
expression targeting
BcDCL1
and
BcDCL2
genes can be used to control the strawberry gray mold, the most harmful disease for different fruit crops. 50, 70 and 100 ng μL
−1
of naked
BcDCL1/2
dsRNA, sprayed on plants of
Fragaria
x
ananassa
cultivar Romina in the greenhouse, displayed significant reduction of susceptibility, compared to the negative controls, but to a lesser extent than the chemical fungicide. Three independent lines of Romina cultivar were confirmed for their stable expression of the hairpin gene construct that targets the
Bc-DCL1
and
2
sequences (hp-Bc-DCL1/2), and for the production of hp construct-derived siRNAs, by qRT-PCR and Northern blot analyses. In vitro and in vivo detached leaves, and fruits from the hp-Bc-DCL1/2 lines showed significantly enhanced tolerance to this fungal pathogen compared to the control. This decreased susceptibility was correlated to the reduced fungal biomass and the downregulation of the
Bc-DCL1
and
2
genes in
B. cinerea
. These results confirm the potential of both RNAi-based products and plants for protecting the cultivated strawberry from
B. cinerea
infection, reducing the impact of chemical pesticides on the environment and the health of consumers.</description><identifier>ISSN: 0721-7714</identifier><identifier>ISSN: 1432-203X</identifier><identifier>EISSN: 1432-203X</identifier><identifier>DOI: 10.1007/s00299-024-03288-7</identifier><identifier>PMID: 39048858</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Biomedical and Life Sciences ; Biotechnology ; Botrytis - pathogenicity ; Botrytis cinerea ; Cell Biology ; Chemical pest control ; Cultivars ; Cultivation ; Disease control ; Disease Resistance - genetics ; Double-stranded RNA ; Farm buildings ; Farming systems ; Fragaria ; Fragaria - genetics ; Fragaria - microbiology ; Fragaria ananassa ; Fruit crops ; Fruit cultivation ; Fruits ; fungal biomass ; Fungi ; Fungicides ; Gene expression ; Gene Expression Regulation, Plant ; Gene silencing ; Genes ; gray mold ; greenhouses ; Grey mold ; Life Sciences ; Mold ; Northern blotting ; Original ; Original Article ; Pathogens ; Pesticides ; Plant Biochemistry ; Plant diseases ; Plant Diseases - genetics ; Plant Diseases - microbiology ; Plant Diseases - prevention & control ; Plant Proteins - genetics ; Plant Proteins - metabolism ; Plant Sciences ; RNA ; RNA Interference ; RNA, Double-Stranded - genetics ; RNA-mediated interference ; Signs and symptoms ; siRNA ; Strawberries</subject><ispartof>Plant cell reports, 2024-08, Vol.43 (8), p.201-201, Article 201</ispartof><rights>The Author(s) 2024</rights><rights>2024. The Author(s).</rights><rights>The Author(s) 2024. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>The Author(s) 2024 2024</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c389t-ec1ffcab493c514f92f7c2dbad6eb8cf662b0c6e36be6a2f57f634d8743a90ba3</cites><orcidid>0000-0001-9307-812X ; 0000-0002-2740-5708</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00299-024-03288-7$$EPDF$$P50$$Gspringer$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00299-024-03288-7$$EHTML$$P50$$Gspringer$$Hfree_for_read</linktohtml><link.rule.ids>230,314,776,780,881,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/39048858$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Capriotti, Luca</creatorcontrib><creatorcontrib>Molesini, Barbara</creatorcontrib><creatorcontrib>Pandolfini, Tiziana</creatorcontrib><creatorcontrib>Jin, Hailing</creatorcontrib><creatorcontrib>Baraldi, Elena</creatorcontrib><creatorcontrib>Cecchin, Michela</creatorcontrib><creatorcontrib>Mezzetti, Bruno</creatorcontrib><creatorcontrib>Sabbadini, Silvia</creatorcontrib><title>RNA interference-based strategies to control Botrytis cinerea infection in cultivated strawberry</title><title>Plant cell reports</title><addtitle>Plant Cell Rep</addtitle><addtitle>Plant Cell Rep</addtitle><description>Key message
Gene silencing of
BcDCL
genes improves gray mold disease control in the cultivated strawberry.
