Cellular Traction Force Holds the Potential as a Drug Testing Readout for In Vitro Cancer Metastasis
Introduction Metastasis is responsible for 90% of cancer-related deaths worldwide. However, the potential inhibitory effects of metastasis by various anticancer drugs have been left largely unexplored. Existing preclinical models primarily focus on antiproliferative agents on the primary tumor to ha...
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| Veröffentlicht in: | Cellular and molecular bioengineering 2024-06, Vol.17 (3), p.203-217 |
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| creator | Liew, Hui Yan Liew, Xiao Hui Lin, Wei Xuan Lee, Yee Zhen Ong, Yong Sze Ogawa, Satoshi Chong, Lor Huai |
| description | Introduction
Metastasis is responsible for 90% of cancer-related deaths worldwide. However, the potential inhibitory effects of metastasis by various anticancer drugs have been left largely unexplored. Existing preclinical models primarily focus on antiproliferative agents on the primary tumor to halt the cancer growth but not in metastasis. Unlike primary tumors, metastasis requires cancer cells to exert sufficient cellular traction force through the actomyosin machinery to migrate away from the primary tumor site. Therefore, we seek to explore the potential of cellular traction force as a novel readout for screening drugs that target cancer metastasis.
Methods
In vitro models of invasive and non-invasive breast cancer were first established using MDA-MB-231 and MCF-7 cell lines, respectively. Cellular morphology was characterized, revealing spindle-like morphology in MDA-MB-231 and spherical morphology in MCF-7 cells. The baseline cellular traction force was quantified using the Traction force Microscopy technique. Cisplatin, a paradigm antimetastatic drug, and 5-Fluorouracil (5FU), a non-antimetastatic drug, were selected to evaluate the potential of cellular traction force as a drug testing readout for the in vitro cancer metastasis.
Results
MDA-MB-231 cells exhibited significantly higher baseline cellular traction force compared to MCF-7 cells. Treatment with Cisplatin, an antimetastatic drug, and 5-Fluorouracil (5FU), a non-antimetastatic drug, demonstrated distinct effects on cellular traction force in MDA-MB-231 but not in MCF-7 cells. These findings correlate with the invasive potential observed in the two models.
Conclusion
Cellular traction force emerges as a promising metric for evaluating drug efficacy in inhibiting cancer metastasis using in vitro models. This approach could enhance the screening and development of novel anti-metastatic therapies, addressing a critical gap in current anticancer drug research. |
| doi_str_mv | 10.1007/s12195-024-00811-4 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_11263313</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>3084770797</sourcerecordid><originalsourceid>FETCH-LOGICAL-c356t-f996fbb1aa57f373413ee02582b72add08e548e6c56ecd165037ac9012dd66ae3</originalsourceid><addsrcrecordid>eNp9kV1rFDEUhoMo9kP_gBcS8MabsfmYJDNXItvWFiqKrN6Gs5kz25TZpCYZof--Wbeu1QtJIIHznDfnzUvIK87eccbMSeaC96phom0Y6zhv2ifkkHdaNYpJ-XR_F-qAHOV8w5gWTLbPyYHsmaqrPyTDAqdpniDRZQJXfAz0PCaH9CJOQ6blGumXWDAUDxOFTIGepnlNl5iLD2v6FWGIc6FjTPQy0O--pEgXEBwm-gkL5Lp9fkGejTBlfPlwHpNv52fLxUVz9fnj5eLDVeOk0qUZ-16PqxUHUGaURrZcItbpO7EyAoaBdajaDrVTGt3AdXVpwPWMi2HQGlAek_c73dt5tcHB1bETTPY2-Q2kOxvB278rwV_bdfxpORdaSi6rwtsHhRR_zNWk3fjs6hdBwDhnK1nXGsNMbyr65h_0Js4pVH9bSmrNjd4Kih3lUsw54bifhjO7TdHuUrQ1RfsrRdvWptePfexbfsdWAbkDci2FNaY_b_9H9h7-kKf9</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>3083661763</pqid></control><display><type>article</type><title>Cellular Traction Force Holds the Potential as a Drug Testing Readout for In Vitro Cancer Metastasis</title><source>SpringerLink Journals - AutoHoldings</source><creator>Liew, Hui Yan ; Liew, Xiao Hui ; Lin, Wei Xuan ; Lee, Yee Zhen ; Ong, Yong Sze ; Ogawa, Satoshi ; Chong, Lor Huai</creator><creatorcontrib>Liew, Hui Yan ; Liew, Xiao Hui ; Lin, Wei Xuan ; Lee, Yee Zhen ; Ong, Yong Sze ; Ogawa, Satoshi ; Chong, Lor Huai</creatorcontrib><description>Introduction
Metastasis is responsible for 90% of cancer-related deaths worldwide. However, the potential inhibitory effects of metastasis by various anticancer drugs have been left largely unexplored. Existing preclinical models primarily focus on antiproliferative agents on the primary tumor to halt the cancer growth but not in metastasis. Unlike primary tumors, metastasis requires cancer cells to exert sufficient cellular traction force through the actomyosin machinery to migrate away from the primary tumor site. Therefore, we seek to explore the potential of cellular traction force as a novel readout for screening drugs that target cancer metastasis.
