Investigating the Role of Fat Mass and Obesity-Associated (FTO) Single Nucleotide Polymorphisms and Methylation in Breast Cancer
Background Fat mass and obesity-associated (FTO) protein is an mRNA demethylase enzyme essential for active genome regulation. gene codes for a protein that is part of the methylosome complex and has a regulatory role in cancer development. Some studies have shown a relationship between and cancer,...
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| Veröffentlicht in: | Curēus (Palo Alto, CA) CA), 2024-06, Vol.16 (6), p.e62851 |
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| description | Background Fat mass and obesity-associated (FTO) protein is an mRNA demethylase enzyme essential for active genome regulation.
gene codes for a protein that is part of the methylosome complex and has a regulatory role in cancer development. Some studies have shown a relationship between
and cancer, where single nucleotide polymorphisms (SNPs) may have some impact on cancer risk. The present study aimed to evaluate the risk of
polymorphisms rs9939609, rs1477196, and rs9930506; analyze the methylation status of
promoters among Mexican women with breast cancer (BC); and investigate by in silico analysis the methylation status in the region near these polymorphisms. Methods A total of 157 BC patients and 137 healthy controls were genotyped for rs9939609, rs1477196, and rs9930506
polymorphisms by TaqMan SNP Genotyping Assays. Promoter methylation was analyzed by sodium bisulfite and methylation-specific polymerase chain reaction (MSP) for 78 tissue samples. An in silico analysis using The Cancer Genome Atlas Program (TCGA) database was employed to investigate the methylation state in promoter and near polymorphism locations and its relation to survival. Results The AG genotype of
rs9930506 was associated with BC protection (P= 0.0025; adjusted OR, 0.27; 95% CI: 0.10-0.70). rs9939609 and rs1477196, according to the results of the present study, had no relation to BC. Promoter methylation status assays by MSP revealed no changes in methylation in BC or healthy tissues. Trying to know more about the methylation in promoters and near polymorphisms' relation to survival, we performed an in silico analysis. Bioinformatics analysis showed a correlation between poor survival and methylation near polymorphisms but not with methylation in the promoter region. Conclusions The AG genotype rs9930506 has a protective function against BC. Whereas high methylation near polymorphisms was related to lower survival, the hypomethylated promoter region does not impact survival. |
| doi_str_mv | 10.7759/cureus.62851 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_11260689</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>3083681566</sourcerecordid><originalsourceid>FETCH-LOGICAL-c300t-739ce4ec081fca2adef686fe84e0c4f36c1830f0d4599d8924a433e1bb2a11aa3</originalsourceid><addsrcrecordid>eNpdkU1vEzEQhi0EolXpjTOyxKVI3WKvHa_3hEpEoFJLEJSzNfHOJq527WB7K-XWn45LSlU4zUjzzDsfLyGvOTtrmln73k4Rp3Smaj3jz8hhzZWuNNfy-ZP8gByndMMY46ypWcNekgPRMskaJQ_J3YW_xZTdGrLza5o3SL-HAWno6QIyvYKUKPiOLleYXN5V5ykF6yBjR08W18t39EdpK_zXyQ4YsuuQfgvDbgxxu3Fp3DdfYd7shjIheOo8_RgRUqZz8BbjK_KihyHh8UM8Ij8Xn67nX6rL5eeL-fllZQVjuWpEa1GiZZr3FmrosFda9aglMit7oSzXgvWsk7O27XRbS5BCIF-tauAcQByRD3vd7bQasbPoc4TBbKMbIe5MAGf-rXi3MetwazivFVO6LQonDwox_JrK08zoksVhAI9hSkYwLZTmM6UK-vY_9CZM0Zf77inVKFEWLNTpnrIxpBSxf9yGM3Nvr9nba_7YW_A3Ty94hP-aKX4DFUCjSQ</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>3086763924</pqid></control><display><type>article</type><title>Investigating the Role of Fat Mass and Obesity-Associated (FTO) Single Nucleotide Polymorphisms and Methylation in Breast Cancer</title><source>PubMed Central Open Access</source><source>PubMed Central</source><creator>Hernández-Caballero, Marta Elena ; Sierra-Ramírez, Jose Alfredo ; De la Peña-Gutierrez, Marlene ; Galindo-Ramirez, Fabián</creator><creatorcontrib>Hernández-Caballero, Marta Elena ; Sierra-Ramírez, Jose Alfredo ; De la Peña-Gutierrez, Marlene ; Galindo-Ramirez, Fabián</creatorcontrib><description>Background Fat mass and obesity-associated (FTO) protein is an mRNA demethylase enzyme essential for active genome regulation.
