Simultaneous analysis of 28 urinary VOC metabolites using ultra high performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry (UPLC-ESI/MSMS)
[Display omitted] ► We developed an UPLC-ESI/MSMS method to measure 28 urinary VOC metabolites. ► This high throughput method is precise, accurate and sensitive. ► Smokers had higher levels of urinary VOC metabolites compared to non-smokers. ► Serum cotinine correlated well with tobacco related VOC...
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| Veröffentlicht in: | Analytica chimica acta 2012-10, Vol.750, p.152-160 |
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| creator | Alwis, K. Udeni Blount, Benjamin C. Britt, April S. Patel, Dhrusti Ashley, David L. |
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► We developed an UPLC-ESI/MSMS method to measure 28 urinary VOC metabolites. ► This high throughput method is precise, accurate and sensitive. ► Smokers had higher levels of urinary VOC metabolites compared to non-smokers. ► Serum cotinine correlated well with tobacco related VOC metabolites in urine. ► Urinary VOC metabolites are effective biomarkers of tobacco-related VOC exposure.
Volatile organic compounds (VOCs) are ubiquitous in the environment, originating from many different natural and anthropogenic sources, including tobacco smoke. Long-term exposure to certain VOCs may increase the risk for cancer, birth defects, and neurocognitive impairment. Therefore, VOC exposure is an area of significant public health concern. Urinary VOC metabolites are useful biomarkers for assessing VOC exposure because of non-invasiveness of sampling and longer physiological half-lives of urinary metabolites compared with VOCs in blood and breath. We developed a method using reversed-phase ultra high performance liquid chromatography (UPLC) coupled with electrospray ionization tandem mass spectrometry (ESI/MSMS) to simultaneously quantify 28 urinary VOC metabolites as biomarkers of exposure. We describe a method that monitors metabolites of acrolein, acrylamide, acrylonitrile, benzene, 1-bromopropane, 1,3-butadiene, carbon-disulfide, crotonaldehyde, cyanide, N,N-dimethylformamide, ethylbenzene, ethylene oxide, propylene oxide, styrene, tetrachloroethylene, toluene, trichloroethylene, vinyl chloride and xylene. The method is accurate (mean accuracy for spiked matrix ranged from 84 to104%), sensitive (limit of detection ranged from 0.5 to 20ngmL−1) and precise (the relative standard deviations ranged from 2.5 to 11%). We applied this method to urine samples collected from 1203 non-smokers and 347 smokers and demonstrated that smokers have significantly elevated levels of tobacco-related biomarkers compared to non-smokers. We found significant (p |
| doi_str_mv | 10.1016/j.aca.2012.04.009 |
| format | Article |
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► We developed an UPLC-ESI/MSMS method to measure 28 urinary VOC metabolites. ► This high throughput method is precise, accurate and sensitive. ► Smokers had higher levels of urinary VOC metabolites compared to non-smokers. ► Serum cotinine correlated well with tobacco related VOC metabolites in urine. ► Urinary VOC metabolites are effective biomarkers of tobacco-related VOC exposure.
Volatile organic compounds (VOCs) are ubiquitous in the environment, originating from many different natural and anthropogenic sources, including tobacco smoke. Long-term exposure to certain VOCs may increase the risk for cancer, birth defects, and neurocognitive impairment. Therefore, VOC exposure is an area of significant public health concern. Urinary VOC metabolites are useful biomarkers for assessing VOC exposure because of non-invasiveness of sampling and longer physiological half-lives of urinary metabolites compared with VOCs in blood and breath. We developed a method using reversed-phase ultra high performance liquid chromatography (UPLC) coupled with electrospray ionization tandem mass spectrometry (ESI/MSMS) to simultaneously quantify 28 urinary VOC metabolites as biomarkers of exposure. We describe a method that monitors metabolites of acrolein, acrylamide, acrylonitrile, benzene, 1-bromopropane, 1,3-butadiene, carbon-disulfide, crotonaldehyde, cyanide, N,N-dimethylformamide, ethylbenzene, ethylene oxide, propylene oxide, styrene, tetrachloroethylene, toluene, trichloroethylene, vinyl chloride and xylene. The method is accurate (mean accuracy for spiked matrix ranged from 84 to104%), sensitive (limit of detection ranged from 0.5 to 20ngmL−1) and precise (the relative standard deviations ranged from 2.5 to 11%). We applied this method to urine samples collected from 1203 non-smokers and 347 smokers and demonstrated that smokers have significantly elevated levels of tobacco-related biomarkers compared to non-smokers. We found significant (p<0.0001) correlations between serum cotinine and most of the tobacco-related biomarkers measured. These findings confirm that this method can effectively quantify urinary VOC metabolites in a population exposed to volatile organics.