Metabolic versatility of anaerobic sludge towards platform chemical production from waste glycerol
Waste glycerol is produced in excess by several industries, such as during biodiesel production. In this work, the metabolic versatility of anaerobic sludge was explored towards waste glycerol valorization. By applying different environmental (methanogenic and sulfate-reducing) conditions, three dis...
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| Veröffentlicht in: | Applied microbiology and biotechnology 2024-12, Vol.108 (1), p.419, Article 419 |
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| creator | Magalhães, Carla P. Alves, Joana I. Duber, Anna Oleskowicz-Popiel, Piotr Stams, Alfons J. M. Cavaleiro, Ana J. |
| description | Waste glycerol is produced in excess by several industries, such as during biodiesel production. In this work, the metabolic versatility of anaerobic sludge was explored towards waste glycerol valorization. By applying different environmental (methanogenic and sulfate-reducing) conditions, three distinct microbial cultures were obtained from the same inoculum (anaerobic granular sludge), with high microbial specialization, within three different phyla (
Thermodesulfobacteriota
,
Euryarchaeota
and
Pseudomonadota
). The cultures are capable of glycerol conversion through different pathways: (i) glycerol conversion to methane by a bacterium closely related to
Solidesulfovibrio alcoholivorans
(99.8% 16S rRNA gene identity), in syntrophic relationship with
Methanofollis liminatans
(98.8% identity), (ii) fermentation to propionate by
Propionivibrio pelophilus
strain asp66 (98.6% identity), with a propionate yield of 0.88 mmol mmol
−1
(0.71 mg mg
−1
) and a propionate purity of 80–97% and (iii) acetate production coupled to sulfate reduction by
Desulfolutivibrio sulfoxidireducens
(98.3% identity). In conclusion, starting from the same inoculum, we could drive the metabolic and functional potential of the microbiota towards the formation of several valuable products that can be used in industrial applications or as energy carriers.
Key points
Versatility of anaerobic cultures was explored for waste glycerol valorization
Different environmental conditions lead to metabolic specialization
Biocommodities such as propionate, acetate and methane were produced |
| doi_str_mv | 10.1007/s00253-024-13248-6 |
| format | Article |
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Thermodesulfobacteriota
,
Euryarchaeota
and
Pseudomonadota
). The cultures are capable of glycerol conversion through different pathways: (i) glycerol conversion to methane by a bacterium closely related to
Solidesulfovibrio alcoholivorans
(99.8% 16S rRNA gene identity), in syntrophic relationship with
Methanofollis liminatans
(98.8% identity), (ii) fermentation to propionate by
Propionivibrio pelophilus
strain asp66 (98.6% identity), with a propionate yield of 0.88 mmol mmol
−1
(0.71 mg mg
−1
) and a propionate purity of 80–97% and (iii) acetate production coupled to sulfate reduction by
Desulfolutivibrio sulfoxidireducens
(98.3% identity). In conclusion, starting from the same inoculum, we could drive the metabolic and functional potential of the microbiota towards the formation of several valuable products that can be used in industrial applications or as energy carriers.