gene codes for a protein that is part of the methylosome complex and has a regulatory role in cancer development. Some studies have shown a relationship between
and cancer, where single nucleotide polymorphisms (SNPs) may have some impact on cancer risk. The present study aimed to evaluate the risk of
polymorphisms rs9939609, rs1477196, and rs9930506; analyze the methylation status of
promoters among Mexican women with breast cancer (BC); and investigate by in silico analysis the methylation status in the region near these polymorphisms. Methods A total of 157 BC patients and 137 healthy controls were genotyped for rs9939609, rs1477196, and rs9930506
polymorphisms by TaqMan SNP Genotyping Assays. Promoter methylation was analyzed by sodium bisulfite and methylation-specific polymerase chain reaction (MSP) for 78 tissue samples. An in silico analysis using The Cancer Genome Atlas Program (TCGA) database was employed to investigate the methylation state in promoter and near polymorphism locations and its relation to survival. Results The AG genotype of
rs9930506 was associated with BC protection (P= 0.0025; adjusted OR, 0.27; 95% CI: 0.10-0.70). rs9939609 and rs1477196, according to the results of the present study, had no relation to BC. Promoter methylation status assays by MSP revealed no changes in methylation in BC or healthy tissues. Trying to know more about the methylation in promoters and near polymorphisms' relation to survival, we performed an in silico analysis. Bioinformatics analysis showed a correlation between poor survival and methylation near polymorphisms but not with methylation in the promoter region. Conclusions The AG genotype rs9930506 has a protective function against BC. Whereas high methylation near polymorphisms was related to lower survival, the hypomethylated promoter region does not impact survival.</description><identifier>ISSN: 2168-8184</identifier><identifier>EISSN: 2168-8184</identifier><identifier>DOI: 10.7759/cureus.62851</identifier><identifier>PMID: 39040764</identifier><language>eng</language><publisher>United States: Cureus Inc</publisher><subject>Alcohol use ; Breast cancer ; Cancer ; Chemotherapy ; DNA methylation ; Epigenetics ; Genes ; Genetics ; Genomes ; Oncology ; Other ; Polymorphism ; Radiation therapy ; Software ; Tumors ; Variables ; Womens health</subject><ispartof>Curēus (Palo Alto, CA), 2024-06, Vol.16 (6), p.e62851</ispartof><rights>Copyright © 2024, Hernández-Caballero et al.</rights><rights>Copyright © 2024, Hernández-Caballero et al. This work is published under https://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>Copyright © 2024, Hernández-Caballero et al. 2024 Hernández-Caballero et al.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c300t-739ce4ec081fca2adef686fe84e0c4f36c1830f0d4599d8924a433e1bb2a11aa3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC11260689/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC11260689/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,724,777,781,882,27905,27906,53772,53774</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/39040764$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hernández-Caballero, Marta Elena</creatorcontrib><creatorcontrib>Sierra-Ramírez, Jose Alfredo</creatorcontrib><creatorcontrib>De la Peña-Gutierrez, Marlene</creatorcontrib><creatorcontrib>Galindo-Ramirez, Fabián</creatorcontrib><title>Investigating the Role of Fat Mass and Obesity-Associated (FTO) Single Nucleotide Polymorphisms and Methylation in Breast Cancer</title><title>Curēus (Palo Alto, CA)</title><addtitle>Cureus</addtitle><description>Background Fat mass and obesity-associated (FTO) protein is an mRNA demethylase enzyme essential for active genome regulation.
