Comparative Evaluation of the Differentiation and Proliferation Potential of Dental Pulp Stem Cells on Hydroxyapatite/Beta-Tricalcium Bone Graft and Bovine Bone Graft: An In Vitro Study
Stem cells of mesenchymal origin have good proliferative capacity when compared to other stem cell types. Dental pulp stem cells (DPSCs) are a variety of mesenchymal cells obtained from the pulpal tissue of teeth and are abundantly available and easy to obtain. DPSCs facilitate and improve the forma...
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| Veröffentlicht in: | Curēus (Palo Alto, CA) CA), 2024-06, Vol.16 (6), p.e62351 |
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| creator | Shikarkhane, Vangmayee Dodwad, Vidya Bhosale, Nishita Patankar, Swapna A Patankar, Amod Nair, Vivek S |
| description | Stem cells of mesenchymal origin have good proliferative capacity when compared to other stem cell types. Dental pulp stem cells (DPSCs) are a variety of mesenchymal cells obtained from the pulpal tissue of teeth and are abundantly available and easy to obtain. DPSCs facilitate and improve the formation of new bone using different bone graft scaffolds. This present study aims to evaluate and compare the osteogenic potential of DPSCs on alloplastic and xenogeneic bone grafts.
Hydroxyapatite and beta-tricalcium bone graft and bovine bone graft were used in a triplicate manner in the laboratory. DPSCs were obtained from the pulpal tissue of extracted third molars in the laboratory. The cytotoxicity, osteogenic potential, and difference in the rate of proliferation of mesenchymal cells on the biomaterials were assessed.
Darker purple staining was seen in the case of hydroxyapatite/beta-tricalcium bone graft on MTT colorimetric assay stating that there was an increase in cell viability in hydroxyapatite/beta-tricalcium bone graft as compared to the bovine bone graft. Hydroxyapatite/beta-tricalcium bone graft showed more osteogenic potential as compared to the bovine bone graft as a higher degree of red staining was seen in Alizarin staining.
Higher cell viability and higher osteogenic proliferation and differentiation were seen on the hydroxyapatite/beta-tricalcium bone graft compared to the bovine bone scaffold. |
| doi_str_mv | 10.7759/cureus.62351 |
| format | Article |
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Hydroxyapatite and beta-tricalcium bone graft and bovine bone graft were used in a triplicate manner in the laboratory. DPSCs were obtained from the pulpal tissue of extracted third molars in the laboratory. The cytotoxicity, osteogenic potential, and difference in the rate of proliferation of mesenchymal cells on the biomaterials were assessed.
Darker purple staining was seen in the case of hydroxyapatite/beta-tricalcium bone graft on MTT colorimetric assay stating that there was an increase in cell viability in hydroxyapatite/beta-tricalcium bone graft as compared to the bovine bone graft. Hydroxyapatite/beta-tricalcium bone graft showed more osteogenic potential as compared to the bovine bone graft as a higher degree of red staining was seen in Alizarin staining.
Higher cell viability and higher osteogenic proliferation and differentiation were seen on the hydroxyapatite/beta-tricalcium bone graft compared to the bovine bone scaffold.</description><identifier>ISSN: 2168-8184</identifier><identifier>EISSN: 2168-8184</identifier><identifier>DOI: 10.7759/cureus.62351</identifier><identifier>PMID: 39006559</identifier><language>eng</language><publisher>United States: Cureus Inc</publisher><subject>Calcium phosphates ; Cell culture ; Dental pulp ; Dentistry ; Ethics ; Flow cytometry ; Hydroxyapatite ; Penicillin ; Skin & tissue grafts ; Stem cells ; Teeth ; Transplants & implants</subject><ispartof>Curēus (Palo Alto, CA), 2024-06, Vol.16 (6), p.e62351</ispartof><rights>Copyright © 2024, Shikarkhane et al.</rights><rights>Copyright © 2024, Shikarkhane et al. This work is published under https://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>Copyright © 2024, Shikarkhane et al. 2024 Shikarkhane et al.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC11246762/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC11246762/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/39006559$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shikarkhane, Vangmayee</creatorcontrib><creatorcontrib>Dodwad, Vidya</creatorcontrib><creatorcontrib>Bhosale, Nishita</creatorcontrib><creatorcontrib>Patankar, Swapna A</creatorcontrib><creatorcontrib>Patankar, Amod</creatorcontrib><creatorcontrib>Nair, Vivek S</creatorcontrib><title>Comparative Evaluation of the Differentiation and Proliferation Potential of Dental Pulp Stem Cells on Hydroxyapatite/Beta-Tricalcium Bone Graft and Bovine Bone Graft: An In Vitro Study</title><title>Curēus (Palo Alto, CA)</title><addtitle>Cureus</addtitle><description>Stem cells of mesenchymal origin have good proliferative capacity when compared to other stem cell types. Dental pulp stem cells (DPSCs) are a variety of mesenchymal cells obtained from the pulpal tissue of teeth and are abundantly available and easy to obtain. DPSCs facilitate and improve the formation of new bone using different bone graft scaffolds. This present study aims to evaluate and compare the osteogenic potential of DPSCs on alloplastic and xenogeneic bone grafts.