Gene silencing technology offers new opportunities to develop new formulations or new pathogen-resistant plants for reducing impacts of agricultural systems. Recent studies offered the proof of concept that the symptoms of gray mold can be reduced by downregulating
Dicer-like 1
(
DCL1
) and
2
(
DCL2
) genes of
Botrytis cinerea
. In this study, we demonstrate that both solutions based on dsRNA topical treatment and
in planta
expression targeting
BcDCL1
and
BcDCL2
genes can be used to control the strawberry gray mold, the most harmful disease for different fruit crops. 50, 70 and 100 ng μL
−1
of naked
BcDCL1/2
dsRNA, sprayed on plants of
Fragaria
x
ananassa
cultivar Romina in the greenhouse, displayed significant reduction of susceptibility, compared to the negative controls, but to a lesser extent than the chemical fungicide. Three independent lines of Romina cultivar were confirmed for their stable expression of the hairpin gene construct that targets the
Bc-DCL1
and
2
sequences (hp-Bc-DCL1/2), and for the production of hp construct-derived siRNAs, by qRT-PCR and Northern blot analyses. In vitro and in vivo detached leaves, and fruits from the hp-Bc-DCL1/2 lines showed significantly enhanced tolerance to this fungal pathogen compared to the control. This decreased susceptibility was correlated to the reduced fungal biomass and the downregulation of the
Bc-DCL1
and
2
genes in
B. cinerea
. These results confirm the potential of both RNAi-based products and plants for protecting the cultivated strawberry from
B. cinerea
infection, reducing the impact of chemical pesticides on the environment and the health of consumers.</description><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>Botrytis - pathogenicity</subject><subject>Botrytis cinerea</subject><subject>Cell Biology</subject><subject>Chemical pest control</subject><subject>Cultivars</subject><subject>Cultivation</subject><subject>Disease control</subject><subject>Disease Resistance - genetics</subject><subject>Double-stranded RNA</subject><subject>Farm buildings</subject><subject>Farming systems</subject><subject>Fragaria</subject><subject>Fragaria - genetics</subject><subject>Fragaria - microbiology</subject><subject>Fragaria ananassa</subject><subject>Fruit crops</subject><subject>Fruit cultivation</subject><subject>Fruits</subject><subject>fungal biomass</subject><subject>Fungi</subject><subject>Fungicides</subject><subject>Gene expression</subject><subject>Gene Expression Regulation, Plant</subject><subject>Gene silencing</subject><subject>Genes</subject><subject>gray mold</subject><subject>greenhouses</subject><subject>Grey mold</subject><subject>Life Sciences</subject><subject>Mold</subject><subject>Northern blotting</subject><subject>Original</subject><subject>Original Article</subject><subject>Pathogens</subject><subject>Pesticides</subject><subject>Plant Biochemistry</subject><subject>Plant diseases</subject><subject>Plant Diseases - genetics</subject><subject>Plant Diseases - microbiology</subject><subject>Plant Diseases - prevention & control</subject><subject>Plant Proteins - genetics</subject><subject>Plant Proteins - metabolism</subject><subject>Plant Sciences</subject><subject>RNA</subject><subject>RNA Interference</subject><subject>RNA, Double-Stranded - genetics</subject><subject>RNA-mediated interference</subject><subject>Signs and symptoms</subject><subject>siRNA</subject><subject>Strawberries</subject><issn>0721-7714</issn><issn>1432-203X</issn><issn>1432-203X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>EIF</sourceid><recordid>eNqFkU1rFTEYhYMo9lr9Ay5kwI2b1HxNPlZSi1qhKIiCu5jJvLmmzJ3UJFO5_960U-vHQlcJnOecvG8OQo8pOaKEqOeFEGYMJkxgwpnWWN1BGyo4w4zwz3fRhihGsVJUHKAHpZwT0kQl76MDbojQutcb9OXDu-MuzhVygAyzBzy4AmNXanYVthFKV1Pn01xzmrqXqeZ9jaXzcW64a84AvsY0t1vnl6nGy2Zb7d8HyHn_EN0Lbirw6OY8RJ9ev_p4corP3r95e3J8hj3XpmLwNATvBmG476kIhgXl2Ti4UcKgfZCSDcRL4HIA6VjoVZBcjFoJ7gwZHD9EL9bci2XYweihTewme5HjzuW9TS7aP5U5frXbdGkpZdL0VLaEZzcJOX1boFS7i8XDNLkZ0lIspz1XlEot_o8SLZTUhl-hT_9Cz9OS5_YV1xQxzAjdKLZSPqdSMoTbwSmxV2XbtWzbyrbXZVvVTE9-X_nW8rPdBvAVKE2at5B_vf2P2B_YNLfn</recordid><startdate>20240801</startdate><enddate>20240801</enddate><creator>Capriotti, Luca</creator><creator>Molesini, Barbara</creator><creator>Pandolfini, Tiziana</creator><creator>Jin, Hailing</creator><creator>Baraldi, Elena</creator><creator>Cecchin, Michela</creator><creator>Mezzetti, Bruno</creator><creator>Sabbadini, Silvia</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>C6C</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T5</scope><scope>7T7</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0001-9307-812X</orcidid><orcidid>https://orcid.org/0000-0002-2740-5708</orcidid></search><sort><creationdate>20240801</creationdate><title>RNA interference-based strategies to control Botrytis cinerea infection in cultivated strawberry</title><author>Capriotti, Luca ; Molesini, Barbara ; Pandolfini, Tiziana ; Jin, Hailing ; Baraldi, Elena ; Cecchin, Michela ; Mezzetti, Bruno ; Sabbadini, Silvia</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c389t-ec1ffcab493c514f92f7c2dbad6eb8cf662b0c6e36be6a2f57f634d8743a90ba3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Biomedical and Life Sciences</topic><topic>Biotechnology</topic><topic>Botrytis - pathogenicity</topic><topic>Botrytis cinerea</topic><topic>Cell Biology</topic><topic>Chemical pest control</topic><topic>Cultivars</topic><topic>Cultivation</topic><topic>Disease control</topic><topic>Disease Resistance - genetics</topic><topic>Double-stranded RNA</topic><topic>Farm buildings</topic><topic>Farming systems</topic><topic>Fragaria</topic><topic>Fragaria - genetics</topic><topic>Fragaria - microbiology</topic><topic>Fragaria ananassa</topic><topic>Fruit crops</topic><topic>Fruit cultivation</topic><topic>Fruits</topic><topic>fungal biomass</topic><topic>Fungi</topic><topic>Fungicides</topic><topic>Gene expression</topic><topic>Gene Expression Regulation, Plant</topic><topic>Gene silencing</topic><topic>Genes</topic><topic>gray mold</topic><topic>greenhouses</topic><topic>Grey mold</topic><topic>Life Sciences</topic><topic>Mold</topic><topic>Northern blotting</topic><topic>Original</topic><topic>Original Article</topic><topic>Pathogens</topic><topic>Pesticides</topic><topic>Plant Biochemistry</topic><topic>Plant diseases</topic><topic>Plant Diseases - genetics</topic><topic>Plant Diseases - microbiology</topic><topic>Plant Diseases - prevention & control</topic><topic>Plant Proteins - genetics</topic><topic>Plant Proteins - metabolism</topic><topic>Plant Sciences</topic><topic>RNA</topic><topic>RNA Interference</topic><topic>RNA, Double-Stranded - genetics</topic><topic>RNA-mediated interference</topic><topic>Signs and symptoms</topic><topic>siRNA</topic><topic>Strawberries</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Capriotti, Luca</creatorcontrib><creatorcontrib>Molesini, Barbara</creatorcontrib><creatorcontrib>Pandolfini, Tiziana</creatorcontrib><creatorcontrib>Jin, Hailing</creatorcontrib><creatorcontrib>Baraldi, Elena</creatorcontrib><creatorcontrib>Cecchin, Michela</creatorcontrib><creatorcontrib>Mezzetti, Bruno</creatorcontrib><creatorcontrib>Sabbadini, Silvia</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Plant cell reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Capriotti, Luca</au><au>Molesini, Barbara</au><au>Pandolfini, Tiziana</au><au>Jin, Hailing</au><au>Baraldi, Elena</au><au>Cecchin, Michela</au><au>Mezzetti, Bruno</au><au>Sabbadini, Silvia</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>RNA interference-based strategies to control Botrytis cinerea infection in cultivated strawberry</atitle><jtitle>Plant cell reports</jtitle><stitle>Plant Cell Rep</stitle><addtitle>Plant Cell Rep</addtitle><date>2024-08-01</date><risdate>2024</risdate><volume>43</volume><issue>8</issue><spage>201</spage><epage>201</epage><pages>201-201</pages><artnum>201</artnum><issn>0721-7714</issn><issn>1432-203X</issn><eissn>1432-203X</eissn><abstract>Key message
Gene silencing of
BcDCL
genes improves gray mold disease control in the cultivated strawberry.