Key points
Versatility of anaerobic cultures was explored for waste glycerol valorization
Different environmental conditions lead to metabolic specialization
Biocommodities such as propionate, acetate and methane were produced</description><identifier>ISSN: 0175-7598</identifier><identifier>ISSN: 1432-0614</identifier><identifier>EISSN: 1432-0614</identifier><identifier>DOI: 10.1007/s00253-024-13248-6</identifier><identifier>PMID: 39012392</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Acetates - metabolism ; Anaerobic conditions ; Anaerobic microorganisms ; Anaerobiosis ; Bacteria - classification ; Bacteria - genetics ; Bacteria - metabolism ; Biodiesel fuels ; Biofuels ; Biomedical and Life Sciences ; Biotechnology ; Environmental Biotechnology ; Environmental conditions ; Fermentation ; Glycerol ; Glycerol - metabolism ; Industrial applications ; Inoculum ; Life Sciences ; Metabolism ; Methane ; Methane - metabolism ; Microbial Genetics and Genomics ; Microbiology ; Microbiota ; Microorganisms ; Phylogeny ; Propionates - metabolism ; Propionic acid ; RNA, Ribosomal, 16S - genetics ; rRNA 16S ; Sewage - microbiology ; Sludge ; Sulfate reduction ; Sulfates ; Sulfates - metabolism ; Versatility</subject><ispartof>Applied microbiology and biotechnology, 2024-12, Vol.108 (1), p.419, Article 419</ispartof><rights>The Author(s) 2024</rights><rights>2024. The Author(s).</rights><rights>The Author(s) 2024. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>The Author(s) 2024 2024</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c337t-af031b179cc41bc26ecf83b412df64c93a7c8a8ae3503c3526588e56636787e53</cites><orcidid>0000-0002-7999-4620</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00253-024-13248-6$$EPDF$$P50$$Gspringer$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://doi.org/10.1007/s00253-024-13248-6$$EHTML$$P50$$Gspringer$$Hfree_for_read</linktohtml><link.rule.ids>230,314,780,784,885,27924,27925,41120,41488,42189,42557,51319,51576</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/39012392$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Magalhães, Carla P.</creatorcontrib><creatorcontrib>Alves, Joana I.</creatorcontrib><creatorcontrib>Duber, Anna</creatorcontrib><creatorcontrib>Oleskowicz-Popiel, Piotr</creatorcontrib><creatorcontrib>Stams, Alfons J. M.</creatorcontrib><creatorcontrib>Cavaleiro, Ana J.</creatorcontrib><title>Metabolic versatility of anaerobic sludge towards platform chemical production from waste glycerol</title><title>Applied microbiology and biotechnology</title><addtitle>Appl Microbiol Biotechnol</addtitle><addtitle>Appl Microbiol Biotechnol</addtitle><description>Waste glycerol is produced in excess by several industries, such as during biodiesel production. In this work, the metabolic versatility of anaerobic sludge was explored towards waste glycerol valorization. By applying different environmental (methanogenic and sulfate-reducing) conditions, three distinct microbial cultures were obtained from the same inoculum (anaerobic granular sludge), with high microbial specialization, within three different phyla (
Thermodesulfobacteriota
,
Euryarchaeota
and
Pseudomonadota
). The cultures are capable of glycerol conversion through different pathways: (i) glycerol conversion to methane by a bacterium closely related to
Solidesulfovibrio alcoholivorans
(99.8% 16S rRNA gene identity), in syntrophic relationship with
Methanofollis liminatans
(98.8% identity), (ii) fermentation to propionate by
Propionivibrio pelophilus
strain asp66 (98.6% identity), with a propionate yield of 0.88 mmol mmol
−1
(0.71 mg mg
−1
) and a propionate purity of 80–97% and (iii) acetate production coupled to sulfate reduction by
Desulfolutivibrio sulfoxidireducens
(98.3% identity). In conclusion, starting from the same inoculum, we could drive the metabolic and functional potential of the microbiota towards the formation of several valuable products that can be used in industrial applications or as energy carriers.