Methods
In vitro models of invasive and non-invasive breast cancer were first established using MDA-MB-231 and MCF-7 cell lines, respectively. Cellular morphology was characterized, revealing spindle-like morphology in MDA-MB-231 and spherical morphology in MCF-7 cells. The baseline cellular traction force was quantified using the Traction force Microscopy technique. Cisplatin, a paradigm antimetastatic drug, and 5-Fluorouracil (5FU), a non-antimetastatic drug, were selected to evaluate the potential of cellular traction force as a drug testing readout for the in vitro cancer metastasis.
Results
MDA-MB-231 cells exhibited significantly higher baseline cellular traction force compared to MCF-7 cells. Treatment with Cisplatin, an antimetastatic drug, and 5-Fluorouracil (5FU), a non-antimetastatic drug, demonstrated distinct effects on cellular traction force in MDA-MB-231 but not in MCF-7 cells. These findings correlate with the invasive potential observed in the two models.
Conclusion
Cellular traction force emerges as a promising metric for evaluating drug efficacy in inhibiting cancer metastasis using in vitro models. This approach could enhance the screening and development of novel anti-metastatic therapies, addressing a critical gap in current anticancer drug research.</description><identifier>ISSN: 1865-5025</identifier><identifier>EISSN: 1865-5033</identifier><identifier>DOI: 10.1007/s12195-024-00811-4</identifier><identifier>PMID: 39050509</identifier><language>eng</language><publisher>Cham: Springer International Publishing</publisher><subject>5-Fluorouracil ; Actomyosin ; Anticancer properties ; Antineoplastic drugs ; Biological and Medical Physics ; Biomaterials ; Biomedical Engineering and Bioengineering ; Biomedical Engineering/Biotechnology ; Biophysics ; Breast cancer ; Cancer ; Cell Biology ; Cell culture ; Cell morphology ; Chemotherapy ; Cisplatin ; Drug efficacy ; Drug screening ; Drug testing ; Drugs ; Engineering ; In vitro methods and tests ; Invasiveness ; Metastases ; Metastasis ; Morphology ; Original ; Original Article ; Traction force ; Tumor cell lines ; Tumors</subject><ispartof>Cellular and molecular bioengineering, 2024-06, Vol.17 (3), p.203-217</ispartof><rights>The Author(s) 2024</rights><rights>The Author(s) 2024.</rights><rights>The Author(s) 2024. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>The Author(s) 2024 2024</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c356t-f996fbb1aa57f373413ee02582b72add08e548e6c56ecd165037ac9012dd66ae3</cites><orcidid>0000-0002-1491-2524</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s12195-024-00811-4$$EPDF$$P50$$Gspringer$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s12195-024-00811-4$$EHTML$$P50$$Gspringer$$Hfree_for_read</linktohtml><link.rule.ids>230,314,780,784,885,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/39050509$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Liew, Hui Yan</creatorcontrib><creatorcontrib>Liew, Xiao Hui</creatorcontrib><creatorcontrib>Lin, Wei Xuan</creatorcontrib><creatorcontrib>Lee, Yee Zhen</creatorcontrib><creatorcontrib>Ong, Yong Sze</creatorcontrib><creatorcontrib>Ogawa, Satoshi</creatorcontrib><creatorcontrib>Chong, Lor Huai</creatorcontrib><title>Cellular Traction Force Holds the Potential as a Drug Testing Readout for In Vitro Cancer Metastasis</title><title>Cellular and molecular bioengineering</title><addtitle>Cel. Mol. Bioeng</addtitle><addtitle>Cell Mol Bioeng</addtitle><description>Introduction
Metastasis is responsible for 90% of cancer-related deaths worldwide. However, the potential inhibitory effects of metastasis by various anticancer drugs have been left largely unexplored. Existing preclinical models primarily focus on antiproliferative agents on the primary tumor to halt the cancer growth but not in metastasis. Unlike primary tumors, metastasis requires cancer cells to exert sufficient cellular traction force through the actomyosin machinery to migrate away from the primary tumor site. Therefore, we seek to explore the potential of cellular traction force as a novel readout for screening drugs that target cancer metastasis.