Gene silencing technology offers new opportunities to develop new formulations or new pathogen-resistant plants for reducing impacts of agricultural systems. Recent studies offered the proof of concept that the symptoms of gray mold can be reduced by downregulating
Dicer-like 1
(
DCL1
) and
2
(
DCL2
) genes of
Botrytis cinerea
. In this study, we demonstrate that both solutions based on dsRNA topical treatment and
in planta
expression targeting
BcDCL1
and
BcDCL2
genes can be used to control the strawberry gray mold, the most harmful disease for different fruit crops. 50, 70 and 100 ng μL
−1
of naked
BcDCL1/2
dsRNA, sprayed on plants of
Fragaria
x
ananassa
cultivar Romina in the greenhouse, displayed significant reduction of susceptibility, compared to the negative controls, but to a lesser extent than the chemical fungicide. Three independent lines of Romina cultivar were confirmed for their stable expression of the hairpin gene construct that targets the
Bc-DCL1
and
2
sequences (hp-Bc-DCL1/2), and for the production of hp construct-derived siRNAs, by qRT-PCR and Northern blot analyses. In vitro and in vivo detached leaves, and fruits from the hp-Bc-DCL1/2 lines showed significantly enhanced tolerance to this fungal pathogen compared to the control. This decreased susceptibility was correlated to the reduced fungal biomass and the downregulation of the
Bc-DCL1
and
2
genes in
B. cinerea
. These results confirm the potential of both RNAi-based products and plants for protecting the cultivated strawberry from
B. cinerea
infection, reducing the impact of chemical pesticides on the environment and the health of consumers.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>39048858</pmid><doi>10.1007/s00299-024-03288-7</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0001-9307-812X</orcidid><orcidid>https://orcid.org/0000-0002-2740-5708</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0721-7714 |
| ispartof | Plant cell reports, 2024-08, Vol.43 (8), p.201-201, Article 201 |
| issn | 0721-7714 1432-203X 1432-203X |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_11269516 |
| source | MEDLINE; SpringerLink Journals |
| subjects | Biomedical and Life Sciences Biotechnology Botrytis - pathogenicity Botrytis cinerea Cell Biology Chemical pest control Cultivars Cultivation Disease control Disease Resistance - genetics Double-stranded RNA Farm buildings Farming systems Fragaria Fragaria - genetics Fragaria - microbiology Fragaria ananassa Fruit crops Fruit cultivation Fruits fungal biomass Fungi Fungicides Gene expression Gene Expression Regulation, Plant Gene silencing Genes gray mold greenhouses Grey mold Life Sciences Mold Northern blotting Original Original Article Pathogens Pesticides Plant Biochemistry Plant diseases Plant Diseases - genetics Plant Diseases - microbiology Plant Diseases - prevention & control Plant Proteins - genetics Plant Proteins - metabolism Plant Sciences RNA RNA Interference RNA, Double-Stranded - genetics RNA-mediated interference Signs and symptoms siRNA Strawberries |
| title | RNA interference-based strategies to control Botrytis cinerea infection in cultivated strawberry |
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