gene codes for a protein that is part of the methylosome complex and has a regulatory role in cancer development. Some studies have shown a relationship between
and cancer, where single nucleotide polymorphisms (SNPs) may have some impact on cancer risk. The present study aimed to evaluate the risk of
polymorphisms rs9939609, rs1477196, and rs9930506; analyze the methylation status of
promoters among Mexican women with breast cancer (BC); and investigate by in silico analysis the methylation status in the region near these polymorphisms. Methods A total of 157 BC patients and 137 healthy controls were genotyped for rs9939609, rs1477196, and rs9930506
polymorphisms by TaqMan SNP Genotyping Assays. Promoter methylation was analyzed by sodium bisulfite and methylation-specific polymerase chain reaction (MSP) for 78 tissue samples. An in silico analysis using The Cancer Genome Atlas Program (TCGA) database was employed to investigate the methylation state in promoter and near polymorphism locations and its relation to survival. Results The AG genotype of
rs9930506 was associated with BC protection (P= 0.0025; adjusted OR, 0.27; 95% CI: 0.10-0.70). rs9939609 and rs1477196, according to the results of the present study, had no relation to BC. Promoter methylation status assays by MSP revealed no changes in methylation in BC or healthy tissues. Trying to know more about the methylation in promoters and near polymorphisms' relation to survival, we performed an in silico analysis. Bioinformatics analysis showed a correlation between poor survival and methylation near polymorphisms but not with methylation in the promoter region. Conclusions The AG genotype rs9930506 has a protective function against BC. Whereas high methylation near polymorphisms was related to lower survival, the hypomethylated promoter region does not impact survival.</description><subject>Alcohol use</subject><subject>Breast cancer</subject><subject>Cancer</subject><subject>Chemotherapy</subject><subject>DNA methylation</subject><subject>Epigenetics</subject><subject>Genes</subject><subject>Genetics</subject><subject>Genomes</subject><subject>Oncology</subject><subject>Other</subject><subject>Polymorphism</subject><subject>Radiation therapy</subject><subject>Software</subject><subject>Tumors</subject><subject>Variables</subject><subject>Womens health</subject><issn>2168-8184</issn><issn>2168-8184</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><recordid>eNpdkU1vEzEQhi0EolXpjTOyxKVI3WKvHa_3hEpEoFJLEJSzNfHOJq527WB7K-XWn45LSlU4zUjzzDsfLyGvOTtrmln73k4Rp3Smaj3jz8hhzZWuNNfy-ZP8gByndMMY46ypWcNekgPRMskaJQ_J3YW_xZTdGrLza5o3SL-HAWno6QIyvYKUKPiOLleYXN5V5ykF6yBjR08W18t39EdpK_zXyQ4YsuuQfgvDbgxxu3Fp3DdfYd7shjIheOo8_RgRUqZz8BbjK_KihyHh8UM8Ij8Xn67nX6rL5eeL-fllZQVjuWpEa1GiZZr3FmrosFda9aglMit7oSzXgvWsk7O27XRbS5BCIF-tauAcQByRD3vd7bQasbPoc4TBbKMbIe5MAGf-rXi3MetwazivFVO6LQonDwox_JrK08zoksVhAI9hSkYwLZTmM6UK-vY_9CZM0Zf77inVKFEWLNTpnrIxpBSxf9yGM3Nvr9nba_7YW_A3Ty94hP-aKX4DFUCjSQ</recordid><startdate>20240621</startdate><enddate>20240621</enddate><creator>Hernández-Caballero, Marta Elena</creator><creator>Sierra-Ramírez, Jose Alfredo</creator><creator>De la Peña-Gutierrez, Marlene</creator><creator>Galindo-Ramirez, Fabián</creator><general>Cureus Inc</general><general>Cureus</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20240621</creationdate><title>Investigating the Role of Fat Mass and Obesity-Associated (FTO) Single Nucleotide Polymorphisms and Methylation in Breast Cancer</title><author>Hernández-Caballero, Marta Elena ; Sierra-Ramírez, Jose Alfredo ; De la Peña-Gutierrez, Marlene ; Galindo-Ramirez, Fabián</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c300t-739ce4ec081fca2adef686fe84e0c4f36c1830f0d4599d8924a433e1bb2a11aa3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Alcohol use</topic><topic>Breast cancer</topic><topic>Cancer</topic><topic>Chemotherapy</topic><topic>DNA