Key points
Versatility of anaerobic cultures was explored for waste glycerol valorization
Different environmental conditions lead to metabolic specialization
Biocommodities such as propionate, acetate and methane were produced</description><subject>Acetates - metabolism</subject><subject>Anaerobic conditions</subject><subject>Anaerobic microorganisms</subject><subject>Anaerobiosis</subject><subject>Bacteria - classification</subject><subject>Bacteria - genetics</subject><subject>Bacteria - metabolism</subject><subject>Biodiesel fuels</subject><subject>Biofuels</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>Environmental Biotechnology</subject><subject>Environmental conditions</subject><subject>Fermentation</subject><subject>Glycerol</subject><subject>Glycerol - metabolism</subject><subject>Industrial applications</subject><subject>Inoculum</subject><subject>Life Sciences</subject><subject>Metabolism</subject><subject>Methane</subject><subject>Methane - metabolism</subject><subject>Microbial Genetics and Genomics</subject><subject>Microbiology</subject><subject>Microbiota</subject><subject>Microorganisms</subject><subject>Phylogeny</subject><subject>Propionates - metabolism</subject><subject>Propionic acid</subject><subject>RNA, Ribosomal, 16S - genetics</subject><subject>rRNA 16S</subject><subject>Sewage - microbiology</subject><subject>Sludge</subject><subject>Sulfate reduction</subject><subject>Sulfates</subject><subject>Sulfates - metabolism</subject><subject>Versatility</subject><issn>0175-7598</issn><issn>1432-0614</issn><issn>1432-0614</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>EIF</sourceid><recordid>eNp9kU1vFSEUhonR2NvqH3BhSNy4GeUbZmVMo9akjRtdE4Y5c0vDDFdg2tx_L-2t9WPRFSTvw8M5eRF6Rck7Soh-XwhhkneEiY5yJkynnqANFZx1RFHxFG0I1bLTsjdH6LiUK0IoM0o9R0e8b1fesw0aLqC6IcXg8TXk4mqIoe5xmrBbHOQ0tKDEddwCrunG5bHgXXR1SnnG_hLm4F3Eu5zG1deQFjzlNOMbVyrgbdz7Zogv0LPJxQIv788T9OPzp--nZ935ty9fTz-ed55zXTs3EU4HqnvvBR08U-AnwwdB2Tgp4XvutDfOOOCScM8lU9IYkEpxpY0GyU_Qh4N3tw4zjB6Wml20uxxml_c2uWD_TZZwabfp2lLKJGOUNMPbe0NOP1co1c6heIjRLZDWYjkxlPW9kKqhb_5Dr9Kal7bfHSV0rxhrFDtQPqdSMkwP01Bibzu0hw5t69DedWhv1a__3uPhye_SGsAPQGnRsoX85-9HtL8AAKCpGA</recordid><startdate>20241201</startdate><enddate>20241201</enddate><creator>Magalhães, Carla P.</creator><creator>Alves, Joana I.</creator><creator>Duber, Anna</creator><creator>Oleskowicz-Popiel, Piotr</creator><creator>Stams, Alfons J. M.</creator><creator>Cavaleiro, Ana J.</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>C6C</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-7999-4620</orcidid></search><sort><creationdate>20241201</creationdate><title>Metabolic versatility of anaerobic sludge towards platform chemical production from waste glycerol</title><author>Magalhães, Carla P. ; Alves, Joana I. ; Duber, Anna ; Oleskowicz-Popiel, Piotr ; Stams, Alfons J. M. ; Cavaleiro, Ana J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c337t-af031b179cc41bc26ecf83b412df64c93a7c8a8ae3503c3526588e56636787e53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Acetates - metabolism</topic><topic>Anaerobic conditions</topic><topic>Anaerobic microorganisms</topic><topic>Anaerobiosis</topic><topic>Bacteria - classification</topic><topic>Bacteria - genetics</topic><topic>Bacteria - metabolism</topic><topic>Biodiesel fuels</topic><topic>Biofuels</topic><topic>Biomedical and Life Sciences</topic><topic>Biotechnology</topic><topic>Environmental Biotechnology</topic><topic>Environmental conditions</topic><topic>Fermentation</topic><topic>Glycerol</topic><topic>Glycerol - metabolism</topic><topic>Industrial applications</topic><topic>Inoculum</topic><topic>Life Sciences</topic><topic>Metabolism</topic><topic>Methane</topic><topic>Methane - metabolism</topic><topic>Microbial Genetics and Genomics</topic><topic>Microbiology</topic><topic>Microbiota</topic><topic>Microorganisms</topic><topic>Phylogeny</topic><topic>Propionates - metabolism</topic><topic>Propionic acid</topic><topic>RNA, Ribosomal, 16S - genetics</topic><topic>rRNA 16S</topic><topic>Sewage - microbiology</topic><topic>Sludge</topic><topic>Sulfate reduction</topic><topic>Sulfates</topic><topic>Sulfates - metabolism</topic><topic>Versatility</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Magalhães, Carla P.