Diffusion-driven fed-batch fermentation in perforated ring flasks

Purpose Simultaneous membrane-based feeding and monitoring of the oxygen transfer rate shall be introduced to the newly established perforated ring flask, which consists of a cylindrical glass flask with an additional perforated inner glass ring, for rapid bioprocess development. Methods A 3D-printe...

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Veröffentlicht in:	Biotechnology letters 2024-08, Vol.46 (4), p.571-582
Hauptverfasser:	Lüchtrath, Clara, Lamping, Felix, Hansen, Sven, Finger, Maurice, Magnus, Jørgen, Büchs, Jochen
Format:	Artikel
Sprache:	eng
Schlagworte:	Adapters Ammonium Ammonium sulfate Applied Microbiology Batch Cell Culture Techniques - methods Batch culture Biochemistry Biomedical and Life Sciences Bioreactors Biotechnology Diffusion Diffusion rate E coli Escherichia coli - metabolism Experiments Fed batch Fermentation Flasks Glucose - metabolism Life Sciences Membranes Microbiology Monitoring Original Research Paper Oxygen Oxygen - metabolism Oxygen transfer Printing, Three-Dimensional Reservoirs
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