</description><identifier>ISSN: 0003-2670</identifier><identifier>ISSN: 1873-4324</identifier><identifier>EISSN: 1873-4324</identifier><identifier>DOI: 10.1016/j.aca.2012.04.009</identifier><identifier>PMID: 23062436</identifier><identifier>CODEN: ACACAM</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Analytical chemistry ; Applied sciences ; Biomarkers - urine ; Chemistry ; Chromatographic methods and physical methods associated with chromatography ; Chromatography, High Pressure Liquid ; Chromatography, Reverse-Phase ; Correlation ; Cotinine - blood ; Endogenous ; Environmental Pollutants - metabolism ; Environmental Pollutants - urine ; Exact sciences and technology ; Global environmental pollution ; Other chromatographic methods ; Pollution ; Smoking ; Spectrometric and optical methods ; Spectrometry, Mass, Electrospray Ionization ; Tobacco-related ; UPLC-ESI/MSMS ; Urinary biomarkers ; VOC metabolites ; Volatile Organic Compounds - metabolism ; Volatile Organic Compounds - urine</subject><ispartof>Analytica chimica acta, 2012-10, Vol.750, p.152-160</ispartof><rights>2012</rights><rights>2014 INIST-CNRS</rights><rights>Published by Elsevier B.V.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c515t-4906e6c92334e6053def248734b2a4721498da2c9a1e5c7482c010c27791c26e3</citedby><cites>FETCH-LOGICAL-c515t-4906e6c92334e6053def248734b2a4721498da2c9a1e5c7482c010c27791c26e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.aca.2012.04.009$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,780,784,885,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=26493435$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23062436$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Alwis, K. Udeni</creatorcontrib><creatorcontrib>Blount, Benjamin C.</creatorcontrib><creatorcontrib>Britt, April S.</creatorcontrib><creatorcontrib>Patel, Dhrusti</creatorcontrib><creatorcontrib>Ashley, David L.</creatorcontrib><title>Simultaneous analysis of 28 urinary VOC metabolites using ultra high performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry (UPLC-ESI/MSMS)</title><title>Analytica chimica acta</title><addtitle>Anal Chim Acta</addtitle><description>[Display omitted]
► We developed an UPLC-ESI/MSMS method to measure 28 urinary VOC metabolites. ► This high throughput method is precise, accurate and sensitive. ► Smokers had higher levels of urinary VOC metabolites compared to non-smokers. ► Serum cotinine correlated well with tobacco related VOC metabolites in urine. ► Urinary VOC metabolites are effective biomarkers of tobacco-related VOC exposure.
Volatile organic compounds (VOCs) are ubiquitous in the environment, originating from many different natural and anthropogenic sources, including tobacco smoke. Long-term exposure to certain VOCs may increase the risk for cancer, birth defects, and neurocognitive impairment. Therefore, VOC exposure is an area of significant public health concern. Urinary VOC metabolites are useful biomarkers for assessing VOC exposure because of non-invasiveness of sampling and longer physiological half-lives of urinary metabolites compared with VOCs in blood and breath. We developed a method using reversed-phase ultra high performance liquid chromatography (UPLC) coupled with electrospray ionization tandem mass spectrometry (ESI/MSMS) to simultaneously quantify 28 urinary VOC metabolites as biomarkers of exposure. We describe a method that monitors metabolites of acrolein, acrylamide, acrylonitrile, benzene, 1-bromopropane, 1,3-butadiene, carbon-disulfide, crotonaldehyde, cyanide, N,N-dimethylformamide, ethylbenzene, ethylene oxide, propylene oxide, styrene, tetrachloroethylene, toluene, trichloroethylene, vinyl chloride and xylene. The method is accurate (mean accuracy for spiked matrix ranged from 84 to104%), sensitive (limit of detection ranged from 0.5 to 20ngmL−1) and precise (the relative standard deviations ranged from 2.5 to 11%). We applied this method to urine samples collected from 1203 non-smokers and 347 smokers and demonstrated that smokers have significantly elevated levels of tobacco-related biomarkers compared to non-smokers. We found significant (p<0.0001) correlations between serum cotinine and most of the tobacco-related biomarkers measured. These findings confirm that this method can effectively quantify urinary VOC metabolites in a population exposed to volatile organics.