</creatorcontrib><creatorcontrib>Alves, Joana I.</creatorcontrib><creatorcontrib>Duber, Anna</creatorcontrib><creatorcontrib>Oleskowicz-Popiel, Piotr</creatorcontrib><creatorcontrib>Stams, Alfons J. M.</creatorcontrib><creatorcontrib>Cavaleiro, Ana J.</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Applied microbiology and biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Magalhães, Carla P.</au><au>Alves, Joana I.</au><au>Duber, Anna</au><au>Oleskowicz-Popiel, Piotr</au><au>Stams, Alfons J. M.</au><au>Cavaleiro, Ana J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Metabolic versatility of anaerobic sludge towards platform chemical production from waste glycerol</atitle><jtitle>Applied microbiology and biotechnology</jtitle><stitle>Appl Microbiol Biotechnol</stitle><addtitle>Appl Microbiol Biotechnol</addtitle><date>2024-12-01</date><risdate>2024</risdate><volume>108</volume><issue>1</issue><spage>419</spage><pages>419-</pages><artnum>419</artnum><issn>0175-7598</issn><issn>1432-0614</issn><eissn>1432-0614</eissn><abstract>Waste glycerol is produced in excess by several industries, such as during biodiesel production. In this work, the metabolic versatility of anaerobic sludge was explored towards waste glycerol valorization. By applying different environmental (methanogenic and sulfate-reducing) conditions, three distinct microbial cultures were obtained from the same inoculum (anaerobic granular sludge), with high microbial specialization, within three different phyla (
Thermodesulfobacteriota
,
Euryarchaeota
and
Pseudomonadota
). The cultures are capable of glycerol conversion through different pathways: (i) glycerol conversion to methane by a bacterium closely related to
Solidesulfovibrio alcoholivorans
(99.8% 16S rRNA gene identity), in syntrophic relationship with
Methanofollis liminatans
(98.8% identity), (ii) fermentation to propionate by
Propionivibrio pelophilus
strain asp66 (98.6% identity), with a propionate yield of 0.88 mmol mmol
−1
(0.71 mg mg
−1
) and a propionate purity of 80–97% and (iii) acetate production coupled to sulfate reduction by
Desulfolutivibrio sulfoxidireducens
(98.3% identity). In conclusion, starting from the same inoculum, we could drive the metabolic and functional potential of the microbiota towards the formation of several valuable products that can be used in industrial applications or as energy carriers.
Key points
Versatility of anaerobic cultures was explored for waste glycerol valorization
Different environmental conditions lead to metabolic specialization
Biocommodities such as propionate, acetate and methane were produced</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>39012392</pmid><doi>10.1007/s00253-024-13248-6</doi><orcidid>https://orcid.org/0000-0002-7999-4620</orcidid><oa>free_for_read</oa></addata></record> |
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| language | eng |
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| source | MEDLINE; SpringerLink Journals; Springer Nature OA Free Journals; Alma/SFX Local Collection |
| subjects | Acetates - metabolism Anaerobic conditions Anaerobic microorganisms Anaerobiosis Bacteria - classification Bacteria - genetics Bacteria - metabolism Biodiesel fuels Biofuels Biomedical and Life Sciences Biotechnology Environmental Biotechnology Environmental conditions Fermentation Glycerol Glycerol - metabolism Industrial applications Inoculum Life Sciences Metabolism Methane Methane - metabolism Microbial Genetics and Genomics Microbiology Microbiota Microorganisms Phylogeny Propionates - metabolism Propionic acid RNA, Ribosomal, 16S - genetics rRNA 16S Sewage - microbiology Sludge Sulfate reduction Sulfates Sulfates - metabolism Versatility |
| title | Metabolic versatility of anaerobic sludge towards platform chemical production from waste glycerol |
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