Methods
In vitro models of invasive and non-invasive breast cancer were first established using MDA-MB-231 and MCF-7 cell lines, respectively. Cellular morphology was characterized, revealing spindle-like morphology in MDA-MB-231 and spherical morphology in MCF-7 cells. The baseline cellular traction force was quantified using the Traction force Microscopy technique. Cisplatin, a paradigm antimetastatic drug, and 5-Fluorouracil (5FU), a non-antimetastatic drug, were selected to evaluate the potential of cellular traction force as a drug testing readout for the in vitro cancer metastasis.
Results
MDA-MB-231 cells exhibited significantly higher baseline cellular traction force compared to MCF-7 cells. Treatment with Cisplatin, an antimetastatic drug, and 5-Fluorouracil (5FU), a non-antimetastatic drug, demonstrated distinct effects on cellular traction force in MDA-MB-231 but not in MCF-7 cells. These findings correlate with the invasive potential observed in the two models.
Conclusion
Cellular traction force emerges as a promising metric for evaluating drug efficacy in inhibiting cancer metastasis using in vitro models. This approach could enhance the screening and development of novel anti-metastatic therapies, addressing a critical gap in current anticancer drug research.</description><subject>5-Fluorouracil</subject><subject>Actomyosin</subject><subject>Anticancer properties</subject><subject>Antineoplastic drugs</subject><subject>Biological and Medical Physics</subject><subject>Biomaterials</subject><subject>Biomedical Engineering and Bioengineering</subject><subject>Biomedical Engineering/Biotechnology</subject><subject>Biophysics</subject><subject>Breast cancer</subject><subject>Cancer</subject><subject>Cell Biology</subject><subject>Cell culture</subject><subject>Cell morphology</subject><subject>Chemotherapy</subject><subject>Cisplatin</subject><subject>Drug efficacy</subject><subject>Drug screening</subject><subject>Drug testing</subject><subject>Drugs</subject><subject>Engineering</subject><subject>In vitro methods and tests</subject><subject>Invasiveness</subject><subject>Metastases</subject><subject>Metastasis</subject><subject>Morphology</subject><subject>Original</subject><subject>Original Article</subject><subject>Traction force</subject><subject>Tumor cell lines</subject><subject>Tumors</subject><issn>1865-5025</issn><issn>1865-5033</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><recordid>eNp9kV1rFDEUhoMo9kP_gBcS8MabsfmYJDNXItvWFiqKrN6Gs5kz25TZpCYZof--Wbeu1QtJIIHznDfnzUvIK87eccbMSeaC96phom0Y6zhv2ifkkHdaNYpJ-XR_F-qAHOV8w5gWTLbPyYHsmaqrPyTDAqdpniDRZQJXfAz0PCaH9CJOQ6blGumXWDAUDxOFTIGepnlNl5iLD2v6FWGIc6FjTPQy0O--pEgXEBwm-gkL5Lp9fkGejTBlfPlwHpNv52fLxUVz9fnj5eLDVeOk0qUZ-16PqxUHUGaURrZcItbpO7EyAoaBdajaDrVTGt3AdXVpwPWMi2HQGlAek_c73dt5tcHB1bETTPY2-Q2kOxvB278rwV_bdfxpORdaSi6rwtsHhRR_zNWk3fjs6hdBwDhnK1nXGsNMbyr65h_0Js4pVH9bSmrNjd4Kih3lUsw54bifhjO7TdHuUrQ1RfsrRdvWptePfexbfsdWAbkDci2FNaY_b_9H9h7-kKf9</recordid><startdate>20240601</startdate><enddate>20240601</enddate><creator>Liew, Hui Yan</creator><creator>Liew, Xiao Hui</creator><creator>Lin, Wei Xuan</creator><creator>Lee, Yee Zhen</creator><creator>Ong, Yong Sze</creator><creator>Ogawa, Satoshi</creator><creator>Chong, Lor Huai</creator><general>Springer International Publishing</general><general>Springer Nature B.V</general><scope>C6C</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>JG9</scope><scope>JQ2</scope><scope>K9.</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-1491-2524</orcidid></search><sort><creationdate>20240601</creationdate><title>Cellular Traction Force Holds the Potential as a Drug Testing Readout for In Vitro Cancer Metastasis</title><author>Liew, Hui Yan ; Liew, Xiao Hui ; Lin, Wei Xuan ; Lee, Yee Zhen ; Ong, Yong Sze ; Ogawa, Satoshi ; Chong, Lor Huai</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c356t-f996fbb1aa57f373413ee02582b72add08e548e6c56ecd165037ac9012dd66ae3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>5-Fluorouracil</topic><topic>Actomyosin</topic><topic>Anticancer properties</topic><topic>Antineoplastic drugs</topic><topic>Biological and Medical Physics</topic><topic>Biomaterials</topic><topic>Biomedical Engineering and Bioengineering</topic><topic>Biomedical Engineering/Biotechnology</topic><topic>Biophysics</topic><topic>Breast cancer</topic><topic>Cancer</topic><topic>Cell Biology</topic><topic>Cell culture</topic><topic>Cell