</description><subject>Analytical chemistry</subject><subject>Applied sciences</subject><subject>Biomarkers - urine</subject><subject>Chemistry</subject><subject>Chromatographic methods and physical methods associated with chromatography</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Chromatography, Reverse-Phase</subject><subject>Correlation</subject><subject>Cotinine - blood</subject><subject>Endogenous</subject><subject>Environmental Pollutants - metabolism</subject><subject>Environmental Pollutants - urine</subject><subject>Exact sciences and technology</subject><subject>Global environmental pollution</subject><subject>Other chromatographic methods</subject><subject>Pollution</subject><subject>Smoking</subject><subject>Spectrometric and optical methods</subject><subject>Spectrometry, Mass, Electrospray Ionization</subject><subject>Tobacco-related</subject><subject>UPLC-ESI/MSMS</subject><subject>Urinary biomarkers</subject><subject>VOC metabolites</subject><subject>Volatile Organic Compounds - metabolism</subject><subject>Volatile Organic Compounds - urine</subject><issn>0003-2670</issn><issn>1873-4324</issn><issn>1873-4324</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFks9u1DAQxiMEoqXwAFyQL0jlkK3_xdmIQ4VWBSptVaSlXC2vM9l45cSpnRQtz8UDMmWXAhc4WZZ_M_7mmy_LXjI6Y5Sps-3MWDPjlPEZlTNKq0fZMZuXIpeCy8fZMaVU5FyV9Ch7ltIWr5xR-TQ74oIqLoU6zr6vXDf50fQQpkRMb_wuuURCQ_icTNH1Ju7Il-sF6WA06-DdCIlMyfUbgmXRkNZtWjJAbELsTG-BeHc7uZrYNobOjGETzdDuiA3T4KEmX93YEvBgxxjSEM2OuNC7b2bEg6CMGjrSmZRIGn4y-C0KOL35tFzkF6vLs6vV1erN8-xJY3yCF4fzJLt5f_F58TFfXn-4XLxb5rZgxZjLiipQtuJCSFC0EDU0XKI_cs2NLDmT1bw23FaGQWFLOeeWMmp5WVbMcgXiJDvf9x2mdQe1hR4n9nqIrkNbdDBO__3Su1Zvwp1mjCsmaIkdTg8dYridII26c8mC93vDNROykqqoWPV_FJsKyQvJEGV71KKJKULzIIlRfZ8MvdWYDH2fDE2lxmRgzas_Z3mo-BUFBF4fAJOs8U3Ebbr0m1OyElIUyL3dc4DO3zmIOlkHuPnaRVyZroP7h4wfIAbZKg</recordid><startdate>20121031</startdate><enddate>20121031</enddate><creator>Alwis, K. Udeni</creator><creator>Blount, Benjamin C.</creator><creator>Britt, April S.</creator><creator>Patel, Dhrusti</creator><creator>Ashley, David L.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7ST</scope><scope>7T2</scope><scope>7TG</scope><scope>7TV</scope><scope>7U2</scope><scope>C1K</scope><scope>KL.</scope><scope>SOI</scope><scope>5PM</scope></search><sort><creationdate>20121031</creationdate><title>Simultaneous analysis of 28 urinary VOC metabolites using ultra high performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry (UPLC-ESI/MSMS)</title><author>Alwis, K. Udeni ; Blount, Benjamin C. ; Britt, April S. ; Patel, Dhrusti ; Ashley, David L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c515t-4906e6c92334e6053def248734b2a4721498da2c9a1e5c7482c010c27791c26e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Analytical chemistry</topic><topic>Applied sciences</topic><topic>Biomarkers - urine</topic><topic>Chemistry</topic><topic>Chromatographic methods and physical methods associated with chromatography</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Chromatography, Reverse-Phase</topic><topic>Correlation</topic><topic>Cotinine - blood</topic><topic>Endogenous</topic><topic>Environmental Pollutants - metabolism</topic><topic>Environmental Pollutants - urine</topic><topic>Exact sciences and technology</topic><topic>Global environmental pollution</topic><topic>Other chromatographic methods</topic><topic>Pollution</topic><topic>Smoking</topic><topic>Spectrometric and optical methods</topic><topic>Spectrometry, Mass, Electrospray Ionization</topic><topic>Tobacco-related</topic><topic>UPLC-ESI/MSMS</topic><topic>Urinary biomarkers</topic><topic>VOC metabolites</topic><topic>Volatile Organic Compounds - metabolism</topic><topic>Volatile Organic Compounds - urine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Alwis, K. Udeni</creatorcontrib><creatorcontrib>Blount, Benjamin C.</creatorcontrib><creatorcontrib>Britt, April S.