methylation</topic><topic>Epigenetics</topic><topic>Genes</topic><topic>Genetics</topic><topic>Genomes</topic><topic>Oncology</topic><topic>Other</topic><topic>Polymorphism</topic><topic>Radiation therapy</topic><topic>Software</topic><topic>Tumors</topic><topic>Variables</topic><topic>Womens health</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hernández-Caballero, Marta Elena</creatorcontrib><creatorcontrib>Sierra-Ramírez, Jose Alfredo</creatorcontrib><creatorcontrib>De la Peña-Gutierrez, Marlene</creatorcontrib><creatorcontrib>Galindo-Ramirez, Fabián</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Curēus (Palo Alto, CA)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hernández-Caballero, Marta Elena</au><au>Sierra-Ramírez, Jose Alfredo</au><au>De la Peña-Gutierrez, Marlene</au><au>Galindo-Ramirez, Fabián</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Investigating the Role of Fat Mass and Obesity-Associated (FTO) Single Nucleotide Polymorphisms and Methylation in Breast Cancer</atitle><jtitle>Curēus (Palo Alto, CA)</jtitle><addtitle>Cureus</addtitle><date>2024-06-21</date><risdate>2024</risdate><volume>16</volume><issue>6</issue><spage>e62851</spage><pages>e62851-</pages><issn>2168-8184</issn><eissn>2168-8184</eissn><abstract>Background Fat mass and obesity-associated (FTO) protein is an mRNA demethylase enzyme essential for active genome regulation.
gene codes for a protein that is part of the methylosome complex and has a regulatory role in cancer development. Some studies have shown a relationship between
and cancer, where single nucleotide polymorphisms (SNPs) may have some impact on cancer risk. The present study aimed to evaluate the risk of
polymorphisms rs9939609, rs1477196, and rs9930506; analyze the methylation status of
promoters among Mexican women with breast cancer (BC); and investigate by in silico analysis the methylation status in the region near these polymorphisms. Methods A total of 157 BC patients and 137 healthy controls were genotyped for rs9939609, rs1477196, and rs9930506
polymorphisms by TaqMan SNP Genotyping Assays. Promoter methylation was analyzed by sodium bisulfite and methylation-specific polymerase chain reaction (MSP) for 78 tissue samples. An in silico analysis using The Cancer Genome Atlas Program (TCGA) database was employed to investigate the methylation state in promoter and near polymorphism locations and its relation to survival. Results The AG genotype of
rs9930506 was associated with BC protection (P= 0.0025; adjusted OR, 0.27; 95% CI: 0.10-0.70). rs9939609 and rs1477196, according to the results of the present study, had no relation to BC. Promoter methylation status assays by MSP revealed no changes in methylation in BC or healthy tissues. Trying to know more about the methylation in promoters and near polymorphisms' relation to survival, we performed an in silico analysis. Bioinformatics analysis showed a correlation between poor survival and methylation near polymorphisms but not with methylation in the promoter region. Conclusions The AG genotype rs9930506 has a protective function against BC. Whereas high methylation near polymorphisms was related to lower survival, the hypomethylated promoter region does not impact survival.</abstract><cop>United States</cop><pub>Cureus Inc</pub><pmid>39040764</pmid><doi>10.7759/cureus.62851</doi><oa>free_for_read</oa></addata></record> |
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| subjects | Alcohol use Breast cancer Cancer Chemotherapy DNA methylation Epigenetics Genes Genetics Genomes Oncology Other Polymorphism Radiation therapy Software Tumors Variables Womens health |
| title | Investigating the Role of Fat Mass and Obesity-Associated (FTO) Single Nucleotide Polymorphisms and Methylation in Breast Cancer |
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