Hydroxyapatite and beta-tricalcium bone graft and bovine bone graft were used in a triplicate manner in the laboratory. DPSCs were obtained from the pulpal tissue of extracted third molars in the laboratory. The cytotoxicity, osteogenic potential, and difference in the rate of proliferation of mesenchymal cells on the biomaterials were assessed.
Darker purple staining was seen in the case of hydroxyapatite/beta-tricalcium bone graft on MTT colorimetric assay stating that there was an increase in cell viability in hydroxyapatite/beta-tricalcium bone graft as compared to the bovine bone graft. Hydroxyapatite/beta-tricalcium bone graft showed more osteogenic potential as compared to the bovine bone graft as a higher degree of red staining was seen in Alizarin staining.
Higher cell viability and higher osteogenic proliferation and differentiation were seen on the hydroxyapatite/beta-tricalcium bone graft compared to the bovine bone scaffold.</description><subject>Calcium phosphates</subject><subject>Cell culture</subject><subject>Dental pulp</subject><subject>Dentistry</subject><subject>Ethics</subject><subject>Flow cytometry</subject><subject>Hydroxyapatite</subject><subject>Penicillin</subject><subject>Skin & tissue grafts</subject><subject>Stem cells</subject><subject>Teeth</subject><subject>Transplants & implants</subject><issn>2168-8184</issn><issn>2168-8184</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><recordid>eNpdkV9PHCEUxYmpqWb1rc-GpC99GWVggJm-NO76NzFxE21fJwwDFcPAlIFN96P124m7tlqfOPndw-FeLgCfSnTMOW1OZAoqTccME1rugH1csrqoy7r68EbvgcNpekQIlYhjxNFHsEcahBilzT74s_DDKIKIZqXg-UrYlKV30GsYHxQ8M1qroFw0WyxcD5fBW5Pplix93JTt85WzLLNaJjvCu6gGuFDWTjDbrtZ98L_XYsy3ojqZqyiK-2CksNKkAc69U_AyCB03T8z9ymTwSr_CUwevHfxhYvA5OvXrA7CrhZ3U4cs5A98vzu8XV8XN7eX14vSmGDEjsaCSy64qOdKMy6bntcacYs1wBrrqOWpwpZq-qzRuiOZEYtnjrla1EFR2ApEZ-LbNHVM3qF7mEYOw7RjMIMK69cK0_1eceWh_-lVblrhiPK9mBr68JAT_K6kptoOZZP4Z4ZRPU0tQjRhhmNFs_fzO-uhTcHm-ZxfjFJFN4NHblv718net5AlvQ6k7</recordid><startdate>20240614</startdate><enddate>20240614</enddate><creator>Shikarkhane, Vangmayee</creator><creator>Dodwad, Vidya</creator><creator>Bhosale, Nishita</creator><creator>Patankar, Swapna A</creator><creator>Patankar, Amod</creator><creator>Nair, Vivek S</creator><general>Cureus Inc</general><general>Cureus</general><scope>NPM</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20240614</creationdate><title>Comparative Evaluation of the Differentiation and Proliferation Potential of Dental Pulp Stem Cells on Hydroxyapatite/Beta-Tricalcium Bone Graft and Bovine Bone Graft: An In Vitro Study</title><author>Shikarkhane, Vangmayee ; 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Dental pulp stem cells (DPSCs) are a variety of mesenchymal cells obtained from the pulpal tissue of teeth and are abundantly available and easy to obtain. DPSCs facilitate and improve the formation of new bone using different bone graft scaffolds. This present study aims to evaluate and compare the osteogenic potential of DPSCs on alloplastic and xenogeneic bone grafts.
Hydroxyapatite and beta-tricalcium bone graft and bovine bone graft were used in a triplicate manner in the laboratory. DPSCs were obtained from the pulpal tissue of extracted third molars in the laboratory. The cytotoxicity, osteogenic potential, and difference in the rate of proliferation of mesenchymal cells on the biomaterials were assessed.
Darker purple staining was seen in the case of hydroxyapatite/beta-tricalcium bone graft on MTT colorimetric assay stating that there was an increase in cell viability in hydroxyapatite/beta-tricalcium bone graft as compared to the bovine bone graft. Hydroxyapatite/beta-tricalcium bone graft showed more osteogenic potential as compared to the bovine bone graft as a higher degree of red staining was seen in Alizarin staining.
Higher cell viability and higher osteogenic proliferation and differentiation were seen on the hydroxyapatite/beta-tricalcium bone graft compared to the bovine bone scaffold.</abstract><cop>United States</cop><pub>Cureus Inc</pub><pmid>39006559</pmid><doi>10.7759/cureus.62351</doi><oa>free_for_read</oa></addata></record> |
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| ispartof | Curēus (Palo Alto, CA), 2024-06, Vol.16 (6), p.e62351 |
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| source | PubMed Central Open Access; PubMed Central |
| subjects | Calcium phosphates Cell culture Dental pulp Dentistry Ethics Flow cytometry Hydroxyapatite Penicillin Skin & tissue grafts Stem cells Teeth Transplants & implants |
| title | Comparative Evaluation of the Differentiation and Proliferation Potential of Dental Pulp Stem Cells on Hydroxyapatite/Beta-Tricalcium Bone Graft and Bovine Bone Graft: An In Vitro Study |
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