</creatorcontrib><creatorcontrib>Patel, Dhrusti</creatorcontrib><creatorcontrib>Ashley, David L.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Environment Abstracts</collection><collection>Health and Safety Science Abstracts (Full archive)</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Pollution Abstracts</collection><collection>Safety Science and Risk</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Meteorological & Geoastrophysical Abstracts - Academic</collection><collection>Environment Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Analytica chimica acta</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Alwis, K. Udeni</au><au>Blount, Benjamin C.</au><au>Britt, April S.</au><au>Patel, Dhrusti</au><au>Ashley, David L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Simultaneous analysis of 28 urinary VOC metabolites using ultra high performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry (UPLC-ESI/MSMS)</atitle><jtitle>Analytica chimica acta</jtitle><addtitle>Anal Chim Acta</addtitle><date>2012-10-31</date><risdate>2012</risdate><volume>750</volume><spage>152</spage><epage>160</epage><pages>152-160</pages><issn>0003-2670</issn><issn>1873-4324</issn><eissn>1873-4324</eissn><coden>ACACAM</coden><abstract>[Display omitted]
► We developed an UPLC-ESI/MSMS method to measure 28 urinary VOC metabolites. ► This high throughput method is precise, accurate and sensitive. ► Smokers had higher levels of urinary VOC metabolites compared to non-smokers. ► Serum cotinine correlated well with tobacco related VOC metabolites in urine. ► Urinary VOC metabolites are effective biomarkers of tobacco-related VOC exposure.
Volatile organic compounds (VOCs) are ubiquitous in the environment, originating from many different natural and anthropogenic sources, including tobacco smoke. Long-term exposure to certain VOCs may increase the risk for cancer, birth defects, and neurocognitive impairment. Therefore, VOC exposure is an area of significant public health concern. Urinary VOC metabolites are useful biomarkers for assessing VOC exposure because of non-invasiveness of sampling and longer physiological half-lives of urinary metabolites compared with VOCs in blood and breath. We developed a method using reversed-phase ultra high performance liquid chromatography (UPLC) coupled with electrospray ionization tandem mass spectrometry (ESI/MSMS) to simultaneously quantify 28 urinary VOC metabolites as biomarkers of exposure. We describe a method that monitors metabolites of acrolein, acrylamide, acrylonitrile, benzene, 1-bromopropane, 1,3-butadiene, carbon-disulfide, crotonaldehyde, cyanide, N,N-dimethylformamide, ethylbenzene, ethylene oxide, propylene oxide, styrene, tetrachloroethylene, toluene, trichloroethylene, vinyl chloride and xylene. The method is accurate (mean accuracy for spiked matrix ranged from 84 to104%), sensitive (limit of detection ranged from 0.5 to 20ngmL−1) and precise (the relative standard deviations ranged from 2.5 to 11%). We applied this method to urine samples collected from 1203 non-smokers and 347 smokers and demonstrated that smokers have significantly elevated levels of tobacco-related biomarkers compared to non-smokers. We found significant (p<0.0001) correlations between serum cotinine and most of the tobacco-related biomarkers measured. These findings confirm that this method can effectively quantify urinary VOC metabolites in a population exposed to volatile organics.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>23062436</pmid><doi>10.1016/j.aca.2012.04.009</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Analytical chemistry Applied sciences Biomarkers - urine Chemistry Chromatographic methods and physical methods associated with chromatography Chromatography, High Pressure Liquid Chromatography, Reverse-Phase Correlation Cotinine - blood Endogenous Environmental Pollutants - metabolism Environmental Pollutants - urine Exact sciences and technology Global environmental pollution Other chromatographic methods Pollution Smoking Spectrometric and optical methods Spectrometry, Mass, Electrospray Ionization Tobacco-related UPLC-ESI/MSMS Urinary biomarkers VOC metabolites Volatile Organic Compounds - metabolism Volatile Organic Compounds - urine |
| title | Simultaneous analysis of 28 urinary VOC metabolites using ultra high performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry (UPLC-ESI/MSMS) |
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