morphology</topic><topic>Chemotherapy</topic><topic>Cisplatin</topic><topic>Drug efficacy</topic><topic>Drug screening</topic><topic>Drug testing</topic><topic>Drugs</topic><topic>Engineering</topic><topic>In vitro methods and tests</topic><topic>Invasiveness</topic><topic>Metastases</topic><topic>Metastasis</topic><topic>Morphology</topic><topic>Original</topic><topic>Original Article</topic><topic>Traction force</topic><topic>Tumor cell lines</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liew, Hui Yan</creatorcontrib><creatorcontrib>Liew, Xiao Hui</creatorcontrib><creatorcontrib>Lin, Wei Xuan</creatorcontrib><creatorcontrib>Lee, Yee Zhen</creatorcontrib><creatorcontrib>Ong, Yong Sze</creatorcontrib><creatorcontrib>Ogawa, Satoshi</creatorcontrib><creatorcontrib>Chong, Lor Huai</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Cellular and molecular bioengineering</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liew, Hui Yan</au><au>Liew, Xiao Hui</au><au>Lin, Wei Xuan</au><au>Lee, Yee Zhen</au><au>Ong, Yong Sze</au><au>Ogawa, Satoshi</au><au>Chong, Lor Huai</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cellular Traction Force Holds the Potential as a Drug Testing Readout for In Vitro Cancer Metastasis</atitle><jtitle>Cellular and molecular bioengineering</jtitle><stitle>Cel. Mol. Bioeng</stitle><addtitle>Cell Mol Bioeng</addtitle><date>2024-06-01</date><risdate>2024</risdate><volume>17</volume><issue>3</issue><spage>203</spage><epage>217</epage><pages>203-217</pages><issn>1865-5025</issn><eissn>1865-5033</eissn><abstract>Introduction
Metastasis is responsible for 90% of cancer-related deaths worldwide. However, the potential inhibitory effects of metastasis by various anticancer drugs have been left largely unexplored. Existing preclinical models primarily focus on antiproliferative agents on the primary tumor to halt the cancer growth but not in metastasis. Unlike primary tumors, metastasis requires cancer cells to exert sufficient cellular traction force through the actomyosin machinery to migrate away from the primary tumor site. Therefore, we seek to explore the potential of cellular traction force as a novel readout for screening drugs that target cancer metastasis.
Methods
In vitro models of invasive and non-invasive breast cancer were first established using MDA-MB-231 and MCF-7 cell lines, respectively. Cellular morphology was characterized, revealing spindle-like morphology in MDA-MB-231 and spherical morphology in MCF-7 cells. The baseline cellular traction force was quantified using the Traction force Microscopy technique. Cisplatin, a paradigm antimetastatic drug, and 5-Fluorouracil (5FU), a non-antimetastatic drug, were selected to evaluate the potential of cellular traction force as a drug testing readout for the in vitro cancer metastasis.
Results
MDA-MB-231 cells exhibited significantly higher baseline cellular traction force compared to MCF-7 cells. Treatment with Cisplatin, an antimetastatic drug, and 5-Fluorouracil (5FU), a non-antimetastatic drug, demonstrated distinct effects on cellular traction force in MDA-MB-231 but not in MCF-7 cells. These findings correlate with the invasive potential observed in the two models.
Conclusion
Cellular traction force emerges as a promising metric for evaluating drug efficacy in inhibiting cancer metastasis using in vitro models. This approach could enhance the screening and development of novel anti-metastatic therapies, addressing a critical gap in current anticancer drug research.</abstract><cop>Cham</cop><pub>Springer International Publishing</pub><pmid>39050509</pmid><doi>10.1007/s12195-024-00811-4</doi><tpages>15</tpages><orcidid>https://orcid.org/0000-0002-1491-2524</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | 5-Fluorouracil Actomyosin Anticancer properties Antineoplastic drugs Biological and Medical Physics Biomaterials Biomedical Engineering and Bioengineering Biomedical Engineering/Biotechnology Biophysics Breast cancer Cancer Cell Biology Cell culture Cell morphology Chemotherapy Cisplatin Drug efficacy Drug screening Drug testing Drugs Engineering In vitro methods and tests Invasiveness Metastases Metastasis Morphology Original Original Article Traction force Tumor cell lines Tumors |
| title | Cellular Traction Force Holds the Potential as a Drug Testing Readout for In Vitro Cancer Metastasis |
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